Article
Food Science & Technology
Didem Sutay Kocabas, John Lyne, Zeynep Ustunol
Summary: Hydrolytic enzymes play a crucial role in the dairy industry, impacting the sensory qualities of dairy products such as texture, taste, and aroma. Microbial hydrolases are favored over plant and animal derived alternatives. There is a growing demand for hydrolytic enzymes with superior properties to enhance the quality of dairy products.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mahadev Latha, Achintya Kumar Dolui, Panneerselvam Vijayaraj
Summary: A seed-specific lipase, SL-AT4G28520, was identified to have monoacylglycerol lipase activity during germination, while the canonical FL-AT4G28520 did not show detectable activity. This study suggests that the activity of SL-AT4G28520 lipase is dependent on physiological conditions and provides insights into lipid mobilization during germination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Polymer Science
Amber N. Johnson, Daniel E. Barlow, Abigail L. Kelly, Vanessa A. Varaljay, Wendy J. Crookes-Goodson, Justin C. Biffinger
Summary: Natural and synthetic polymers degrade over time due to changes in bonding, with microorganisms using chemical processes to release carbon sources. Polyesters and polyurethanes, making up 35% of global polymer production, are susceptible to degradation by enzymes released by microorganisms. This review focuses on recent research on biodegradation of these polymers.
POLYMER INTERNATIONAL
(2021)
Article
Chemistry, Physical
Qinyu Li, Xiaojia Chen, Xiangcen Liu, Zheng Chen, Yang Han, Peng Zhou, Jiping Shi, Zhijun Zhao
Summary: The esterase PTCL1-EstA from Paenarthrobacter aurescens TC1 was successfully expressed and purified. Homology modeling and substrate docking predicted the active site residues in PTCL1-EstA and its enzymatic activity and stability under different conditions were analyzed. Fermentation medium optimization increased the enzyme activity of PTCL1-EstA, showcasing its potential for industrial production.
Article
Microbiology
Yong-Jik Lee, Dariimaa Ganbat, DoKyung Oh, HyeWon Kim, Ga Eul Jeong, In-Tae Cha, Seong-Bo Kim, Gaewon Nam, You-Jung Jung, Sang-Jae Lee
Summary: This study identified 29 thermophilic bacterial isolates from hot springs in Korea and investigated their extracellular hydrolase activity. Geobacillus species were found to be the most abundant and showed promising potential for industrial applications.
Article
Microbiology
Robert Ruginescu, Madalin Enache, Octavian Popescu, Ioana Gomoiu, Roxana Cojoc, Costin Batrinescu-Moteau, Gabriel Maria, Maria Dumbravician, Simona Neagu
Summary: This study evaluated the enzyme activities of some halotolerant strains and characterized their functional parameters. Bacillus strains showed the highest catalytic activities. The selected Bacillus strains produced enzymes that were active over wide ranges of salinity, temperature, and pH.
Article
Biotechnology & Applied Microbiology
Shenao Zhang, Feilong Chen, Juntao Ke, Yuling Hao, Ruru Pan, Tao Hong, Yongpei Dai, Shaoxing Chen
Summary: Extracellular proteases of haloarchaea have the ability to adapt to high salt concentrations and can be utilized in industrial or biotechnology applications under hypersaline conditions. However, the diversity of these proteases is largely unknown despite the availability of many sequenced genomes. In this study, the gene encoding the extracellular protease Hly176B from Haloarchaeobius sp. FL176 was cloned and expressed in Escherichia coli, revealing its catalytic triad and unique properties. This research enhances our understanding of extracellular proteases and broadens their potential industrial uses.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Review
Medicine, Research & Experimental
Dengke Tian, Yuzhi Liu, Chengyuan Liang, Liang Xin, Xiaolin Xie, Dezhu Zhang, Minge Wan, Han Li, Xueqi Fu, Hong Liu, Wenqiang Cao
Summary: The SARS-CoV-2 outbreak since late 2019 has presented a global health challenge, but multiple therapeutic drugs have been developed to combat it. Host-targeted drugs inhibiting TMPRSS2 prevent virus entry into cells, while small molecule therapeutics targeting RNA polymerase and proteases play crucial roles in treatment.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Microbiology
Robert W. B. Brown, Aabha Sharma, Miguel Rey Villanueva, Xiaomo Li, Ouma Onguka, Leeor Zilbermintz, Helen Nguyen, Ben A. Falk, Cheryl L. Olson, Joann M. Taylor, Conrad L. Epting, Rahul S. Kathayat, Neri Amara, Bryan C. Dickinson, Matthew Bogyo, David M. Engman
Summary: Dynamic post-translational modifications in eukaryotic cells allow rapid and specific regulation of protein functions. The S-acylation process involves reversible lipid modification of proteins, while depalmitoylation is necessary for acylation homeostasis. The identification of a homologue of human APT1 in Trypanosoma brucei, named TbAPT-L, suggests a lipase function rather than depalmitoylase activity, with modulation of TbAPT-L showing no effect on parasite growth or cellular depalmitoylation activity in vitro.
Article
Pharmacology & Pharmacy
Pei-Chen Yu, Chen-Hao Huang, Chih-Jung Kuo, Po-Huang Liang, Lily Hui-Ching Wang, Max Yu-Chen Pan, Sui-Yuan Chang, Tai-Ling Chao, Si-Man Ieong, Jun-Tung Fang, Hsuan-Cheng Huang, Hsueh-Fen Juan
Summary: This study utilized bioinformatics to screen compounds with therapeutic potential for COVID-19 and evaluated their inhibitory effect and binding capability. The results revealed that tamoxifen possesses anti-SARS-CoV-2 property, which may accelerate drug screening for COVID-19 and other diseases.
Article
Biochemistry & Molecular Biology
Isobel E. Bowles, Emily H. Pool, Benjamin S. Lancaster, Emily K. Lawson, Christopher P. Savas, Zach J. Kartje, Luke Severinac, David H. Cho, Mark R. Macbeth, R. Jeremy Johnson, Geoffrey C. Hoops
Summary: The study explored the inhibition of enzymatic activity of Mycobacterium tuberculosis by divalent metals, showing specific divalent transition metal cations strongly inhibit its enzymatic activity. Structural analysis and targeted mutagenesis revealed the involvement of multiple chelating residues on a flexible loop in the dynamic allosteric regulation of Rv0045c by divalent metals. This is the first structural confirmation of allosteric regulation of enzymatic activity in the large and diverse enzyme superfamily of serine hydrolases like Rv0045c.
Article
Biochemistry & Molecular Biology
Timothy Lamantia, Amanda Jansch, Justin D. Marsee, Mitch H. Weiland, Justin M. Miller
Summary: In recent years, there has been a focus on creating and using new biodegradable polymeric materials. This study investigates the properties of a dimeric enzyme called PahZ1KT-1 and its role in catalyzing the degradation of poly(aspartic acid), a biodegradable substitute for non-biodegradable polycarboxylates. The researchers found that both monomers and dimers of PahZ1KT-1 have similar catalytic rates for poly(aspartic acid) degradation, but the activation energy for monomers is lower than for dimers.
BIOPHYSICAL CHEMISTRY
(2022)
Article
Virology
Maximilian Grosse, Christian Setz, Pia Rauch, Janina Auth, Martina Morokutti-Kurz, Vladimir Temchura, Ulrich Schubert
Summary: This study found that certain DUB inhibitors can interfere with the replication of SARS-CoV-2. These inhibitors are able to block the activity of the viral protease, thereby restricting virus replication. These findings suggest that DUBs could be potential therapeutic targets.
Article
Chemistry, Medicinal
Ittipat Meewan, Jacob Kattoula, Julius Y. Kattoula, Danielle Skinner, Pavla Fajtova, Miriam A. Giardini, Brendon Woodworth, James H. McKerrow, Jair Lage de Siqueira-Neto, Anthony J. O'Donoghue, Ruben Abagyan
Summary: Inhibitors containing thiuram disulfide or dithiobis-(thioformate) were found to be potential drugs against SARS-CoV-2. These compounds showed strong inhibitory effects in enzymatic and cell-based assays, suggesting their promise in the treatment of the COVID-19 virus or related variants.
Article
Biotechnology & Applied Microbiology
J. Furhan, J. Nissar
Summary: The psychrotolerant bacterium Serratia DLCP2, isolated from sediment of Dal Lake in India, secretes cold-adapted and alkali-tolerant protease with the highest production at 20 degrees C and pH 9.0. The purified enzyme, with a molecular weight of about 60 kDa, shows maximal activity at 15 degrees C and pH 10.0, and can remove protein stains from white cloth pieces efficiently.
APPLIED BIOCHEMISTRY AND MICROBIOLOGY
(2021)
Review
Biochemical Research Methods
Philippe V. Jutras, Isobel Dodds, Renier A. L. van der Hoorn
CURRENT OPINION IN BIOTECHNOLOGY
(2020)
Letter
Biochemistry & Molecular Biology
Elena A. Minina, Jens Staal, Vanina E. Alvarez, John A. Berges, Ilana Berman-Frank, Rudi Beyaert, Kay D. Bidle, Frederic Bornancin, Magali Casanova, Juan J. Cazzulo, Chang Jae Choi, Nuria S. Coll, Vishva M. Dixit, Marko Dolinar, Nicolas Fasel, Christiane Funk, Patrick Gallois, Kris Gevaert, Emilio Gutierrez-Beltran, Stephan Hailfinger, Marina Klemencic, Eugene V. Koonin, Daniel Krappmann, Anna Linusson, Mauricio F. M. Machado, Frank Madeo, Lynn A. Megeney, Panagiotis N. Moschou, Jeremy C. Mottram, Thomas Nystrom, Heinz D. Osiewacz, Christopher M. Overall, Kailash C. Pandey, Juergen Ruland, Guy S. Salvesen, Yigong Shi, Andrei Smertenko, Simon Stael, Jerry Stahlberg, Maria Fernanda Suarez, Margot Thome, Hannele Tuominen, Frank Van Breusegem, Renier A. L. van der Hoorn, Assaf Vardi, Boris Zhivotovsky, Eric Lam, Peter V. Bozhkov
Article
Biochemistry & Molecular Biology
Marcel Bach-Pages, Felix Homma, Jiorgos Kourelis, Farnusch Kaschani, Shabaz Mohammed, Markus Kaiser, Renier A. L. van der Hoorn, Alfredo Castello, Gail M. Preston
Review
Plant Sciences
Alice Godson, Renier A. L. van der Hoorn
Summary: Apoplastic proteases play crucial roles in plant defense, with most being induced upon infection and 17 genetically required for the immune response. While the proteolytic activity of these proteases has been confirmed, its necessity for biological function is not always clear.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Shuaishuai Wang, Rongkang Xing, Yan Wang, Haidong Shu, Shenggui Fu, Jie Huang, Judith K. Paulus, Mariana Schuster, Diane G. O. Saunders, Joe Win, Vivianne Vleeshouwers, Yuanchao Wang, Xiaobo Zheng, Renier A. L. van der Hoorn, Suomeng Dong
Summary: The study reveals the important function of plant apoplastic proteases in cleaving pathogen proteins to release immunogenic peptides. A newly identified protein PC2 induces immunity in plants only after being cleaved by apoplastic proteases, and a core peptide of PC2 carrying key cysteines is found to be sufficient for inducing cell death. Additionally, protease inhibitors produced by the pathogen dampen the host immunity elicited by PC2.
Article
Plant Sciences
Pierre Buscaill, Nattapong Sanguankiattichai, Yoon Joo Lee, Jiorgos Kourelis, Gail Preston, Renier A. L. van der Hoorn
Summary: The agromonas method is a simple, fast, and robust approach for studying the interactions between the model plant pathogen Pseudomonas syringae and plant hosts. It allows rapid structure-function analysis of immune components and testing of immune hydrolase function.
Editorial Material
Plant Sciences
Renier A. L. van der Hoorn, Marina Klemencic
Summary: Plant proteases play important yet mysterious roles in plant life, participating in various activities but their regulation and actions remain poorly understood. The irreversible action of proteases on proteins is tightly regulated, and their modes of cleaving multiple substrates are challenging to predict.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Philippe Jutras, Riccardo Soldan, Isobel Dodds, Mariana Schuster, Gail M. Preston, Renier A. L. van der Hoorn
Summary: A bioluminescent strain of Agrobacterium tumefaciens GV3101 was generated to monitor luminescence during agroinfiltration, providing a versatile and non-destructive imaging tool for studying plant responses to Agrobacterium infiltration.
Article
Plant Sciences
Daniela J. Sueldo, Alice Godson, Farnusch Kaschani, Daniel Krahn, Till Kessenbrock, Pierre Buscaill, Christopher J. Schofield, Markus Kaiser, Renier A. L. van Der Hoorn
Summary: This study reports the dynamics of extracellular hydrolases in Nicotiana benthamiana upon infection with Pseudomonas syringae. The activity of 82 hydrolases, mostly serine hydrolases, increases during infection, while the activity of 60 hydrolases, mostly glycosidases and cysteine proteases, is suppressed. The study also reveals the antibacterial activity of NbPR3 and its essential active site substitution.
Letter
Plant Sciences
Rawit Longsaward, Nattapong Sanguankiattichai, Unchera Viboonjun, Renier A. L. van der Hoorn
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Isobel Dodds, Changlong Chen, Pierre Buscaill, Renier A. L. van Der Hoorn
Summary: Nicotiana benthamiana is increasingly used for transient gene expression but the expression is low in fully developed plants. The perception of cold shock protein (CSP) is thought to be the cause. This study demonstrates that silencing NbCORE in older plants increases GFP fluorescence and protein production, allowing for more efficient protein expression.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Maria Barco-Antonanzas, Maria Font-Farre, Mikel V. Eceiza, Miriam Gil-Monreal, Renier A. L. van der Hoorn, Mercedes Royuela, Ana Zabalza
Summary: This study demonstrates that glyphosate and pyrithiobac have significant inhibitory effects on EPSPS and ALS enzymes. The protease activity profiles of different populations of Amaranthus palmeri in response to these herbicides were characterized. The results show that both herbicides induce increased cysteine protease activity and amino acid accumulation, indicating their role in protein degradation and increased free amino acids. Conversely, no changes in proteolytic activities were observed in populations with target-site resistance, suggesting this effect is specific to sensitive plants.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Plant Sciences
Maria Font Farre, Daniel Brown, Maurice Konig, Brian J. Killinger, Farnusch Kaschani, Markus Kaiser, Aaron T. Wright, Jonathan Burton, Renier A. L. van der Hoorn
Summary: This study presents an easy and quick assay for photoaffinity labeling of GSTs in different plant species. The results demonstrate that GSTs can be induced by various types of stress and this method is useful for studying GST induction in different plant species.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Felix Homma, Jie Huang, Renier A. L. van der Hoorn
Summary: This study used AlphaFold-Multimer to screen small secreted proteins (SSPs) from plant pathogens and identified non-annotated SSPs that interact with tomato defense enzymes. Four of these SSPs were confirmed as inhibitors of subtilase P69B, demonstrating the application of AI in predicting cross-kingdom protein interactions.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Pierre Buscaill, Renier A. L. van der Hoorn
Summary: The study highlights nine different extracellular strategies that microbes employ to avoid detection by the host, operating at three different levels. These strategies involve utilizing polymorphisms, downregulation of MAMP production, shielding MAMP precursors, and degrading or sequestering MAMPs before they are perceived by the host.