The great escape: how cationic polyplexes overcome the endosomal barrier

Determining the effects of PEI adsorption on the permeability of 1,2-dipalmitoylphosphatidylcholine/bis(monoacylglycero)phosphate membranes under osmotic stress

(2018) Scott R. Clark et al.   Acta Biomaterialia

Beyond Gene Transfection with Methacrylate-Based Polyplexes—The Influence of the Amino Substitution Pattern

(2018) Anne-Kristin Trützschler et al.   BIOCONJUGATE CHEMISTRY

Zinc Coordination Substitute Amine: A Noncationic Platform for Efficient and Safe Gene Delivery

(2018) Shuai Liu et al.   ACS Macro Letters

Rapid and facile quantitation of polyplex endocytic trafficking

(2017) Mihael Lazebnik et al.   JOURNAL OF CONTROLLED RELEASE

Delivery technologies for genome editing

(2017) Hao Yin et al.   NATURE REVIEWS DRUG DISCOVERY

Membrane pores: from structure and assembly, to medicine and technology

(2017) Robert J. C. Gilbert et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

3rd generation poly(ethylene imine)s for gene delivery

(2017) Tanja Bus et al.   Journal of Materials Chemistry B

Degradable Polyethylenimine-Based Gene Carriers for Cancer Therapy

(2017) Hu-Lin Jiang et al.   Topics in Current Chemistry

History of Polymeric Gene Delivery Systems

(2017) Peng Zhang et al.   Topics in Current Chemistry

Role of Cell Membrane–Vector Interactions in Successful Gene Delivery

(2016) Sriram Vaidyanathan et al.   ACCOUNTS OF CHEMICAL RESEARCH

Porous Silicon and Polymer Nanocomposites for Delivery of Peptide Nucleic Acids as Anti-MicroRNA Therapies

(2016) Kelsey R. Beavers et al.   ADVANCED MATERIALS

Mechanism of Enhanced Cellular Uptake and Cytosolic Retention of MK2 Inhibitory Peptide Nano-polyplexes

(2016) Kameron V. Kilchrist et al.   Cellular and Molecular Bioengineering

Quantitative study of effects of free cationic chains on gene transfection in different intracellular stages

(2016) Jinge Cai et al.   JOURNAL OF CONTROLLED RELEASE

Interactions of Polyethylenimines with Zwitterionic and Anionic Lipid Membranes

(2016) Urszula Kwolek et al.   LANGMUIR

Cationic Polymer Intercalation into the Lipid Membrane Enables Intact Polyplex DNA Escape from Endosomes for Gene Delivery

(2016) Sriram Vaidyanathan et al.   MOLECULAR PHARMACEUTICS

Intracellular trafficking and exocytosis of a multi-component siRNA nanocomplex

(2016) Ravi S. Shukla et al.   Nanomedicine-Nanotechnology Biology and Medicine

Engineering Delivery Vehicles for Genome Editing

(2016) Christopher E. Nelson et al.   Annual Review of Chemical and Biomolecular Engineering

Unusual Salt and pH Induced Changes in Polyethylenimine Solutions

(2016) Kimberly A. Curtis et al.   PLoS One

Quantitative Measurement of Cationic Polymer Vector and Polymer–pDNA Polyplex Intercalation into the Cell Plasma Membrane

(2015) Sriram Vaidyanathan et al.   ACS Nano

Self-Assembled DNA Nanoclews for the Efficient Delivery of CRISPR-Cas9 for Genome Editing

(2015) Wujin Sun et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Rapid Exchange Between Free and Bound States in RNA–Dendrimer Polyplexes: Implications on the Mechanism of Delivery and Release

(2015) Anisha Shakya et al.   BIOMACROMOLECULES

Nucleic Acid Therapeutics Using Polyplexes: A Journey of 50 Years (and Beyond)

(2015) Ulrich Lächelt et al.   CHEMICAL REVIEWS

Challenges in CRISPR/CAS9 Delivery: Potential Roles of Nonviral Vectors

(2015) Ling Li et al.   HUMAN GENE THERAPY

A review of the developments of characteristics of PEI derivatives for gene delivery applications

(2015) Shahrouz Taranejoo et al.   JOURNAL OF APPLIED POLYMER SCIENCE

Multifunctional poly(methacrylate) polyplex libraries: A platform for gene delivery inspired by nature

(2015) M.E. Favretto et al.   JOURNAL OF CONTROLLED RELEASE

Building the foundation for genomics in precision medicine

(2015) Samuel J. Aronson et al.   NATURE

Visualizing lipid-formulated siRNA release from endosomes and target gene knockdown

(2015) Anders Wittrup et al.   NATURE BIOTECHNOLOGY

The endosomal-lysosomal system: from acidification and cargo sorting to neurodegeneration

(2015) Yong-Bo Hu et al.   Translational Neurodegeneration

Investigating the Effects of Block versus Statistical Glycopolycations Containing Primary and Tertiary Amines for Plasmid DNA Delivery

(2014) Dustin Sprouse et al.   BIOMACROMOLECULES

Synthesis of Poly(meth)acrylates with Thioether and Tertiary Sulfonium Groups by ARGET ATRP and Their Use as siRNA Delivery Agents

(2014) Matthew C. Mackenzie et al.   BIOMACROMOLECULES

Gene transfection in complex media using PCBMAEE-PCBMA copolymer with both hydrolytic and zwitterionic blocks

(2014) Juan Zhang et al.   BIOMATERIALS

Autophagy Induced by Calcium Phosphate Precipitates Targets Damaged Endosomes

(2014) Xi Chen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Insight into nanoparticle cellular uptake and intracellular targeting

(2014) Basit Yameen et al.   JOURNAL OF CONTROLLED RELEASE

Interaction of Polyethylenimine with Model Cell Membranes Studied by Linear and Nonlinear Spectroscopic Techniques

(2014) Chi Zhang et al.   Journal of Physical Chemistry C

Intracellular delivery of nanomaterials: How to catch endosomal escape in the act

(2014) Thomas F. Martens et al.   Nano Today

Mechanism of Polyplex- and Lipoplex-Mediated Delivery of Nucleic Acids: Real-Time Visualization of Transient Membrane Destabilization without Endosomal Lysis

(2013) Zia ur Rehman et al.   ACS Nano

Versatile functionalization of gene vectors via different types of zwitterionic betaine species for serum-tolerant transfection

(2013) Ke-Mao Xiu et al.   Acta Biomaterialia

Conjugation with Betaine: A Facile and Effective Approach to Significant Improvement of Gene Delivery Properties of PEI

(2013) Jun Sun et al.   BIOMACROMOLECULES

Characterization of Conformation and Interaction of Gene Delivery Vector Polyethylenimine with Phospholipid Bilayer at Different Protonation State

(2013) Chandan Kumar Choudhury et al.   BIOMACROMOLECULES

History of gene therapy

(2013) Thomas Wirth et al.   GENE

Independent versus Cooperative Binding in Polyethylenimine–DNA and Poly(l-lysine)–DNA Polyplexes

(2013) Tiia-Maaria Ketola et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Polyplex-Induced Cytosolic Nuclease Activation Leads to Differential Transgene Expression

(2013) Rahul Rattan et al.   MOLECULAR PHARMACEUTICS

Overcoming Nonviral Gene Delivery Barriers: Perspective and Future

(2013) Charles H. Jones et al.   MOLECULAR PHARMACEUTICS

Polyplex Exposure Inhibits Cell Cycle, Increases Inflammatory Response, and Can Cause Protein Expression without Cell Division

(2013) Rebecca L. Matz et al.   MOLECULAR PHARMACEUTICS

Structural and dynamical properties of polyethylenimine in explicit water at different protonation states: a molecular dynamics study

(2013) Chandan Kumar Choudhury et al.   Soft Matter

Synthesis and Properties of Sulfonium Polyelectrolytes for Biological Applications

(2013) Sean T. Hemp et al.   ACS Macro Letters

Poly(2-deoxy-2-methacrylamido glucopyranose)-b-Poly(methacrylate amine)s: Optimization of Diblock Glycopolycations for Nucleic Acid Delivery

(2013) Haibo Li et al.   ACS Macro Letters

Ex Vivo Red Blood Cell Hemolysis Assay for the Evaluation of pH-responsive Endosomolytic Agents for Cytosolic Delivery of Biomacromolecular Drugs

(2013) Brian C. Evans et al.   Jove-Journal of Visualized Experiments

Nucleic Acid Delivery: The Missing Pieces of the Puzzle?

(2012) Juliane Nguyen et al.   ACCOUNTS OF CHEMICAL RESEARCH

Multifunctional triblock copolymers for intracellular messenger RNA delivery

(2012) Connie Cheng et al.   BIOMATERIALS

Branched and linear poly(ethylene imine)-based conjugates: synthetic modification, characterization, and application

(2012) Michael Jäger et al.   CHEMICAL SOCIETY REVIEWS

Crosslinked linear polyethylenimine enhances delivery of DNA to the cytoplasm

(2012) Daniel K. Bonner et al.   JOURNAL OF CONTROLLED RELEASE

Polyphosphonium Polymers for siRNA Delivery: An Efficient and Nontoxic Alternative to Polyammonium Carriers

(2012) Cátia Ornelas-Megiatto et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Polyelectrolyte Complexes of DNA and Linear PEI: Formation, Composition and Properties

(2012) Igor Y. Perevyazko et al.   LANGMUIR

Polyplexes Traffic through Caveolae to the Golgi and Endoplasmic Reticulum en Route to the Nucleus

(2012) Meghan J. Reilly et al.   MOLECULAR PHARMACEUTICS

The Possible “Proton Sponge ” Effect of Polyethylenimine (PEI) Does Not Include Change in Lysosomal pH

(2012) Rikke V Benjaminsen et al.   MOLECULAR THERAPY

Galectin 8 targets damaged vesicles for autophagy to defend cells against bacterial invasion

(2012) Teresa L. M. Thurston et al.   NATURE

Insights into the endosomal escape mechanism via investigation of dendrimer–membrane interactions

(2012) Wen-de Tian et al.   Soft Matter

Progress and perspectives in developing polymeric vectors for in vitro gene delivery

(2012) Yanan Yue et al.   Biomaterials Science

Polymers for siRNA Delivery: Inspired by Viruses to be Targeted, Dynamic, and Precise

(2011) Ernst Wagner   ACCOUNTS OF CHEMICAL RESEARCH

Synthesis of PAMAM Dendrimer Derivatives with Enhanced Buffering Capacity and Remarkable Gene Transfection Efficiency

(2011) Gwang Sig Yu et al.   BIOCONJUGATE CHEMISTRY

Intracellular Trafficking of Polyamidoamine–Poly(ethylene glycol) Block Copolymers in DNA Delivery

(2011) Daniel K. Bonner et al.   BIOCONJUGATE CHEMISTRY

Phosphonium-Containing Polyelectrolytes for Nonviral Gene Delivery

(2011) Sean T. Hemp et al.   BIOMACROMOLECULES

Diblock Glycopolymers Promote Colloidal Stability of Polyplexes and Effective pDNA and siRNA Delivery under Physiological Salt and Serum Conditions

(2011) Adam E. Smith et al.   BIOMACROMOLECULES

Elucidating the interplay between DNA-condensing and free polycations in gene transfection through a mechanistic study of linear and branched PEI

(2011) Zhuojun Dai et al.   BIOMATERIALS

Molecular Dynamics Simulations of DNA/PEI Complexes: Effect of PEI Branching and Protonation State

(2011) Chongbo Sun et al.   BIOPHYSICAL JOURNAL

Rational design of biodegradable cationic polycarbonates for gene delivery

(2011) Zhan Yuin Ong et al.   JOURNAL OF CONTROLLED RELEASE

Revisit complexation between DNA and polyethylenimine — Effect of length of free polycationic chains on gene transfection

(2011) Yanan Yue et al.   JOURNAL OF CONTROLLED RELEASE

Protein kinase A inhibition modulates the intracellular routing of gene delivery vehicles in HeLa cells, leading to productive transfection

(2011) Zia ur Rehman et al.   JOURNAL OF CONTROLLED RELEASE

Lysosomal pH and analysis of the counter ion pathways that support acidification

(2011) Jessica E. DiCiccio et al.   JOURNAL OF GENERAL PHYSIOLOGY

A Discussion of the pH-Dependent Protonation Behaviors of Poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and Poly(ethylenimine-ran-2-ethyl-2-oxazoline) (P(EI-r-EOz))

(2011) Hoyoung Lee et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Odd–Even Effect of Repeating Aminoethylene Units in the Side Chain of N-Substituted Polyaspartamides on Gene Transfection Profiles

(2011) Hirokuni Uchida et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Novel Biodegradable Block Copolymers of Poly(ethylene glycol) (PEG) and Cationic Polycarbonate: Effects of PEG Configuration on Gene Delivery

(2011) Chuan Yang et al.   MACROMOLECULAR RAPID COMMUNICATIONS

Interaction of Poly(ethylenimine)–DNA Polyplexes with Mitochondria: Implications for a Mechanism of Cytotoxicity

(2011) Giovanna Grandinetti et al.   MOLECULAR PHARMACEUTICS

Membrane and Nuclear Permeabilization by Polymeric pDNA Vehicles: Efficient Method for Gene Delivery or Mechanism of Cytotoxicity?

(2011) Giovanna Grandinetti et al.   MOLECULAR PHARMACEUTICS

Induction of genuine autophagy by cationic lipids in mammalian cells

(2010) Na Man et al.   Autophagy

Glucosamine induces autophagy via an mTOR-independent pathway

(2010) Tomoya Shintani et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Enhanced gene transfection and serum stability of polyplexes by PDMAEMA-polysulfobetaine diblock copolymers

(2010) Fengying Dai et al.   BIOMATERIALS

Cyclodextrin-based gene delivery systems

(2010) Carmen Ortiz Mellet et al.   CHEMICAL SOCIETY REVIEWS

Progress and prospects: nuclear import of nonviral vectors

(2010) A P Lam et al.   GENE THERAPY

Breaking down the barriers: siRNA delivery and endosome escape

(2010) M. Dominska et al.   JOURNAL OF CELL SCIENCE

Photochemical internalization provides time- and space-controlled endolysosomal escape of therapeutic molecules

(2010) Pål Kristian Selbo et al.   JOURNAL OF CONTROLLED RELEASE

Revisit complexation between DNA and polyethylenimine — Effect of uncomplexed chains free in the solution mixture on gene transfection

(2010) Yanan Yue et al.   JOURNAL OF CONTROLLED RELEASE

Endosomal escape pathways for delivery of biologicals

(2010) Amir K. Varkouhi et al.   JOURNAL OF CONTROLLED RELEASE

Autophagy: cellular and molecular mechanisms

(2010) Danielle Glick et al.   JOURNAL OF PATHOLOGY

Cationic methacrylate copolymers containing primary and tertiary amino side groups: Controlled synthesis via RAFT polymerization, DNA condensation, and in vitro gene transfection

(2010) Caihong Zhu et al.   JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY

Polycation-Induced Cell Membrane Permeability Does Not Enhance Cellular Uptake or Expression Efficiency of Delivered DNA

(2010) Lisa E. Prevette et al.   MOLECULAR PHARMACEUTICS

Understanding the Protonation Behavior of Linear Polyethylenimine in Solutions through Monte Carlo Simulations

(2009) Jesse D. Ziebarth et al.   BIOMACROMOLECULES

Chemical vectors for gene delivery: a current review on polymers, peptides and lipids containing histidine or imidazole as nucleic acids carriers

(2009) Patrick Midoux et al.   BRITISH JOURNAL OF PHARMACOLOGY

Efficient polyethylenimine-mediated gene delivery proceeds via a caveolar pathway in HeLa cells

(2009) Nathan P. Gabrielson et al.   JOURNAL OF CONTROLLED RELEASE

Missing pieces in understanding the intracellular trafficking of polycation/DNA complexes

(2009) You-Yeon Won et al.   JOURNAL OF CONTROLLED RELEASE

Cationic Shell-Cross-Linked Knedel-like (cSCK) Nanoparticles for Highly Efficient PNA Delivery

(2009) Huafeng Fang et al.   MOLECULAR PHARMACEUTICS

Performance of three PDMAEMA-based polycation architectures as gene delivery agents in comparison to linear and branched PEI

(2009) Anja Schallon et al.   REACTIVE & FUNCTIONAL POLYMERS

Macroautophagy in Immunity and Tolerance

(2009) Monique Gannagé et al.   TRAFFIC

Polymer-Based Gene Delivery: A Current Review on the Uptake and Intracellular Trafficking of Polyplexes

(2008) Patrick Midoux et al.   CURRENT GENE THERAPY

Current prospects for mRNA gene delivery

(2008) Ayako Yamamoto et al.   EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

Release of cationic polymer-DNA complexes from the endosome: A theoretical investigation of the proton sponge hypothesis

(2008) Shuang Yang et al.   JOURNAL OF CHEMICAL PHYSICS

Polyplexes from Poly(aspartamide) Bearing 1,2-Diaminoethane Side Chains Induce pH-Selective, Endosomal Membrane Destabilization with Amplified Transfection and Negligible Cytotoxicity

(2008) Kanjiro Miyata et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Membrane lipids: where they are and how they behave

(2008) Gerrit van Meer et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Biodegradable polymers as non-viral carriers for plasmid DNA delivery

(2007) Jordy Luten et al.   JOURNAL OF CONTROLLED RELEASE