Article
Biochemistry & Molecular Biology
Khushboo Borah, Tom A. Mendum, Nathaniel D. Hawkins, Jane L. Ward, Michael H. Beale, Gerald Larrouy-Maumus, Apoorva Bhatt, Martine Moulin, Michael Haertlein, Gernot Strohmeier, Harald Pichler, V. Trevor Forsyth, Stephan Noack, Celia W. Goulding, Johnjoe McFadden, Dany J. Beste
Summary: Mycobacterium tuberculosis requires co-catabolism of host-derived carbon substrates for infection, showing metabolic plasticity in utilizing cholesterol or glycerol in combination with two-carbon generating substrates. The partitioning of flux between the TCA cycle, glyoxylate shunt, and reversible methyl citrate cycle are critical metabolic nodes for Mtb’s nutritional flexibility. These findings provide novel insights into Mtb's adaptability to divergent carbon substrates.
MOLECULAR SYSTEMS BIOLOGY
(2021)
Review
Immunology
Amanda N. N. Samuels, Erin R. R. Wang, Gregory A. A. Harrison, Joy C. C. Valenta, Christina L. L. Stallings
Summary: This article discusses the impact of bacterial central carbon metabolism on Mtb drug tolerance and proposes that these metabolic pathways could be potential drug targets. By better understanding the physiological state of Mtb in the host, we can hope to treat tuberculosis more effectively.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Jin-Feng Pei, Nan Qi, Yu-Xin Li, Jing Wo, Bang-Ce Ye
Summary: Research has found that nutrient metabolism plays a crucial role in the pathogenesis of Mycobacterium tuberculosis. By studying its fast-growing counterpart, Mycobacterium smegmatis, the phosphate sensing regulator RegX3 was identified to be essential for propionate metabolism and survival in macrophages. This novel transcriptional regulation mechanism sheds light on nutrient sensing and adaptation in mycobacteria.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Terry Kipkorir, Gabriel T. Mashabela, Timothy J. de Wet, Anastasia Koch, Lubbe Wiesner, Valerie Mizrahi, Digby F. Warner
Summary: Cobalamin plays a crucial role in regulating metabolism and gene expression in M. smegmatis, affecting the synthesis of methionine through the control of MetE expression. The bacterium is capable of de novo cobalamin biosynthesis during aerobic growth and limited uptake of exogenous cyanocobalamin and its precursors. The metabolic differences observed between M. smegmatis and M. tuberculosis provide valuable insights into the evolution of mycobacterial metabolism for pathogenicity.
JOURNAL OF BACTERIOLOGY
(2021)
Review
Immunology
Paolo Miotto, Rita Sorrentino, Stefano De Giorgi, Roberta Provvedi, Daniela Maria Cirillo, Riccardo Manganelli
Summary: Bacterial drug resistance poses a serious threat to human health, with tuberculosis being a high-risk infection due to limited drugs available against Mycobacterium tuberculosis. Drug resistance in bacteria is often caused by point mutations in drug targets, but changes in transcriptional regulation post-drug exposure also play a significant role and should be further explored for potential new drug targets and intervention strategies.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Ritu Sharma, Travis E. Hartman, Tiago Beites, Jee-Hyun Kim, Hyungjin Eoh, Curtis A. Engelhart, Linnan Zhu, Daniel J. Wilson, Courtney C. Aldrich, Sabine Ehrt, Kyu Young Rhee, Dirk Schnappinger
Summary: The current treatment for tuberculosis (TB) infections is lengthy and requires multiple antibiotics. The development of shorter, simpler treatment regimens is critical to eradicating TB. NadE, an enzyme essential for the synthesis of the cofactor NAD, is considered a promising drug target. Through gene silencing and metabolomics, it has been shown that NADP deficiency is bacteriostatic, while NAD deficiency is bactericidal due to its role in Mtb's respiratory capacity. These findings suggest prioritizing NAD biosynthesis inhibitors in anti-TB drug development.
Article
Microbiology
Gopinath Krishnamoorthy, Peggy Kaiser, Patricia Constant, Ulrike Abu Abed, Monika Schmid, Christian K. Frese, Volker Brinkmann, Mamadou Daffe, Stefan H. E. Kaufmann
Summary: Mycofactocin, a peptide-derived redox cofactor, is found in certain bacteria like Mycobacterium tuberculosis. Research indicates that mftD gene expression is upregulated in hypoxia-adapted M. tuberculosis, but its functionality may not be essential for L-lactate metabolism. The study highlights the potential significance of mycofactocin in bacterial growth and persistence within the host.
Article
Biochemistry & Molecular Biology
Wenping Wei, Xiaofang Jiang, Li Zhang, Yunjun Yan, Jinyong Yan, Li Xu, Chun-Hui Gao, Min Yang
Summary: A transcription factor called CasR was identified in this study, which can bind to the upstream DNA sequence of the CRISPR-Cas gene cluster and regulate the expression of cas genes. The findings provide new insights into the regulatory effect of CasR on the expression of CRISPR-associated genes in mycobacteria.
Review
Biochemistry & Molecular Biology
Manluan Sun, Sai Ge, Zhaoyang Li
Summary: This paper systematically summarizes the impacts of protein acylation and phosphorylation on the acquisition of drug resistance in Mycobacterium tuberculosis through acylation and phosphorylation protein regulating processes. This provides us with a better understanding of the mechanism of antituberculosis drugs and may contribute to a reduction in the harm that tuberculosis brings to society, as well as aiding in the discovery of new drug targets and therapeutic regimen adjustments in the future.
Review
Plant Sciences
Angeles Aroca, Inmaculada Garcia-Diaz, Margarita Garcia-Calderon, Cecilia Gotor, Antonio J. Marquez, Marco Betti
Summary: Photorespiration plays a fundamental role in plant metabolism and its regulation, including transcriptional and post-translational regulation, has been a topic of interest. The interconnections between photorespiration and nitrogen and sulfur metabolism have also been explored. Recent findings on sulfide signaling and protein persulfidation are discussed.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Immunology
Lerato Hlaka, Mumin Ozturk, Julius E. Chia, Shelby-Sara Jones, Shandre Pillay, Sibongiseni K. L. Poswayo, Thabo Mpotje, Justin K. Nono, Simphiwe R. N. Simelane, Suraj P. Parihar, Sugata Roy, Harukazu Suzuki, Frank Brombacher, Reto Guler
Summary: IL-4i1 deletion in mice improves protection against Mycobacterium tuberculosis infection, reduces bacterial burden, and alters immune cell responses.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Immunology
Cilian O. Maoldomhnaigh, Donal J. Cox, James J. Phelan, Morgane Mitermite, Dearbhla M. Murphy, Gina Leisching, Lorraine Thong, Seonadh M. O'Leary, Karl M. Gogan, Kate McQuaid, Amy M. Coleman, Stephen V. Gordon, Sharee A. Basdeo, Joseph Keane
Summary: In response to Mtb infection, macrophages upregulate glycolysis and effector functions to combat the bacteria, but the presence of lactate affects cellular metabolism immediately by decreasing glycolysis and increasing oxidative phosphorylation. This results in a reduced glycolytic shift and pro-inflammatory cytokine production, while enhancing the ability to kill Mtb through promoting autophagy.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Shufan Liang, Jiechao Ma, Hanlin Gong, Jun Shao, Jingwei Li, Yuejuan Zhan, Zhoufeng Wang, Chengdi Wang, Weimin Li
Summary: This article reviews the role of noncoding RNAs (ncRNAs) in Mycobacterium tuberculosis infection, highlighting their impact through immune response modulation, and their potential as biomarkers for tuberculosis diagnosis, drug resistance identification, treatment evaluation, and adverse drug reaction prediction.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Ying Zhou, Tianying Zhong, Wenjing Wei, Zhuhua Wu, Anping Yang, Ning Liu, Ming Wang, Xiaoli Zhang
Summary: The study characterized the impact of downregulating Mtsp17 on gene expression in Myobacterium smegmatis, revealing that Mtsp17 may act as an activator of desA1 and regulate the SigF regulon through the SigF regulatory pathway. This sheds light on the transcriptional regulation role of Mtsp17 and provides new insights into the molecular mechanisms of START family proteins.
Article
Microbiology
Marianne Martin, Angelique deVisch, Yves-Marie Boudehen, Philippe Barthe, Claude Gutierrez, Obolbek Turapov, Talip Aydogan, Laurene Heriaud, Jerome Gracy, Olivier Neyrolles, Galina V. Mukamolova, Francois Letourneur, Martin Cohen-Gonsaud
Summary: Mtb infection reprograms host metabolism, promoting its survival and spread. The secreted protein Rv1813c from Mtb manipulates host metabolism by targeting mitochondria, boosting ATP production and delaying cytochrome c release. This study reveals a novel class of mitochondrial effectors that may participate in host cell metabolic reprogramming and apoptosis control during Mtb infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Eddie M. Wampande, Stavroula K. Hatzios, Beatrice Achan, Ezekiel Mupere, Mary Nsereko, Harriet K. Mayanja, Kathleen Eisenach, W. Henry Boom, Sebastien Gagneux, Moses L. Joloba
BMC INFECTIOUS DISEASES
(2015)
Article
Biochemistry & Molecular Biology
Stavroula K. Hatzios, Soeren Abel, Julianne Martell, Troy Hubbard, Jumpei Sasabe, Diana Munera, Lars Clark, Daniel A. Bachovchin, Firdausi Qadri, Edward T. Ryan, Brigid M. Davis, Eranthie Weerapana, Matthew K. Waldor
NATURE CHEMICAL BIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Stavroula K. Hatzios, Anthony T. Iavarone, Carolyn R. Bertozzi
Article
Chemistry, Medicinal
Stavroula K. Hatzios, Michael W. Schelle, Gerald L. Newton, Kimberly M. Sogi, Cynthia M. Holsclaw, Robert C. Fahey, Carolyn R. Bertozzi
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2011)
Article
Biochemistry & Molecular Biology
Stavroula K. Hatzios, Michael W. Schelle, Cynthia M. Holsclaw, Christopher R. Behrens, Zsofia Botyanszki, Fiona L. Lin, Brian L. Carlson, Pawan Kumar, Julie A. Leary, Carolyn R. Bertozzi
JOURNAL OF BIOLOGICAL CHEMISTRY
(2009)
Article
Multidisciplinary Sciences
Stavroula K. Hatzios, Simon Ringgaard, Brigid M. Davis, Matthew K. Waldor
Article
Multidisciplinary Sciences
Stavroula K. Hatzios, Christina E. Baer, Tige R. Rustad, M. Sloan Siegrist, Jennifer M. Pang, Corrie Ortega, Tom Alber, Christoph Grundner, David R. Sherman, Carolyn R. Bertozzi
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2013)
Article
Multidisciplinary Sciences
Diana Munera, Jennifer M. Ritchie, Stavroula K. Hatzios, Rod Bronson, Gang Fang, Eric E. Schadt, Brigid M. Davis, Matthew K. Waldor
NATURE COMMUNICATIONS
(2014)
Article
Biochemistry & Molecular Biology
Matthew Howell, Daniel G. Dumitrescu, Lauren R. Blankenship, Darby Herkert, Stavroula K. Hatzios
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Review
Biochemistry & Molecular Biology
Brent R. Stockwell, Jose Pedro Friedmann Angeli, Hulya Bayir, Ashley I. Bush, Marcus Conrad, Scott J. Dixon, Simone Fulda, Sergio Gascon, Stavroula K. Hatzios, Valerian E. Kagan, Kay Noel, Xuejun Jiang, Andreas Linkermann, Maureen E. Murphy, Michael Overholtzer, Atsushi Oyagi, Gabriela C. Pagnussat, Jason Park, Qitao Ran, Craig S. Rosenfeld, Konstantin Salnikow, Daolin Tang, Frank M. Torti, Suzy V. Torti, Shinya Toyokuni, K. A. Woerpel, Donna D. Zhang