How to achieve high-level expression of microbial enzymes

Structure-Based Engineering of Methionine Residues in the Catalytic Cores of Alkaline Amylase from Alkalimonas amylolytica for Improved Oxidative Stability

(2012) Haiquan Yang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Strategies for Enhancing Extracellular Secretion of Recombinant Cyclodextrin Glucanotransferase in E. coli

(2012) Berhane Teklehaimanot Tesfai et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Bifidobacterium longum l-Arabinose Isomerase—Overexpression in Lactococcus lactis, Purification, and Characterization

(2012) Noora Salonen et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Lactococcus lactis expressing food-grade β-galactosidase alleviates lactose intolerance symptoms in post-weaning Balb/c mice

(2012) Jingjie Li et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

The metabolic burden of cellulase expression by recombinant Saccharomyces cerevisiae Y294 in aerobic batch culture

(2012) Eugéne van Rensburg et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

High-level expression, purification, and enzymatic characterization of truncated poly(vinyl alcohol) dehydrogenase in methylotrophic yeast Pichia pastoris

(2012) Dongxu Jia et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Improved production of heterologous lipase in Trichoderma reesei by RNAi mediated gene silencing of an endogenic highly expressed gene

(2012) Li-Na Qin et al.   BIORESOURCE TECHNOLOGY

Targeted codon optimization improves translational fidelity for an Fc fusion protein

(2012) Katariina M. Hutterer et al.   BIOTECHNOLOGY AND BIOENGINEERING

Efficient expression and purification of recombinant alcohol oxidase in Pichia pastoris

(2012) Yunping Liu et al.   BIOTECHNOLOGY AND BIOPROCESS ENGINEERING

Tight controlled expression and secretion of Lactobacillus brevis SlpA in Lactococcus lactis

(2012) Axel Hollmann et al.   BIOTECHNOLOGY LETTERS

Extracellular expression and characterization of thermostable lipases, LIP8, LIP14 and LIP18, from Yarrowia lipolytica

(2012) Arti Kumari et al.   BIOTECHNOLOGY LETTERS

Comparative analysis of heterologous expression, biochemical characterization optimal production of an alkaline α-amylase from alkaliphilicAlkalimonas amylolyticainEscherichia coliandPichia pastoris

(2012) Haiquan Yang et al.   BIOTECHNOLOGY PROGRESS

High Growth Rate Downregulates fumA mRNA Transcription but Is Dramatically Compensated by Its mRNA Stability in Escherichia coli

(2012) Hsiao-Hsien Lin et al.   CURRENT MICROBIOLOGY

Engineering the central pathways in Lactococcus lactis: Functional expression of the phosphofructokinase (pfk) and alternative oxidase (aox1) genes from Aspergillus niger in Lactococcus lactis facilitates improved carbon conversion rates under oxidizing conditions

(2012) Maria Papagianni et al.   ENZYME AND MICROBIAL TECHNOLOGY

Expression and production of recombinant cis-epoxysuccinate hydrolase in Escherichia coli under the control of temperature-dependent promoter

(2012) Ziqiang Wang et al.   JOURNAL OF BIOTECHNOLOGY

Enhanced archaeal laccase production in recombinant Escherichia coli by modification of N-terminal propeptide and twin arginine translocation motifs

(2012) Sivakumar Uthandi et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Effective approach to greatly enhancing selective secretion and expression of three cytoplasmic enzymes in Escherichia coli through synergistic effect of EDTA and lysozyme

(2012) Sen-Lin Liu et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Over-expression of a bacterial chitosanase gene in Yarrowia lipolytica and chitosan hydrolysis by the recombinant chitosanase

(2012) Guang-Lei Liu et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Heterologous Expression of Manganese Peroxidase in Aspergillus niger and Its Effect on Phenanthrene Removal from Soil

(2012) Diana V. Cortés-Espinosa et al.   JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY

Optimization of a Heterologous Signal Peptide by Site-Directed Mutagenesis for Improved Secretion of Recombinant Proteins in Escherichia coli

(2012) Mohd Anuar Jonet et al.   JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY

Lactic acid bacteria: a promising alternative for recombinant protein production

(2012) Elena García-Fruitós   Microbial Cell Factories

Construction of a cellulase hyper-expression system in Trichoderma reesei by promoter and enzyme engineering

(2012) Gen Zou et al.   Microbial Cell Factories

Secretory expression and characterization of a novel peroxiredoxin for zearalenone detoxification in Saccharomyces cerevisiae

(2012) Yuqian Tang et al.   MICROBIOLOGICAL RESEARCH

The PurR regulon in Lactococcus lactis - transcriptional regulation of the purine nucleotide metabolism and translational machinery

(2012) C. B. Jendresen et al.   MICROBIOLOGY-SGM

veA-dependent RNA-pol II transcription elongation factor-like protein, RtfA, is associated with secondary metabolism and morphological development in Aspergillus nidulans

(2012) Vellaisamy Ramamoorthy et al.   MOLECULAR MICROBIOLOGY

Trypacidin, a Spore-Borne Toxin from Aspergillus fumigatus, Is Cytotoxic to Lung Cells

(2012) Thierry Gauthier et al.   PLoS One

Fine Tuning of the Lactate and Diacetyl Production through Promoter Engineering in Lactococcus lactis

(2012) Tingting Guo et al.   PLoS One

Exploring Sequence Characteristics Related to High-Level Production of Secreted Proteins in Aspergillus niger

(2012) Bastiaan A. van den Berg et al.   PLoS One

Optimization of a Bacillus sp signal peptide for improved recombinant protein secretion and cell viability in Escherichia coli

(2012) Kheng Oon Low et al.   Bioengineered

Large-scale production of tannase using the yeast Arxula adeninivorans

(2011) Erik Böer et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Transcriptome profiling of degU expression reveals unexpected regulatory patterns in Bacillus megaterium and discloses new targets for optimizing expression

(2011) Claudia Borgmeier et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

The effect of acetylated xylan and sugar beet pulp on the expression and secretion of enzymes by Penicillium purpurogenum

(2011) Mario Navarrete et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Secondary Structure and the Role in Translation Initiation of the 5′-Terminal Region of p53 mRNA

(2011) Leszek Błaszczyk et al.   BIOCHEMISTRY

Overproduction of alkaline polygalacturonate lyase in recombinant Escherichia coli by a two-stage glycerol feeding approach

(2011) Shuying Fang et al.   BIORESOURCE TECHNOLOGY

Special issue on the production of recombinant proteins

(2011) Sergio Sanchez et al.   BIOTECHNOLOGY ADVANCES

Production of recombinant proteins by yeast cells

(2011) Eda Çelik et al.   BIOTECHNOLOGY ADVANCES

Production of recombinant proteins by filamentous fungi

(2011) Owen P. Ward   BIOTECHNOLOGY ADVANCES

A mutant l-asparaginase II signal peptide improves the secretion of recombinant cyclodextrin glucanotransferase and the viability of Escherichia coli

(2011) Noor Faizah Ismail et al.   BIOTECHNOLOGY LETTERS

Evaluation of the catalase promoter for expressing the alkaline xylanase gene (alx) in Aspergillus niger

(2011) Ruchika Sharma et al.   FEMS MICROBIOLOGY LETTERS

Expression of heparan sulfate sulfotransferases in Kluyveromyces lactis and preparation of 3'-phosphoadenosine-5'-phosphosulfate

(2011) X. Zhou et al.   GLYCOBIOLOGY

Improved pectinase production in Penicillium griseoroseum recombinant strains

(2011) J.A. Teixeira et al.   JOURNAL OF APPLIED MICROBIOLOGY

Use of GFP fusions for the isolation of Escherichia coli strains for improved production of different target recombinant proteins

(2011) Sara Alfasi et al.   JOURNAL OF BIOTECHNOLOGY

Efficient expression of a Paenibacillus barcinonensis endoglucanase in Saccharomyces cerevisiae

(2011) María Mormeneo et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Lactococcus lactis M4, a potential host for the expression of heterologous proteins

(2011) Nanyan Noreen et al.   Microbial Cell Factories

Heterologous expression, biochemical characterization, and overproduction of alkaline α-amylase from Bacillus alcalophilus in Bacillus subtilis

(2011) Haiquan Yang et al.   Microbial Cell Factories

Transcriptional regulation of main metabolic pathways of cyoA, cydB, fnr, and fur gene knockout Escherichia coli in C-limited and N-limited aerobic continuous cultures

(2011) Rahul Kumar et al.   Microbial Cell Factories

Chlamydia trachomatis Slc1 is a type III secretion chaperone that enhances the translocation of its invasion effector substrate TARP

(2011) Amanda J. Brinkworth et al.   MOLECULAR MICROBIOLOGY

Construction of two vectors for gene expression in Trichoderma reesei

(2011) Dandan Lv et al.   PLASMID

The Response of Lactococcus lactis to Membrane Protein Production

(2011) Ravi K. R. Marreddy et al.   PLoS One

Recombinant Fab expression and secretion in Escherichia coli continuous culture at medium cell densities: Influence of temperature

(2011) Escarlata Rodríguez-Carmona et al.   PROCESS BIOCHEMISTRY

Co-expression of the Plasmodium falciparum molecular chaperone, PfHsp70, improves the heterologous production of the antimalarial drug target GTP cyclohydrolase I, PfGCHI

(2011) Linda L. Stephens et al.   PROTEIN EXPRESSION AND PURIFICATION

Enhanced Production and Secretion of Heterologous Proteins by the Filamentous Fungus Aspergillus oryzae via Disruption of Vacuolar Protein Sorting Receptor Gene Aovps10

(2010) J. Yoon et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Novel, Versatile, and Tightly Regulated Expression System for Escherichia coli Strains

(2010) Y. J. Choi et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Engineering of protein secretion in yeast: strategies and impact on protein production

(2010) Alimjan Idiris et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Heterologous expression and characterization of a glucose-stimulated β-glucosidase from the termite Neotermes koshunensis in Aspergillus oryzae

(2010) Cristiane Akemi Uchima et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Disruption of ten protease genes in the filamentous fungus Aspergillus oryzae highly improves production of heterologous proteins

(2010) Jaewoo Yoon et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Heterologous expression of a thermophilic esterase in Kluyveromyces yeasts

(2010) Saul Nitsche Rocha et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Purification and Characterization of Termite Endogenous β-1,4-Endoglucanases Produced inAspergillus oryzae

(2010) Kayoko HIRAYAMA et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Detergents enhance EspB secretion from Escherichia coli strains harboring the locus for the enterocyte effacement (LEE) gene

(2010) Noboru Nakasone et al.   FEMS MICROBIOLOGY LETTERS

Heterologous expression of Thermobifida fusca thermostable alpha-amylase in Yarrowia lipolytica and its application in boiling stable resistant sago starch preparation

(2010) Chao-Hsun Yang et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Development of a Bacillus subtilis expression system using the improved Pglv promoter

(2010) Yang M Ming et al.   Microbial Cell Factories

High-throughput recombinant gene expression systems in Pichia pastoris using newly developed plasmid vectors

(2010) Takahiro Sasagawa et al.   PLASMID

Maltose-Binding Protein Enhances Secretion of Recombinant Human Granzyme B Accompanied by In Vivo Processing of a Precursor MBP Fusion Protein

(2010) Benjamin Dälken et al.   PLoS One

Enhanced secretion of recombinant α-cyclodextrin glucosyltransferase from E. coli by medium additives

(2010) Runrong Ding et al.   PROCESS BIOCHEMISTRY

Comparison of different Bacillus subtilis expression systems

(2010) Ľudmila Vavrová et al.   RESEARCH IN MICROBIOLOGY

Contributions of the Pre- and Pro-Regions of a Staphylococcus hyicus Lipase to Secretion of a Heterologous Protein by Bacillus subtilis

(2009) T. R. H. M. Kouwen et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Cellulase Production from Spent Lignocellulose Hydrolysates by Recombinant Aspergillus niger

(2009) B. Alriksson et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Increased product formation induced by a directed secondary substrate limitation in a batch Hansenula polymorpha culture

(2009) Kirsten Kottmeier et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Xplor® 2—an optimized transformation/expression system for recombinant protein production in the yeast Arxula adeninivorans

(2009) Erik Böer et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Delayed supplementation of glycine enhances extracellular secretion of the recombinant α-cyclodextrin glycosyltransferase in Escherichia coli

(2009) Zhaofeng Li et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Enhancement of cell viability and alkaline polygalacturonate lyase production by sorbitol co-feeding with methanol in Pichia pastoris fermentation

(2009) Zhihao Wang et al.   BIORESOURCE TECHNOLOGY

Cloning and Homologous Expression of Novel Lignin Peroxidase Genes in the White-Rot FungusPhanerochaete sordidaYK-624

(2009) Tatsuki SUGIURA et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Production of recombinant proteins by microbes and higher organisms

(2009) Arnold L. Demain et al.   BIOTECHNOLOGY ADVANCES

Heterologous expression of an esterase from Thermus thermophilus HB27 in Saccharomyces cerevisiae

(2009) Olalla López-López et al.   JOURNAL OF BIOTECHNOLOGY

Two-chaperone assisted soluble expression and purification of the bacteriophage T4 long tail fibre protein gp37

(2009) Sergio Galan Bartual et al.   PROTEIN EXPRESSION AND PURIFICATION

TatABC Overexpression Improves Corynebacterium glutamicum Tat-Dependent Protein Secretion

(2008) Y. Kikuchi et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Improving aquaporin Z expression in Escherichia coli by fusion partners and subsequent condition optimization

(2008) Jiazhang Lian et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Enhancement of alkaline polygalacturonate lyase production in recombinant Pichia pastoris according to the ratio of methanol to cell concentration

(2008) Yun Wang et al.   BIORESOURCE TECHNOLOGY

Tuning the expression level of recombinant proteins by modulating mRNA stability in a cell-free protein synthesis system

(2008) Jin-Ho Ahn et al.   BIOTECHNOLOGY AND BIOENGINEERING

Refolding of proteins from inclusion bodies is favored by a diminished hydrophobic effect at elevated pressures

(2008) Ryan L. Crisman et al.   BIOTECHNOLOGY AND BIOENGINEERING

Production of functionally active Penicillium chrysogenum isopenicillin N synthase in the yeast Hansenula polymorpha

(2008) Loknath Gidijala et al.   BMC BIOTECHNOLOGY

A systematic analysis of intronic sequences downstream of 5' splice sites reveals a widespread role for U-rich motifs and TIA1/TIAL1 proteins in alternative splicing regulation

(2008) I. Aznarez et al.   GENOME RESEARCH

Solubilization at high pH results in improved recovery of proteins from inclusion bodies ofE. coli

(2008) Surinder M. Singh et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Immobilized chaperones: A productive alternative to refolding of bacterial inclusion body proteins

(2008) Kamna Jhamb et al.   PROCESS BIOCHEMISTRY

Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study

(2008) Nicola A. Burgess-Brown et al.   PROTEIN EXPRESSION AND PURIFICATION

An improved SUMO fusion protein system for effective production of native proteins

(2008) Chien-Der Lee et al.   PROTEIN SCIENCE

Sustained expression of keratinase gene under PxylA and PamyL promoters in the recombinant Bacillus megaterium MS941

(2007) S. Radha et al.   BIORESOURCE TECHNOLOGY

L-Tyrosine production by recombinantEscherichia coli: Fermentation optimization and recovery

(2007) Ranjan Patnaik et al.   BIOTECHNOLOGY AND BIOENGINEERING

A Facile and Efficient Method To Achieve LacZ Overproduction by the Expression Vector Carrying the Thermoregulated Promoter and Plasmid Copy Number

(2004) Yun-Peng Chao et al.   BIOTECHNOLOGY PROGRESS