Article
Optics
Jakob G. P. Lingg, Thomas S. Bischof, Bernardo A. Arus, Emily D. Cosco, Ellen M. Sletten, Christopher J. Rowlands, Oliver T. Bruns, Andriy Chmyrov
Summary: The development of fluorophores with photoemission beyond 1000 nm provides the opportunity to develop novel fluorescence microscopes sensitive to those wavelengths. Imaging at wavelengths beyond the visible spectrum enables imaging depths of hundreds of microns in intact tissue, making this attractive for volumetric imaging applications.
LASER & PHOTONICS REVIEWS
(2023)
Article
Plant Sciences
Keith E. Duncan, Kirk J. Czymmek, Ni Jiang, August C. Thies, Christopher N. Topp
Summary: This study presents technical advances in using lab-based X-ray microscopy for high-resolution 3D imaging of plant samples at multiple scales. Serial imaging and improved sample preparation methods allow for the generation of sub-micron 3D volumes co-registered with lower magnification scans, providing explicit contextual reference. This method bridges the imaging gap between light and electron microscopy and can be applied to various economically and scientifically important plant systems.
Article
Agronomy
Benjamin Cervantes-Romero, Paulino Perez-Rodriguez, Philippe Rott, Apolonio Valdez-Balero, Monica Osnaya-Gonzalez, Alejandrina Robledo-Paz, Camilo Hernandez-Juarez, Jose Crossa, Greta H. Rosas-Saito, Hilda V. Silva-Rojas
Summary: The study investigated the distribution of leaf scald disease in the main sugarcane producing states in Mexico, finding it present in 8 out of 14 states and 12 out of 31 fields. The causal agent Xanthomonas albilineans was identified and its pathogenicity confirmed through inoculation experiments. This highlights the importance of selecting resistant varieties in breeding programs to combat leaf scald disease.
Article
Virology
Fernando Clavijo-Coppens, Nicolas Ginet, Sophie Cesbron, Martial Briand, Marie-Agnes Jacques, Mireille Ansaldi
Summary: Xylella fastidiosa is an emerging plant pathogen that has caused recent epidemics in southern Europe, leading researchers to explore alternative treatments such as phage therapy to control its spread and cure infected crops. Through isolation and characterization of various phages, including two new phage species, scientists were able to propose new taxonomic elements and make significant progress in understanding and combatting this pathogen.
Article
Agricultural Engineering
Meenaa Chandrasekar, Jeana L. Collins, Sanaz Habibi, Rebecca G. Ong
Summary: The tissue-specific biochemical heterogeneities of lignocellulosic biomass and their effect on biomass deconstruction can be best understood through the combination of confocal laser scanning microscopy (CLSM) and immunohistochemistry. A custom polydimethylsiloxane (PDMS) microfluidic imaging reactor was developed to overcome the challenges of studying fragile plant materials. The results showed that less lignified parenchyma cell walls are more susceptible to pretreatment, while highly lignified protoxylem cell walls are the most resistant during enzymatic hydrolysis.
BIORESOURCE TECHNOLOGY
(2024)
Article
Food Science & Technology
Dian W. Ningtyas, Bhesh Bhandari, Sangeeta Prakash
Summary: This study explored the physical properties of plant-based cream emulsion using different plant proteins and homogenisation pressures. Increasing the homogenisation pressure and protein content led to smaller fat globule sizes but larger particle sizes in the emulsion, indicating the potential to produce stable plant-based cream that can replace dairy cream.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2021)
Article
Food Science & Technology
Liliana Perez-Lavalle, Antonio Valero, Manuel Cejudo-Gomez, Elena Carrasco
Summary: Fresh strawberries are popular and consumed frequently, but outbreaks caused by food-borne pathogens have been associated with their consumption. Salmonella enterica subsp. enterica serovar Thompson can form biofilms on the surface of strawberries during storage, which allows the bacteria to survive and spread. This is crucial for food safety.
Article
Engineering, Mechanical
M. Gaier, H-T Lin, Z. N. Farhat, K. P. Plucknett
Summary: This study demonstrates the feasibility of using a heat treatable binder phase for TiC-based cermets, showing a significant increase in hardness and scratch resistance with heat treatment. The research provides insights into the effects of heat treatments on stainless steels in metal ceramic composites.
Article
Plant Sciences
Bernd Zechmann, Stefan Moestl, Guenther Zellnig
Summary: Focused ion beam scanning electron microscopy (FIB-SEM) and serial sectioning transmission electron microscopy (ssTEM) are powerful tools for the 3D reconstruction and volumetric extraction of plant cells, and there are differences between the two methods.
Article
Biochemistry & Molecular Biology
Mariele Montanari, Sabrina Burattini, Caterina Ciacci, Patrizia Ambrogini, Silvia Carloni, Walter Balduini, Daniele Lopez, Giovanna Panza, Stefano Papa, Barbara Canonico
Summary: This study compared the automated-mechanical and enzymatic disaggregation methods for obtaining cell suspensions from various rat tissues. The results showed that these methods worked similarly in some tissues, but the enzyme-free Medimachine II protocol preserved lysosome and mitochondria labeling better, while the enzymatic gentle dissociation consistently induced a lower amount of intracellular ROS.
Article
Engineering, Mechanical
Wujun Yin, Fabien Briffod, Haoyu Hu, Takayuki Shiraiwa, Manabu Enoki
Summary: In this study, high-resolution digital image correlation and laser scanning confocal microscopy were used to investigate the deformation behaviors of polycrystalline austenitic stainless steels at the sub-grain scale. The results showed that slip and mechanical twinning activities occurred at the early stage of yielding and increased with the applied load. The presence of nanotwins hindered slip glide, resulting in twin-slip interaction and grain boundary strengthening. Furthermore, the observation of out-of-plane roughness provided insights into the significant deformation gradient near boundaries.
INTERNATIONAL JOURNAL OF PLASTICITY
(2023)
Article
Environmental Sciences
Qingwei Lin, Zhiwen Huai, Luqman Riaz, Xue Peng, Shishi Wang, Biyun Liu, Fei Yu, Jianmin Ma
Summary: This study investigated the accumulation potential of aluminum at the subcellular level and its ultrastructural toxicity in submerged plants. The results showed that severe structural and ultrastructural damage occurred when plants were exposed to aluminum concentrations of 0.6 mg/L or higher. At a concentration of 1.5 mg/L, aluminum could be transferred from the roots to other parts of the plant, with the aged leaves being the most receptive tissues.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Chemistry, Physical
Tianyu Yan, Xinyu Wang, Siting Liu, Dawei Fan, Xinyi Xu, Qi Zeng, Hui Xie, Xiaoli Yang, Shouping Zhu, Xiaopeng Ma, Zhen Yuan, Xueli Chen
Summary: A novel NIR-II CLSM system has been developed using inexpensive SiPM for multicolor biological imaging in vivo, achieving spatial resolution close to the diffraction limit and very low fluorescence detection sensitivity. Experiments in mice demonstrated comparable multicolor imaging performance in both NIR-I and NIR-II windows.
Article
Biochemistry & Molecular Biology
Nawar Sakr, Olga Glazova, Liudmila Shevkova, Nikita Onyanov, Samira Kaziakhmedova, Alena Shilova, Maria V. Vorontsova, Pavel Volchkov
Summary: Tissue autofluorescence in fixed tissue sections is a major issue in fluorescence microscopy. The intense intrinsic fluorescence emitted by the adrenal cortex interferes with fluorescent labels, resulting in poor-quality images. Various tissue treatment methods were evaluated for reducing autofluorescence, and TrueBlack (TM) Lipofuscin Autofluorescence Quencher and MaxBlock (TM) Autofluorescence Reducing Reagent Kit were found to be the most effective, reducing autofluorescence intensity by 89-93% and 90-95%, respectively. These treatments preserved specific fluorescence signals and tissue integrity, allowing reliable detection of fluorescent labels in the adrenal cortex tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Riccardo Fontana, Anna Caproni, Mariaconcetta Sicurella, Stefano Manfredini, Anna Baldisserotto, Peggy Marconi
Summary: In this study, Moringa oleifera leaf extracts were used to investigate their effects on the biofilm formation of X. campestris pv. campestris. The results showed that the extracts and individual phenols could inhibit the growth of biofilm on abiotic surfaces, and the activity of the whole phytocomplex was higher than that of individual phenols. Moreover, the extracts also showed significant effects on cabbage leaves in vivo, unblocking the xylem and suggesting potential for black rot control.