Parallel use of shake flask and microtiter plate online measuring devices (RAMOS and BioLector) reduces the number of experiments in laboratory-scale stirred tank bioreactors

Quantifying the sensitivity of G. oxydans ATCC 621H and DSM 3504 to osmotic stress triggered by soluble buffers

(2015) B. Luchterhand et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Fed-batch operation in special microtiter plates: a new method for screening under production conditions

(2014) Anja Wilming et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Pitfalls in optical on-line monitoring for high-throughput screening of microbial systems

(2014) Martin Kunze et al.   Microbial Cell Factories

Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase

(2014) Johannes Hemmerich et al.   Microbial Cell Factories

Scale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulation

(2013) Mauricio A. Trujillo-Roldán et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Evidence for a Key Role of Cytochrome bo3 Oxidase in Respiratory Energy Metabolism of Gluconobacter oxydans

(2013) J. Richhardt et al.   JOURNAL OF BACTERIOLOGY

Investigation of the physiological response to oxygen limited process conditions of Pichia pastoris Mut+ strain using a two-compartment scale-down system

(2013) Bettina Lorantfy et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Dialysis shake flask for effective screening in fed-batch mode

(2012) Cornelia Bähr et al.   BIOCHEMICAL ENGINEERING JOURNAL

A system of miniaturized stirred bioreactors for parallel continuous cultivation of yeast with online measurement of dissolved oxygen and off-gas

(2012) Tobias Klein et al.   BIOTECHNOLOGY AND BIOENGINEERING

Short-chain fluorescent tryptophan tags for on-line detection of functional recombinant proteins

(2012) Eva-Maria Siepert et al.   BMC BIOTECHNOLOGY

An automated workflow for enhancing microbial bioprocess optimization on a novel microbioreactor platform

(2012) Peter Rohe et al.   Microbial Cell Factories

Development of a modified Respiration Activity Monitoring System for accurate and highly resolved measurement of respiration activity in shake flask fermentations

(2012) Sven Hansen et al.   Journal of Biological Engineering

Simple defined autoinduction medium for high-level recombinant protein production using T7-based Escherichia coli expression systems

(2011) Zhaopeng Li et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Predictive tool for recombinant protein production in Escherichia coli Shake-Flask cultures using an on-line monitoring system

(2011) Martin Kunze et al.   BIOTECHNOLOGY PROGRESS

Utilizing high-throughput experimentation to enhance specific productivity of an E.coli T7 expression system by phosphate limitation

(2011) Robert Huber et al.   BMC BIOTECHNOLOGY

High-throughput protein expression screening and purification in Escherichia coli

(2011) Renaud Vincentelli et al.   METHODS

Reducing conditions are the key for efficient production of active ribonuclease inhibitor in Escherichia coli

(2011) Juozas Šiurkus et al.   Microbial Cell Factories

Heterologous production of active ribonuclease inhibitor in Escherichia coli by redox state control and chaperonin coexpression

(2011) Juozas Šiurkus et al.   Microbial Cell Factories

Glucose-limited high cell density cultivations from small to pilot plant scale using an enzyme-controlled glucose delivery system

(2011) Julia Glazyrina et al.   New Biotechnology

Flavin Mononucleotide-Based Fluorescent Reporter Proteins Outperform Green Fluorescent Protein-Like Proteins as Quantitative In Vivo Real-Time Reporters

(2010) T. Drepper et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A review of advanced small-scale parallel bioreactor technology for accelerated process development: Current state and future need

(2010) Rachel Bareither et al.   BIOTECHNOLOGY PROGRESS

Replication methods and tools in high-throughput cultivation processes - recognizing potential variations of growth and product formation by on-line monitoring

(2010) Robert Huber et al.   BMC BIOTECHNOLOGY

Bioprocess scale-up: quest for the parameters to be used as criterion to move from microreactors to lab-scale

(2010) Marco P. C. Marques et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Bioprocess Control in Microscale: Scalable Fermentations in Disposable and User-Friendly Microfluidic Systems

(2010) Matthias Funke et al.   Microbial Cell Factories

Novel approach of high cell density recombinant bioprocess development: Optimisation and scale-up from microlitre to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures

(2010) Juozas Šiurkus et al.   Microbial Cell Factories

A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures

(2010) Mirja Krause et al.   Microbial Cell Factories

Equalizing growth in high-throughput small scale cultivations via precultures operated in fed-batch mode

(2009) Robert Huber et al.   BIOTECHNOLOGY AND BIOENGINEERING

Plasmid-encoded protein: The principal factor in the “metabolic burden” associated with recombinant bacteria

(2009) William E. Bentley et al.   BIOTECHNOLOGY AND BIOENGINEERING

Asymmetric division ofHansenula polymorphareflected by a drop of light scatter intensity measured in batch microtiter plate cultivations at phosphate limitation

(2009) Kirsten Kottmeier et al.   BIOTECHNOLOGY AND BIOENGINEERING

High-throughput screening ofHansenula polymorphaclones in the batch compared with the controlled-release fed-batch mode on a small scale

(2009) Marco Scheidle et al.   FEMS YEAST RESEARCH

Robo-Lector – a novel platform for automated high-throughput cultivations in microtiter plates with high information content

(2009) Robert Huber et al.   Microbial Cell Factories

Scale-up from microtiter plate to laboratory fermenter: evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha fermentations

(2009) Frank Kensy et al.   Microbial Cell Factories

Validation of a high-throughput fermentation system based on online monitoring of biomass and fluorescence in continuously shaken microtiter plates

(2009) Frank Kensy et al.   Microbial Cell Factories

LOVely enzymes - towards engineering light-controllable biocatalysts

(2009) Ulrich Krauss et al.   Microbial Biotechnology

Quantification of power consumption and oxygen transfer characteristics of a stirred miniature bioreactor for predictive fermentation scale-up

(2008) N.K. Gill et al.   BIOTECHNOLOGY AND BIOENGINEERING

Respiration activity monitoring system (RAMOS), an efficient tool to study the influence of the oxygen transfer rate on the synthesis of lipopeptide by Bacillus subtilis ATCC6633

(2008) J.S. Guez et al.   JOURNAL OF BIOTECHNOLOGY

Enzyme controlled glucose auto-delivery for high cell density cultivations in microplates and shake flasks

(2008) Johanna Panula-Perälä et al.   Microbial Cell Factories

Design and characterisation of a miniature stirred bioreactor system for parallel microbial fermentations

(2007) N.K. Gill et al.   BIOCHEMICAL ENGINEERING JOURNAL

Scale-up ofEscherichia coli growth and recombinant protein expression conditions from microwell to laboratory and pilot scale based on matchedkLa

(2007) R.S. Islam et al.   BIOTECHNOLOGY AND BIOENGINEERING

Enhanced Bacterial Protein Expression During Auto-Induction Obtained by Alteration of Lac Repressor Dosage and Medium Composition

(2007) Paul G. Blommel et al.   BIOTECHNOLOGY PROGRESS