Article
Chemistry, Multidisciplinary
Gustavo Freitas Fachin, Thiago Revillion Dinato, Frederico Ballve Prates, Thomas Connert, Rina Andrea Pelegrine, Carlos Eduardo da Silveira Bueno
Summary: This in vitro study evaluated the accuracy of guided endodontics in removing intraradicular fiberglass posts from posterior mandibular teeth and found that the operator's experience had a significant influence on the accuracy of the procedure. Experienced operators showed greater accuracy and minimal deviation compared to inexperienced operators.
APPLIED SCIENCES-BASEL
(2023)
Article
Dentistry, Oral Surgery & Medicine
Marco Farronato, Andres Torres, Mariano S. Pedano, Reinhilde Jacobs
Summary: The aim of this study is to evaluate the accuracy of a novel augmented reality (AR) method for guided access cavity preparation in 3D-printed jaws. Two operators with different levels of experience performed virtually guided access cavities using an AR system on 3D-printed jaw models. The results show promising results for the use of AR as a digital guide in endodontics, but further research and development may be needed.
JOURNAL OF DENTISTRY
(2023)
Review
Polymer Science
Tristan Hampe, Andreas Wiessner, Holm Frauendorf, Mohammad Alhussein, Petr Karlovsky, Ralf Buergers, Sebastian Krohn
Summary: Dental resins have been widely applied in dentistry, but they may release toxic substances in the oral environment, causing public concern. However, the heterogeneous study design in current research hinders effective biocompatibility testing. Therefore, a reliable experimental and analytical setup is necessary to assess patient exposure and health risks.
Review
Polymer Science
Uros Josic, Claudia Mazzitelli, Tatjana Maravic, Ales Fidler, Lorenzo Breschi, Annalisa Mazzoni
Summary: Incomplete and inadequate removal of endodontic biofilm during root canal treatment often leads to clinical failure. This review provides an overview of endodontic biofilm cultivation methods and discusses the effectiveness of sonic-, ultrasonic-, and Er:Yag laser-assisted biofilm removal techniques. Laser assisted agitation, as well as ultrasonic and sonic activation of the irrigants, are more efficient in biofilm removal compared to conventional irrigation. The choice of irrigant also plays a role in reducing bacterial contamination inside the root canal. Standardization of methodology and introduction of root canals with complex morphology in research are needed for better comparison and recommendations for the most efficient method in biofilm removal.
Review
Engineering, Biomedical
Cristiane Miranda Franca, Gabriela de Souza Balbinot, Diana Cunha, Vicente de Paulo Aragao Saboia, Jack Ferracane, Luiz E. Bertassoni
Summary: Dental caries is a multifactorial and dynamic disease that affects over 90% of adults worldwide. The current treatment for decayed tissue involves using materials to replace lost enamel or dentin. The development and understanding of restorative dental materials rely on in-vitro and in-vivo tests. This review discusses the unique characteristics of the tooth and dentin-pulp interface for material testing, commonly used in-vitro tests for evaluating dental material biocompatibility, and presents future directions for biological research on dental materials using tissue engineering and organs on-a-chip approaches.
ACTA BIOMATERIALIA
(2022)
Article
Dentistry, Oral Surgery & Medicine
Matilde Ruiz-Linares, Javier F. Monroy-Rojas, Carmen Solana, Pilar Baca, Beatriz Aguado, Ana Soriano-Lerma, Maria Teresa Arias-Moliz, Carmen Maria Ferrer-Luque
Summary: This study investigated the antimicrobial efficacy of diclofenac hydrogels in root canal disinfection. The results demonstrated that 5% diclofenac hydrogel showed the highest effectiveness, significantly superior to the other groups.
INTERNATIONAL ENDODONTIC JOURNAL
(2023)
Review
Dentistry, Oral Surgery & Medicine
Jose Luis Sanz, Julia Guerrero-Girones, Maria P. Pecci-Lloret, Miguel R. Pecci-Lloret, Maria Melo
Summary: This study aimed to qualitatively synthesize available in vitro studies assessing the biological interaction of PDLSCs and calcium silicate-based biomaterials. The findings suggest that calcium silicate-based endodontic materials exhibit adequate cytocompatibility and promote the osteo/cementogenic differentiation and mineralization potential of PDLSCs. Further research is needed to explore the impact of compositional differences, additives, sample preparation, and environmental conditions on the biological properties of these materials.
INTERNATIONAL ENDODONTIC JOURNAL
(2021)
Review
Medicine, Research & Experimental
Wencheng Song, Shue Li, Qingming Tang, Lili Chen, Zhenglin Yuan
Summary: Calcium silicate-based bioceramics have been widely used in endodontics due to their excellent properties, affecting cellular behavior and wound healing. Studying their in vitro biocompatibility and bioactivity helps to understand their appropriate application in endodontics.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Article
Health Care Sciences & Services
Hadi Mohammed Alamri, Abdullah Altamimi, Mubashir Baig Mirza, Mazen A. A. Aldosimani, Hani Ghabbani, Fahd Aljarbou
Summary: This study evaluated the ability to detect vertical root fractures (VRFs) using a novel radio-opaque dye and different imaging techniques. The results showed that both periapical radiographs (PARs) and cone-beam computed tomography (CBCT) were equally effective in detecting VRFs. The use of angled radiographs and axial view CBCT improved the detection of VRFs and dye penetration. Further testing is needed before the clinical use of the dye.
Review
Infectious Diseases
Ana Cristina Padilha Janini, Gabriela Fernanda Bombarda, Lauter Eston Pelepenko, Marina Angelica Marciano
Summary: Endodontic biomaterials play a crucial role in dental treatment, but proper clinical procedures are necessary before their application. Future studies will be needed to evaluate the performance of these materials under poly-microbial biofilms associated with endodontic diseases.
Review
Chemistry, Analytical
Jian Ju, Lin Li, Sagar Regmi, Xinyu Zhang, Shixing Tang
Summary: Significant advancements have been made in using microneedle-based diabetes devices for minimally invasive wearable biosensors and continuous glucose monitoring. Recent research has focused on developing in vivo glucose sensors coupled with optical or electrochemical instrumentation. This article discusses the characteristics and applications of these sensors, as well as the challenges and prospects for their future use.
Review
Chemistry, Multidisciplinary
Bernette Maria Oosterlaken, Maria Paula Vena, Gijsbertus de With
Summary: Collagen mineralization is a biological process found in various skeletal elements in the animal kingdom, with a focus on understanding bone formation processes and synthesizing tissue engineering scaffolds. This paper reviews in vitro efforts towards collagen mineralization, primarily centered on hydroxyapatite and silica, with a proposed general mechanism for intrafibrillar mineralization of collagen. Future outlooks for improving collagen-based tissue engineering scaffolds are also discussed.
ADVANCED MATERIALS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Shymaa Shaaban, Gamal M. Hamad, Salma Genena, Marwa A. Meheissen, Sybel Moussa
Summary: This study evaluated the antibacterial activity of Lactobacilli probiotics against Enterococcus faecalis (E. faecalis) and found that Lactobacilli probiotics showed inhibition effect and were more effective compared to calcium hydroxide paste. Lactobacilli probiotics could be a promising intracanal medicament for root canal treatment.
Review
Dentistry, Oral Surgery & Medicine
Julia Guerrero-Girones, Leopoldo Forner, Jose Luis Sanz, Francisco Javier Rodriguez-Lozano, James Ghilotti, Carmen Llena, Adrian Lozano, Maria Melo
Summary: The research on silicate-based endodontic materials has shown exponential growth between 1995 and 2020. The major co-authorship networks are led by Gandolfi MG, Prati C, Rodriguez-Lozano FJ, and Forner L. The most commonly used terms include mineral trioxide aggregate (MTA), biocompatibility, biodentine, and in vitro. The predominance of laboratory studies in this field highlights the need for more clinical studies to validate the findings.
CLINICAL ORAL INVESTIGATIONS
(2022)
Article
Chemistry, Physical
Carmen Llena, Manuel Iglesias-Diaz, Paula Ciscar-Munoz, Ana Belen Bataller-Martinez, Maria Melo, Jose Luis Sanz
Summary: This study evaluated the color change of hydraulic cements after 3 years in regenerative endodontic treatment and the color change after external bleaching at different time points. The results showed a reduction in lightness after 3 years, which gradually recovered after 1 month of bleaching.
Review
Toxicology
Janaina Spoladore, Izabela Gimenes, Rober Bachinski, Jesse P. Negherbon, Thomas Hartung, Jose Mauro Granjeiro, Gutemberg Gomes Alves
Summary: This study aimed to investigate the evidence in the scientific literature for the validity assessments of BET in different medical products. The findings showed that various factors, such as the chemical nature of samples, can influence the outcome of BET, highlighting the need for case-by-case validation for different methods.
TOXICOLOGY IN VITRO
(2021)
Review
Chemistry, Physical
Pantaleo Scelza, Fabiano Goncalves, Isleine Caldas, Fernanda Nunes, Emanuelle Stellet Lourenco, Sandro Tavares, Marcela Magno, Andrea Pintor, Pietro Montemezzi, Emanuele Di Edoardo, Carlos Fernando de Almeida Barros Mourao, Gutemberg Alves, Miriam Zaccaro Scelza
Summary: The study found that Regenerative Endodontic Procedures (REP) showed a high success rate in treating mature teeth with necrotic pulp, reducing periapical lesions and restoring sensitivity. The success levels were similar to those reported for immature teeth, with very low certainty of evidence.
Article
Chemistry, Physical
Sandro Tavares, Andrea Pintor, Carlos Fernando de Almeida Barros Mourao, Marcela Magno, Pietro Montemezzi, Roberto Sacco, Gutemberg Alves, Miriam Zaccaro Scelza
Summary: This review compared the release of growth factors using different root canal irrigant solutions and found that 10% EDTA showed higher levels of TGF-1 release compared to 10% citric acid. However, the evidence certainty was identified as low to very low, demanding further studies before EDTA can be recommended as a better irrigant for regenerative endodontics.
Article
Chemistry, Physical
Ezio Gheno, Gutemberg Gomes Alves, Roberto Ghiretti, Rafael Coutinho Mello-Machado, Antonio Signore, Emanuelle Stellet Lourenco, Paulo Emilio Correa Leite, Carlos Fernando de Almeida Barros Mourao, Dong-Seok Sohn, Monica Diuana Calasans-Maia
Summary: This pilot study investigated the suitability of the Sticky Bone Preparation Device (SBPD(R)) for preparing autologous platelet concentrates and bone substitute materials. The results showed that the SBPD(R) produced sticky bone samples with similar cell content and growth factor release ability compared to hand-mixed samples, but with shorter and simpler preparation times.
Article
Dentistry, Oral Surgery & Medicine
Isleine Portal Caldas, Eduardo Moreira da Silva, Emanuelle Stellet Lourenco, Jean Carlos Martins do Nascimento, Paulo Emilio Correa Leite, Moira Pedroso Leao, Gutemberg Alves, Miriam Zaccaro Scelza
Summary: This study compared the cytocompatibility of two etch-and-rinse and two self-etch dental adhesives and found that the self-etch adhesives exhibited better biocompatibility.
JOURNAL OF DENTISTRY
(2022)
Review
Chemistry, Multidisciplinary
Izabela Gimenes, Andrea V. Braga Pintor, Mariana da Silva Sardinha, Guido A. Maranon-Vasquez, Marcelo Salabert Gonzalez, Octavio Augusto Franca Presgrave, Lucianne Cople Maia, Gutemberg Gomes Alves
Summary: This systematic review examined the effects of different cold corneal storage media (CCSM) compared with Optisol-GS on the quality of stored corneas. The findings suggest that most CCSM perform similarly to Optisol-GS in preserving corneas for transplantation at seven days, but some formulations may improve long-term preservation.
APPLIED SCIENCES-BASEL
(2022)
Article
Dentistry, Oral Surgery & Medicine
Miriam Zaccaro Scelza, Natalia L. P. P. Iorio, Pantaleo Scelza, Helvecio C. C. Povoa, Caroline S. R. Adeodato, Ana Carolina N. Souza, Ana Carolina Batista, Sandro Tavares, Gutemberg Alves
Summary: This study evaluated the antibacterial ability and cytocompatibility of a new irrigant solution consisting of 10% citric acid and 1% chlorhexidine for endodontic treatment. The results showed that the solution was cytocompatible with human periodontal ligament fibroblasts and had comparable antibacterial activity to other commonly used irrigants. The combination of 10% citric acid and 1% chlorhexidine showed potential as a solution for microbiological control during endodontic treatment.
JOURNAL OF DENTISTRY
(2022)
Article
Reproductive Biology
Leticia Pereira Alcaraz, Lucia Prellwitz, Gutemberg Alves, Joanna Maria Gonsalves Souza-Fabjan, Angelo Jose Burla Dias
Summary: Modulation of the PI3K/AKT/PTEN pathway has the potential to improve overall efficiency of in vitro embryo production, but its effects on meiosis resumption are still uncertain.
Article
Health Care Sciences & Services
Renata Norbert Costa Nundes, Humberto Pinheiro de Araujo, Gutemberg Gomes Alves, Isabella Fernandes Delgado, Octavio Augusto Franca Presgrave, Wlamir Correa de Moura, Marcelo Salabert Gonzalez
Summary: This article summarizes alternative methods developed for Bothrops antivenoms and evaluates their potential applications in Bothrops venom and antivenom production and quality control laboratories. The study found that using cell lines as an alternative method to murine assays has been widely preferred and considered as an important tool for quality control of Bothrops venom and antivenom.
VIGILANCIA SANITARIA EM DEBATE-SOCIEDADE CIENCIA & TECNOLOGIA
(2022)
Article
Chemistry, Multidisciplinary
Ana Carolina Batista Brochado, Daniela Costa Silva, Joice Correa da Silva, Adam Lowenstein, Vinicius Schott Gameiro, Elena Mavropoulos, Carlos F. Mourao, Gutemberg Gomes Alves
Summary: This study described the characterization of a 3D spheroid model of MC3T3-E1 murine preosteoblasts for testing bone-substitute materials and investigated the suitability of several cell viability tests. The spheroids showed structural stability, compact surface, and high potential for mineralization but had a reduction in size over time. Colorimetric tests did not correlate with cell density in the 3D model, while the LDH test achieved appropriate optical density and high correlation with cell density, demonstrating the importance of careful selection of test methods for material testing with 3D bone cell models.
APPLIED SCIENCES-BASEL
(2023)
Review
Biotechnology & Applied Microbiology
Carlos Fernando Mourao, Adam Lowenstein, Rafael Coutinho Mello-Machado, Shahram Ghanaati, Nelson Pinto, Tomoyuki Kawase, Gutemberg Gomes Alves, Michel Reis Messora
Summary: Experimental research plays a vital role in advancing medical knowledge and improving patient outcomes. Platelet-rich fibrin (PRF) has gained attention for its potential in tissue regeneration and wound healing. Animal models, such as rabbits and rats, have been used to study PRF and its applications. Standardizing animal models and techniques is crucial for bridging the gap between lab research and clinical applications.
BIOENGINEERING-BASEL
(2023)
Article
Engineering, Biomedical
Kayvon Javid, Carlos Fernando Mourao, Rafael Coutinho Mello-Machado, Suelen Cristina Sartoretto, Madelaine Torres, Emanuelle Stellet Lourenco, Paulo Emilio Correa Leite, Jose Mauro Granjeiro, Gutemberg Gomes Alves, Monica Diuana Calasans-Maia
Summary: This study evaluated the clinical and biochemical effects of heated serum albumin with liquid PRF (Alb-PRF) and L-PRF in socket healing after mandibular third molar extraction. The results showed that Alb-PRF significantly reduced swelling on day seven and had lower levels of pro-inflammatory cytokines compared to L-PRF. Alb-PRF promoted socket healing by reducing edema and the release of inflammatory cytokines.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Review
Engineering, Biomedical
Renata de Lima Barbosa, Emanuelle Stellet Lourenco, Julya Vittoria de Azevedo dos Santos, Neilane Rodrigues Santiago Rocha, Carlos Fernando Mourao, Gutemberg Gomes Alves
Summary: Platelet-rich fibrin (PRF) is an autologous approach for tissue regeneration that promotes cell interaction and growth factor release. In vitro evidence suggests that PRF has a positive effect on bone cell behavior, including proliferation, differentiation, and mineralization. This reinforces PRF's therapeutic potential in wound healing and bone regeneration.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Review
Health Care Sciences & Services
Margareth Timoteo, Emanuelle Lourenco, Ana Carolina Brochado, Luciana Domenico, Joice da Silva, Bruna Oliveira, Renata Barbosa, Pietro Montemezzi, Carlos Fernando de Almeida Barros Mourao, Beni Olej, Gutemberg Alves
Summary: This review identified digital tools for managing academic health sciences and experimental medicine laboratories and their potential contribution to compliance with the principles of good laboratory practices (GLP), improving workflow and addressing limitations during implementation.
Article
Dentistry, Oral Surgery & Medicine
Larissa Maria Cavalcante, Amanda Barreto Ramos, Daniela Costa Silva, Gutemberg Gomes Alves, Karinne Bueno Antunes, Carmem Silvia Pfeifer, Luis Felipe Jochims Schneider
Summary: The study found that thiourethane-functionalized fillers did not significantly affect the antimicrobial properties and cytotoxicity of experimental composites, and these materials showed equivalent properties to those conventionally used in dentistry.
BRAZILIAN ORAL RESEARCH
(2021)