Article
Dentistry, Oral Surgery & Medicine
Na Young Shim, Jae-In Ryu, Jung Sun Heo
Summary: The study demonstrated that fucoidan stimulates osteogenic differentiation of periodontal ligament stem cells (PDLSCs) through the PI3K/Akt and Wnt/beta-catenin pathways, enhancing bone formation.
Article
Biochemistry & Molecular Biology
Hang Yuan, Shigeki Suzuki, Shizu Hirata-Tsuchiya, Akiko Sato, Eiji Nemoto, Masahiro Saito, Hideki Shiba, Satoru Yamada
Summary: PPAR gamma is identified as the key transcriptional factor maintaining the osteo/cementogenic abilities of periodontal ligament fibroblasts, with its effects mediated by global H3K27ac modifications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Multidisciplinary
Ryoya Iizumi, Michiyo Honda
Summary: This study aimed to investigate the inhibitory effects of Wnt/β-catenin signaling on osteogenic differentiation in human periodontal ligament fibroblasts (HPLFs). The researchers found that Wnt-3a activation of Wnt/β-catenin signaling inhibited the formation of bone nodules and suppressed the expression of osteogenic differentiation-related genes in HPLFs.
Article
Cell Biology
Qian Lan, Jiadong Cao, Xueting Bi, Xin Xiao, Dongsheng Li, Yilong Ai
Summary: This study suggests that using Cur-PDLSC-EV as a curcumin carrier can promote osteogenic differentiation of periodontal ligament stem cells, providing potential therapeutic value for periodontal diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Ming Ying, Bin Zhang
Summary: This study investigated the effect of daidzein on the proliferation and osteogenic differentiation of human periodontal ligament stem cells. It was found that daidzein promotes these processes by activating the Wnt/β-catenin signaling pathway.
Article
Cell Biology
Ruiyao Xu, Xianxian Wang, Sadia Safi, Nico Braunegger, Agnes Hipgrave Ederveen, Michelle Rottmann, Joachim Wittbrodt, Manfred Wuhrer, Janine Wesslowski, Gary Davidson
Summary: Reception of Wnt signals is mediated by Frizzled receptors and a co-receptor LRP6 or LRP5. Precise control of receptor activation is crucial to regulate Wnt/beta-catenin signaling for proper cellular function. A glycosyltransferase B3GnT2-like has been identified to modify LRP6 and enhance Wnt/beta-catenin signaling. This study highlights the importance of LRP6 as a regulatory hub in Wnt signaling and provides insight into the selective targeting of a signaling pathway component by a specific glycosyltransferase.
Article
Dentistry, Oral Surgery & Medicine
Panpan Yang, Congshan Li, Yuying Kou, Yujun Jiang, Dongfang Li, Shanshan Liu, Yupu Lu, Tomoka Hasegawa, Minqi Li
Summary: The study showed that Notum inhibits the osteogenic differentiation of hPDLSCs possibly through the Wnt/Beta-catenin downstream signaling pathway.
ARCHIVES OF ORAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Madoka Yasunaga, Hiroyuki Ishikawa, Sachio Tamaoki, Hidefumi Maeda, Jun Ohno
Summary: Culturing HPLSCs as spheroids embedded in collagen gel with rhPAI-1 treatment enhances their cementogenic differentiation. The interaction between HPLSC spheroids and collagenous extracellular matrix is promoted by rhPAI-1, leading to increased cell migration and spread.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Jing Zhao, Louis Faure, Igor Adameyko, Paul T. Sharpe
Summary: The study identified two distinct populations of stem cells that contribute to cementoblast differentiation at different times. CD90+ and Axin2+ cells become the main source of cementoblasts during adult homeostasis and following experimental induction of periodontal disease. Therefore, therapeutic approaches targeting these stem cells need to be tailored to the specific time period.
Article
Multidisciplinary Sciences
Ahmet Acar, Ana Hidalgo-Sastre, Michael K. Leverentz, Christopher G. Mills, Simon Woodcock, Martin Baron, Giovanna M. Collu, Keith Brennan
Summary: Notch and Wnt are two essential signaling pathways in animals, with typically opposing effects on cell fate decisions. Notch can limit Wnt signaling through mechanisms at the cell membrane and in the cell nucleus, contributing to the robustness of cell fate decisions by sharpening the distinction between opposing Notch/Wnt responses.
SCIENTIFIC REPORTS
(2021)
Article
Physiology
Kaixin Wangzhou, Zhiying Lai, Zishao Lu, Wanren Fu, Cheng Liu, Zhengeng Liang, Yi Tan, Conghui Li, Chunbo Hao
Summary: This study identified that miR-143-3p inhibits osteogenic differentiation of hPDLCs by targeting KLF5 and inactivating the Wnt/beta-catenin pathway.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Yingzhe Hu, Zhiguo Wang, Chun Fan, Pengyu Gao, Wenxuan Wang, Yutong Xie, Quanchen Xu
Summary: This study aimed to investigate the immunomodulatory effect of GMSC-Exo on periodontal bone regeneration and its role in regulating the NF-kappa B and Wnt/beta-catenin pathways in the periodontal inflammatory microenvironment. The results showed that GMSC-Exo could promote the expression of osteogenic differentiation-related factors in PDLSCs and inhibit the activation of the NF-kappa B pathway induced by P.g-LPS, leading to up-regulation of the Wnt/beta-catenin pathway. Blocking the Wnt/beta-catenin signaling abolished the inhibitory effect of GMSC-Exo on the NF-kappa B pathway. Therefore, GMSC-Exo has the ability to promote osteogenic differentiation and exhibit cross-regulation between the NF-kappa B and Wnt/beta-catenin pathways in the context of periodontal inflammation.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
C. Nottmeier, N. Liao, A. Simon, M. G. Decker, J. Luther, M. Schweizer, T. Yorgan, M. Kaucka, E. Bockamp, B. Kahl-Nieke, M. Amling, T. Schinke, J. Petersen, T. Koehne
Summary: The WNT/beta-catenin signaling pathway is crucial in periodontium biology, with specific extracellular WNT ligands like WNT1 demonstrating a strong promoting effect on cementum and alveolar bone formation in vivo. Experimental results indicate that Wnt1 promotes proliferation but not differentiation of cementoblasts.
JOURNAL OF DENTAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Roland Kotolloshi, Mieczyslaw Gajda, Marc-Oliver Grimm, Daniel Steinbach
Summary: The study revealed that BCL9L and Wnt/beta-catenin signaling play an oncogenic role in bladder cancer cells, and may be associated with the progression of bladder cancer. Mutations in UTRs of BCL9L can affect its activity, thereby influencing cell proliferation, migration, and invasion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biology
Liuyu Ru, Bowen Pan, Jiwei Zheng
Summary: This article reviews the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) in an inflammatory environment, focusing on the signaling pathways involved, and provides new ideas for the regenerative treatment of periodontal tissues.
OPEN LIFE SCIENCES
(2023)
Review
Engineering, Biomedical
Pingping Han, Guillermo A. Gomez, Georg N. Duda, Saso Ivanovski, Patrina S. P. Poh
Summary: The dynamics of cell mechanics and epigenetic signatures play a crucial role in cell behavior and fate, affecting the outcomes of tissue regeneration. While 2D geometric substrates have been widely used to study cell mechanics in response to the extracellular microenvironment, there is limited knowledge about cell mechanobiology and epigenetics in 3D biomaterial matrices. This review explores the potential of additive manufacturing for incorporating multi length-scale geometry features on scaffolds, and discusses how scaffold geometry influences cell and nuclear mechanosensing and epigenetic modifications, ultimately impacting tissue regeneration outcomes.
ACTA BIOMATERIALIA
(2023)
Article
Cell & Tissue Engineering
Chun Liu, Reuben Staples, Maria Natividad Gomez-Cerezo, Saso Ivanovski, Pingping Han
Summary: The COVID-19 pandemic has emphasized the need for point-of-care antibody tests for monitoring immune response. This requires 3D-printed devices, mobile health, and noninvasive sampling. 3D printing allows precise control over device features, while mobile health facilitates contact tracing and telehealth consultations. Saliva, as a noninvasive diagnostic method, is important in COVID-19 surveillance. Further research is needed on 3D-printed antibody tests and mobile health using noninvasive saliva.
TISSUE ENGINEERING PART C-METHODS
(2023)
Editorial Material
Engineering, Biomedical
Pingping Han, Saso Ivanovski
Summary: Harnessing three-dimensional bioprinted extracellular vesicles (EVs) holds great promise for advancing tissue engineering and regenerative medicine. This perspective explores the applications of 3D bioprinted EVs in tissue engineering, targeting angiogenesis, osteogenesis, chondrogenesis, myogenesis, and carcinoprevention. The technical challenges and future trends for bioprinted EVs in biofabrication and tissue engineering are examined, and a personalized bioprinted EVs concept and workflow for clinical translation are proposed.
Article
Materials Science, Multidisciplinary
Yan Wu, Meng Su, Shihao Zhang, Lan Xiao, Yin Xiao, Mengya Zhang, Ying Bei, Meiyun Li, Fan Zhang, Qi Yuan, Shiyong Wu, Jianyong Liu, Haitao Li, Xiaohuan Yuan, Meng Li, Yulin Li, Jie Gao
Summary: By reinforcing gelatin hydrogels with bovine serum albumin (BSA)-bridged bone marrow mesenchymal stem cell (BMSC)-derived nanovesicles (NV), the mechanical properties of the hydrogels were improved, making them suitable for diabetic wound healing.
MATERIALS & DESIGN
(2023)
Article
Cell & Tissue Engineering
Zhengmao Li, Qing Zhang, Shuang Yang, Yuncang Li, Andrej Atrens, Jagat Rakesh Kanwar, Wen Zhong, Bingpeng Lin, Cuie Wen, Yinghong Zhou, Yin Xiao
Summary: An optimized procedure for calibrating mu CT parameters using histological data was developed to reduce the impact of metal artifacts on data analysis. The application of optimized parameters resulted in more accurate 3D images and realistic statistical data. This method can effectively reduce the influence of metal artifacts on data analysis to some extent.
TISSUE ENGINEERING PART C-METHODS
(2023)
Article
Engineering, Biomedical
Chen Qin, Hongjian Zhang, Lei Chen, Meng Zhang, Jingge Ma, Hui Zhuang, Zhiguang Huan, Yin Xiao, Chengtie Wu
Summary: To achieve regeneration of highly vascularized and innervated tissues, a pre-angiogenic cell-laden scaffold with durable angiogenic functions is prepared. The scaffold supports long-term cell survival and growth, and exhibits durable angiogenic capability both in vitro and in vivo. The pre-angiogenic scaffolds can induce the neurogenetic differentiation and osteogenic differentiation, and may represent the future direction of biomaterials for complex tissue/organ regeneration.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Orthopedics
Xiaoxin Wu, Antonia RuJia Sun, Ross Crawford, Yin Xiao, Yanping Wang, Indira Prasadam, Xinzhan Mao
Summary: Chronic inflammation plays a crucial role in osteoarthritis (OA) pathology, but its impact on OA disease progression remains unclear. This study found that leukotriene B4 (LTB4) and its upstream enzyme, Leukotriene A(4) hydrolase (LTA(4)H), were highly expressed in OA cartilage. Inhibition of LTA(4)H showed therapeutic potential by suppressing cartilage degeneration and promoting regeneration, suggesting LTA(4)H as a potential therapeutic target for OA.
Review
Chemistry, Multidisciplinary
Jingyi Wen, Donglin Cai, Wendong Gao, Ruiying He, Yulin Li, Yinghong Zhou, Travis Klein, Lan Xiao, Yin Xiao
Summary: Treatment of large bone fractures is challenging, but osteoimmunology has been used to develop biomaterials for advanced bone regeneration. Nanoparticle-based drug delivery systems enable controlled release and targeted delivery, benefiting bone regeneration through osteoimmunomodulation. This review discusses the significance of osteoimmunology, the advancement of NP-based approaches, and the application of NPs in macrophage-targeting drug delivery for advanced osteoimmunomodulation.
Article
Chemistry, Multidisciplinary
Lin Du, Chen Qin, Hongjian Zhang, Fei Han, Jianmin Xue, Yufeng Wang, Jinfu Wu, Yin Xiao, Zhiguang Huan, Chengtie Wu
Summary: A biomimetic ink mimicking the tendon/bone tissues was developed using tendon/bone-related cells and Mo-containing silicate (MS) bioceramics. Biomimetic multicellular scaffolds were then fabricated via 3D bioprinting to simulate the hierarchical structure and cellular composition of tendon-to-bone interfaces. The study is significant for inducing the regeneration of complex structures in the interface region.
Article
Dentistry, Oral Surgery & Medicine
Pingping Han, Nigel Johnson, Abdalla Abdal-hay, Corey S. S. Moran, Carlos Salomon, Saso Ivanovski
Summary: This study aimed to enrich and compare three types of extracellular vesicles (apoptotic bodies, microvesicles, and small EV) from three periodontal cells (PDLCs, OBs, and GFs), and evaluate their uptake and effects on BFP-MSCs. The results showed that the EVs from the periodontal cells promoted proliferation, migration, and expression of osteogenic genes in the BFP-MSCs.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Article
Engineering, Biomedical
Hai-Feng Liang, Yan-Pei Zou, An-Nan Hu, Ben Wang, Juan Li, Lei Huang, Wei-Sin Chen, Di-Han Su, Lan Xiao, Yin Xiao, Yi-Qun Ma, Xi-Lei Li, Li-Bo Jiang, Jian Dong
Summary: This study developed a biomimetic magnetic scaffold to mimic the structure and composition of bone using magnetic nanoparticles and specific protein structures. The results showed that the scaffold, when stimulated by a static magnetic field, promoted cell migration and osteogenic differentiation, and also facilitated new bone formation in animal experiments. The study also revealed the molecular mechanisms underlying bone formation, which involves the release of calcium ions from the endoplasmic reticulum.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Microbiology
Srinivas Sulugodu Ramachandra, Patricia Wright, Pingping Han, Abdalla Abdal-hay, Ryan Lee, Saso Ivanovski
Summary: Oral biofilms are complex structures that cause dental diseases and have been extensively studied using various models and techniques. This narrative review provides a summary of existing literature on biofilm models and assessment techniques, serving as a guide for beginners and a refresher for experienced researchers. The review discusses advantages and limitations of 2D and 3D models, focuses on the growth of biofilms on melt electrowritten fibrous scaffolds, and summarizes studies on oral microcosm models. It also analyzes the advantages and limitations of biofilm assessment methods, including culture, microscopy, fluorescence in situ hybridization, and sequencing.
Review
Chemistry, Multidisciplinary
Rijia Lin, Milton Chai, Yinghong Zhou, Vicki Chen, Thomas D. Bennett, Jingwei Hou
Summary: The melting phenomenon in metal-organic frameworks (MOFs) is recognized as a typical characteristic of the fourth generation MOFs. Molten MOFs can be processed into mechanically robust glassy MOF macrostructures and can also be combined with other functional materials, such as crystalline MOFs, inorganic glass, and metal halide perovskites, to achieve highly tunable interfacial characteristics. MOF glass composites have emerged as a family of functional materials with dynamic properties and hierarchical structural control, enabling the fabrication of advanced separation, catalysis, optical, and biomedical devices. In this review, we discuss the approaches for designing, fabricating, and characterizing MOF glass composites, and identify key application opportunities and remaining challenges in terms of thermal and chemical compatibility, interfacial properties, and scalability.
CHEMICAL SOCIETY REVIEWS
(2023)
Article
Pharmacology & Pharmacy
Tingyu Jin, Ziwen Zhang, Yin Xiao, Xuerong Geng, Xingyi Zhu
Summary: PZ-malic acid cocrystal was prepared using liquid assisted grinding method to improve the poor water solubility and light instability of PZ. The cocrystal showed improved solubility in water and simulated intestinal environment, as well as good light and thermal stability. However, phase separation of the cocrystal in water required the addition of a suitable amount of polymer.
LATIN AMERICAN JOURNAL OF PHARMACY
(2023)
Article
Materials Science, Multidisciplinary
Jiajie Chen, Yinghui Wang, Chengtie Wu, Yin Xiao, Yufang Zhu
Summary: This study developed a dendritic mesoporous silica nanoparticle decorated with SARS-CoV-2 spike protein for targeted delivery of RNA-dependent RNA polymerase-specific siRNA to combat SARS-CoV-2 infection. The results demonstrated improved silencing effect on the transfected SARS-CoV-2 gene and suggested the potential of this nanosystem as a promising treatment option for SARS-CoV-2 infection.
APPLIED MATERIALS TODAY
(2023)
Article
Materials Science, Biomaterials
Izabela Stupka, Artur P. Biela, Bernard Piette, Agnieszka Kowalczyk, Karolina Majsterkiewicz, Kinga Borzecka-Solarz, Antonina Naskalska, Jonathan G. Heddle
Summary: Artificial protein cages, such as TRAP-cages, have potential applications in vaccines and drug delivery. TRAP-cages have the ability to control the disassembly conditions by modifying the interface between their building blocks. By using TRAP rings with different numbers of monomers, it is possible to predict the formation of other cages.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Guo Zhang, Yu Wang, Hua Qiu, Lei Lu
Summary: This study presents a one-pot synthesis method for flower-like AMPs@EAMP particles by combining antimicrobial peptides with ellagic acid, offering enlarged surface area, excellent biocompatibility, and broad-spectrum antibacterial activity. In vivo studies indicate their potential for tissue repair and immune barrier reconstruction.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Jiamei Zhang, Lingshuang Wang, Cheng Xu, Yingui Cao, Shengsheng Liu, Rui L. Reis, Subhas C. Kundu, Xiao Yang, Bo Xiao, Lian Duan
Summary: Pluronic F127 modified silk fibroin film with different types of antibacterial agents could accelerate wound recovery by promoting fibroblast adhesion, eradicating bacteria, and facilitating angiogenesis and re-epithelialization.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Yinsheng Liu, Mingyue Wang, Yinfei Hui, Lei Sun, Yanrui Hao, Henlong Ren, Hao Guo, Wu Yang
Summary: In this study, a rare-earth hybrid luminescent material was developed for the detection of a biomarker for anthrax. The material showed excellent selectivity and high sensitivity, allowing for the determination of the biomarker in saliva and urine. Additionally, a convenient point-of-care testing method using fluorescent test paper and a smartphone was established for the initial diagnosis of anthrax.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Review
Materials Science, Biomaterials
Wenshuai Yang, Jingsi Chen, Ziqian Zhao, Meng Wu, Lu Gong, Yimei Sun, Charley Huang, Bin Yan, Hongbo Zeng
Summary: Injectable hydrogels with shear-thinning and/or in situ formation properties offer distinct advantages in bioengineering applications, as they can be directly delivered to target sites, possess self-healing abilities, and simplify the implantation process.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)