Article
Chemistry, Multidisciplinary
Tina Arndt, Urmimala Chatterjee, Olga Shilkova, Juanita Francis, Johan Lundkvist, Daniel Johansson, Benjamin Schmuck, Gabriele Greco, Asa Ekblad Nordberg, Yan Li, Lars U. Wahlberg, Maud Langton, Jan Johansson, Cecilia Goetherstroem, Anna Rising
Summary: A recombinant spider silk protein-derived hydrogel that forms rapidly at 37 degrees C is developed as a drug release system and tissue engineering scaffold. The diffusion rate and mechanical properties of the hydrogel can be adjusted by changing the protein concentration. Encapsulated stem cells show high survival and viability, and bioactive molecules can be easily included in the gel and maintain their activity.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Review
Cell & Tissue Engineering
Xiaotong Zhao, Qiong Li, Zhikun Guo, Zongjin Li
Summary: Stem cell therapy is recognized as a promising strategy for treating degenerative diseases, but low cell retention and survival rates limit its clinical applications. Emerging biomaterials that provide a supportive cellular microenvironment have optimized the therapeutic efficacy of stem cells in injured tissues. Advances in engineered microenvironment, through co-transplanting with synthetic and tissue-derived biomaterials, offer unprecedented opportunities for significant influences in tissue repair and regeneration.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Markus Rimann, Astrid Juengel, Sara Mousavi, Nicole Moeschlin, Maurizio Calcagni, Karin Wuertz-Kozak, Florian Brunner, Stefan Dudli, Oliver Distler, Christian Adlhart
Summary: This study demonstrated the suitability of two synthetic polymers in creating electrospun scaffolds for 3D skin cell models, with P(AN-MA) mats showing enhanced cell compatibility and infiltration compared to Pul/PVA/PAA mats.
Article
Nanoscience & Nanotechnology
Afroditi Nanou, Ivan Lorenzo-Moldero, Kyriakos D. Gazouleas, Barbara Cortese, Lorenzo Moroni
Summary: Cancer biology research is shifting towards innovative in vitro 3D culture models to better mimic in vivo cancer biology. This study evaluated a 3D engineered system using porous scaffolds with two different porosities and compared it to conventional 2D cell culture. The results showed that static seeding was the optimal method and cells cultured in 3D scaffolds exhibited a more dormant phenotype.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Medicine, Research & Experimental
Mengyuan Wang, Ying Li, Hongqiao Wang, Meng Li, Xiaomin Wang, Rongzhen Liu, Daijun Zhang, Wenhua Xu
Summary: Corneal epithelial defects and excessive wound healing can cause severe complications. Stem cells offer a promising solution for regenerating the corneal epithelium and treating severe corneal epithelial injury. Biological scaffolds such as the amniotic membrane, fibrin, and hydrogels provide the necessary signals for stem cell proliferation and differentiation. Researchers have evaluated these scaffolds as potential therapeutic interventions for corneal disorders, identifying both benefits and drawbacks. This study provides a comprehensive overview of the history and use of stem cells in corneal repair, focusing on biological scaffolds used in stem cell transplantation and innovative materials under investigation.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Materials Science, Ceramics
Jose Guilherme Neves, Daniel Navarro da Rocha, Camila Cristine Lopes, Rafael Maza Barbosa, Lorena Fernandes Ferreira, Cecilia Buzatto Westin, Angela Maria Moraes, Bruno Calsa, Milton Santamaria Jr, Lourenco Correr-Sobrinho, Americo Bortolazzo Correr
Summary: The aim of this study was to synthesize and characterize polymeric porous scaffolds associated with different calcium phosphates (CaP) and Mesenchymal Stem Cells (MSC) for regenerative dentistry application. The results showed that CaP improved the mechanical properties of scaffolds but decreased the cell viability. MSCs improved the inflammatory response after 60 days.
CERAMICS INTERNATIONAL
(2022)
Article
Chemistry, Applied
Sushma Kumari, Pritiranjan Mondal, Kaushik Chatterjee
Summary: This study demonstrates the excellent printability of photocurable methacrylate-kappa-carrageenan (MA-kappa-CA) in DLP 3D printing and its suitability for bioprinting highly complex 3D cell-laden scaffolds. Through thorough characterization of its physical and biological properties, this bioink has shown potential as a material for tissue scaffold fabrication.
CARBOHYDRATE POLYMERS
(2022)
Review
Engineering, Chemical
Leonidas Kandilogiannakis, Eirini Filidou, George Kolios, Vasilis Paspaliaris
Summary: Regenerative medicine uses stem cells for cell therapy to regenerate and restore damaged tissues and organs, and stem cell encapsulation is a possible solution to prevent host immune responses. Natural and synthetic hydrogels are used for stem cell encapsulation in regenerative medicine to protect cells and enhance their effectiveness.
Article
Polymer Science
Denisa-Maria Radulescu, Ionela Andreea Neacsu, Alexandru-Mihai Grumezescu, Ecaterina Andronescu
Summary: In recent years, the development and characterization of biomaterials for regenerative medicine and controlled release have posed significant challenges. Hydrogels are considered optimal applications in the biomedical field, particularly in tissue engineering. However, their delicate nature and low mechanical strength limit their use. Therefore, developing stronger and more stable hydrogels with increased biocompatibility is essential. Natural and synthetic polymers both have limitations, but the development of hydrogels based on mixtures of these materials can optimize their properties. Incorporating different nanoparticles can improve stability and yield various biological effects. Essential oils and drug molecules can facilitate desired biological effects and even produce a synergistic effect. The main purpose of this study is to identify the key properties required to develop sustainable polymeric scaffolds for tissue engineering without adverse environmental effects.
Article
Biochemistry & Molecular Biology
Michal Halperin-Sternfeld, Gal Netanel Liberman, Raha Kannan, Francesca Netti, Peter X. Ma, Shoshana Malis Arad, Lihi Adler-Abramovich
Summary: Sulfated polysaccharides from red marine microalgae, when combined with FmocFF peptide hydrogelator, form transparent and stable nanofibrous hydrogels. The mechanical properties and sustained release capabilities of these composite hydrogels can be tuned by changing the hydrogel concentration. This study demonstrates the potential of these hydrogels for tissue engineering and cell differentiation.
Review
Cell Biology
Jianjun Li, Yufan Liu, Yijie Zhang, Bin Yao, Enhejirigala, Zhao Li, Wei Song, Yuzhen Wang, Xianlan Duan, Xingyu Yuan, Xiaobing Fu, Sha Huang
Summary: This review emphasizes the importance of understanding the biophysical and biochemical cues in guiding mesenchymal stem cell (MSC) behaviors, as well as integrating these cues into biomaterials to enhance the prospects of MSC applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ozge Karabiyik Acar, Seden Bedir, A. Basak Kayitmazer, Gamze Torun Kose
Summary: This study introduces a novel three-dimensional coacervate scaffold encapsulating bone marrow mesenchymal stem cells, with an interconnected structure promoting cell-cell and cell-matrix interactions. The scaffold demonstrates remarkable cellular viability under chondrogenic induction, and shows elevated expression levels of chondrogenic markers.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Engineering, Biomedical
Gyeong Min Lee, Se-jeong Kim, Eun Mi Kim, Eunhyung Kim, Sangmin Lee, Eunjin Lee, Hee Ho Park, Heungsoo Shin
Summary: A composite hydrogel incorporating epigallocatechin-gallate (EGCG)-coated nanofiber particles (EF) was developed. Compared to traditional GelMA hydrogel, this composite hydrogel showed significant improvements in cell viability, intracellular oxidative stress, and cell spreading and migration ability.
ACTA BIOMATERIALIA
(2022)
Article
Polymer Science
Yingjie He, Tao Jiang, Chuang Li, Cong Zhou, Guichun Yang, Junqi Nie, Feiyi Wang, Cuifen Lu, Dan Yin, Xiaofan Yang, Zhenbing Chen
Summary: Tissue engineering provides therapeutic strategies for organ and tissue loss or failure. Hydrogels, as a three-dimensional biomaterial that mimics the extracellular matrix in tissue engineering, have been widely studied and used as cell scaffolds. In this study, POSS-PEG/PEG hydrogels were developed through click reaction between a star-shaped cross-linker and poly(ethylene glycol)-dithiol. The hydrogels showed a large water content, improved mechanical strength, controllable degradation rate, excellent cell compatibility, and porous internal structure, making them promising as cell scaffold materials.
POLYMER DEGRADATION AND STABILITY
(2023)
Article
Engineering, Biomedical
Murat Taner Vurat, Mahmut Parmaksiz, Ayse Eser Elcin, Yasar Murat Elcin
Summary: This study investigated the feasibility of using solubilized DBM and nHAp-incorporated DBM hydrogels as encapsulation matrix for BM-MSCs. The hydrogels were found to be cytocompatible and hemocompatible, with sufficient mechanical stability at physiological temperature. Preliminary in vivo study showed that DBM/nHAp had higher osteogenicity, and BM-MSC encapsulated DBM/nHAp showed predominant bone-like tissue formation in rat ectopic sites.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Engineering, Manufacturing
Qingxi Hu, Jian Cui, Haiguang Zhang, Suihong Liu, Murugan Ramalingam
Summary: In this study, a 5 + 1-axis 3D printing platform was developed to produce support-free intestinal fistula stents. A dynamic slicing algorithm was used to process a 3D model of the target stent shape, resulting in improved printing efficiency, superior stent surface properties and structure, ideal elasticity, and mechanical strength to meet the mechanical requirements of the human body. Static simulations demonstrated the importance of axial printing techniques, while the stent itself showed excellent biocompatibility.
3D PRINTING AND ADDITIVE MANUFACTURING
(2023)
Review
Multidisciplinary Sciences
T. S. Sampath Kumar, K. Madhumathi, R. Jayasree
Summary: This article provides insight into the recent medical applications of eggshell-based calcium phosphate bioceramics. It discusses the advantages of using natural sources such as eggshell, bovine bone, and fish bone for bioceramics, including optimal composition and morphology. The article highlights the potential of eggshell-derived calcium phosphates for bone regenerative applications and summarizes the development of eggshell-derived nanocarriers for drug and protein delivery. It also emphasizes the role of eggshell calcium phosphate precursors in improving the material and biological properties of bone cement formulations, as well as the advantages of eggshell-based scaffolds in tissue engineering and regenerative medicine.
JOURNAL OF THE INDIAN INSTITUTE OF SCIENCE
(2022)
Article
Engineering, Biomedical
Deepti Rana, Ajoy Kandar, Nasim Salehi-Nik, Ilyas Inci, Bart Koopman, Jeroen Rouwkema
Summary: This paper presents an aptamer-based programmable growth factor (GF) delivery platform that allows spatiotemporal control over vascular endothelial GF (VEGF) availability. The platform can sequester and release VEGF in a localized and time-dependent manner, and it has shown the ability to selectively guide cell responses and manipulate microvascular network formation within a 3D microenvironment.
BIOACTIVE MATERIALS
(2022)
Review
Chemistry, Physical
Mohammed S. Alqahtani, Mohamed Abbas, Mohammed Abdulmuqeet, Abdullah S. Alqahtani, Mohammad Y. Alshahrani, Abdullah Alsabaani, Murugan Ramalingam
Summary: The COVID-19 pandemic has led to paradigm shifts in various aspects of society, with long-term consequences that are yet to be fully understood. This review paper focuses on the use of nanomaterials for the prevention, detection, and diagnosis of the SARS-CoV-2 virus. Nanomaterials, such as nanoparticles, quantum dots, nanowires, and carbon nanotubes, offer unique properties that make them ideal for creating sensitive sensors. Scientists are exploring portable and easy-to-use COVID-19 detection tools using nanomaterials.
Review
Metallurgy & Metallurgical Engineering
Manas Ranjan Sahu, T. S. Sampath Kumar, Uday Chakkingal
Summary: Binary Mg-Ca alloys are receiving increasing attention as temporary implant materials due to their excellent biocompatibility, biodegradability, and good mechanical properties. However, their high degradation rates, hydrogen gas release, and rapid loss in mechanical properties in the human physiological environment limit their applications. Researchers have addressed these issues through control of Ca content, thermo-mechanical processing, and surface coatings.
JOURNAL OF MAGNESIUM AND ALLOYS
(2022)
Article
Chemistry, Analytical
Xiangyi Su, Yile Fang, Haoran Liu, Yue Wang, Minjie Ji, Zhu Chen, Hui Chen, Song Li, Yan Deng, Lian Jin, Yuanying Zhang, Murugan Ramalingam, Nongyue He
Summary: This study designs a detection-service-mobile three-terminal system for the control of diagnostic instruments and comprehensive data management. By using machine learning to classify enlarged curves and combining with a geographic information system, the visual display of nucleic acid amplification detection results and early warning of infectious diseases are achieved.
Review
Pharmacology & Pharmacy
Serge Ostrovidov, Murugan Ramalingam, Hojae Bae, Gorka Orive, Toshinori Fujie, Xuetao Shi, Hirokazu Kaji
Summary: With the advances in skeletal muscle tissue engineering, new platforms have emerged for biology studies, disease modeling, and drug testing. The authors review the latest advances in in vitro models of engineered skeletal muscle tissues used for drug testing, focusing on four main cell culture techniques: well plate cultures, microfluidics, organoids, and bioprinted constructs. More developments are expected to increase the validation and use of these models in drug testing.
EXPERT OPINION ON DRUG DISCOVERY
(2023)
Article
Engineering, Biomedical
Vimal Kumar Dewangan, T. S. Sampath Kumar, Mukesh Doble, Viju Daniel Varghese
Summary: Antibiotic-loaded bioactive bone substitutes are widely used to treat various orthopedic diseases and prevent post implantation infection. This study investigated the fabrication of mannitol incorporated injectable synthetic and eggshell derived apatitic bone cements loaded with antibiotics, and found that they showed controlled antibiotic release, acceptable compressive strength, biodegradability, antibacterial activity, and cytocompatibility. These antibiotic-loaded bone cements can be potential injectable bone substitutes to prevent post-operative implant associated and other bone infections.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Multidisciplinary Sciences
Mareeswari Paramasivan, T. S. Sampath Kumar, Hemalatha Kanniyappan, Vignesh Muthuvijayan, T. S. Chandra
Summary: Biomimicry has been adopted in bioceramics due to its functional advantages. In this study, hydroxyapatite synthesized via biomimetic approach was substituted with individual and multiple cations. The substituted hydroxyapatite showed decreased crystallinity, phase purity, and altered morphology. The co-substituted hydroxyapatite with 1% Sr, Zn, and Mg showed promising biocompatibility.
SCIENTIFIC REPORTS
(2023)
Article
Materials Science, Multidisciplinary
Yun Shi, Ting Deng, Yu Peng, Zugan Qin, Murugan Ramalingam, Yang Pan, Cheng Chen, Feng Zhao, Lijia Cheng, Juan Liu
Summary: The objective of this study was to enhance the biological activity of PEEK implants through sulfonation treatment. The results showed that SPEEK2 exhibited superior biocompatibility and osteogenic activity compared to other groups.
Article
Engineering, Biomedical
Deepti Rana, Prasanna Padmanaban, Malin Becker, Fabian Stein, Jeroen Leijten, Bart Koopman, Jeroen Rouwkema
Summary: Given the need for control over evolving biochemical cues within synthetic matrices, this study focused on the incorporation of VEGF165 specific aptamers into cell-instructive polymer networks to regulate VEGF bioactivity. Bicomponent micropatterns were fabricated via photopatterning and demonstrated the retention of aptamers, molecular recognition, and VEGF sequestration. The study also revealed the ability of aptamer-tethered VEGF to spatiotemporally regulate vascular morphogenesis.
MATERIALS TODAY BIO
(2023)
Article
Nanoscience & Nanotechnology
Wanru Sun, Huifang Xiao, Jiazhi Zhu, Zhaokun Hao, Jian Sun, Deqiang Wang, Xin Wang, Murugan Ramalingam, Shuyang Xie, Ranran Wang
Summary: Sonodynamic therapy (SDT) and chemotherapy have been combined using a tumor microenvironment-responsive biodegradable nanoplatform to treat hypoxic tumors, resulting in improved therapeutic efficacy. The nanoplatform encapsulates manganese oxide (MnO2) into silica nanoparticles and incorporates a sonosensitizer (Ce6) and a chemotherapeutic drug (DOX), which are released in response to glutathione (GSH) and catalyzed by MnO2 to generate oxygen (O-2), alleviating tumor hypoxia. Ultrasonic stimulation further enhances chemotherapy drug release and cellular uptake, while also downregulating P-gp protein expression to reduce drug efflux. This study demonstrates the potential of the nanoplatform for efficient chemotherapy and SDT synergistic therapy for hypoxic tumors.
ACS APPLIED BIO MATERIALS
(2023)
Article
Agricultural Engineering
Xiyue Li, Yanjing Liu, Yaping Huang, Fuming Wang, Xuyang Feng, Botian Zhu, Murugan Ramalingam, Ying Wang
Summary: This study fabricated nanofiber membranes using pullulan and pectin substrates and evaluated their digestion characteristics in simulated gastrointestinal fluids. The results showed that the pullulan/pectin nanofibers had optimal morphology and mechanical properties, and were degradable by enzymes in intestinal fluid, indicating potential for targeted drug or bioactive delivery.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Meeting Abstract
Cell & Tissue Engineering
Murugan Ramalingam, Guang-Zhen Jin, Deepti Rana, Jung-Hwan Lee, Hae-Won Kim
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Deepti Rana, Vasileios D. Trikalitis, Vincent R. Rangel, Prasanna Padmanaban, Ajoy Kandar, Jeroen Rouwkema
TISSUE ENGINEERING PART A
(2022)
