Article
Biotechnology & Applied Microbiology
Ling Yang, Kunlin Wu, Shengxiang Qiu, Honglin Cao, Shulin Deng
Summary: This study successfully established a regeneration system for the multifunctional shrub Rhodomyrtus tomentosa by inducing callus from tender leaves and stems. The induced callus was light yellow-green and rigid, with granules, and 93.33% of it differentiated into adventitious shoots within 60 days. The optimal shoot proliferation medium was 1/2 MS medium with 1 mg L-1 6-BA and 0.1 mg L-1 NAA, and the highest regeneration response rate (86.67%) was obtained under high temperature and high light intensity conditions (26 degrees C, 120 mu mol m(-2)s(-1)). Adventitious roots showed the highest rate of rooting (100%) on PGR-free 1/2 MS medium. Over 90% of plantlets survived when transferred to a substrate of sand/soil (1:2 ratio) within 2 months.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Multidisciplinary Sciences
Ajmal Khan, Azhar Hussain Shah, Niaz Ali
Summary: Efficient protocols for callus induction and micropropagation of Saussurea costus were developed, and the phytochemical diversity of wild and in-vitro propagated material was investigated. The study revealed variations in biochemical contents in callus cultured on different stress media, with the highest flavonoids and anthocyanin contents observed in in-vitro propagated plants. Additionally, GC-MS analysis of the callus ethyl acetate extracts revealed 24 different phytochemicals, showing variability in secondary metabolites of both wild and propagated plants/callus.
SCIENTIFIC REPORTS
(2021)
Article
Horticulture
Ali Mohsen Al-Ali, Yaser Hassan Dewir, Rashid Sultan Al-Obeed
Summary: This study presents an effective method for axillary shoot proliferation in Al-Taif rose, an important cultivar for rose oil industry. By optimizing the culture conditions, including the addition of cytokinins, light/dark incubation, and culture type, the rate of shoot proliferation and growth can be significantly improved. Liquid culture using bioreactors proves to be more suitable for rose propagation than gelled culture.
Review
Biotechnology & Applied Microbiology
Barbara Thiem, Anastasia Aliesa Hermosaningtyas, Anna Budzianowska, Malgorzata Kikowska
Summary: This article summarises the development of an in vitro propagation method for selected medicinal plants by Polish researchers and botanists. The utilization of medicinal plants as a source of raw materials for the pharmaceutical industry has gained significant attention since the 1980s. Medicinal plants have been identified as a potential alternative source for essential compounds with various applications. The plant micropropagation system provides a solution to both the uniform biomass production for bioactive secondary metabolites and the conservation of rare or endangered plant species.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Biology
Leila Abdi Saray, Mohammad Ahmadabadi, Rana Valizadeh Kamran
Summary: In this study, reliable protocols for in vitro shoot regeneration of Tanacetum parthenium L. Schultz-Bip. were established using leaf and internode explants. The highest regeneration rate was achieved using a combination of BAP cytokinin and NAA auxin, and the regenerated plants were proven to be suitable for gene transfer experiments in molecular breeding programs.
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
(2022)
Article
Plant Sciences
Mohammed Alsafran, Kokila Wickramanayake, Kamal Usman, Talaat Ahmed
Summary: In vitro plant cell and tissue cultures are powerful tools in conserving and managing plant genetic resources. A reliable micropropagation protocol was developed for efficient callus proliferation and shoot regeneration of Meseika plants. This protocol allows for rapid in-vitro multiplication of Meseika shoots and callus.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Md. Sanower Hossain, Zannat Urbi, Ing Chia Phang
Summary: The study found that using 3.0mM IBA can more efficiently induce stem growth in young apical shoot cuttings of Andrographis paniculata, with young apical shoot cuttings being more effective compared to old apical shoot cuttings. This suggests that mass propagation of Andrographis paniculata can be achieved in a short time using IBA.
Article
Agricultural Engineering
Annalisa Cartabia, Virginia Sarropoulou, Katerina Grigoriadou, Eleni Maloupa, Stephane Declerck
Summary: A protocol for the in vitro production of Alkanna tinctoria plants using shoot-tip explants was developed, which improved proliferation, rooting, and acclimatization of the micropropagated shoot-tip explants. This protocol opens the door to industrial exploitation of the plant's secondary metabolites and the conservation of this important medicinal plants.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Review
Agronomy
Min-Rui Wang, Wen-Lu Bi, Jean Carlos Bettoni, Dong Zhang, Gayle M. Volk, Qiao-Chun Wang
Summary: Diseases caused by plant pathogens lead to significant economic losses and restrictions on the movement of plant materials. Using pathogen-free planting materials and shoot tip cryotherapy can effectively manage these diseases and facilitate global exchange of genetic resources.
Article
Plant Sciences
Lilyana Nacheva, Nataliya Dimitrova, Lyubka Koleva-Valkova, Miroslava Stefanova, Tsveta Ganeva, Marieta Nesheva, Ivan Tarakanov, Andon Vassilev
Summary: This study investigated the effects of LED light sources on the growth and rooting of plum rootstock Saint Julien. The results showed that mixed light (WBR) was the best choice during the multiplication phase, while red LED light was more suitable during the rooting stage.
Article
Biology
Merve Sifa Hane Kose, Muhammet Dogan, Gokhan Sadi
Summary: This study aimed to achieve multiple and rapid propagations of Staurogyne repens using tissue culture techniques. The shoot regeneration capabilities of the nodal explants were high due to the growth regulators used, with shoot regeneration frequencies ranging between 77.77% and 100.00% in different applications of BAP-IAA and KIN-IAA.
Article
Multidisciplinary Sciences
Jyotsna Sharma, Anuja Koul, Savita Sharma, Raju Shankarayan, Sharada Mallubhotla
Summary: Efficient micropropagation protocol for Nanorrhinum ramosissimum was developed, utilizing shoot tip explants inoculated on half-strength MS medium supplemented with KN and IAA. Shoot organogenesis was enhanced with dextrose in multiplication medium, and optimal rooting frequency was achieved on medium containing only IAA. In vitro plantlets acclimatized successfully with 95% survival under greenhouse conditions, demonstrating the potential of this protocol for germplasm conservation of this medicinal plant.
KUWAIT JOURNAL OF SCIENCE
(2021)
Article
Agronomy
Aikaterini N. Martini, Georgia Vlachou, Maria Papafotiou
Summary: This study investigated the micropropagation of Salvia tomentosa, focusing on factors such as explant origin and plant growth regulators in the culture medium, to optimize the establishment, shoot proliferation, and rooting in vitro. By controlling these factors, the micropropagation protocol developed can contribute to the promotion of S. tomentosa in the floriculture and pharmaceutical industry, as well as breeding programs targeting high value products.
Article
Horticulture
Yeganeh Basiri, Nematollah Etemadi, Mahdi Alizadeh, Javad Alizargar
Summary: In this study, an in vitro propagation technique for Eremurus spectabilis M. Bieb using tuberous root explants was established. The highest callus induction and shoot proliferation rates were observed on specific media compositions. The protocol can have commercial applications and be useful in the production of secondary metabolites.
Review
Plant Sciences
Guifang Zhang, Wu Liu, Zewei Gu, Shasha Wu, E. Yilan, Wenkun Zhou, Jinxing Lin, Lin Xu
Summary: This review summarizes the important role of jasmonate in plant regeneration. Jasmonate acts as a hormone linking wounding to different types of regeneration by regulating stem cell activities, cell proliferation, cell fate transition, and auxin production.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Wei-chi Ku, Yi-ling Chang, Sheng-fa Wu, Hui-nung Shih, Yew-min Tzeng, Hui-ru Kuo, Kai-ming Chang, Dinesh Chandra Agrawal, Bing-lan Liu, Chin-an Chang, Siendong Huang, Meng-jen Lee
Article
Plant Sciences
Huang-Chi Wang, Hsin-Sheng Tsay, Hui-Nung Shih, Yi-An Chen, Kai-Ming Chang, Dinesh Chandra Agrawal, Siendong Huang, Yi-Lo Lin, Meng-Jen Lee
PHARMACEUTICAL BIOLOGY
(2018)
Proceedings Paper
Multidisciplinary Sciences
Tao-Ming Cheng, Hsing-Yu Hou, Sung-Chi Hsu, Dinesh Chandra Agrawal, Rung-Ching Chen, Mei-Ling Liu
ADVANCED SCIENCE LETTERS
(2018)
Article
Chemistry, Multidisciplinary
Chin-Tung Wu, Dinesh Chandra Agrawal, Wen-Ying Huang, Hsiu-Cheng Hsu, Shang-Jhen Yang, Shu-Ling Huang, Yung-Sheng Lin
JOURNAL OF CHEMISTRY
(2019)
Article
Biotechnology & Applied Microbiology
Wen-Che Tsai, Hung-Chi Chang, Yi-Han Tseng, Hsin-Yi Yin, Jiunn-Wang Liao, Dinesh Chandra Agrawal, Hsiao-Wei Wen
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
(2020)
Article
Plant Sciences
Hung-Chi Chang, Hui-Min Xie, Maw-Rong Lee, Chiu-Ying Lin, Mei-Kuen Yip, Dinesh Chandra Agrawal, Hsin-Sheng Tsay
Article
Biotechnology & Applied Microbiology
Wen-Che Tsai, Hung-Chi Chang, Hsin-Yi Yin, Meng-Chieh Huang, Dinesh Chandra Agrawal, Hsiao-Wei Wen
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
(2020)
Article
Plant Sciences
Chia-Chen Chen, Maw-Rong Lee, Chi-Rei Wu, Hsin-Ju Ke, Hui-Min Xie, Hsin-Sheng Tsay, Dinesh Chandra Agrawal, Hung-Chi Chang
Article
Food Science & Technology
Sahri Yanti, Dinesh Chandra Agrawal, Dinar Suksmayu Saputri, Hung-Yu Lin, Wei-Jyun Chien
Summary: This study aims to analyze the nutrient levels in different edible seeds and nuts, including Sacha inchi residue, and predict the five macronutrients using NIR spectroscopy. Results showed that candlenut is rich in oil, while Sacha inchi residue is rich in minerals, protein, and moisture, providing valuable information for consumers and nutrition professionals.
INTERNATIONAL JOURNAL OF FOOD SCIENCE
(2022)
Article
Chemistry, Medicinal
Yi-Lo Lin, Jiunn-Wang Liao, Shunching Wang, Badrinathan Sridharan, Hsin-Ju Lee, Ai Li, Kai-Ming Chang, Ching-Yang Wu, Siendong Huang, Kai-Ting Chang, Dinesh Chandra Agrawal, Ching-Jung Chen, Meng-Jen Lee
Summary: In this study, it was found that Andrographolide, a compound derived from Andrographis paniculata, can reduce pain sensation in a rat post-operative wound model. The results showed that Andrographolide effectively decreased mechanical allodynia and influenced the expression of certain proteins related to pain and wound healing.
Article
Multidisciplinary Sciences
Sahri Yanti, Wei-Jyun Chien, Dinesh Chandra Agrawal
Summary: The study confirms that resveratrol can stabilize insulin structure and prevent insulin resistance in diabetes through its interaction with insulin.
BENI-SUEF UNIVERSITY JOURNAL OF BASIC AND APPLIED SCIENCES
(2022)
Article
Chemistry, Analytical
Siti Raudhatul Kamali, Chang-Nan Chen, Dinesh Chandra Agrawal, Tai-Huei Wei
Summary: This study synthesized a high sensitive fluorescent probe based on sulfur-doped carbon dots using microwave irradiation method, which showed strong blue emission and high quantum yield. The probe exhibited good sensitivity for Cr(III) detection with high recoveries in natural water samples.
JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Sahri Yanti, Zhong-Wen Wu, Dinesh Chandra Agrawal, Wei-Jyun Chien
Summary: The study revealed that phloretin (PHL) has a significant interaction with insulin (INS), forming hydrogen bonds and hydrophobic interactions. Temperature changes affect the interaction, causing a redshift in the emission wavelength of 305 to 317 nm. The thermodynamic parameters show that the interaction between PHL and INS is spontaneous, with enthalpy (Delta H-0) and entropy (Delta S-0) values of -16,514 kJ/mol and 22.65 J/mol center dot K, respectively.
JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY
(2021)
Article
Education & Educational Research
Dinesh Chandra Agrawal, Hsing-Yu Hou, Tao-Ming Cheng
Summary: Teaching evaluation is essential in higher education, with alumni feedback being equally important. This study analyzed data from the 'University Career and Competency Assessment Network', showing that 'Learn-Practice Fit' positively influenced workplace 'Satisfaction', and 'Responsibility and discipline' had significant positive correlations with 'Communication and Expression', 'Interpersonal Interaction', and 'Teamwork'.
INTERNATIONAL JOURNAL OF INFORMATION AND COMMUNICATION TECHNOLOGY EDUCATION
(2021)
Article
Education & Educational Research
Tao-Ming Cheng, Hsing-Yu Hou, Dinesh Chandra Agrawal, Ching-Fang Liaw, Rung-Ching Chen
ASIA PACIFIC JOURNAL OF EDUCATION
(2018)