Article
Microbiology
Sandra Sanchez, Caroline M. Dunn, Daniel B. Kearns
Summary: This study identifies the lytic transglycosylase CwlQ as a critical enzyme for flagellum-dependent swarming motility in Gram-negative bacteria, while the lyase essential for flagellar insertion in Gram-positive bacteria remains unknown. Even when mutated in combination with other lyases related to motility, CwlQ mutation does not affect flagellar biosynthesis in Gram-positive bacteria.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Multidisciplinary Sciences
Shoichi Tachiyama, Kar L. Chan, Xiaolin Liu, Skander Hathroubi, Briana Peterson, Mohammad F. Khan, Karen M. Ottemann, Jun Liu, Anna Roujeinikova
Summary: The conserved protein FliL is an assembly factor of the Helicobacter pylori flagellar motor and colocalizes with the stator. Cryogenic electron tomography reconstructions reveal that FliL forms rings integrated with the MotAB units, and FliL-C shows structural similarities to scaffolding proteins. The findings provide insights into the assembly mechanism of the FliL rings and its potential role as a scaffold.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biotechnology & Applied Microbiology
Xiujuan Zhou, Bin Liu, Yanhong Liu, Chunlei Shi, Pina M. Fratamico, Lida Zhang, Dapeng Wang, Jianhua Zhang, Yan Cui, Ping Xu, Xianming Shi
Summary: The deletion of genes SC0368 and SC0595 in Salmonella Choleraesuis led to defects in motility and increased invasiveness in Caco-2 cells. Additionally, SC0368 deletion resulted in up-regulation of genes encoding cytochromes, enhancing bacterial proliferation. These findings suggest that these two genes play important roles in flagella biosynthesis, LPS synthesis, bacterial growth, and virulence within Salmonella C1 serogroup.
Article
Biochemistry & Molecular Biology
Sahar S. H. Tehrani, Pawel Mikulski, Izma Abdul-Zani, Joao F. Mata, Wojciech Siwek, Lars E. T. Jansen
Summary: Exposure of human cells to interferon-gamma (IFN gamma) results in a mitotically heritable yet reversible state called long-term transcriptional memory. We previously identified the clustered GBP genes as strongly primed by IFN gamma. Here, we discovered that in primed cells, both interferon-responsive transcription factors STAT1 and IRF1 target chromatin with accelerated kinetics upon re-exposure to IFN gamma, specifically at promotors of primed genes.
Article
Multidisciplinary Sciences
Linxiang Lan, Theodore Evan, Huafu Li, Aasia Hussain, E. Josue Ruiz, May Zaw Thin, Rute M. M. Ferreira, Hari Ps, Eva M. Riising, Yoh Zen, Jorge Almagro, Kevin W. Ng, Pablo Soro-Barrio, Jessica Nelson, Gabriela Koifman, Joana Carvalho, Emma L. Nye, Yulong He, Changhua Zhang, Anguraj Sadanandam, Axel Behrens
Summary: This study identifies BMP inhibitor GREM1 as a key regulator of cellular heterogeneity in pancreatic cancer. Continuous activity of GREM1 is required to maintain the stability of the epithelial PDAC subpopulations, while high activity of GREM1 can revert the mesenchymal fate of PDAC cells. By inhibiting the expression of epithelial-mesenchymal transition transcription factors, GREM1 restricts the epithelial-mesenchymal plasticity of pancreatic cancer cells.
Article
Biochemistry & Molecular Biology
Monica L. Bomber, Jing Wang, Qi Liu, Kelly R. Barnett, Hillary M. Layden, Emily Hodges, Kristy R. Stengel, Scott W. Hiebert
Summary: Genetic models indicated that SMARCA5 is essential for DNA-templated events and its degradation affects nucleosome repeat length and CTCF binding sites. The rapid degradation of SMARCA5 resulted in increased nucleosome repeat length and disruption of CTCF DNA binding. Our findings suggest that SMARCA5 is continuously required for maintaining nucleosomal spacing and controlling nucleosome repeat length.
Article
Microbiology
Qun Han, Shao-Feng Wang, Xin-Xin Qian, Lu Guo, Yi-Feng Shi, Rui He, Jun-Hua Yuan, Yan-Jie Hou, De-Feng Li
Summary: In E. coli and related species, the flagellar brake protein YcgR responds to increased levels of c-di-GMP and regulates bacterial swimming by decreasing flagellar rotation speed and biasing rotation direction. The target proteins involved and the role of motor proteins in this regulation are still unclear. This study found that YcgR interacts with both MotA and FliG, with enhanced affinities upon c-di-GMP binding. Furthermore, specific residues in YcgR were found to be necessary for binding to FliG. Mutations in these binding residues restored flagellar rotation speed and bias in wild-type cells. Thus, c-di-GMP-activated YcgR regulates flagellar rotation speed and direction through interactions with motor proteins MotA and FliG.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biology
Pavla Tumova, Lubos Voleman, Andreas Klingl, Eva Nohynkova, Gerhard Wanner, Pavel Dolezal
Summary: The presence of mitochondria is a distinguishing feature between prokaryotic and eukaryotic cells. Eukaryotes control mitochondrial inheritance mainly due to the presence of the genetic component, but little is known about the segregation of mitochondria to daughter cells during cell division. Anaerobic eukaryotic microbes evolved genomeless mitochondria-related organelles (MROs) providing a distinct mechanistic basis for maintenance of stable mitochondrial numbers.
Article
Oncology
Takaaki Yoshikawa, Akihisa Fukuda, Mayuki Omatsu, Mio Namikawa, Makoto Sono, Yuichi Fukunaga, Tomonori Masuda, Osamu Araki, Munemasa Nagao, Satoshi Ogawa, Kenji Masuo, Norihiro Goto, Yukiko Hiramatsu, Yu Muta, Motoyuki Tsuda, Takahisa Maruno, Yuki Nakanishi, Kenji Kawada, Shigeo Takaishi, Hiroshi Seno
Summary: Brg1 is crucial for intestinal TSCs in mice by inhibiting apoptosis and is critical for cell survival and stem cell features in human CRC cells, representing a new therapeutic target for human CRC.
JOURNAL OF PATHOLOGY
(2021)
Article
Multidisciplinary Sciences
Soumya Banerjee, Samuel Vernon, Wei Jiao, Ben Jiwon Choi, Evelyne Ruchti, Jamshid Asadzadeh, Olivier Burri, R. Steven Stowers, Brian D. McCabe
Summary: The decline of synaptic structures and functions during ageing can be mitigated by enhancing miniature neurotransmission, which is essential for maintaining and prolonging motor ability in adult organisms.
NATURE COMMUNICATIONS
(2021)
Article
Cell & Tissue Engineering
Morgan D. Zumbaugh, Ashley E. Geiger, Jing Luo, Zhengxing Shen, Hao Shi, David E. Gerrard
Summary: The study revealed that O-GlcNAcylation plays a critical role in the maintenance of muscle satellite cell health and function. Deficiency of OGT affects the proliferation, self-renewal, and repair capacity of SCs in injured muscle.
Article
Biochemistry & Molecular Biology
Anne Seifert, Hauke Drechsler, Julia Japtok, Till Korten, Stefan Diez, Andreas Hermann
Summary: Deficient intracellular transport is a common feature of neurodegenerative diseases. Mutations in the FUS gene are a common genetic cause of ALS, but the negative effect of FUS (P525L) on axonal transport is indirect and requires additional factors or mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Julian Haase, Richard Chen, Wesley M. Parker, Mary Kate Bonner, Lisa M. Jenkins, Alexander E. Kelly
Summary: The TFIIH complex plays a crucial role in maintaining chromosome compaction and condensin enrichment by dynamically altering the chromatin environment to facilitate condensin loading and condensin-dependent loop extrusion.
Article
Cell Biology
Jae Yeon Hwang, Huafeng Wang, Yonggang Lu, Masahito Ikawa, Jean-Ju Chung
Summary: In mammalian sperm cells, the regulation of spatiotemporal Ca2+ signaling relies on quadrilinear Ca2+ signaling nanodomains in the flagellar membrane. CatSpert, a C2 domain protein encoded by C2cd6, independently migrates to the flagella and serves as a major targeting component of the CatSper channel complex. CatSpert is essential for sperm hyperactivated motility and male fertility, and it targets the CatSper channel to the quadrilinear nanodomains in developing spermatids' flagella.
Review
Parasitology
Clare Halliday, Artur de Castro-Neto, Carolina L. Alcantara, Narcisa L. Cunha-e-Silva, Sue Vaughan, Jack D. Sunter
Summary: There are differences in morphology and function of the flagellar pockets among different trypanosomatids, with their life cycles and ecological niches likely influencing these differences.
TRENDS IN PARASITOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Joanna R. Brown, Cindi L. Schwartz, Johil. M. Heumann, Scott C. Dawson, Andreas Hoenger
JOURNAL OF STRUCTURAL BIOLOGY
(2016)
Article
Immunology
Jonathan K. Pham, Christopher Nosala, Erica Y. Scott, Kristofer F. Nguyen, Kari D. Hagen, Hannah N. Starcevich, Scott C. Dawson
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2017)
Article
Microbiology
N. R. Barash, C. Nosala, J. K. Pham, S. G. McInally, S. Gourguechon, B. McCarthy-Sinclair, S. C. Dawson
Review
Cell Biology
Christopher Nosala, Kari D. Hagen, Scott C. Dawson
TRENDS IN CELL BIOLOGY
(2018)
Article
Cell Biology
S. G. McInally, K. D. Hagen, C. Nosala, J. Williams, K. Nguyen, J. Booker, K. Jones, Scott C. Dawson
MOLECULAR BIOLOGY OF THE CELL
(2019)
Article
Biology
Shane G. McInally, Jane Kondev, Scott C. Dawson
Article
Biochemistry & Molecular Biology
Kuo-Fu Tseng, Keith J. Mickolajczyk, Guangxi Feng, Qingzhou Feng, Ethiene S. Kwok, Jesse Howe, Elisar J. Barbar, Scott C. Dawson, William O. Hancock, Weihong Qiu
Article
Cell Biology
Christopher Nosala, Kari D. Hagen, Nicholas Hilton, Tiffany M. Chase, Kelci Jones, Rita Loudermilk, Kristofer Nguyen, Scott C. Dawson
JOURNAL OF CELL SCIENCE
(2020)
Editorial Material
Parasitology
Shane G. McInally, Scott C. Dawson
Summary: Hirst et al. used an affinity-purification approach based on TOG domains to reconstitute and define the in vitro dynamics of blood-stage Plasmodium falciparum alpha beta-tubulin. This study provides a crucial reagent for studying parasite microtubule dynamics and evaluating the efficacy of anti-microtubule drugs throughout the complex parasite life cycle.
TRENDS IN PARASITOLOGY
(2022)
Article
Microbiology
Rebecca L. Mickol, Artemis S. Louyakis, H. Lynn Kee, Lisa K. Johnson, Scott C. Dawson, Katherine R. Hargreaves, Grayson L. Chadwick, Dianne K. Newman, Jared R. Leadbetter, C. Titus Brown
Summary: The genome sequence of Tenacibaculum mesophilum strain ECR, isolated from Trunk River at Falmouth, Massachusetts, was reported in this study. The isolation and sequencing were carried out as part of the 2016 and 2018 Microbial Diversity courses at the Marine Biological Laboratory in Woods Hole, Massachusetts.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Genetics & Heredity
Srijak Bhatnagar, Elise S. Cowley, Sebastian H. Kopf, Sherlynette Perez Castro, Sean Kearney, Scott C. Dawson, Kurt Hanselmann, S. Emil Ruff
ENVIRONMENTAL MICROBIOME
(2020)
Article
Microbiology
Rebecca E. Parales, Gaurav Sharma, Xiangsheng Zhang, Gabriel A. Subuyuj, Jordan T. Langner, Matthew E. Wright, Jayna L. Ditty, Scott C. Dawson
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2019)
Article
Microbiology
H. Lynn Kee, Irina V. Mikheyeva, Rebecca L. Mickol, Scott C. Dawson, Dianne K. Newman, Jared R. Leadbetter
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2019)
Article
Immunology
N. R. Barash, J. G. Maloney, S. M. Singer, S. C. Dawsona
INFECTION AND IMMUNITY
(2017)
Article
Microbiology
Christopher Nosala, Scott C. Dawson
CURRENT CLINICAL MICROBIOLOGY REPORTS
(2015)