Article
Cell Biology
Benyu Su, David Lim, Chenyang Qi, Zhongwei Zhang, Junxiao Wang, Fengmei Zhang, Chao Dong, Zhihui Feng
Summary: Cell cycle checkpoint kinases play a crucial role in protecting against replicative stress. Valproic acid (VPA), a histone deacetylase inhibitor, was found to promote breast cancer cell progression into G2/M phase while protecting normal cells by regulating PPP2R2A and Chk1 phosphorylation. The expression levels of PPP2R2A and pChk1 were also associated with patient survival.
CELL DEATH & DISEASE
(2023)
Review
Immunology
Saulo Brito Silva, Carlos Wagner S. Wanderley, Leandro Machado Colli
Summary: Cancer cells have genomic instability due to accumulated DNA damage. Multiple DNA damage repair pathways exist to address DNA damage during different cell cycle stages. Immunological checkpoint inhibitors have shown great efficacy in tumors with mismatch repair deficiency, but there are differences in efficacy and resistance to treatment in tumors with homologous recombination deficiency.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Mi Ae Kang, Jong-Soo Lee
Summary: CTCF, a highly conserved multifunctional DNA-binding protein with 11 zinc fingers, plays crucial roles in diverse genomic processes such as transcriptional regulation, insulation, genome imprinting, and maintenance of genome organization. Recent findings reveal that CTCF is involved in DNA double-strand break (DSB) repair through homologous recombination (HR), facilitating accurate restoration of broken DNA sequences. Understanding the functional crosstalks between CTCF and other HR factors may shed light on the molecular basis of various human diseases and the diverse functions of CTCF in genome biology.
Article
Multidisciplinary Sciences
Donna R. Whelan, Eli Rothenberg
Summary: Using single-molecule localization super-resolution imaging assays, we observed the spatiotemporal behavior of key mediator and nuclease proteins during DNA resection at single-ended double-strand breaks. Multiple simultaneous resection events were demonstrated, with recruitment of various proteins and completion of resection 2 to 4 hours after break induction. Additionally, we identified potential roles of BRCA1 and BLM in homology search and repair resolution during HR.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Michael T. Kimble, Matthew J. Johnson, Mattie R. Nester, Lorraine S. Symington
Summary: Exo1 and Sgs1 are involved in long-range resection during HR, which is connected to the activation of the DNA damage checkpoint and the mobility of chromosomes.
Article
Multidisciplinary Sciences
Xuanpeng Wang, Lili Wang, Yongchi Huang, Zhiping Deng, Cunliang Li, Jian Zhang, Mingxi Zheng, Shunping Yan
Summary: This study reveals the important role of a plant-specific ubiquitin E3 ligase, DDRM1, in homologous recombination repair (HR), which is essential for maintaining genome stability. DDRM1 interacts with and ubiquitinates the transcription factor SOG1, promoting its stability. This research uncovers the regulatory mechanism of HR in plants and highlights the significance of ubiquitination in HR.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Oncology
Jiaojiao Deng, Sophia B. Chernikova, Yuelong Wang, Mirna L. Rodriguez, Stephanie J. Andersen, Maxine C. Umeh-Garcia, Bryanna O. Godfrey, Saman S. Ahmadian, Wolf-Nicolas Fischer, Kerry J. Koller, Bernd Jandeleit, Gordon M. Ringold, Melanie Hayden Gephart
Summary: Metastasis to the central nervous system from breast cancer, especially triple-negative breast cancer, presents a significant clinical challenge due to limited treatment options and rapidly fatal outcomes. Novel therapeutic options that can effectively target brain metastasis and leptomeningeal spread are urgently needed in order to improve patient outcomes.
MOLECULAR CANCER THERAPEUTICS
(2021)
Review
Biochemistry & Molecular Biology
Mengting Chen, Renske Linstra, Marcel A. T. M. van Vugt
Summary: Genomic and chromosomal instability are characteristics of cancer that affect the genetic composition of cancer cells, influencing their behavior and response to treatment. This instability can lead to DNA leakage into the cytoplasm, triggering inflammatory signaling pathways that have pleiotropic effects, including enhanced anti-tumor immunity and potential sensitization of cancer cells to immune checkpoint inhibitors.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2022)
Article
Oncology
Arathi Rajan, Geetu R. Varghese, Induprabha Yadev, Jaimie Anandan, Neetha R. Latha, Dipyaman Patra, Neethu Krishnan, Krithiga Kuppusamy, Arathy Warrier, Satej Bhushan, Revathy Nadhan, Ram Mohan Ram Kumar, Priya Srinivas
Summary: BRCA1 mutation carriers have a higher risk of developing breast and ovarian cancers, but the underlying mechanisms remain unclear. This study found that in breast cancer cells, E2 and ER-α signaling can enhance BRCA1-mediated DNA repair, while deficiency in ER-α delays DNA damage repair and promotes tumor progression.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)
Article
Oncology
Miguel A. Villalona-Calero, John P. Diaz, Wenrui Duan, Zuanel Diaz, Eric D. Schroeder, Santiago Aparo, Troy Gatcliffe, Federico Albrecht, Siddhartha Venkatappa, Victor Guardiola, Sara Garrido, Muni Rubens, Fernando DeZarraga, Hao Vuong
Summary: This study demonstrates the significant antitumor activity of pembrolizumab in malignancies without current FDA approved indications and with functional Fanconi anemia deficiency. The results support further evaluation of FATSI as a discriminatory biomarker for population-selected studies.
BIOMARKER RESEARCH
(2022)
Article
Oncology
Jianfeng He, Caihu Huang, Yanmin Guo, Rong Deng, Lian Li, Ran Chen, Yanli Wang, Jian Huang, Junke Zheng, Xian Zhao, Jianxiu Yu
Summary: SUMOylated PTEN promotes homologous recombination (HR) repair and represses nonhomologous end joining (NHEJ) repair. It dephosphorylates TP53-binding protein 1 (53BP1) to regulate HR repair. Furthermore, SUMOylated PTEN directly and specifically dephosphorylates a specific site on 53BP1, facilitating DNA end resection and ongoing HR repair.
MOLECULAR ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Wanjuan Feng, Dennis A. Simpson, Jang-Eun Cho, Juan Carvajal-Garcia, Chelsea M. Smith, Kathryn M. Headley, Nate Hathaway, Dale A. Ramsden, Gaorav P. Gupta
Summary: Genome integrity and genome engineering require efficient repair of DNA double-strand breaks through non-homologous end joining (NHEJ), homologous recombination (HR), or alternative end-joining pathways. Two complementary methods are described in this study for marker-free quantification of DSB repair pathway utilization at Cas9-targeted chromosomal DSBs in mammalian cells. These methods enable comprehensive assessment of repair pathway utilization and are adaptable to any cellular model system and genomic locus where Cas9-mediated targeting is feasible.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Oncology
Marcin Herok, Bartosz Wawrzynow, Marta J. Maluszek, Maciej B. Olszewski, Alicja Zylicz, Maciej Zylicz
Summary: MDM2 plays a crucial role in cancer development, involved in DNA repair inhibition and chemoresistance acquisition. Inhibiting MDM2 expression can reduce the chemoresistance of tumor cells.
Review
Genetics & Heredity
Anton Gartner, JoAnne Engebrecht
Summary: Accurate DNA replication and propagation are crucial for the faithful transmission of genetic information. Various DNA repair and recombination pathways have evolved to repair DNA lesions generated by normal cellular function or external agents. Checkpoint pathways monitor the genome and coordinate repair processes. The nematode Caenorhabditis elegans is an ideal model organism for studying DNA repair, recombination, and checkpoint signaling. Understanding these processes in C. elegans provides insights into development, growth, and germ line function, as well as informing cancer mutational signatures in humans.
Article
Oncology
Susana Banerjee, James Stewart, Nuria Porta, Christy Toms, Alexandra Leary, Stephanie Lheureux, Saira Khalique, Jeremy Tai, Ayoma Attygalle, Katherine Vroobel, Christopher J. Lord, Rachael Natrajan, Judith Bliss
Summary: The study aims to investigate the clinical activity of the ATR inhibitor, ceralasertib, as a single agent and in combination with the PARP inhibitor, olaparib, in patients with ARID1A 'loss' and 'no loss' clear cell carcinomas and other relapsed gynecological cancers.
INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
(2021)
Article
Pharmacology & Pharmacy
Mingming Gao, Chunbo Zhang, Yongjie Ma, Dexi Liu
Review
Pharmacology & Pharmacy
Chunbo Zhang, Patrick Kwan, Zhong Zuo, Larry Baum
ADVANCED DRUG DELIVERY REVIEWS
(2012)
Article
Clinical Neurology
Chunbo Zhang, Zhong Zuo, Patrick Kwan, Larry Baum
Article
Clinical Neurology
Chunbo Zhang, Hugues Chanteux, Zhong Zuo, Patrick Kwan, Larry Baum
Article
Clinical Neurology
Pui Shan Chan, Chunbo Zhang, Zhong Zuo, Patrick Kwan, Larry Baum
Article
Genetics & Heredity
Larry Baum, Batoul Sadat Haerian, Ho-Keung Ng, Virginia C. N. Wong, Ping Wing Ng, Colin H. T. Lui, Ngai Chuen Sin, Chunbo Zhang, Brian Tomlinson, Gary Wing-Kin Wong, Hui Jan Tan, Azman Ali Raymond, Zahurin Mohamed, Patrick Kwan
Article
Biotechnology & Applied Microbiology
Mingming Gao, Chunbo Zhang, Yongjie Ma, Le Bu, Linna Yan, Dexi Liu
Article
Biochemistry & Molecular Biology
Chunbo Zhang, Guisheng Zhang, Dexi Liu
Article
Pharmacology & Pharmacy
Ziyan Fei, Mengyun Hu, Larry Baum, Patrick Kwan, Tao Hong, Chunbo Zhang
Article
Clinical Neurology
Chunbo Zhang, Patrick Kwan
FRONTIERS IN NEUROLOGY
(2019)
Article
Medicine, Research & Experimental
Bingxin Lu, Jianing Zhong, Jianfei Pan, Xiaopeng Yuan, Mingzhi Ren, Liping Jiang, Yuqing Yang, Guisheng Zhang, Dexi Liu, Chunbo Zhang
JOURNAL OF TRANSLATIONAL MEDICINE
(2019)
Article
Biology
Chunbo Zhang, Tianyu Zhong, Yuanyuan Li, Xianfeng Li, Xiaopeng Yuan, Linlin Liu, Weilin Wu, Jing Wu, Ye Wu, Rui Liang, Xinhua Xie, Chuanchuan Kang, Yuwen Liu, Zhonghong Lai, Jianbo Xiao, Zhixian Tang, Riqun Jin, Yan Wang, Yongwei Xiao, Jin Zhang, Jian Li, Qian Liu, Zhongsheng Sun, Jianing Zhong
Summary: TET1 interacts with SIRT1 to regulate hepatic gluconeogenic gene expression, and the AMPK-TET1-SIRT1 axis plays a role in promoting glucose metabolism.
Article
Plant Sciences
Yuanfeng Lyu, Dan Li, Ziwei Li, Jun Zhang, Xing Ming, Pang Chui Shaw, Chunbo Zhang, Alice Pik Shan Kong, Zhong Zuo
Summary: This study aimed to investigate the effect of combination use of metformin with berberine on hypoglycemic activity and identify the possible intestinal bacteria involved in their microbiotamedicated drug-drug interactions in db/db mice. The results showed that the combination treatment of metformin with berberine could further reduce blood glucose and cause different alterations on gut microbial communities in comparison to their individual treatment.
Article
Medicine, Research & Experimental
Mingzhi Ren, Jianfei Pan, Xueying Yu, Kaile Chang, Xiaopeng Yuan, Chunbo Zhang
Summary: The study shows that CTRP1 improves glucose homeostasis, prevents obesity and fatty liver by increasing energy expenditure and reducing food intake. It also exerts anti-inflammatory effects by regulating gene expression and signaling pathways in adipose tissue.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)