Article
Immunology
Alhumaidi B. Alabbas
Summary: This study developed a multi-epitope-based vaccine against Sin Nombre orthohantavirus using computational methods. The selected epitopes showed potential as antigenic, nonallergenic, nontoxic, and water-soluble components of the vaccine. Molecular docking and simulation analysis demonstrated that the vaccine had good binding affinity with TLR-4 and TLR-8, which are important for inducing an immune response. However, experimental validation is needed to confirm these findings.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
F. A. Dain Md Opo, Mohammed Moulay, Ali Zari, Afnan Alqaderi, Saleh Alkarim, Talal Zari, Mohiuddin Ahmed Bhuiyan, Maged Mostafa Mahmoud, Fadwa Aljoud, Mohd Suhail, Sherif Edris, Wafaa S. Ramadan, Mohammad Amjad Kamal, Said Nemmiche, Foysal Ahammad
Summary: Alterations in the EGFR gene have been linked to various cancers, and targeted drugs are becoming less effective. However, this study identified four compounds with improved effects and lower toxicity.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Immunology
Gail L. Rodgers, Cynthia G. Whitney, Keith P. Klugman
Summary: Pneumococcal conjugate vaccine has successfully reduced the burden of disease and provided herd protection for those who cannot be vaccinated. While widely rolled out in poor countries, there are still many unvaccinated children in middle income countries. Solutions being considered include reducing doses and supporting more affordable vaccine sources.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Review
Immunology
Takeshi Saito, Rachel A. A. Reyna, Satoshi Taniguchi, Kirsten Littlefield, Slobodan Paessler, Junki Maruyama
Summary: The Arenaviridae family includes viruses that cause severe and often fatal diseases in humans. High-containment facilities are required to handle these highly pathogenic viruses. The development of vaccines is essential for combating arenavirus infections, but currently there are limited options available. We provide a summary of the recent updates on vaccine candidates against these viruses.
Article
Biotechnology & Applied Microbiology
Xiangkuan Zheng, Sixiang Xu, Zhuohao Wang, Xingyu Tao, Yuqing Liu, Lei Dai, Yubao Li, Wei Zhang
Summary: This study used pan-genome binding reverse vaccinology to identify potential cross-protective antigens against R. anatipestifer, laying the foundation for the development of a cross-protective subunit vaccine.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Guoxing Luo, Yuanjun Zeng, Han Yang, Yijian Li, Lianwei Yang, Cao Li, Feibo Song, Shiyin Zhang, Tingdong Li, Shengxiang Ge, Jun Zhang, Ningshao Xia
Summary: Truncated VP4* protein has the potential to be a viable rotavirus vaccine candidate, as it can stimulate high levels of neutralizing antibodies. A bivalent VP4*-based vaccine can provide protection against different genotypes of rotavirus, similar to trivalent vaccines.
Review
Immunology
Anke Osterloh
Summary: Bacterial infections are a major health problem worldwide, with increasing difficulty in treatment due to antibiotic resistance. Prophylactic vaccines are urgently needed to combat bacterial pathogens. However, developing vaccines against bacteria, especially intracellular bacteria, is challenging and requires alternative antigen delivery strategies.
Article
Immunology
Su Min Pack, Peter J. Peters
Summary: The production of SARS-CoV-2 vaccines and the use of structural vaccinology are discussed in this article. Cryo-electron microscopy plays a crucial role in vaccine development by providing three-dimensional structures of the virus and enabling the design of new candidates to combat emerging mutants. The development of vaccines to halt the COVID-19 pandemic is a milestone for the design and manufacturing of pan-coronavirus vaccines.
Article
Veterinary Sciences
Zhuohao Wang, Xiangkuan Zheng, Genglin Guo, Yongyi Dong, Zhengjun Xu, Xiankai Wei, Xiangan Han, Yuqing Liu, Wei Zhang
Summary: This study investigated avian pathogenic Escherichia coli (APEC) using genomic analysis and identified four potential vaccine candidates using a reverse vaccinology method. The immunoreactivity of these candidates was verified, suggesting their potential in developing an effective vaccine against APEC infection. This in silico analysis method may facilitate rapid screening of vaccine candidates and contribute to the control of APEC infection.
Article
Microbiology
Jie Li, Jingxuan Qiu, Zhiqiang Huang, Tao Liu, Jing Pan, Qi Zhang, Qing Liu
Summary: Salmonella is a major foodborne pathogen, and vaccination is the most effective means to combat its infections. In this study, reverse vaccinology was used to identify vaccine candidates with high immunogenicity and protective immunity. The results showed that some proteins screened in the study could serve as good candidates for broad-spectrum vaccines.
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Sama Rashidi, Seyed Nooreddin Faraji, Amirreza Javadi Mamaghani, Saeid Hatam, Bahram Kazemi, Peyman Bemani, Seyyed Javad Seyyed Tabaei, Gholamreza Hatam
Summary: Leishmaniasis, especially Cutaneous Leishmania (CL), is a major infectious disease worldwide. This study aims to design a new multi-epitope DNA vaccine using immune bioinformatics to effectively control the disease. Multiple antigens were selected and their MHC-I epitopes were used to construct the vaccine. The designed vaccine showed high stability and antigenicity, making it a suitable model for further animal and human studies.
SCIENTIFIC REPORTS
(2022)
Article
Biology
Anjali Lathwal, Rajesh Kumar, Gajendra P. S. Raghava
Summary: This study developed subunit vaccine candidates against lung cancer-causing oncogenic viruses using a reverse vaccinology approach. Through systematic analysis of protein components from nine oncogenic virus species, 125 best antigenic epitopes were identified with predicted B-cell, T-cell, and/or MHC-binding capability, as well as vaccine adjuvant potential. These epitopes show promising immunogenic potential for future development as vaccines against lung cancer-causing viruses.
COMPUTERS IN BIOLOGY AND MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Ting Zhang, Ningchen Zheng, Zhirong Wang, Xuemei Xu
Summary: The multimeric form of SARS-CoV-2 S protein receptor-binding domain (RBD) shows better immunogenicity and induces higher levels of neutralizing antibody responses. A recombinant protein, 2RBDpLC, was constructed by fusing RBD single-chain dimer with a trimerization motif and introducing a cysteine at the C-terminus. It forms RBD dodecamers through trimerization motif and intermolecular disulfide bonds, and exhibits promising potential as a vaccine candidate with the ability to generate cross-neutralizing antibodies against Delta and Omicron variants.
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2023)
Article
Infectious Diseases
Ashkan Bagheri, Navid Nezafat, Mahboobeh Eslami, Younes Ghasemi, Manica Negahdaripour
Summary: In this study, a therapeutic and prophylactic vaccine against HPV16 was designed using immunoinformatic and computational tools. The vaccine construct contained immunodominant epitope regions from L2, E6, and E7 proteins of HPV16, along with TLR4 agonists as vaccine adjuvants. The designed constructs showed stable ligand-receptor interactions with TLR4 in molecular docking simulations, demonstrating potential for both prophylactic and therapeutic applications against HPV.
INFECTION GENETICS AND EVOLUTION
(2021)
Article
Biology
Kamal Rawal, Robin Sinha, Swarsat Kaushik Nath, P. Preeti, Priya Kumari, Srijanee Gupta, Trapti Sharma, Ulrich Strych, Peter Hotez, Maria Elena Bottazzi
Summary: The article presents Vaxi-DL, a web-based deep learning software that evaluates the potential of protein sequences as vaccine target antigens. The software accurately predicts target antigens in bacteria, protozoa, fungi, and viruses that cause infectious diseases in humans. The tool has demonstrated a high sensitivity, making it useful for prioritizing potential vaccine candidates.
COMPUTERS IN BIOLOGY AND MEDICINE
(2022)
Article
Biotechnology & Applied Microbiology
Monika Yadav, Dibyabhaba Pradhan, Rana P. Singh
Summary: This study identified hub genes associated with oral squamous cell carcinoma, which play key roles in cellular motility, invasion, and adhesion processes. Additionally, enrichment of DEGs in pathways such as PI3K-Akt signaling, focal adhesion, and regulation of actin cytoskeleton was observed.
Article
Biochemistry & Molecular Biology
Jangampalli Adi Pradeepkiran, Manne Munikumar, Arubala P. Reddy, P. Hemachandra Reddy
Summary: The study aimed to understand the protective effects of small molecule ligands for phosphorylated tau in AD progression. Small molecule ligands binding to phosphorylated tau can reduce tau hyperphosphorylation levels in AD and promote mitochondrial biogenesis and synaptic activities while decreasing mitochondrial fission.
HUMAN MOLECULAR GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Sudheer Kumar Katari, Chiranjeevi Pasala, Ravina Madhulitha Nalamolu, Aparna R. Bitla, Amineni Umamaheswari
Summary: This study focused on designing rational drugs against the hyperactivated MMP-7 in humans, which plays a crucial role in the inflammatory and metastatic processes of various carcinomas. Through structure-based drug design and molecular dynamics simulations, four novel and potent MMP-7 binders were identified, showing promising pharmacological features and inhibitory effects on ECM proteolysis in cancerous conditions.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biotechnology & Applied Microbiology
Aishwarya Kochhar, Noor Saba Khan, Ravi Deval, Dibyabhaba Pradhan, Lingaraja Jena, Rajabrata Bhuyan, Tanmaya Kumar Sahu, Arun Kumar Jain
Summary: Protein-protein interactions of IL17 are crucial in autoimmune and inflammatory diseases, and blocking these interactions through peptide inhibitors can be cost-effective and orally available compared to biologics. The crystal structure of IL17A in complex with a high affinity peptide inhibitor was investigated to identify hot spots for improving potency.
Review
Infectious Diseases
Seshadri Reddy Varikasuvu, Balachandar Thangappazham, Alekya Vykunta, Pragathi Duggina, Munikumar Manne, Hemanth Raj, Sowjanya Aloori
Summary: This article is a meta-analysis of randomized controlled trials on the use of vitamin D intervention in COVID-19 outcomes. By including 6 RCTs involving 551 COVID-19 patients, the study found that vitamin D supplementation can reduce the severity, mortality, and RT-PCR positivity rates in COVID-19 patients.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Rashi Verma, Dibyabhaba Pradhan, Arnab Nayek, Harpreet Singh, Arun Kumar Jain, Luqman Ahmad Khan
Summary: The study identified Arbutamine, Hydroxychloroquine, and Carindacillin as drugs with better binding affinity to TRR1, TOM40, and YHB1, respectively. The Arbutamine-TRR1 complex was considered the best drug-target complex and showed effectiveness against multiple Candida species.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Review
Infectious Diseases
Rashi Verma, Dibyabhaba Pradhan, Ziaul Hasan, Harpreet Singh, Arun Kumar Jain, Luqman Ahmad Khan
Summary: This study presents the distribution and antifungal resistance patterns of Candida species among the Indian population, which could assist in forming treatment recommendations for the region and beyond in the future.
Article
Immunology
Ankita Ray, Tanu Bhati, Renu Arora, Dibyabhaba Pradhan, Suhel Parvez, Sangita Rastogi
Summary: Research on the mechanisms of Chlamydia trachomatis-induced recurrent spontaneous abortion is still in its early stages, requiring the development of prevention strategies. It is hypothesized that microRNAs play a crucial role in regulating recurrent spontaneous abortion during infection. miR-16 and miR-559 may serve as potential biomarkers for recurrent spontaneous abortion in infected women.
MICROBIAL PATHOGENESIS
(2021)
Article
Multidisciplinary Sciences
Seshadri Reddy Varikasuvu, Saurabh Varshney, Naveen Dutt, Manne Munikumar, Shahir Asfahan, Paresh P. Kulkarni, Pratima Gupta
Summary: This study conducted a systematic review and meta-analysis to quantify the associations of D-dimer with disease progression in COVID-19 patients. The findings suggest a significant correlation between higher D-dimer levels and the risk of overall disease progression, indicating its potential as a prognostic marker. Rapid assessment of D-dimer is recommended for predicting adverse outcomes in COVID-19 patients.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Ramakrishna Ungarala, Manne Munikumar, Sukesh Narayan Sinha, Dileshwar Kumar, R. Shyam Sunder, Suresh Challa
Summary: This study evaluated the therapeutic effects of oxidised Epigallocatechin Gallate (O-EGCG) compared to native EGCG, finding improved antioxidant capacity and anti-inflammatory properties. The O-EGCG showed significant inhibitory action against SARS-CoV-2, suggesting its potential as a treatment for COVID-19. Further in vivo studies and human clinical trials are needed to understand the mechanism of action and confirm its efficacy.
Article
Integrative & Complementary Medicine
Thrigulla Saketh Ram, Manne Munikumar, Vankudavath Naik Raju, Parasannanavar Devaraj, Naveen Kumar Boiroju, Rajkumar Hemalatha, P. V. V. Prasad, Manohar Gundeti, Brijesh S. Sisodia, Sharad Pawar, G. P. Prasad, Mukesh Chincholikar, Sumeet Goel, Anupam Mangal, Sudesh Gaidhani, N. Srikanth, K. S. Dhiman
Summary: The study evaluated the compounds in AYUSH-64 drug against SARS-CoV-2, with findings showing 35 compounds had good binding energies and Akuammicine N-Oxide was the most effective compound with strong affinity and interactions. These results suggest that AYUSH-64 could be a potential candidate for managing COVID-19.
JOURNAL OF AYURVEDA AND INTEGRATIVE MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Ranjitha Acharya, Shilpa S. Shetty, Gollapalli Pavan, Flama Monteiro, Manne Munikumar, Sriram Naresh, Nalilu Suchetha Kumari
Summary: Fatty acid transport protein 1 (FATP1) is a crucial protein involved in regulating signaling pathways and playing a key role in various physiological and pathological conditions. Understanding its structure is essential for understanding its function and developing effective treatments.
Review
Chemistry, Analytical
Noor Saba Khan, Dibyabhaba Pradhan, Saumya Choudhary, Pallavi Saxena, Nitesh Kumar Poddar, Arun Kumar Jain
Summary: Chlorpyrifos is a widely used organophosphate pesticide, but concerns about its environmental contamination and impact on human health have raised alarm. Detection of CPF in blood samples is crucial to prevent severe health consequences from continuous exposure.
JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Anil Kumar Singh, Sudheer Kumar Katari, Amineni Umamaheswari, Abhay Raj
Summary: This study evaluated the binding affinity of bacterial-derived lignin peroxidase with selected lignin model compounds and validated such docked complexes through molecular dynamics simulations. The findings demonstrate that bacterial LiP can effectively catalyze multiple lignin model compounds, and it might further be used as an effective tool for sustainable mitigation of diverse environmental contaminants.
Article
Medicine, Research & Experimental
Saumya Choudhary, Rishika Anand, Dibyabhaba Pradhan, Banajit Bastia, Shashi Nandar Kumar, Harpreet Singh, Poonam Puri, George Thomas, Arun Kumar Jain
Summary: This study utilized bioinformatics to analyze gene expression data of mild and severe psoriasis patients, revealing numerous differentially expressed genes and identifying important hub genes. The results indicate significant differences in gene expression regulation and signaling pathways between patients with different disease severity levels of psoriasis.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)