Journal
CARBOHYDRATE POLYMERS
Volume 290, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2022.119508
Keywords
Digital light processing; Carbohydrates; Hydrogels; 3D bioprinting; Tissue engineering
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Funding
- National Postdoctoral fellowship (NPDF) from the Science and Engineering Research Board (SERB) , Ministry of Science and Technology, Government of India [PDF/2019/002997]
- SERB [IPA/2020/000025]
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This study demonstrates the excellent printability of photocurable methacrylate-kappa-carrageenan (MA-kappa-CA) in DLP 3D printing and its suitability for bioprinting highly complex 3D cell-laden scaffolds. Through thorough characterization of its physical and biological properties, this bioink has shown potential as a material for tissue scaffold fabrication.
The demand to regenerate biological tissues and organs in patients as an alternative to transplants has motivated the tissue engineering field. Digital light processing (DLP)-based three-dimensional (3D) bioprinting technology enables the rapid fabrication of complex 3D cell-laden scaffolds for tissue engineering applications. Herein, we demonstrate the outstanding printability of photocurable methacrylate-kappa-carrageenan (MA-kappa-CA) using DLP 3D printing. 3D printed hydrogels with varying concentrations (1-5% w/v) of MA-kappa-CA were thoroughly characterized for their swelling, degradation, mechanical, and rheological properties, and suitability for bioprinting with living cells. Viscosity and shear thinning behavior of MA-kappa-CA faithfully recapitulate the biomechanical properties of soft human tissues. Encapsulated NIH-3T3 cells show high viability and good proliferation over several days. Furthermore, highly complex 3D hydrogel scaffolds of MA-kappa-CA were printed to recapitulate the biological complexity of tissues and organs. This work presents a polysaccharide bioink for preparing tissue scaffolds by DLP 3D bioprinting.
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