Article
Pharmacology & Pharmacy
Ziying Sun, Qianqian Liu, Zhongyang Lv, Jiawei Li, Xingquan Xu, Heng Sun, Maochun Wang, Kuoyang Sun, Tianshu Shi, Zizheng Liu, Guihua Tan, Wenqiang Yan, Rui Wu, Yannick Xiaofan Yang, Shiro Ikegawa, Qing Jiang, Yang Sun, Dongquan Shi
Summary: The study revealed that inhibiting M1 macrophage polarization can slow down the progression of osteoarthritis, with SHP099 potentially being a promising therapeutic strategy for OA treatment.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Immunology
Bart M. Tiemeijer, Sebastiaan Heester, Ashley Y. W. Sturtewagen, Anthal I. P. M. Smits, Jurjen Tel
Summary: In this study, we used a droplet approach to investigate single-cell macrophage activation and the impact of cellular communication on macrophage-mediated inflammatory homeostasis. We found that only a fraction of macrophages can produce IL-10 in addition to TNF alpha upon stimulation, and these cells are not phenotypically different from IL-10 non-producers. Furthermore, we discovered that the dynamics of TNF alpha and IL-10 are controlled by macrophage density. These findings highlight the significance of understanding the complex communication between macrophages and other immune cells and its contribution to population-wide immune responses.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Gastroenterology & Hepatology
Lijuan Wang, Ning Zhang, Dianwen Han, Peng Su, Bing Chen, Wenjing Zhao, Ying Liu, Hanwen Zhang, Guohong Hu, Qifeng Yang
Summary: The study revealed that MTDH expression in colonic lamina propria macrophages is correlated with the severity of inflammatory colitis. MTDH-/- mice were protected against DSS-induced colitis, but adoptive transfer of MTDH wild-type monocytes partially restored susceptibility. TLR stimulation induced MTDH expression and absence of MTDH suppressed inflammatory cytokine production by macrophages.
JOURNAL OF CROHNS & COLITIS
(2021)
Article
Multidisciplinary Sciences
Alice Coillard, Lea Guyonnet, Alba De Juan, Adeline Cros, Elodie Segura
Summary: Monocytes are recruited to inflamed tissues where they encounter a broad range of microbial motifs, leading to differentiation into mo-mac or mo-DC. Viruses promote mo-mac differentiation via TLR signaling, while Mycobacteria favor mo-DC differentiation through NOD signaling.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Engineering, Biomedical
Jeremy M. M. Lowen, Gabriella C. C. Bond, Katherine H. H. Griffin, Nathan K. K. Shimamoto, Victoria L. L. Thai, J. Kent Leach
Summary: Microgels with inherent porosity and tunability are becoming a promising platform for in vitro models and guiding cell fate. This study presents a light-based technique for rapidly annealing microgels of varying diameters, controlled by the number of arms available for crosslinking, functionalization, and annealing. The impact of microgel diameter on void space, porosity, cell spreading, cell aggregation, and macrophage polarization is explored. The use of UV light enables noninvasive injection and fast annealing, making this approach clinically relevant and highly tunable.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Cell Biology
Jeremy A. Pike, Victoria A. Simms, Christopher W. Smith, Neil V. Morgan, Abdullah O. Khan, Natalie S. Poulter, Iain B. Styles, Steven G. Thomas
Summary: The assessment of platelet spreading and quantification through light microscopy is crucial for platelet biologists. The presented workflow uses image segmentation and machine learning to analyze a large number of cells efficiently. The method is freely available and implemented using open-source software.
Article
Immunology
Anna J. Pietrobon, Fabio S. Y. Yoshikawa, Luana M. Oliveira, Natalli Z. Pereira, Tais Matozo, Bruna C. de Alencar, Alberto J. S. Duarte, Maria N. Sato
Summary: Cord blood macrophages are more susceptible to HIV-1 infection, but treatment with CL097 can enhance their antiviral response and inhibit viral replication. This finding may have potential significance in enhancing neonatal immune response.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Microbiology
Yajie Chen, Henghui He, Licheng Luo, Kangyi Liu, Min Jiang, Shiqi Li, Xianqi Zhang, Xin Yang, Qian Liu
Summary: A novel method utilizing artificial intelligence techniques for analyzing fibro-pathological images allows for standardized, precise, and staged diagnosis of pulmonary fibrosis. Analysis of COVID-19 lung tissue microscopic images revealed a significant correlation between the number of macrophage aggregates and the degree of fibrosis, particularly the M2-type macrophages. This method advancement can contribute to the understanding and prevention of pulmonary fibrosis, and further exploration of the specific molecular mechanisms of M2-type macrophage polarization.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Tohn Borjigin, Anuraag Boddupalli, Millicent O. Sullivan
Summary: This study developed a new algorithm for accurate counting and distinguishing of different cell types in cell image analysis. The algorithm differentiated macrophages and fibroblasts based on differences in cell height from the background. It accounted for variations in cell size/structure and high-density seeding conditions, and also addressed non-idealities in cell structures. An isolation algorithm was used for analyzing coculture environments. The algorithm achieved accurate cell counting within a small error margin for both monocultured and cocultured cells.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Biochemistry & Molecular Biology
Miguel A. Idoate Gastearena, Alvaro Lopez-Janeiro, Arturo Lecumberri Aznarez, Inigo Arana-Iniguez, Francisco Guillen-Grima
Summary: Immunostimulatory therapies using immune checkpoint blockers show clinical activity in a subset of glioblastoma (GBM) patients. The tumor immune microenvironment and immune biomarkers play important roles in the immune response to GBM. The expression of CD8 is positively correlated with lymphocyte immunosuppression mechanisms and other immune biomarkers. High expressions of CD8 and CD163 are associated with shorter survival durations.
Article
Biochemistry & Molecular Biology
Mary-Keara Boss, Remy Watts, Lauren G. Harrison, Sophie Hopkins, Lyndah Chow, Erin Trageser, Carina Easton, Susan M. LaRue, Daniel Regan, Mark W. Dewhirst, Steven Dow
Summary: This study investigated the immune responses within the tumor microenvironment (TME) following stereotactic body radiotherapy (SBRT) and the effects of local injection of immune modulatory antibodies on those responses using a canine spontaneous tumor model. The results showed that SBRT alone increased the density of immunosuppressive cells in the tumor, while the addition of immune therapy reduced these cells. Furthermore, increased concentrations of immune-related factors were observed in the serum.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Bikash Thapa, Seongwon Pak, Dohyeon Chung, Hye Kyoung Shin, Seong Ho Lee, Keunwook Lee
Summary: This study evaluated the immunomodulatory potential of a TLR inhibitor peptide in an animal model of ulcerative colitis. The peptide suppressed the production of pro-inflammatory cytokines in macrophages and ameliorated colitis symptoms in mice. It also enhanced the differentiation of monocyte-driven macrophages into regulatory macrophages and suppressed the activation of Th17 cells. Therefore, the TLR inhibitor peptide shows promise as a novel therapeutic candidate for the treatment of inflammatory bowel disease.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
John P. Crimaldi, Aaron C. True, Karl G. Linden, Mark T. Hernandez, Lars T. Larson, Anna K. Pauls
Summary: This study investigates the kinematics and velocity fields of aerosol plumes emitted from flushed toilets using laser technology. The findings reveal that flushed toilets generate strong chaotic jets that propel aerosol plumes to heights of up to 1.5 meters within 8 seconds. This research provides valuable insights for future toilet design and pathogen disinfection strategies.
SCIENTIFIC REPORTS
(2022)
Article
Fisheries
Tan Phat Nguyen, Bao Trung Nguyen, Fan-Hua Nan, Meng-Chou Lee, Po-Tsang Lee
Summary: Nile tilapia (Oreochromis niloticus) is an important food fish species mainly cultivated in tropical and subtropical countries. This study focuses on Toll-like receptors (TLRs) and the clonation of OnTLR23 in fish cells. The study also explores the interaction of OnTLR23 with other molecules and its potential role in immune responses.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Geochemistry & Geophysics
Eoghan P. Holohan, Sam Poppe, Audray Delcamp, Paul K. Byrne, Thomas R. Walter, Benjamin van Wyk de Vries, Matthieu Kervyn
Summary: Many volcanoes show evidence of long-term deformation driven by gravity, which affects the development of magmatic plumbing systems and volcanic vent locations. This deformation can manifest as earthquakes and ground surface motions. By revisiting classic experiments and using digital imaging techniques, it has been observed that volcano deformation can transition from sagging to spreading as the volcano grows.
EARTH AND PLANETARY SCIENCE LETTERS
(2023)
Article
Critical Care Medicine
Alan Benard, Imme Sakwa, Pablo Schierloh, Andre Colom, Ingrid Mercier, Ludovic Tailleux, Luc Jouneau, Pierre Boudinot, Talal Al-Saati, Roland Lang, Jan Rehwinkel, Andre G. Loxton, Stefan H. E. Kaufmann, Veronique Anton-Leberre, Anne O'Garra, Maria Del Carmen Sasiain, Brigitte Gicquel, Simon Fillatreau, Olivier Neyrolles, Denis Hudrisier
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2018)
Article
Biochemistry & Molecular Biology
Bea Unterer, Veit Wiesmann, Mekala Gunasekaran, Heinrich Sticht, Clara Tenkerian, Juergen Behrens, Marina Leone, Felix B. Engel, Nathalie Britzen-Laurent, Elisabeth Naschberger, Thomas Wittenberg, Michael Stuerzl
BIOCHEMICAL JOURNAL
(2018)
Article
Gastroenterology & Hepatology
Peter Klare, Christoph Sander, Martin Prinzen, Bernhard Haller, Sebastian Nowack, Mohamed Abdelhafez, Alexander Poszler, Hayley Brown, Dirk Wilhelm, Roland M. Schmid, Stefan von Delius, Thomas Wittenberg
GASTROINTESTINAL ENDOSCOPY
(2019)
Letter
Biochemical Research Methods
Benjamin Schmid, Philipp Tripal, Tina Fraass, Christina Kersten, Barbara Ruder, Anika Grueneboom, Jan Huisken, Ralf Palmisano
Article
Medicine, Research & Experimental
Markus Eckstein, Verena Sailer, Boye Schnack Nielsen, Thomas Wittenberg, Veit Wiesmann, Verena Lieb, Elke Nolte, Arndt Hartmann, Glen Kristiansen, Nicolas Wernert, Bernd Wullich, Helge Taubert, Sven Wach
LABORATORY INVESTIGATION
(2019)
Editorial Material
Biochemistry & Molecular Biology
Rafael Pulido, Roland Lang
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Biology
Johannes Maier, Jerome Perret, Michaela Huber, Martina Simon, Stephanie Schmitt-Rueth, Thomas Wittenberg, Christoph Palm
COMPUTERS IN BIOLOGY AND MEDICINE
(2019)
Article
Materials Science, Characterization & Testing
Roland Gruber, Stefan Gerth, Joelle Claussen, Norbert Woerlein, Norman Uhlmann, Thomas Wittenberg
Summary: XXL-CT can produce large scale volume datasets of scanned objects, but traditional automatic or manual segmentation is not feasible due to the complexity and unknown properties. Using segmentation algorithms for interactive and partial segmentation can assist in exploring and understanding such large scale volume data.
JOURNAL OF NONDESTRUCTIVE EVALUATION
(2021)
Article
Agronomy
Stefan Gerth, Joelle Claussen, Anja Eggert, Norbert Worlein, Michael Waininger, Thomas Wittenberg, Norman Uhlmann
Summary: The RootForce approach proposed in this study enables semi-automatic and robust root system segmentation from CTX data, providing a more efficient assessment of root architectures of different types of plants.
Article
Biochemical Research Methods
Benjamin Schmid, Philipp Tripal, Zoltan Winter, Ralph Palmisano
Summary: Creating 3D animations from microscopy data is computationally expensive. 3Dscript.server is a software that runs on dedicated shared workstations, offering unique rendering features and intuitive animation description. It eliminates the need for hardware requirements on the client-side.
Review
Dentistry, Oral Surgery & Medicine
Gyoergy Wersenyi, Verena Scheper, Simone Spagnol, Thomas Eixelberger, Thomas Wittenberg
Summary: This paper reviews the current possibilities and latest use cases for 3D scanning, data processing, and printing of outer ear replicas, with a focus on low-cost solutions for rehabilitation engineering.
HEAD & FACE MEDICINE
(2023)
Article
Biology
Christine Mau, Heike Rudolf, Frederic Strobl, Benjamin Schmid, Timo Regensburger, Ralf Palmisano, Ernst H. K. Stelzer, Leila Taher, Ezzat El-Sherif
Summary: This article addresses the question of how genes are regulated in terms of timing and spatial pattern during development. By studying the AP patterning of the beetle Tribolium, the authors established a model system to investigate dynamic and temporal pattern formation at the enhancer level. They discovered dynamic enhancers and static enhancers that play a balancing role in regulating gene expression during embryonic pattern formation.
Article
Medicine, General & Internal
Michael Knott, Philip Hoelter, Liam Soder, Sven Schlaffer, Sophia Hoffmanns, Roland Lang, Arnd Doerfler, Manuel Alexander Schmidt
Summary: This study evaluated the relationship between brain tissue oxygenation status measured with DSC-PWI and microbiological/inflammatory status. The results showed that relative cerebral blood flow, relative cerebral blood volume, and regional cerebral metabolic rate for oxygen were significantly lower in the oedema around abscesses without microbiological evidence, compared to microbiological positive lesions.
Review
Gastroenterology & Hepatology
Thomas Wittenberg, Martin Raithel
Article
Chemistry, Physical
Joe Harris, Ingo P. Mey, Corinna F. Boehm, Thi Thanh Huyen Trinh, Simon Leupold, Carsten Prinz, Philipp Tripal, Ralf Palmisano, Stephan E. Wolf
NANOSCALE HORIZONS
(2019)