Article
Immunology
Matthew J. Wiest, Laurie Baert, Chao Gu, Kevin M. Gayler, Hyoungjun Ham, Laurent Gorvel, Mira T. Keddis, Leroy W. Griffing, Hyemee Joo, Jean-Pierre Gorvel, Daniel D. Billadeau, Robert R. Kane, Sangkon Oh
Summary: This study investigated the mechanisms of cytokine expression mediated by TLR7 in pDCs and found that specific intracellular trafficking pathways can regulate the production of IFN alpha and TNF alpha by pDCs. This has important implications for understanding the pathogenesis of autoimmune diseases and developing potential therapeutic approaches.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Immunology
Jean-Charles Guery
Summary: Plasmacytoid dendritic cells (pDCs) in women produce higher levels of TLR7-driven type I interferon during HIV-1 infection, leading to stronger antiviral responses compared to men. However, excessive TLR7 response in women has been found to have a deleterious impact on acute viremia. This is attributed to specific genetic variations in TLR7 which affect protein synthesis and effector function, resulting in lower viral load during primary HIV-1 infection in females.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Grant J. Brown, Pablo F. Canete, Hao Wang, Arti Medhavy, Josiah Bones, Jonathan A. Roco, Yuke He, Yuting Qin, Jean Cappello, Julia I. Ellyard, Katharine Bassett, Qian Shen, Gaetan Burgio, Yaoyuan Zhang, Cynthia Turnbull, Xiangpeng Meng, Phil Wu, Eun Cho, Lisa A. Miosge, T. Daniel Andrews, Matt A. Field, Denis Tvorogov, Angel F. Lopez, Jeffrey J. Babon, Cristina Aparicio Lopez, Africa Gonzalez-Murillo, Daniel Clemente Garulo, Virginia Pascual, Tess Levy, Eric J. Mallack, Daniel G. Calame, Timothy Lotze, James R. Lupski, Huihua Ding, Tomalika R. Ullah, Giles D. Walters, Mark E. Koina, Matthew C. Cook, Nan Shen, Carmen de Lucas Collantes, Ben Corry, Michael P. Gantier, Vicki Athanasopoulos, Carola G. Vinuesa
Summary: Enhanced Toll-like receptor 7 (TLR7) signaling has been associated with human systemic autoimmune disease, but evidence of TLR7 gene variants causing lupus is lacking. In this study, researchers identified a newly described TLR7(Y264H) variant that increased sensing of guanosine and 2',3'-cGMP and was sufficient to cause lupus in mice. Enhanced TLR7 signaling was shown to drive aberrant B cell survival and the accumulation of specific B cell subsets, while deficiency of the downstream adapter protein MyD88 rescued autoimmunity and all phenotypes. The study highlights the importance of TLR7 and guanosine-containing self-ligands in the pathogenesis of lupus and suggests potential therapeutic targets.
Article
Medicine, Research & Experimental
Adam L. Burrack, Zoe C. Schmiechen, Michael T. Patterson, Ebony A. Miller, Ellen J. Spartz, Meagan R. Rollins, Jackson F. Raynor, Jason S. Mitchell, Tsuneyasu Kaisho, Brian T. Fife, Ingunn M. Stromnes
Summary: We investigated the differential impact of myeloid subsets on immunity to pancreatic ductal adenocarcinoma (PDA). Our study demonstrates that tumor antigenicity shapes the composition and functionality of myeloid cells. The therapeutic effectiveness of adoptive T cell therapy or programmed cell death ligand 1 blockade relies on the presence of type 1 dendritic cells (cDC1), which support the survival and function of antitumor T cells. Our research highlights the essential role of cDC1s in sustaining effective antitumor T cells and reveals differential roles for cDC1s and monocytes/macrophages in guiding T cell fate and immunotherapy response.
Article
Rheumatology
Paul C. Cremer, Antonio Abbate, Kristin Hudock, Carla McWilliams, Jinesh Mehta, Steven Y. Chang, Calvin C. Sheng, Benjamin Van Tassell, Aldo Bonaventura, Alessandra Vecchie, Brenna Carey, Qiuqing Wang, Katherine E. Wolski, Prabalini Rajendram, Abhijit Duggal, Tisha S. Wang, John F. Paolini, Bruce C. Trapnell
Summary: The trial evaluating the efficacy of mavrilimumab in COVID-19 patients with systemic hyperinflammation did not show a significant difference in the proportion of patients alive and off oxygen therapy at day 14. Larger trials are needed to further assess the potential benefits or harms of mavrilimumab therapy in this patient population.
LANCET RHEUMATOLOGY
(2021)
Article
Immunology
Cristina Bono, Paula Guerrero, Antonio Jordan-Pla, Ana Erades, Nathan Salomonis, H. Leighton Grimes, M. Luisa Gil, Alberto Yanez
Summary: Mechanistic studies are needed to uncover the details of in vivo-induced trained immunity. Using a Candida albicans live vaccine mouse model, it was found that vaccination increases trained monocytes in the bone marrow and spleen, expands and reprograms hematopoietic stem and progenitor cells (HSPCs), and mobilizes them to produce trained macrophages. Training HSPCs not only primes them for myeloid cell production but also reprograms them to produce more proinflammatory cytokines in response to a secondary challenge.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Torki Alothaimeen, Evan Trus, Sameh Basta, Katrina Gee
Summary: The study reveals differences in macrophage development and cytokine expression between M-CSF and GM-CSF cells after virus infection. While R848-induced cytokine expression is enhanced, signaling molecule activation is decreased in LCMV-infected macrophages. Interestingly, TLR7 expression is maintained at higher levels in M-CSF cells compared to GM-CSF.
JOURNAL OF GENERAL VIROLOGY
(2021)
Article
Materials Science, Biomaterials
Nina Butkovich, Jo Anne Tucker, Aaron Ramirez, Enya Li, Vijaykumar S. Meli, Edward L. Nelson, Szu-Wen Wang
Summary: The use of nanoparticles in cancer vaccines plays an important role in activating both mDCs and pDCs for antigen presentation, which has often been overlooked in the past. The study finds that nanoparticle-encapsulated TLR9 agonists activate mDCs, while both nanoparticle and individually tested constructs activate pDCs, with CpG1826 inducing pDC cytokine production. Furthermore, factors secreted from pDCs stimulated with a vaccine formulation comprising peptide antigen and CpG1826 enhance mDC antigen display.
BIOMATERIALS SCIENCE
(2023)
Article
Immunology
Seul Hye Ryu, Hyun Soo Shin, Hye Hyeon Eum, Ji Soo Park, Wanho Choi, Hye Young Na, Hyunju In, Tae-Gyun Kim, Sejung Park, Soomin Hwang, Moah Sohn, Eun-Do Kim, Kyoung Yul Seo, Hae-Ock Lee, Min-Geol Lee, Min Kyung Chu, Chae Gyu Park
Summary: Dendritic cells (DCs) play a crucial role in initiating adaptive immunity. Injecting granulocyte macrophage-colony stimulating factor (GM-CSF) can generate a distinct subset of DCs called GMiDCs in the spleen, which have superior antigen-presenting ability and can activate CD4(+) T cells and polarize Th2 cells. The generation of GMiDCs depends on the expression of GM-CSF receptor, and they can also be found in other tissues and during chronic allergic inflammation. This study provides important insights into the development and immune regulation of DCs.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Guoliang Cao, Mixiao Tan, Wenping Huang, Jie Zhang, Yue Yin, Xiaoyang Li, Haixia Ma, Wen Su, Suping Li, Haitao Ran, Shih-Hsin Ho, Hai Wang
Summary: Efforts to improve the delivery efficiency of nanocarriers in tumor sites have mainly focused on materials rather than the biological system. However, we have shown that short-term starvation (STS) can enhance the cellular uptake and tumor accumulation of nanocarriers. By utilizing necrotic cell debris vesicles (NCDVs) and developing a NCDVs-mimicking nanoprotoplast, we were able to efficiently deliver inhibitors and trigger an immune response, further augmented by STS treatment.
Article
Immunology
Takaki Asano, Bertrand Boisson, Fanny Onodi, Daniela Matuozzo, Marcela Moncada-Velez, Majistor Raj Luxman Maglorius Renkilaraj, Peng Zhang, Laurent Meertens, Alexandre Bolze, Marie Materna, Sarantis Korniotis, Adrian Gervais, Estelle Talouarn, Benedetta Bigio, Yoann Seeleuthner, Kaya Bilguvar, Yu Zhang, Anna-Lena Neehus, Masato Ogishi, Simon J. Pelham, Tom Le Voyer, Jeremie Rosain, Quentin Philippot, Pere Soler-Palacin, Roger Colobran, Andrea Martin-Nalda, Jacques G. Riviere, Yacine Tandjaoui-Lambiotte, Khalil Chaibi, Mohammad Shahrooei, Ilad Alavi Darazam, Nasrin Alipour Olyaei, Davood Mansouri, Figen Palabiyik, Tayfun Ozcelik, Giuseppe Novelli, Antonio Novelli, Giorgio Casari, Alessandro Aiuti, Paola Carrera, Simone Bondesan, Federica Barzaghi, Patrizia Rovere-Querini, Cristina Tresoldi, Jose Luis Franco, Julian Rojas, Luis Felipe Reyes, Ingrid G. Bustos, Andres Augusto Arias, Guillaume Morelle, Christele Kyheng, Jesus Troya, Laura Planas-Serra, Agatha Schluter, Marta Gut, Aurora Pujol, Luis M. Allende, Carlos Rodriguez-Gallego, Carlos Flores, Oscar Cabrera-Marante, Daniel E. Pleguezuelo, Rebeca Perez de Diego, Sevgi Keles, Gokhan Aytekin, Ozge Metin Akcan, Yenan T. Bryceson, Peter Bergman, Petter Brodin, Daniel Smole, C. I. Edvard Smith, Anna-Carin Norlin, Tessa M. Campbell, Laura E. Covill, Lennart Hammarstrom, Qiang Pan-Hammarstrom, Hassan Abolhassani, Shrikant Mane, Nico Marr, Manar Ata, Fatima Al Ali, Taushif Khan, Andras N. Spaan, Clifton L. Dalgard, Paolo Bonfanti, Andrea Biondi, Sarah Tubiana, Charles Burdet, Robert Nussbaum, Amanda Kahn-Kirby, Andrew L. Snow, Jacinta Bustamante, Anne Puel, Stephanie Boisson-Dupuis, Shen-Ying Zhang, Vivien Beziat, Richard P. Lifton, Paul Bastard, Luigi D. Notarangelo, Laurent Abel, Helen C. Su, Emmanuelle Jouanguy, Ali Amara, Vassili Soumelis, Aurelie Cobat, Qian Zhang, Jean-Laurent Casanova
Summary: A study revealed that rare X-linked TLR7 variants were found in 16 unrelated male individuals with critical COVID-19 pneumonia out of a cohort of 1202 male patients. No such variants were detected in 331 asymptomatic or mildly infected male individuals.
SCIENCE IMMUNOLOGY
(2021)
Article
Immunology
Yawen Wang, Annie Roussel-Queval, Lionel Chasson, Noel Hanna Kazazian, Laetitia Marcadet, Andrianos Nezos, Michael H. Sieweke, Clio Mavragani, Lena Alexopoulou
Summary: The study reveals that TLR7 signaling plays a crucial role in the development of Sjogren's syndrome (SS), and the SS phenotype in TLR8-deficient mice is primarily dependent on TLR7. Inhibiting TLR7 signaling may have therapeutic value for the treatment of SS.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Gastroenterology & Hepatology
Wei Si, Hua Liang, Jason Bugno, Qi Xu, Xingchen Ding, Kaiting Yang, Yanbin Fu, Ralph R. Weichselbaum, Xin Zhao, Liangliang Wang
Summary: Our study demonstrates that oral administration of live LGG enhances the antitumour activity of anti-PD-1 immunotherapy by increasing tumour-infiltrating DCs and T cells, and by shifting the gut microbial community towards enrichment in Lactobacillus murinus and Bacteroides uniformis. Mechanistically, LGG induces IFN-beta production via the cGAS/STING/TBK1/IRF7 axis in DCs, highlighting the potential of LGG as a combination agent with ICB for cancer therapies.
Article
Medicine, Research & Experimental
Haylee A. Cosgrove, Sebastien Gingras, Minjung Kim, Sheldon Bastacky, Jeremy S. Tilstra, Mark J. Shlomchik
Summary: The regulatory role of Toll-like receptor 7 (TLR7) in the pathogenesis of lupus and its potential therapeutic strategy have been studied. The study found that TLR7 deficiency in B cells greatly improved the disease progression in TLR9-deficient mice with accelerated systemic lupus erythematosus, suggesting the importance of B cell-directed TLR7 regulation in lupus.
Article
Immunology
Paoline Laurent, Chao Yang, Andre F. Rendeiro, Benjamin E. Nilsson-Payant, Lucia Carrau, Vasuretha Chandar, Yaron Bram, Benjamin R. tenOever, Olivier Elemento, Lionel B. Ivashkiv, Robert E. Schwartz, Franck J. Barrat
Summary: Lung-infiltrating macrophages play a role in the inflammation and lethality of COVID-19, despite not being infected by the virus. Plasmacytoid dendritic cells (pDCs) in the lungs produce interferons (IFN-I) in response to SARS-CoV-2, leading to macrophage activation. This interaction between pDCs and macrophages may contribute to the severity of the disease.
SCIENCE IMMUNOLOGY
(2022)
Article
Genetics & Heredity
Giulia Leoni, Marguerite Y. Wasowicz, Mario Chan, Cuixiang Meng, Raymond Farley, Steven L. Brody, Makoto Inoue, Mamoru Hasegawa, Eric W. F. W. Alton, Uta Griesenbach
CURRENT GENE THERAPY
(2015)
Article
Biochemistry & Molecular Biology
N. Tanaka, K. Araki, D. Mizokami, Y. Miyagawa, T. Yamashita, M. Tomifuji, Y. Ueda, M. Inoue, K. Matsushita, F. Nomura, H. Shimada, A. Shiotani
Article
Biochemistry & Molecular Biology
T. Kurioka, K. Mizutari, K. Niwa, T. Fukumori, M. Inoue, M. Hasegawa, A. Shiotani
Article
Immunology
Julien Nyombayire, Omu Anzala, Brian Gazzard, Etienne Karita, Philip Bergin, Peter Hayes, Jakub Kopycinski, Gloria Omosa-Manyonyi, Akil Jackson, Jean Bizimana, Bashir Farah, Eddy Sayeed, Christopher L. Parks, Makoto Inoue, Takashi Hironaka, Hiroto Hara, Tsugumine Shu, Tetsuro Matano, Len Dally, Burc Barin, Harriet Park, Jill Gilmour, Angela Lombardo, Jean-Louis Excler, Patricia Fast, Dagna S. Laufer, Josephine H. Cox
JOURNAL OF INFECTIOUS DISEASES
(2017)
Article
Respiratory System
Eric W. F. W. Alton, Jeffery M. Beekman, A. Christopher Boyd, June Brand, Marianne S. Carlon, Mary M. Connolly, Mario Chan, Sinead Conlon, Heather E. Davidson, Jane C. Davies, Lee A. Davies, Johanna F. Dekkers, Ann Doherty, Sabrina Gea-Sorli, Deborah R. Gill, Uta Griesenbach, Mamoru Hasegawa, Tracy E. Higgins, Takashi Hironaka, Laura Hyndman, Gerry McLachlan, Makoto Inoue, Stephen C. Hyde, J. Alastair Innes, Toby M. Maher, Caroline Moran, Cuixiang Meng, Michael C. Paul-Smith, Ian A. Pringle, Kamila M. Pytel, Andrea Rodriguez-Martinez, Alexander C. Schmidt, Barbara J. Stevenson, Stephanie G. Sumner-Jones, Richard Toshner, Shu Tsugumine, Marguerite W. Wasowicz, Jie Zhu
Article
Medicine, Research & Experimental
Kazuya Goto, Keiko Imamura, Kenichi Komatsu, Kohnosuke Mitani, Kazuhiro Aiba, Norio Nakatsuji, Makoto Inoue, Akihiro Kawata, Hirofumi Yamashita, Ryosuke Takahashi, Haruhisa Inoue
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2017)
Article
Cell & Tissue Engineering
Kazutaka Miyamoto, Mizuha Akiyama, Fumiya Tamura, Mari Isomi, Hiroyuki Yamakawa, Taketaro Sadahiro, Naoto Muraoka, Hidenori Kojima, Sho Haginiwa, Shota Kurotsu, Hidenori Tani, Li Wang, Li Qian, Makoto Inoue, Yoshinori Ide, Junko Kurokawa, Tsunehisa Yamamoto, Tomohisa Seki, Ryo Aeba, Hiroyuki Yamagishi, Keiichi Fukuda, Masaki Ieda
Article
Biochemistry & Molecular Biology
Michael C. Paul-Smith, Kamila M. Pytel, Jean-Francois Gelinas, Jenny McIntosh, Ian Pringle, Lee Davies, Mario Chan, Cuixiang Meng, Robyn Bell, Lidia Cammack, Caroline Moran, Loren Cameron, Makoto Inoue, Shu Tsugumine, Takashi Hironaka, Deborah R. Gill, Stephen C. Hyde, Amit Nathwani, Eric W. F. W. Alton, Uta Griesenbach
Article
Hematology
Y. Kashiwakura, T. Ohmori, J. Mimuro, S. Madoiwa, M. Inoue, M. Hasegawa, K. Ozawa, Y. Sakata
Article
Virology
Nami Iwamoto, Naofumi Takahashi, Sayuri Seki, Takushi Nomura, Hiroyuki Yamamoto, Makoto Inoue, Tsugumine Shu, Taeko K. Naruse, Akinori Kimura, Tetsuro Matano
JOURNAL OF VIROLOGY
(2014)
Article
Immunology
Mao Isshiki, Xianfeng Zhang, Hirotaka Sato, Takashi Ohashi, Makoto Inoue, Hisatoshi Shida
Article
Gastroenterology & Hepatology
Kazuyuki Matsushita, Hideaki Shimada, Yasuji Ueda, Makoto Inoue, Mamoru Hasegawa, Takeshi Tomonaga, Hisahiro Matsubara, Fumio Nomura
WORLD JOURNAL OF GASTROENTEROLOGY
(2014)
Article
Otorhinolaryngology
Yuya Tanaka, Koji Araki, Shingo Tanaka, Yoshihiro Miyagawa, Hiroshi Suzuki, Daisuke Kamide, Masayuki Tomifuji, Kosuke Uno, Eiko Harada, Taku Yamashita, Yasuji Ueda, Makoto Inoue, Akihiro Shiotani
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
(2019)
Article
Immunology
Hiroki Takeuchi, Keiko Imamura, Bin Ji, Kayoko Tsukita, Takako Enami, Keizo Takao, Tsuyoshi Miyakawa, Masato Hasegawa, Naruhiko Sahara, Nobuhisa Iwata, Makoto Inoue, Hideo Hara, Takeshi Tabira, Maiko Ono, John Q. Trojanowski, Virginia M. -Y. Lee, Ryosuke Takahashi, Tetsuya Suhara, Makoto Higuchi, Haruhisa Inoue
Article
Surgery
Tsuyoshi Takamura, Hiroshi Sasaki, Haruyuki Hirayama, Akihiko Kiyoshi, Makoto Inoue, Kenji Matsui, Naoto Matsumoto, Yatsumu Saito, Toshinari Fujimoto, Susumu Tajiri, Shuichiro Yamanaka, Kei Matsumoto, Takeshi Miyawaki, Takashi Yokoo, Eiji Kobayashi
Summary: This study improved the classical xenotransplantation model by transplanting porcine kidneys into size-matched orthotopic positions. The surgical details and results showed that this method contributes to stable blood flow to the kidneys post-transplantation.
ACTA CIRURGICA BRASILEIRA
(2021)