Article
Multidisciplinary Sciences
Seol Hwa Jo, Woo Hang Heo, Hye-Youn Son, Mingji Quan, Bok Sil Hong, Ju Hee Kim, Han-Byoel Lee, Wonshik Han, Yeonju Park, Dong-Sup Lee, Nam Hoon Kwon, Min Chul Park, Jeesoo Chae, Jong-Il Kim, Dong-Young Noh, Hyeong-Gon Moon
Summary: The direct co-culture of breast cancer cells with non-tumorigenic mammary epithelial cells leads to substantial changes in the molecular and functional characteristics of the mammary epithelial cells, with enhanced proliferation, migration, colony formation, and sphere formation capabilities. Dysregulation of cancer-related pathways is observed, along with upregulation of S100A8 and S100A9, suggesting a potential transition program mediated by these proteins in non-tumorigenic epithelial cells.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Jinglin Zhu, Ruifei Huang, Ruijie Yang, Yue Xiao, Jiangna Yan, Chunli Zheng, Wei Xiao, Chao Huang, Yonghua Wang
Summary: The study found that licorice-induced cell cycle arrest of tumor cells is related to down-regulation of the CDK4-Cyclin D1 complex, leading to increased PD-L1 protein abundance. In vivo studies demonstrated that licorice's inhibition of NSCLC tumor growth relies on increased antigen presentation and improved CD8(+) T cell infiltration.
CANCER CELL INTERNATIONAL
(2021)
Article
Biotechnology & Applied Microbiology
Chiung Wen Kuo, Feby Wijaya Pratiwi, Yen-Ting Liu, Di-Yen Chueh, Peilin Chen
Summary: Validating the design of nanomaterials in disease models is crucial for the development of bioinspired nanomaterials for therapeutic applications. In this study, a straightforward technique using a two-photon microscope synchronized with electrocardiogram signals was developed for long-term real-time imaging of contracting hearts at the subcellular level. The results showed that the structural and dynamic behaviors of organelles in cardiomyocytes were closely related to heart function. In a myocardial infarction model, impaired cardiac function resulted in decreased sarcomere shortening and increased distances between sarcomeres, along with significant remodeling of the T-tubule structures.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Immunology
Yoshinobu Konishi, Kenta Terai
Summary: Inflammation plays a role in cancer development and progression, and the complex intercellular cross-talks in the tumor microenvironment have not been successfully replicated in vitro. Two-photon excitation microscopes (2P-IVM) with fluorescent indicators are currently used to observe intercellular cross-talks, revealing the cellular and molecular mechanisms in a spatiotemporal context. This review summarizes the current state of 2P-IVM and discusses the potential application of red-shifted indicators and optogenetic tools. In the era of single-cell transcriptomics and data-driven research, 2P-IVM remains crucial in providing the missing spatiotemporal context in cancer research.
INFLAMMATION AND REGENERATION
(2023)
Review
Immunology
Jorge Almagro, Hendrik A. A. Messal
Summary: Volume imaging technologies, such as tissue clearing and intravital microscopy, have greatly advanced our understanding of cancer-TME interactions and have been instrumental in fundamental cancer research and clinical oncology.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Agatha M. Reigoto, Sarah A. Andrade, Marianna C. R. R. Seixas, Manoel L. Costa, Claudia Mermelstein
Summary: Microscopy plays a crucial role in visualizing and studying the structure and function of cells in biomedical research. The usage of different microscopy techniques varies significantly between fields such as pharmacology and cell biology. While nearly half of articles in pharmacology journals did not utilize any microscopy method, almost all articles in cell biology journals incorporated microscopy. The most common microscopy methods across all fields were bright field and fluorescence (conventional and confocal).
Article
Multidisciplinary Sciences
George Alzeeb, Danielle Arzur, Valerie Trichet, Matthieu Talagas, Laurent Corcos, Catherine Le Jossic-Corcos
Summary: Gastric cancer is the third leading cause of cancer-related mortality worldwide, often diagnosed at advanced stages. Utilizing the cholesterol-lowering drug lovastatin as an adjuvant to chemotherapy has shown significant efficacy in treating gastric cancer. In a 3D model, the combination of lovastatin and docetaxel effectively eliminates growing gastric cancer cells.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Yi Chen, Shuwen You, Jie Li, Yifan Zhang, Georgia Kokaraki, Elisabeth Epstein, Joseph Carlson, Wen-Kuan Huang, Felix Haglund
Summary: Through studying the transcriptome features of endometrial cancer patients, we identified T follicular helper-like cells (Tfh) as an important factor with potential clinical application in anti-PD-1 treatment. We constructed a Tfh Infiltration Risk Model (TIRM) and found that it outperformed existing models in predicting the prognosis of EC patients.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Chao Yang, Haoqiang Zhan, Yiqing Zhao, Yasong Wu, Lisha Li, Heping Wang
Summary: Through studying the role of MEX3A in glioma, this research found that it may promote glioma development by regulating cell proliferation, apoptosis, cycle, and migration, with CCL2 being a potential downstream target. These findings reveal a new therapeutic target for treating glioma.
CELL DEATH & DISEASE
(2021)
Review
Immunology
Xuwen Peng, Yuke Wang, Jie Zhang, Zhihong Zhang, Shuhong Qi
Summary: Cancer immunotherapy has promising potential and understanding the interactions between immune cells and tumor cells in the tumor microenvironment is crucial. Intravital imaging has emerged as a powerful tool for visualizing dynamic processes and functions of immune cells, aiding in the development of more effective cancer immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Yue Wang, Xueer Zhou, Wenxuan Wang, Yongyao Wu, Zhiyong Qian, Qiang Peng
Summary: Cancer poses a serious threat to humans, and traditional therapies may not be sufficient for the increasing demands of patients. NaHCO3 has been found to potentially treat cancer by reversing the acidic tumor microenvironment.
CHINESE CHEMICAL LETTERS
(2021)
Article
Chemistry, Analytical
Hua Tian, Louis J. Sparvero, Tamil Selvan Anthonymuthu, Wan-Yang Sun, Andrew A. Amoscato, Rong-Rong He, Hulya Bayr, Valerian E. Kagan, Nicholas Winograd
Summary: This study developed a new methodology for comprehensive lipidomic and metabolomic profiling of individual cells on frozen-hydrated tissue sections, combined with cell-type specific lanthanide antibodies analysis. The research revealed distinct variations of distribution and intensities of key ions in different types of cells within the tumor microenvironment. The feasibility of employing SIMS imaging for multi-omics profiling at the single-cell level in the same tissue section opens up possibilities for gaining new insights into cellular interactions in various tissue microenvironments.
ANALYTICAL CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Gayathri Ashok, Sudha Ramaiah
Summary: In this study, an integrated systems biology approach was used to identify major genes and pathways associated with metastasis in ccRCC, providing important clinical implications for theranostics application.
JOURNAL OF GENE MEDICINE
(2023)
Article
Oncology
Hanwen Wang, Huilin Ma, Richard J. Sove, Leisha A. Emens, Aleksander S. Popel
Summary: This study introduces a modular quantitative systems pharmacology (QSP) platform for predicting immunotherapy efficacy and identifying predictive biomarkers. Virtual clinical trials were conducted using a virtual patient cohort generated by the model, with retrospective analysis and model validation based on clinical trial data.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Biotechnology & Applied Microbiology
Qiaoqi Chen, Liang Zhang, Lin Li, Mixiao Tan, Weiwei Liu, Shuling Liu, Zhuoyan Xie, Wei Zhang, Zhigang Wang, Yang Cao, Tingting Shang, Haitao Ran
Summary: The combination of multiple therapeutic modalities in nano-targeted cell-based cocktail therapy shows promise in promoting tumor regression and combating metastasis/recurrence. This approach not only effectively targets tumor sites, but also triggers photothermal effects, induces in situ tumor eradication, enhances DC maturation, polarizes TAMs, and increases CTL infiltration, ultimately leading to significant inhibition of primary tumors and metastasis.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Oncology
Mikala Egeblad
Summary: Dr. Zena Werb, a prominent faculty member at UCSF for over 40 years, was well-known for her discoveries in matrix metalloproteinases and their impact on development and disease. Her work significantly contributed to the understanding of the tumor microenvironment, making her a thought leader in the field and mentor to many researchers.
ANNUAL REVIEW OF CANCER BIOLOGY, VOL 5, 2021
(2021)
Article
Oncology
P. Grivas, A. R. Khaki, T. M. Wise-Draper, B. French, C. Hennessy, C-Y Hsu, Y. Shyr, X. Li, T. K. Choueiri, C. A. Painter, S. Peters, B. Rini, M. A. Thompson, S. Mishra, D. R. Rivera, J. D. Acoba, M. Z. Abidi, Z. Bakouny, B. Bashir, T. Bekaii-Saab, S. Berg, E. H. Bernicker, M. A. Bilen, P. Bindal, R. Bishnoi, N. Bouganim, D. W. Bowles, A. Cabal, P. F. Caimi, D. D. Chism, J. Crowell, C. Curran, A. Desai, B. Dixon, D. B. Doroshow, E. B. Durbin, A. Elkrief, D. Farmakiotis, A. Fazio, L. A. Fecher, D. B. Flora, C. R. Friese, J. Fu, S. M. Gadgeel, M. D. Galsky, D. M. Gill, M. J. Glover, S. Goyal, P. Grover, S. Gulati, S. Gupta, S. Halabi, T. R. Halfdanarson, B. Halmos, D. J. Hausrath, J. E. Hawley, E. Hsu, M. Huynh-Le, C. Hwang, C. Jani, A. Jayaraj, D. B. Johnson, A. Kasi, H. Khan, V. S. Koshkin, N. M. Kuderer, D. H. Kwon, P. E. Lammers, A. Li, A. Loaiza-Bonilla, C. A. Low, M. B. Lustberg, G. H. Lyman, R. R. McKay, C. McNair, H. Menon, R. A. Mesa, V Mico, D. Mundt, G. Nagaraj, E. S. Nakasone, J. Nakayama, A. Nizam, N. L. Nock, C. Park, J. M. Patel, K. G. Patel, P. Peddi, N. A. Pennell, A. J. Piper-Vallillo, M. Puc, D. Ravindranathan, M. E. Reeves, D. Y. Reuben, L. Rosenstein, R. P. Rosovsky, S. M. Rubinstein, M. Salazar, A. L. Schmidt, G. K. Schwartz, M. R. Shah, S. A. Shah, C. Shah, J. A. Shaya, S. R. K. Singh, M. Smits, K. E. Stockerl-Goldstein, D. G. Stover, M. Streckfuss, S. Subbiah, L. Tachiki, E. Tadesse, A. Thakkar, M. D. Tucker, A. K. Verma, D. C. Vinh, M. Weiss, J. T. Wu, E. Wulff-Burchfield, Z. Xie, P. P. Yu, T. Zhang, A. Y. Zhou, H. Zhu, L. Zubiri, D. P. Shah, J. L. Warner, GdL Lopes
Summary: This study analyzed clinical factors and laboratory measurements of cancer patients with COVID-19, finding that factors such as age, sex, comorbidities, cancer type, and laboratory results were associated with COVID-19 severity. Patients diagnosed early in the pandemic had worse outcomes, and specific anticancer therapies may increase 30-day all-cause mortality. More research is needed to confirm these findings and caution may be needed in using certain anticancer treatments.
ANNALS OF ONCOLOGY
(2021)
Meeting Abstract
Oncology
Elizabeth Trice Loggers, Elizabeth Marie Wulff-Burchfield, Ishwaria Mohan Subbiah, Ali Raza Khaki, Pamela Egan, Dimitrios Farmakiotis, Hardeep Phull, Elizabeth Nakasone, Chris Labaki, Peter Paul Yu, Monika Joshi, Elizabeth A. Griffiths, Trisha Michel Wise-Draper, Chinmay Jani, Astha Thakkar, Matthew Puc, Clara Hwang, Blanche H. Mavromatis, Dimpy P. Shah, Jeremy Lyle Warner
JOURNAL OF CLINICAL ONCOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Nicole D. Barth, Lorena Mendive-Tapia, Ramon Subiros-Funosas, Ouldouz Ghashghaei, Rodolfo Lavilla, Laura Maiorino, Xue-Yan He, Ian Dransfield, Mikala Egeblad, Marc Vendrell
Summary: The study describes the design and optimization of a fluorescent probe, Apotracker Red, for real-time detection of cancer cell death. The probe exhibits strong fluorogenic behavior, high selectivity, and excellent stability, making it a reliable optical reporter for characterizing the effects of anticancer drugs in cells in vitro and for direct imaging of chemotherapy-induced apoptosis in vivo in mouse models of breast cancer.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Pathology
Laura Maiorino, Juliane Dassler-Plenker, Lijuan Sun, Mikala Egeblad
Summary: Chronic inflammation increases the risk of various cancers, while tumors themselves can trigger an inflammatory response. Inflammation can directly impact tumor growth and influence adaptive immune responses. Innate immune cells exhibit heterogeneity and plasticity, with variable phenotypes depending on tumor type, stage, and treatment options.
ANNUAL REVIEW OF PATHOLOGY-MECHANISMS OF DISEASE
(2022)
Article
Multidisciplinary Sciences
Laura Maiorino, Margaret Shevik, Jose M. Adrover, Xiao Han, Elias Georgas, John Erby Wilkinson, Harrison Seidner, Leonie Foerschner, David A. Tuveson, Yi-Xian Qin, Mikala Egeblad
Summary: Intravital microscopy (IVM) allows visualization of cell movement, division, and death at single-cell resolution. Thin film polydimethylsiloxane (PDMS) windows offer a simpler and less inflammatory alternative to traditional glass windows, allowing longitudinal imaging and tracking of the same tissue region using embedded grids.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Medicine, Research & Experimental
Jose M. Adrover, Lucia Carrau, Juliane Dassler-Plenker, Yaron Bram, Vasuretha Chandar, Sean Houghton, David Redmond, Joseph R. Merrill, Margaret Shevik, Benjamin R. TenOever, Scott K. Lyons, Robert E. Schwartz, Mikala Egeblad
Summary: Severe acute lung injury, characterized by high mortality and limited treatment options, can be improved with the use of disulfiram, an FDA-approved drug for alcohol use disorder. Disulfiram reduces neutrophil extracellular traps (NETs), improves survival and lung function in a mouse model of transfusion-related acute lung injury, and shows potential benefits in reducing NETs and inflammation in SARS-CoV-2-infected hamsters.
Review
Oncology
Xue-Yan He, David Ng, Mikala Egeblad
Summary: The article introduces the roles of NETs in cancer, including promoting tumor growth, influencing immune escape, and promoting metastasis. It also discusses the potential clinical implications of targeting NETs as a novel therapeutic strategy for cancer.
ANNUAL REVIEW OF CANCER BIOLOGY
(2022)
Review
Immunology
Daniela F. Quail, Borko Amulic, Monowar Aziz, Betsy J. Barnes, Evgeniy Eruslanov, Zvi G. Fridlender, Helen S. Goodridge, Zvi Granot, Andres Hidalgo, Anna Huttenlocher, Mariana J. Kaplan, Ilaria Malanchi, Taha Merghoub, Etienne Meylan, Vivek Mittal, Mikael J. Pittet, Andrea Rubio-Ponce, Irina A. Udalova, Timo K. van den Berg, Denisa D. Wagner, Ping Wang, Arturo Zychlinsky, Karin E. de Visser, Mikala Egeblad, Paul Kubes
Summary: This article discusses the functional states and heterogeneity of neutrophils in cancer and provides a consensus statement on the controversies and solutions in neutrophil research. Further research is needed to understand the role of neutrophils in cancer and explore potential novel opportunities for cancer treatment.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Xiaoli S. Wu, Xue-Yan He, Jonathan J. Ipsaro, Yu-Han Huang, Jonathan B. Preall, David Ng, Yan Ting Shue, Julien Sage, Mikala Egeblad, Leemor Joshua-Tor, Christopher R. Vakoc
Summary: Tuft cells are rare chemosensory cells that play a role in coordinating immune and neural responses to foreign pathogens. Recent studies have shown their involvement in small-cell lung cancer. This study reveals the critical role of POU2F3-OCA-T complex in tuft cell identity and its potential as a molecular vulnerability in small-cell lung cancer.
Article
Medicine, Research & Experimental
Dongyan Song, Jose M. Adrover, Christy Felice, Lisa N. Christensen, Xue-Yan He, Joseph R. Merrill, John E. Wilkinson, Tobias Janowitz, Scott K. Lyons, Mikala Egeblad, Nicholas K. Tonks
Summary: PTP1B inhibitors have shown potential in preventing acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). These inhibitors can attenuate aberrant neutrophil function, inhibit neutrophil migration, and promote an aged-neutrophil phenotype by reducing signaling through the CXCR4 chemokine receptor. Additionally, PTP1B inhibitors have therapeutic effects in sepsis models.
Article
Oncology
Michael J. Wagner, Cassandra Hennessy, Alicia Beeghly, Benjamin French, Dimpy P. Shah, Sarah Croessmann, Diana Vilar-Compte, Erika Ruiz-Garcia, Matthew Ingham, Gary K. Schwartz, Corrie A. Painter, Rashmi Chugh, Leslie Fecher, Cathleen Park, Olga Zamulko, Jonathan C. Trent, Vivek Subbiah, Ali Raza Khaki, Lisa Tachiki, Elizabeth S. Nakasone, Elizabeth T. Loggers, Chris Labaki, Renee Maria Saliby, Rana R. McKay, Archana Ajmera, Elizabeth A. Griffiths, Igor Puzanov, William D. Tap, Clara Hwang, Sachin R. Jhawar, Brandon Hayes-Lattin, Elizabeth Wulff-Burchfield, Anup Kasi, Daniel Y. Reuben, Gayathri Nagaraj, Monika Joshi, Hyma Polimera, Amit A. Kulkarni, Khashayar Esfahani, Daniel H. Kwon, Luca Paoluzzi, Mehmet A. Bilen, Eric B. Durbin, Petros Grivas, Jeremy L. Warner, Elizabeth J. Davis
Summary: Patients with sarcoma have a higher risk of developing complications from COVID-19, and patients with bone sarcoma may be at a greater risk of death from COVID-19. Older age, poor performance status, and lung metastases are risk factors for more severe COVID-19.
Review
Oncology
Jose M. Adrover, Sheri A. C. McDowell, Xue-Yan He, Daniela F. Quail, Mikala Egeblad
Summary: Neutrophils are key players in the immune system, involved in pathogen eradication and cancer progression. Cancer cells manipulate neutrophil biology, including the formation of neutrophil extracellular traps (NETs), to their advantage. This article discusses how cancer stimulates NET formation and how NETs contribute to disease progression. It also explores the link between neutrophils, NETs, vascular dysfunction, thrombosis, and increased cardiovascular risk in cancer patients. Therapeutic strategies targeting NETs in clinical settings are proposed.
Article
Biochemical Research Methods
Lijuan Sun, Xiao Han, Mikala Egeblad
Summary: An in vitro co-culture system was established using MPLA+IFNγ-treated TAMs to kill cancer cells. The technical challenge lies in isolating and staining TAMs and cancer cells from the same tumor.
Article
Oncology
Takeo Fujii, Hasan Rehman, Su Yun Chung, Janice Shen, James Newman, Vernon Wu, Adam Hines, Elham Azimi-Nekoo, Fatima Fayyaz, Meeyoung Lee, George Raptis, Mikala Egeblad, Xinhua Zhu
Summary: The study found that G-CSF use was not associated with an increased risk of brain metastasis in patients with de novo Stage IV breast cancer. However, pulmonary embolism/deep venous thrombosis may be related to brain metastasis. Further research is needed to determine if DVT/PE with or without elevated NETs levels in the blood can predict the development of brain metastasis in these patients.