☆ 4.3 Correction

Reliable transgene-independent method for determining sleeping beauty transposon copy numbers (vol 2, pg 5, 2011)

MOBILE DNA (2013)

Journal

MOBILE DNA

Volume 4, Issue -, Pages -

Publisher

BIOMED CENTRAL LTD

DOI: 10.1186/1759-8753-4-11

Keywords

-

Categories

Genetics & Heredity

Ask authors/readers for more resources

Protocol

Community support

Reagent

Community support

Authors

I am an author on this paper

Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.3

Not enough ratings

Secondary Ratings

Novelty

-

Significance

-

Scientific rigor

-

Rate this paper

Recommended

Article Biochemistry & Molecular Biology

Engineered Sleeping Beauty Transposon as Efficient System to Optimize Chimp Adenoviral Production

Samantha Baldassarri, Daniela Benati, Federica D'Alessio, Clarissa Patrizi, Eleonora Cattin, Michela Gentile, Angelo Raggioli, Alessandra Recchia

Summary: This study demonstrates the use of SB transposon to engineer HEK293 cells for improved production of chimpanzee Adenovector. By employing genetic engineering and CRISPR/Cas9 technology, the essential genes for chimpanzee Adenoviral DNA replication were successfully integrated into HEK293 cells, leading to significantly enhanced vector production.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

Add to Collection

Article Biochemistry & Molecular Biology

Transgene Expression and Transposition Efficiency of Two-Component Sleeping Beauty Transposon Vector Systems Utilizing Plasmid or mRNA Encoding the Transposase

Natalie Tschorn, Yasemin van Heuvel, Jorn Stitz

Summary: The use of two-component transposon plasmid vector systems can expedite the development of recombinant cell lines, but the undesired stable transfection and sustained expression of the transposase construct can lead to genetic instability. In this study, we established three recombinant cell pools using a Sleeping Beauty-derived vector system and demonstrated stable integration and sustained expression of the transposase for 48 days. To provide an alternative approach, we generated transcripts of the transposase gene in vitro and co-transfected them with donor vector plasmids, resulting in high copy number integrations and expression levels of the gene of interest (GOI). We anticipate that the use of transposase mRNA will contribute to further improvements in cell line development processes.

MOLECULAR BIOTECHNOLOGY (2023)

Add to Collection

Article Genetics & Heredity

Noise-Reduction and Sensitivity-Enhancement of a Sleeping Beauty-Based Tet-On System

Sarah C. Saunderson, S. M. Ali Hosseini-Rad, Alexander D. McLellan

Summary: Tetracycline-inducible systems are widely used control elements for mammalian gene expression. The G72V mutation in the rtTA-M2 tetracycline activator reduces basal level expression and increases fold-induction. Sensitivity enhancing mutations compensate for the loss of responsiveness caused by the G72V mutation without compromising promoter tightness.

GENES (2022)

Add to Collection

Article Biochemistry & Molecular Biology

SB Digestor: a tailored driver gene identification tool for dissecting heterogeneous Sleeping Beauty transposon- induced tumors

Aiping Zhang, Lijian Wang, Josh Haipeng Lei, Zhengqiang Miao, Monica Vishnu Valecha, Peng Hu, Kai Miao, Chu-Xia Deng

Summary: SB Digestor is a new computational algorithm that overcomes biological heterogeneity and accurately detects and defines cancer driver genes, providing more potential candidates for study.

INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES (2023)

Add to Collection

Review Oncology

Identification of cancer driver genes using Sleeping Beauty transposon mutagenesis

Haruna Takeda, Nancy A. Jenkins, Neal G. Copeland

Summary: Cancer genome sequencing studies have identified driver genes for various cancers, but identifying these genes solely from genetic data remains challenging. In vivo forward genetic screens using SB transposon mutagenesis in mice provide a powerful tool for identifying potential cancer driver genes. Comparing driver genes identified in human and mouse tumors can enhance confidence in identifying cancer driver genes.

CANCER SCIENCE (2021)

Add to Collection

Article Genetics & Heredity

The Integration Preference of Sleeping Beauty at Non-TA Site Is Related to the Transposon End Sequences

Yiting Zhou, Guangwei Ma, Jiawen Yang, Zenghong Gao, Yabin Guo

Summary: Recent study showed that Sleeping Beauty transposon can integrate into non-TA sites, mainly through aberrant integrations. The consensus sequence of the non-TA target sites corresponds to the transposon end sequence.

FRONTIERS IN GENETICS (2021)

Add to Collection

Review Virology

Jumping Ahead with Sleeping Beauty: Mechanistic Insights into Cut-and-Paste Transposition

Matthias T. Ochmann, Zoltan Ivics

Summary: The Sleeping Beauty transposon system has been widely used as a genetic engineering tool, with extensive studies elucidating the molecular mechanisms and actions during SB transposition process. Structural data on the SB transposase provide a direct insight into the enzyme's functionality, leading to the development of novel SB variants for advanced genetic engineering possibilities. However, many aspects of transposition process remain poorly understood, necessitating further research to explore the structure-function relationships of SB transposition for the development of new vector systems.

VIRUSES-BASEL (2021)

Add to Collection

Article Medicine, Research & Experimental

Functionalized PDA/DEX-PEI@HA nanoparticles combined with sleeping-beauty transposons for multistage targeted delivery of CRISPR/Cas9 gene

Kun Ma, Wenzhe Li, Guang Zhu, Shibo Sun, Hao Chi, Yalin Yin, He Diao, Xiao-Jin Xing, Zhaoming Guo, Li Wang, Weiping Xu, Changhao Cui, Jianqiang Xu

Summary: The CRISPR/Cas9 gene was cloned into a non-viral safe vector Sleeping-Beauty (SB) transposon to construct a safer vector. In addition, PDA/DEX-PEI@HA (PDPH) nanoparticles were developed to facilitate precise CRISPR/Cas9 targeted delivery, showing high transfection efficiency and minimal cytotoxicity. The study highlights the potential of PDA/DEX-PEI25k@HA/pT25pCas9 (PDPH25k/pT(25K)pCas9) as a non-viral gene vector for CRISPR/Cas9 delivery in clinical medication.

BIOMEDICINE & PHARMACOTHERAPY (2021)

Add to Collection

Article Multidisciplinary Sciences

Understanding cancer drug resistance with Sleeping Beauty functional genomic screens: Application to MAPK inhibition in cutaneous melanoma

Eliot Y. Zhu, Jacob L. Schillo, Sarina D. Murray, Jesse D. Riordan, Adam J. Dupuy

Summary: Combined BRAF and MEK inhibition is an effective treatment for BRAF-mutant cutaneous melanoma, but drug resistance remains a challenge. This study utilized the Sleeping Beauty transposon system to investigate the mechanisms of drug resistance in melanoma. The findings demonstrated the importance of considering melanoma heterogeneity and identified a potential target for overcoming drug resistance.

ISCIENCE (2023)

Add to Collection

Review Biochemistry & Molecular Biology

Contemporary Transposon Tools: A Review and Guide through Mechanisms and Applications of Sleeping Beauty, piggyBac and Tol2 for Genome Engineering

Nicolas Sandoval-Villegas, Wasifa Nurieva, Maximilian Amberger, Zoltan Ivics

Summary: Transposons are mobile genetic elements that have been used as experimental tools to introduce foreign DNA sequences into target cells in a regulated and highly efficient manner. They are supplied as naked nucleic acids or recombinant proteins, making their use simple, safe, and economically competitive. Transposons enable various avenues for genome manipulations in vertebrates.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

Add to Collection

Article Biotechnology & Applied Microbiology

Improvement of Sleeping Beauty Transposon System Enabling Efficient and Stable Protein Production

Yun Haeng Lee, Ji Yun Park, Eun Seon Song, Haneur Lee, Myeong Uk Kuk, Junghyun Joo, Hyungmin Roh, Joon Tae Park

Summary: This study proposes a new strategy to increase the efficiency and stability of protein production by modifying the traditional SB transposon vector. Adding a pair of inverted terminal repeats (ITRs) next to existing ITRs significantly improves transgene integration efficiency, resulting in high-yield and sustained protein production. Moreover, double-ITRs show a more favorable response to DNA methylation inhibitors, suggesting their potential in increasing protein productivity.

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING (2022)

Add to Collection

Article Biochemistry & Molecular Biology

Molecular and Functional Characterization of BDNF-Overexpressing Human Retinal Pigment Epithelial Cells Established by Sleeping Beauty Transposon-Mediated Gene Transfer

Larissa Mattern, Katrin Otten, Csaba Miskey, Matthias Fuest, Zsuzsanna Izsvak, Zoltan Ivics, Peter Walter, Gabriele Thumann, Sandra Johnen

Summary: This study presents a potential treatment option for dry AMD by transferring the BDNF gene into RPE cells. The expression of BDNF in the transfected cells leads to reduced apoptosis rates and stimulated neurite outgrowth.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

Add to Collection

Article Biochemistry & Molecular Biology

Construction and Quantitative Evaluation of a Tissue-Specific Sleeping Beauty by EDL2-Specific Transposase Expression in Esophageal Squamous Carcinoma Cell Line KYSE-30

Reihaneh Alsadat Mahmoudian, Fardin Fathi, Moein Farshchian, Mohammad Reza Abbaszadegan

Summary: The Sleeping Beauty transposon system is an efficient tool for gene delivery to esophageal tissue. In this study, the authors combined the hyperactive SB100X transposase with the ED-L2 tissue-specific promoter to achieve stable expression of the T2-Onc3 transposon in esophageal squamous epithelial cells.

MOLECULAR BIOTECHNOLOGY (2023)

Add to Collection

Article Cell Biology

Sleeping Beauty kit sets provide rapid and accessible generation of artificial antigen-presenting cells for natural killer cell expansion

Lachlan J. Dobson, Sarah C. Saunderson, Samuel W. J. Smith-Bell, Alexander D. McLellan

Summary: Artificial antigen-presenting cells (aAPCs) are cost-effective and convenient tools for expanding CAR-bearing T cells and NK cells. We developed a single-step technique using Sleeping Beauty (SB)-based vectors with antibiotic selection to overcome difficulties in accessing existing aAPC lines and isolate stable aAPC clones. Our SB vectors allow for the surface expression of CD86, 4-1BBL, membrane-bound (mb) interleukin (IL)-15, and mbIL-21 in aAPCs, which successfully expanded NK cells to high purity. The multivector kit set we developed enables in-house production of effective aAPCs for the in vitro expansion of primary cells.

IMMUNOLOGY AND CELL BIOLOGY (2023)

Add to Collection

Article Biology

Doxycycline-inducible Expression of Proteins at Near-endogenous Levels in Mammalian Cells Using the Sleeping Beauty Transposon System

Karolina Zak, Costin N. Antonescu

Summary: The function and localization of a protein in a cell can be determined by studying its interaction with other proteins and its subcellular localization. Expressing mutants of the protein with specific perturbations in protein interaction motifs or fluorescent protein fusions can help understand its mechanism of action and localization. However, tight regulation is needed to avoid potential alterations in protein interactions or localizations that can result from protein overexpression.

BIO-PROTOCOL (2023)

Add to Collection

Article Biochemistry & Molecular Biology

Cellular expression and function of naturally occurring variants of the human ABCG2 multidrug transporter

Boglarka Zambo, Orsolya Mozner, Zsuzsa Bartos, Gyorgy Torok, Gyorgy Varady, Agnes Telbisz, Laszlo Homolya, Tamas I. Orban, Balazs Sarkadi

CELLULAR AND MOLECULAR LIFE SCIENCES (2020)

Add to Collection

Article Pharmacology & Pharmacy

Precision-engineered reporter cell lines reveal ABCG2 regulation in live lung cancer cells

Daniella Kovacsics, Anna Brozik, Borbala Tihanyi, Zsolt Matula, Adrienn Borsy, Nikolett Meszaros, Edit Szabo, Eszter Nemeth, Abel Fothi, Boglarka Zambo, David Szuts, Gyorgy Varady, Tamas Orban, Agota Apati, Balazs Sarkadi

BIOCHEMICAL PHARMACOLOGY (2020)

Add to Collection

Article Pharmacology & Pharmacy

Combination of Alanine and Glutathione as Targeting Ligands of Nanoparticles Enhances Cargo Delivery into the Cells of the Neurovascular Unit

Gergo Porkolab, Maria Meszaros, Andras Toth, Aniko Szecsko, Andras Harazin, Zsolt Szegletes, Gyorgyi Ferenc, Andras Blastyak, Lajos Mates, Gabor Rakhely, Maria A. Deli, Szilvia Veszelka

PHARMACEUTICS (2020)

Add to Collection

Article Biochemistry & Molecular Biology

Tissue-specific and transcription-dependent mechanisms regulate primary microRNA processing efficiency of the human chromosome 19 MicroRNA cluster

Abel Fothi, Orsolya Biro, Zsuzsa Erdei, Agota Apati, Tamas I. Orban

Summary: The study reveals tissue-specific and position-dependent differences in expression levels of C19MC miRNA cluster in embryonic stem cells and placenta, possibly due to differences in posttranscriptional processing. In placenta, there are enhancer regions associated with the Microprocessor complex, leading to more efficient processing of pri-miRNA.

RNA BIOLOGY (2021)

Add to Collection

Article Hematology

Single-dose MGTA-145/plerixafor leads to efficient mobilization and in vivo transduction of HSCs with thalassemia correction in mice

Chang Li, Kevin A. Goncalves, Tamas Rasko, Amit Pande, Sucheol Gil, Zhinan Liu, Zsuzsanna Izsvak, Thalia Papayannopoulou, John C. Davis, Hans-Peter Kiem, Andre Lieber

Summary: A new in vivo hemopoietic stem cell gene therapy approach has been developed without the need for myelosuppressive conditioning and autologous HSC transplantation, involving HSC mobilization and a helper-dependent adenovirus vector system. The mobilization regimen using truncated GRO-beta and plerixafor resulted in robust HSC mobilization with less toxicity. In a mouse model, in vivo HSC transduction after mobilization led to complete correction of thalassemia, demonstrating the potential for long-term HSC repopulation.

BLOOD ADVANCES (2021)

Add to Collection

Article Genetics & Heredity

Posttranscriptional Regulation of the Human ABCG2 Multidrug Transporter Protein by Artificial Mirtrons

Anita Schamberger, Gyorgy Varady, Abel Fothi, Tamas Orban

Summary: ABCG2 is a membrane transporter protein associated with multidrug resistance and tumor development. By designing artificial mirtrons, researchers were able to posttranscriptionally regulate ABCG2 expression, with mismatch at the first position resulting in better silencing. Additionally, a mismatch in the seed region did not impair efficient silencing at the protein level.

GENES (2021)

Add to Collection

Article Biology

A versatile transposon-based technology to generate loss- and gain-of-function phenotypes in the mouse liver

Anna Georgina Kopasz, David Zsolt Pusztai, Reka Karkas, Liza Hudoba, Khaldoon Sadiq Ahmed Abdullah, Gergely Imre, Gabriella Pankotai-Bodo, Ede Migh, Andrea Nagy, Andras Kriston, Peter German, Andrea Bakne Drubi, Anna Molnar, Ildiko Fekete, Virag Eva Dani, Imre Ocsovszki, Laszlo Geza Puskas, Peter Horvath, Farkas Sukosd, Lajos Mates

Summary: A versatile technology platform for in vivo somatic genome editing in the mouse liver has been developed, meeting multiple requirements for long-lasting high-level transgene expression. This technology is believed to contribute to the development of a more accurate new generation of tools for gene function analysis in mice.

BMC BIOLOGY (2022)

Add to Collection

Article Genetics & Heredity

Functional indications for transposase domestications - Characterization of the human piggyBac transposase derived (PGBD) activities

Orsolya Kolacsek, Gerda Wachtl, Abel Fothi, Anita Schamberger, Sara Sandor, Eniko Pergel, Nora Varga, Tamas Rasko, Zsuzsanna Izsvak, Agota Apati, Tamas I. Orban

Summary: The study suggests that human PGBD elements did not retain their mobilizing activity, but exhibit cell type specific and cellular stress related expression patterns, indicating potential distinct domesticated functions.

GENE (2022)

Add to Collection

Article Biochemistry & Molecular Biology

Molecular and Functional Characterization of BDNF-Overexpressing Human Retinal Pigment Epithelial Cells Established by Sleeping Beauty Transposon-Mediated Gene Transfer

Larissa Mattern, Katrin Otten, Csaba Miskey, Matthias Fuest, Zsuzsanna Izsvak, Zoltan Ivics, Peter Walter, Gabriele Thumann, Sandra Johnen

Summary: This study presents a potential treatment option for dry AMD by transferring the BDNF gene into RPE cells. The expression of BDNF in the transfected cells leads to reduced apoptosis rates and stimulated neurite outgrowth.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

Add to Collection

Article Biochemistry & Molecular Biology

Functional Characterization of the N-Terminal Disordered Region of the piggyBac Transposase

Gerda Wachtl, Eva Schad, Krisztina Huszar, Antonio Palazzo, Zoltan Ivics, Agnes Tantos, Tamas Orban

Summary: The N-terminal disordered region (NTDR) of piggyBac DNA transposon plays an important role in the transposition process. Deletion of the NTDR significantly impairs piggyBac transposition, with varying effects in different cell types. The conservation of NTDR sequence is crucial for rescuing transposase activity, and the excision step of transposition is more severely affected by NTDR deletion. Bioinformatics analysis reveals that NTDR is specific for the piggyBac superfamily and is also present in transposase-derived proteins that are incapable of catalyzing transposition. These findings indicate the essential role of NTDR in fine-tuning transposition and its significance in the functions of piggyBac-originated co-opted genes.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

Add to Collection

Article Genetics & Heredity

Partial Disturbance of Microprocessor Function in Human Stem Cells Carrying a Heterozygous Mutation in the DGCR8 Gene

Dora Ree, Abel Fothi, Nora Varga, Orsolya Kolacsek, Tamas Orban, Agota Apati

Summary: Studies have shown that DGCR8 heterozygous mutation has a modest effect on mRNA level but a significant decrease at the protein level, while not affecting self-renewal and trilineage differentiation capacity of hESCs.

GENES (2022)

Add to Collection

Article Biochemistry & Molecular Biology

GMP-Grade Manufacturing and Quality Control of a Non-Virally Engineered Advanced Therapy Medicinal Product for Personalized Treatment of Age-Related Macular Degeneration

Martina Kropp, Nina Harmening, Thais Bascuas, Sandra Johnen, Eline De Clerck, Veronica Fernandez, Mattia Ronchetti, Ruggero Cadossi, Cristina Zanini, Daniel Scherman, Zoltan Ivics, Corinne Marie, Zsuzsanna Izsvak, Gabriele Thumann

Summary: The introduction of new therapeutics requires validation of Good Manufacturing Practice (GMP)-grade manufacturing including suitable quality controls. This study presents a personalized, cell-based gene therapy for age-related macular degeneration and establishes a validation strategy for GMP-grade manufacturing and quality control of Advanced Therapy Medicinal Products (ATMP). The challenges of low cell number, batch-to-batch variability, and short production duration were overcome through the use of human donor tissue and an extended validation process.

BIOMEDICINES (2022)

Add to Collection

Article Biochemistry & Molecular Biology

Enhanced Biosafety of the Sleeping Beauty Transposon System by Using mRNA as Source of Transposase to Efficiently and Stably Transfect Retinal Pigment Epithelial Cells

Nina Harmening, Sandra Johnen, Zsuzsanna Izsvak, Zoltan Ivics, Martina Kropp, Thais Bascuas, Peter Walter, Andreas Kreis, Bojan Pajic, Gabriele Thumann

Summary: Neovascular age-related macular degeneration (nvAMD) leads to blindness if untreated and is treated with frequent intravitreal injections of anti-angiogenic biopharmaceuticals. Our laboratory is developing a cell-based gene therapy using autologous RPE cells transfected with PEDF gene to inhibit blood vessel growth. We investigated the use of SB100X transposase delivered as mRNA and successfully transfected ARPE-19 cells and primary human RPE cells with Venus or PEDF gene, resulting in long-term transgene expression.

BIOMOLECULES (2023)

Add to Collection

Review Biology

One locus, several functional RNAs-emerging roles of the mechanisms responsible for the sequence variability of microRNAs

Tamas I. Orban

Summary: With the development of modern molecular genetics, the one gene-one enzyme hypothesis has been replaced by more complex mechanisms. Alternative splicing, RNA editing, and other processes contribute to the variability of RNA species produced by protein-coding and non-protein coding genes. This review focuses on the mechanisms underlying the astonishing variability of microRNAs (miRNAs) and how it contributes to molecular variability in living organisms and potential therapeutic applications for human diseases.

BIOLOGIA FUTURA (2023)

Add to Collection

Article Virology

Human Endogenous Retrovirus K Rec Forms a Regulatory Loop with MITF that Opposes the Progression of Melanoma to an Invasive Stage

Manvendra Singh, Huiqiang Cai, Mario Bunse, Cedric Feschotte, Zsuzsanna Izsvak

VIRUSES-BASEL (2020)

Add to Collection

No Data Available

© Peeref 2019-2024. All rights reserved.