Review
Cell Biology
Jana Janockova, Lucia Slovinska, Denisa Harvanova, Timea Spakova, Jan Rosocha
Summary: Mesenchymal stem cells (MSCs) are shown to have great potential in treating diseases through the secretion of exosomes, which are believed to be more effective than the cells themselves. Alternative approaches using MSC-derived exosomes offer promise in overcoming limitations in cell-based therapy. MSC-derived exosomes have significant therapeutic potential and are promising in regenerative medicine.
JOURNAL OF BIOMEDICAL SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Riccardo Giorgino, Domenico Albano, Stefano Fusco, Giuseppe M. Peretti, Laura Mangiavini, Carmelo Messina
Summary: Osteoarthritis (OA) is a common chronic orthopedic disorder that primarily affects hips and knees. The variability in the prevalence of knee OA across studies highlights the heterogeneity of reported data worldwide. The pathogenetic mechanism of knee OA involves an inflammatory process in the synovial membrane, mediated by immune system activation and damage-associated molecular patterns (DAMPs). Mesenchymal stem cells (MSCs) offer promising therapeutic options, but unresolved issues remain regarding the optimal cell source and quantity. Further research is necessary to address these uncertainties. This review provides a comprehensive overview of the molecular mechanisms of cartilage damage in knee OA and discusses current regenerative medicine approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Mozafar Khazaei, Fatemeh Khazaei, Elham Niromand, Elham Ghanbari
Summary: Tissue engineering offers a promising approach for improving the treatment of type 1 diabetes by utilizing mesenchymal stem cells (MSCs) to differentiate into insulin-producing cells (IPCs). The use of scaffolds can enhance the differentiation of MSCs into IPCs, improving their viability and insulin production.
JOURNAL OF DRUG TARGETING
(2023)
Review
Medicine, Research & Experimental
Jianghong Huang, Qisong Liu, Jiang Xia, Xi Chen, Jianyi Xiong, Lei Yang, Yujie Liang
Summary: This article comprehensively reviews the homing effect of stem cells in joints and highlights progress towards the targeted modification of MSCs.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Lidia Ibanez, Paloma Guillem-Llobat, Marta Marin, Maria Isabel Guillen
Summary: Mesenchymal stem cells have shown promising effects in the treatment of osteoarthritis, but there is a lack of research focusing on their effects on osteoclasts. Further studies are needed to support the use of mesenchymal stem cells as a therapeutic tool.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Michal Domaniza, Marian Hluchy, Dasa Cizkova, Filip Humenik, Lucia Slovinska, Nikola Hudakova, Lubica Hornakova, Juraj Vozar, Alexandra Trbolova
Summary: This study aimed to investigate the potential of cell-based regenerative therapy for elbow joints affected by osteoarthritis. The results showed that two injections of amnion-derived mesenchymal stem cells improved joint range of motion and had an impact on synovial fluid biomarkers. Concentrations of IFN-γ, IL-6, and TNF-α decreased while IL-10 increased between day 0 and day 28 after treatment.
Article
Engineering, Biomedical
Lang Bai, Qibin Han, Zeyu Han, Xiaoyu Zhang, Jingwen Zhao, Huitong Ruan, Junliang Wang, Feng Lin, Wenguo Cui, Xing Yang, Yuefeng Hao
Summary: By utilizing chitosan-based cell adhesion, a bioadhesive porous microsphere is developed as a cell carrier for accelerating articular cartilage regeneration. After injection into the joint cavity, the microspheres expedite the release of mesenchymal stem cells (MSCs) in the microenvironment of arthritis inflammation, while using kartogenin to promote the differentiation of MSCs into chondrocytes, thereby accelerating articular cartilage regeneration.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Cell & Tissue Engineering
Oldouz Shareghi-oskoue, Leili Aghebati-Maleki, Mehdi Yousefi
Summary: Premature ovarian failure (POF) is a problem for women before the age of 40, characterized by symptoms such as amenorrhea and low estrogen levels. The current treatment approach involves stem cell therapy, particularly HUCMSC transplantation, which has shown to effectively improve ovarian function and treat infertility.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Biochemistry & Molecular Biology
Sree Samanvitha Kuppa, Hyung Keun Kim, Ju Yeon Kang, Seok Cheol Lee, Jong Keun Seon
Summary: Osteoarthritis is a low-grade inflammatory disorder of the joints, but there are limited treatment options currently available. This article explores the use of mesenchymal stem cells and their secreted factors for intervention in osteoarthritis, with a focus on the polarization of macrophages to reduce inflammation and symptoms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Ilias Nikolits, Sabrina Nebel, Dominik Egger, Sebastian Kress, Cornelia Kasper
Summary: Mesenchymal stem cells (MSCs) are valuable for cell-based therapies due to their differentiation and immunomodulatory capabilities, but prolonged in vitro culture may diminish these properties. Studies have focused on optimizing culture protocols to preserve MSC phenotype and functionality during early expansion phases.
Article
Cell & Tissue Engineering
Yugo Miura, Kentaro Endo, Keiichiro Komori, Ichiro Sekiya
Summary: This study aimed to improve the biological function of osteoarthritis (OA) synovial mesenchymal stem cells (MSCs) by removing senescent cells using the senolytic drug ABT-263. The results showed that ABT-263 pretreatment significantly reduced the percentage of senescent cells and increased apoptosis in MSCs. The pretreated cells also showed enhanced colony formation ability and adipogenic and chondrogenic capabilities, as well as lower expression of senescence markers and inflammation factors.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Immunology
Pan Luo, Qiling Yuan, Xianjie Wan, Mingyi Yang, Peng Xu
Summary: The pathological manifestations of osteoarthritis (OA) involve destruction of articular cartilage, synovial sac thickening, subchondral osteosclerosis, and osteophyte formation. Various cell types, including chondrocytes, osteoblasts, osteoclasts, synovial fibroblasts, T cells, macrophages, and mesenchymal stem cells (MSCs), are involved in OA. Immune cells, especially dendritic cells and macrophages, play significant roles in the pathogenesis of OA. Additionally, adaptive immune cells such as T-cell subsets, B-cell subsets, and NK cells are important in OA pathogenesis. MSCs not only play a key role in maintaining the dynamic balance and repairing OA tissue but also have immunomodulatory properties. Therefore, MSCs have potential as a therapeutic agent for OA. This review summarizes the effects of immune cells and cytokines on different cells in OA, aiming to identify new therapeutic targets based on the immune system and cytokine activity sites to prevent OA development.
JOURNAL OF INFLAMMATION RESEARCH
(2023)
Article
Immunology
Song Peng, Chang Sun, Chengteng Lai, Lei Zhang
Summary: The study evaluated the potential pro-inflammatory effects and molecular mechanism of MSC-Exos in Osteoarthritis. MSC-Exos improved osteoarthritis by reducing inflammation levels, preventing ferroptosis, and inducing expression of GOT1/CCR2 to regulate ferroptosis. In addition, MSC-Exos promoted cell growth and osteogenic differentiation in an in vitro model.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Review
Cell Biology
Candice Bohaud, Rafael Contreras-Lopez, Jholy de la Cruz, Claudia Terraza-Aguirre, Mingxing Wei, Farida Djouad, Christian Jorgensen
Summary: Osteoarthritis is a multifaceted degenerative and inflammatory joint disorder, with current therapies often only providing short-term benefits without complete structural and functional reconstitution. Identifying novel therapeutic targets and strategies is crucial to achieve long-term clinical benefits for OA patients.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Ali Hassanzadeh, Nasim Vousooghi, Ramin Rahimnia, Ehsan Razeghian, Simin Rajaeian, Iman Seyhoun, Shiva Sharif, Javad Verdi
Summary: Recently, transplantation of mesenchymal stem/stromal cells (MSCs) has emerged as a promising treatment option for osteoarthritis (OA) patients, showing potential benefits in supporting cartilage structure and improving knee function. MSCs exert their effects by inhibiting inflammation and immunomodulation through the release of anti-inflammatory mediators. Additionally, they promote chondrocyte proliferation, maintain extracellular matrix homeostasis, and suppress matrix metalloproteinases activities, ultimately providing chondroprotection. Numerous studies have demonstrated that MSCs therapy can significantly reduce pain and restore knee function in OA patients. This review focuses on recent advances in MSCs-based therapeutics for inducing chondrogenic and chondroprotective effects in OA patients, with a specific emphasis on in vivo results from the past decade.
CELL BIOLOGY INTERNATIONAL
(2023)
Article
Cell & Tissue Engineering
Kiran Shah, Tara Drury, Ivelise Roic, Peter Hansen, Mark Malin, Richard Boyd, Huseyin Sumer, Ray Ferguson
STEM CELLS INTERNATIONAL
(2018)
Article
Cell & Tissue Engineering
Julien Freitag, Kiran Shah, James Wickham, Douglas Li, Cameron Norsworthy, Abi Tenen
REGENERATIVE MEDICINE
(2020)
Article
Cell & Tissue Engineering
Julien Freitag, James Wickham, Kiran Shah, Douglas Li, Cameron Norsworthy, Abi Tenen
REGENERATIVE MEDICINE
(2020)
Review
Cell & Tissue Engineering
Ashley G. Zhao, Kiran Shah, Brett Cromer, Huseyin Sumer
STEM CELLS INTERNATIONAL
(2020)
Article
Cell & Tissue Engineering
Ashley G. Zhao, Kiran Shah, Julien Freitag, Brett Cromer, Huseyin Sumer
STEM CELLS INTERNATIONAL
(2020)
Article
Oncology
Ashley G. Zhao, Kiran Shah, Brett Cromer, Huseyin Sumer
Summary: This study optimized the isolation method for EVs derived from MSCs and found that 1 hour of differential ultracentrifugation is the optimal method for isolating high-quality and quantity of MSC-derived EVs.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Cell & Tissue Engineering
Julien Freitag, James Wickham, Kiran Shah, Abi Tenen
Summary: This study assessed the effectiveness of stem cell therapy in the treatment of knee osteoarthritis. The results showed that stem cell therapy is an effective, safe, and long-lasting treatment option, significantly reducing knee pain and improving function. It may also delay the need for knee replacement surgery, providing global health and economic benefits.
REGENERATIVE MEDICINE
(2022)
Review
Cell & Tissue Engineering
Kiran Shah, Nirali Shah, Fatameh Ghassemi, Carolyn Ly, Teena George, Carla Lutz, Huseyin Sumer
Summary: Cellular therapies, particularly allogeneic MSCs, have been approved and recognized for their potential in treating various diseases, with ongoing clinical trials showing promising results. It is important to address safety concerns and efficacy issues in these emerging therapies to ensure their successful implementation.
STEM CELLS INTERNATIONAL
(2022)
