Review
Cell Biology
Ivana Vrhovac Madunic, Valentina Karin-Kujundzic, Josip Madunic, Ida Marija Sola, Ljiljana Serman
Summary: Glucose metabolism in the endometrium plays a crucial role in pregnancy outcomes, with ten glucose transporters expressed in rodents and humans, including GLUT1, GLUT3, GLUT4, and GLUT8. The recent discovery of SGLT1 in the mouse and human endometrium emphasizes its importance in regulating glycogen accumulation for embryo implantation. Investigating the function of glucose transporters in different endometrial cells is essential, as they are associated with infertility, polycystic ovary syndrome, and gestational diabetes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pathology
Kyriakos Chatzopoulos, Marie-Christine Aubry, Sounak Gupta
Summary: von Hippel-Lindau (VHL) disease is characterized by cysts and tumors in multiple organ systems. The loss of function of the VHL tumor suppressor gene leads to the upregulation of the HIF1a pathway, as indicated by the overexpression of CA9 and GLUT1 in VHL disease-related lesions. PAX8 is only expressed in renal and epididymal lesions.
Article
Chemistry, Multidisciplinary
Jingjing Wang, Zhaoli Sun, Shuren Wang, Chenyang Zhao, Junjie Xu, Shen Gao, Meng Yang, Fugeng Sheng, Song Gao, Yanglong Hou
Summary: The study introduces a tumor therapy strategy that disrupts intratumoral metabolic symbiosis by inducing acidosis for tumor elimination, using carbonic anhydrase inhibitor-modified ferrous sulfide nanoparticles. The nanoparticles also have dual-mode imaging capabilities.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Analytical
Ririko Nakaoka, Kazuhiro Kato, Kumiko Yamamoto, Hironobu Yasui, Shingo Matsumoto, Igor A. Kirilyuk, Valery V. Khramtsov, Osamu Inanami, Hiroshi Hirata
Summary: Extracellular acidification is a hallmark of tumor malignancy, and non-invasive mapping of extracellular pH (pHe) is essential for understanding the tumor microenvironment and monitoring tumor response to metabolism-targeting drugs. A modified electron paramagnetic resonance (EPR) system achieves higher sensitivity in mapping pHe and can be used to monitor the response of other metabolism-targeting drugs.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jun Yan, Kaizhen Wang, Lijuan Gui, Xian Liu, Yingying Ji, Jingjing Lin, Man Luo, Hong Xu, Jingxuan Lv, Fang Tan, Liangting Lin, Zhenwei Yuan
Summary: Nitroreductase (NTR) is an enzyme that is upregulated under tumor-depleted oxygen conditions. In this study, we developed a novel near-infrared (NIR) fluorescent probe IR-ABS that jointly targets NTR and carbonic anhydrase IX (CAIX). The probe showed excellent sensitivity and selectivity and demonstrated good biosafety in both cells and mice. Furthermore, the probe was successfully applied for NTR detection in orthotopic lung tumor models.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Shuai Ma, Fang Wang, Jiawei Dong, Nan Wang, Shengzhong Tao, Jianyang Du, Shaoshan Hu
Summary: This study demonstrates that the combination of photodynamics therapy (PDT) and Aciflavine (ACF) can decrease the expression of HIF1α and regulate the downstream signaling pathways, thereby enhancing the efficacy of PDT in treating recalcitrant glioblastoma (GBM).
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Katarina Grossmannova, Monika Barathova, Petra Belvoncikova, Viliam Lauko, Lucia Csaderova, Jan Tomka, Tomas Dulka, Jaromir Pastorek, Juraj Madaric
Summary: Abdominal aortic aneurysms (AAA) are a significant cause of premature deaths worldwide. In this study, the presence of hypoxia-related protein CA IX in AAA tissues was confirmed, suggesting its potential role in aneurysmal wall remodeling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Lu-Yi Yu, Pei-Wei Shueng, Hsin-Cheng Chiu, Yu-Wei Yen, Tzu-Yu Kuo, Chieh-Ru Li, Ming-Wei Liu, Chia-Hsin Ho, Tzu-Hao Ho, Bo-Wei Wang, Cheng-En Li, Ming-Hung Chen, Yao-An Shen, Chun-Liang Lo
Summary: Tumour hypoxia plays a crucial role in various aspects of cancer progression, including angiogenesis, invasion, immunosuppression, and stemness maintenance. By targeting the glucose transporter 1 (GLUT1)-mediated transcytosis, a potential therapeutic strategy for tumor hypoxia was developed. Glucosamine-labeled liposomal ceramide was efficiently transported by GLUT1 and accumulated in the hypoxic area, while exogenous ceramide showed significant effects on tumor hypoxia and disrupted key signaling pathways.
Article
Obstetrics & Gynecology
Yao-Lung Chang, An-Shine Chao, Shuenn-Dyh Chang, Po-Jen Cheng
Summary: This study aimed to investigate the expression of placental GLUT1 and GLUT3 genes in MC twin gestations with selective FGR. It was found that in twin pregnancies with abnormal UA Doppler in the selective FGR fetus, GLUT3 and HIF-1 alpha gene expression were significantly elevated. This may be attributed to hypoperfusion mediated by up-regulation of HIF-1 alpha gene expression.
BMC PREGNANCY AND CHILDBIRTH
(2021)
Article
Medicine, Research & Experimental
Daan F. Boreel, Paul N. Span, Annemarie Kip, Milou Boswinkel, Johannes P. W. Peters, Gosse J. Adema, Johan Bussink, Sandra Heskamp
Summary: Limited oxygen diffusion and increased oxygen consumption in solid malignancies lead to chronic hypoxia, which induces radioresistance and an immunosuppressive microenvironment. This study aimed to develop a radiolabeled antibody to visualize chronic hypoxia and study immune cell populations in these hypoxic areas. The results showed that the radiotracer binds to CAIX-expressing cells in vitro and accumulates in CAIX+ areas in vivo. The technique can quantitatively distinguish between tumor models with varying CAIX+ fractions and identified less infiltration of immune cells in the CAIX+ areas.
MOLECULAR PHARMACEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Barbora Puzderova, Petra Belvoncikova, Katarina Grossmannova, Lucia Csaderova, Martina Labudova, Silvia Fecikova, Jaromir Pastorek, Monika Barathova
Summary: Resistance to chemotherapy is a major obstacle in cancer treatment. The non-selective beta-blocker propranolol has the potential to overcome drug resistance by disrupting tumor adaptation and activating apoptosis. It can serve as a chemosensitizer in combined therapy to disrupt tumor microenvironment homeostasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Md. Abu Sufian, Sabina Zamanova, Ahmed M. Shabana, Brianna Kemp, Utpal K. Mondal, Claudiu T. Supuran, Marc A. Ilies
Summary: Carbonic anhydrase IX (CA IX) is an important enzyme involved in tumor survival, metastasis, and resistance to therapy in hypoxic tumor cells. The expression dynamics of CA IX in different oxygen conditions were investigated, along with its correlation with extracellular pH and cell viability. The study found that CA IX expression is retained in hypoxic cancer cells upon reoxygenation to preserve their proliferation ability. The sensitivity of cancer cells to CA IX inhibitors is higher in hypoxia and intermittent hypoxia compared to normoxia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Miriam Zatovicova, Ivana Kajanova, Monika Barathova, Martina Takacova, Martina Labudova, Lucia Csaderova, Lenka Jelenska, Eliska Svastova, Silvia Pastorekova, Adrian L. Harris, Jaromir Pastorek
Summary: Hypoxia in the tumor microenvironment is a major factor in cancer progression, and low levels of oxygen may indicate treatment failure. Carbonic anhydrase IX (CA IX) is a precise target in hypoxic tumors, and its presence contributes to the failure of standard therapy. Humanized antibodies against CA IX show promise as suitable therapies for hypoxic tumors, with the ability to modulate the tumor microenvironment, induce an immune response, and block metastasis formation.
CANCER & METABOLISM
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Julia C. Carmona-Bozo, Roido Manavaki, Jodi L. Miller, Cara Brodie, Corradina Caraco, Ramona Woitek, Gabrielle C. Baxter, Martin J. Graves, Tim D. Fryer, Elena Provenzano, Fiona J. Gilbert
Summary: This study explores the relationship between indices of hypoxia and vascular function in ER + breast cancer using [F-18]-FMISO PET/MRI and immunohistochemical markers. The results indicate that hypoxia is associated with increased CAIX expression, reduced microvessel density, and smaller vessel diameters in ER + breast cancer, further confirming the link between inadequate vascularity and hypoxia in this type of cancer.
EUROPEAN RADIOLOGY
(2023)
Article
Biology
Ying-Yi Lu, Chieh-Hsin Wu, Chien-Hui Hong, Kee-Lung Chang, Chih-Hung Lee
Summary: The study revealed that glycolysis was enhanced in keloid fibroblasts, while oxygen consumption rate remained unchanged. GLUT-1 expression was selectively increased in proliferating keloid fibroblasts, potentially contributing to cell proliferation. Inhibiting GLUT-1 could reduce ROS levels and cell proliferation in keloid fibroblasts.
Article
Multidisciplinary Sciences
Magdalena Kostrzewska-Poczekaj, Kinga Bednarek, Malgorzata Jarmuz-Szymczak, Magdalena Bodnar, Violeta Filas, Andrzej Marszalek, Anna Bartochowska, Reidar Grenman, Katarzyna Kiwerska, Krzysztof Szyfter, Maciej Giefing
SCIENTIFIC REPORTS
(2020)
Review
Medicine, Research & Experimental
Martyna Parol, Arkadiusz Gzil, Magdalena Bodnar, Dariusz Grzanka
Summary: The process of epithelial-mesenchymal transition affects tumor cell spread and migration, with miRNA molecules playing a crucial role in regulating signaling pathways in the tumor microenvironment. MiRNAs have potential diagnostic and therapeutic implications for PCa, with studies showing a correlation between miRNA expression and clinical features as well as metastasis in PCa patients.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Cell & Tissue Engineering
Tomasz Kloskowski, Joanna Jarzabkowska, Arkadiusz Jundzill, Daria Balcerczyk, Monika Buhl, Kamil Szeliski, Magdalena Bodnar, Andrzej Marszalek, Gerard Drewa, Tomasz Drewa, Marta Pokrywczynska
STEM CELLS INTERNATIONAL
(2020)
Article
Multidisciplinary Sciences
Arkadiusz Jundzill, Henryk Witmanowski, Ewa Zary-Sikorska, Jan Adamowicz, Magdalena Bodnar, Andrzej Marszalek, Tomasz Kloskowski, Kaja Mecinska-Jundzill, Maciej Gagat, Natalia Siedlecka, Tomasz Drewa, Marta Pokrywczynska
Summary: This study developed a new procedure for urinary bladder transplantation on a rat model, achieving a high success rate and observing no complications two weeks post-surgery. The research aimed to acquire necessary knowledge about urinary bladder transplantation and proposed a technique that offers a new approach to existing orthotopic models.
SCIENTIFIC REPORTS
(2021)
Article
Pathology
Ewelina Bartkowiak, Krzysztof Piwowarczyk, Magdalena Bodnar, Pawel Kosikowski, Jadzia Chou, Aldona Wozniak, Malgorzata Wierzbicka
Summary: The study revealed significant differences in p16(Ink4a) expression between normal tissue, 'fast' and 'slow' pleomorphic adenomas. Overexpression of p16(Ink4a) may be related to proliferation of pleomorphic adenoma and subsequent malignant transformation to CaexPA. Borderline p16(Ink4a) staining correlates with worse prognosis of CaexPA.
JOURNAL OF CLINICAL PATHOLOGY
(2022)
Article
Engineering, Biomedical
Marta Pokrywczynska, Arkadiusz Jundzill, Jakub Tworkiewicz, Monika Buhl, Daria Balcerczyk, Jan Adamowicz, Tomasz Kloskowski, Marta Rasmus, Kaja Mecinska-Jundzill, Damian Kasinski, Malgorzata Frontczak-Baniewicz, Marcin Holysz, Joanna Skopinska-Wisniewska, Magdalena Bodnar, Andrzej Marszalek, Paulina Antosik, Dariusz Grzanka, Tomasz Drewa
Summary: The study evaluated the utility of bladder acellular matrix (BAM) for reconstructing large urinary bladder wall defects in a long-term porcine model. Six out of ten animals survived the 6-month follow-up, with four dying due to scaffold failure or other complications. Reconstructed bladders showed normal function with lower expression of urothelial, smooth muscle, blood vessel, and nerve markers compared to the native bladder.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2022)
Article
Multidisciplinary Sciences
Martyna Parol-Kulczyk, Arkadiusz Gzil, Joanna Maciejewska, Magdalena Bodnar, Dariusz Grzanka
Summary: Chronic inflammation can induce carcinogenesis through the secretion of factors by inflammatory response cells and induction of epithelial-mesenchymal transition. The study on FFPE tissues from prostate cancer patients showed correlations between protein expressions of MIF, beta-catenin, and the occurrence of lymph node and distant metastasis.
Article
Biochemistry & Molecular Biology
Joanna Janiszewska, Magdalena Bodnar, Julia Paczkowska, Adam Ustaszewski, Maciej J. Smialek, Lukasz Szylberg, Andrzej Marszalek, Katarzyna Kiwerska, Reidar Grenman, Krzysztof Szyfter, Malgorzata Wierzbicka, Maciej Giefing, Malgorzata Jarmuz-Szymczak
Summary: The study demonstrates that miR-1290 suppresses MAF expression in LSCC, revealing the underlying interaction between the two. Immunohistochemistry staining showed loss of nuclear MAF expression in 58% of LSCC samples. Through data analysis, it was determined that loss-of-function mutations, promoter region DNA methylation or CNV are not responsible for MAF loss in LSCC.
Editorial Material
Medicine, General & Internal
Pawel Sadlecki, Marek Grabiec, Malgorzata Walentowicz-Sadlecka
Summary: Broad ligament pregnancy is a rare life-threatening form of ectopic pregnancy where implantation occurs within the peritoneal cavity. The laparoscopic approach offers advantages over laparotomy such as reduced blood loss, shorter operating time, lower analgesic requirements, shorter hospital stay, and quicker recovery.
CLINICAL CASE REPORTS
(2021)
Article
Oncology
Jedrzej Borowczak, Krzysztof Szczerbowski, Mateusz Maniewski, Marek Zdrenka, Piotr Slupski, Paulina Antosik, Sylwia Kolodziejska, Marta Sekielska-Domanowska, Mariusz Dubiel, Magdalena Bodnar, Lukasz Szylberg
Summary: This article investigates the prognostic role of cyclin-dependent kinase 9 expression in urothelial carcinoma. The results show that high CDK9 expression is associated with a better prognosis, tumor grade, stage, and invasiveness, contradicting previous findings. CDK9 may play a crucial role in the early stages of urothelial carcinoma.
Article
Virology
Pawel Solarz, Magdalena Bodnar, Joanna Czech, Hanna Mackiewicz-Nartowicz, Anna Sinkiewicz, Lukasz Szylberg, Jedrzej Borowczak, Paula Rutkiewicz, Aleksander Zwierz, Pawel Burduk
Summary: Recurrent respiratory papillomatosis is a disease closely related to human papillomavirus infection. The main treatment for lesions in the larynx or lower pharynx is microsurgical excision using a CO2 laser, but combined antiviral therapy can reduce the number of surgeries and prevent regrowth of the lesions.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Review
Medicine, General & Internal
Pawel Sadlecki, Malgorzata Walentowicz-Sadlecka
Summary: Congenital malformations refer to defects in the formation of organs or body parts that can be identified during intrauterine life or at birth. Advances in prenatal detection have made it possible to identify many of these disorders early through routine fetal ultrasound. This systematic review aims to organize current knowledge on the mode of delivery in pregnancies with fetal anomalies. After analyzing 546 studies, 18 articles on delivery mode and neonatal outcomes were chosen. Spontaneous vaginal delivery is generally recommended for most pregnancies with fetal anomalies, while cesarean delivery may be indicated for certain anomalies that carry risks such as dystocia, bleeding, or rupture of protective sac. Early fetal anatomy ultrasound is crucial in providing parents with all available options, including pregnancy termination, if an anomaly is detected.
Article
Oncology
Jedrzej Borowczak, Krzysztof Szczerbowski, Mateusz Maniewski, Marek Zdrenka, Piotr Slupski, Hanna Andrusewicz, Joanna Lysik-Miskurka, Paula Rutkiewicz, Magdalena Bodnar, Lukasz Szylberg
Summary: This study found that high expression of p53 is associated with unfavorable clinical features in bladder cancer and is correlated with the expression of CDK9. CDK9 may influence p53 expression through interactions with p53 inhibitors. Confirming the crosstalk between p53 and CDK9 in bladder cancer may be an important step in explaining the mechanism of tumor progression in its early stages.
CLINICAL & TRANSLATIONAL ONCOLOGY
(2023)
Article
Medicine, General & Internal
Pawel Walentowicz, Pawel Sadlecki, Malgorzata Walentowicz-Sadlecka, Anna Bajek, Marek Grabiec, Tomasz Drewa
Summary: This study analyzed the effects of maternal, fetal, and environmental factors on the success rates and characteristics of amniotic fluid stem cell cultures. The results showed that the volume of the amniotic fluid sample, the presence of two cell populations, and the season of the year were significantly associated with culture success and the number of cell passages. Individual characteristics of the donors, such as age and gravidity, did not have a significant effect on the isolated stem cells and their growth rates.
Article
Medicine, Research & Experimental
Przemyslaw Galazka, Lukasz Szylberg, Magdalena Bodnar, Jan Styczynski, Andrzej Marszalek