Article
Biotechnology & Applied Microbiology
Beyza Karacaoglu, Benan Inan, Didem Balkanli Ozcimen
Summary: The commercialization of high value-added bioactive compounds from microalgae faces various challenges, such as controlling parameters for process optimization and high production costs. Microfluidic devices are increasingly used in microalgal bioprocess studies due to their unique features, including small scale, controlled parameters, and high throughput capabilities. These devices outperform conventional techniques in terms of functions and analysis of small samples. This review discusses the diverse microfluidic platforms for microalgal processes, including cultivation, purification, separation, sorting, and high-throughput screening.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Biomedical
Lei Shao, Bingchu Pan, Ruxia Hou, Yuan Jin, Yudong Yao
Summary: This study developed a user-friendly microfluidic device to produce hydrogel microspheres using sharp needles. By modifying the device, heterogeneous microspheres could be easily manufactured. The GelMA microspheres showed high bioactivity and flexibility, making them suitable for cell culture and tissue construction.
Article
Chemistry, Analytical
Woohyuk Kim, Beomseok Cha, Jessie S. Jeon, Jinsoo Park
Summary: We have developed an acoustofluidic method for dynamically controlling the chemical concentration (CC) within picoliterscale droplets at high-throughput production. The method involves rapidly mixing two fluids using acoustic streaming flow-induced micro-vortices, and precisely controlling the mixing index through modulation of the wave amplitude. We have demonstrated the applicability of this method for producing droplet libraries with tunable CCs, which is promising for various droplet microfluidic applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemical Research Methods
Jaewook Ryu, Junhyeong Kim, Ki-Ho Han
Summary: A disposable droplet generation microfluidic device (dDrop-Chip) is introduced in this study, which can control both droplet size and production rate in real time. The dDrop-Chip consists of a reusable sensing substrate and a disposable microchannel and integrates a droplet detector and a flow sensor on-chip. Experimental results demonstrate that the dDrop-Chip consistently generates monodisperse droplets with controlled size and production rate, making it a reliable and cost-effective technique for droplet-based applications.
Review
Biochemical Research Methods
Sarah Taeuber, Alexander Gruenberger
Summary: Performance losses commonly occur during the scaling-up of bioprocesses, caused by concentration gradients in bioreactors. Scale-down bioreactors are used to analyze large-scale conditions and predict the successful transfer of bioprocesses from lab to industry. Microfluidic single-cell cultivation (MSCC) systems provide the opportunity to understand cellular processes at a single-cell level. However, current MSCC systems have limited cultivation parameters that do not represent bioprocess-relevant environmental conditions.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Engineering, Electrical & Electronic
Seungsik Moon, Namyoon Lee, Youngjoo Lee
Summary: In order to support large-scale multiuser multiple-input multiple-output systems and achieve their capacity, power allocation and user selection along with precoding need to be considered. This paper introduces a scalable MU-MIMO precoding processor that solves the joint optimization problem for precoding, power allocation, and user selection. The proposed processor is capable of solving the state-of-the-art joint optimization problem in only 0.51ms for the 64x64 large-scale MU-MIMO configuration.
IEEE TRANSACTIONS ON CIRCUITS AND SYSTEMS I-REGULAR PAPERS
(2023)
Article
Computer Science, Hardware & Architecture
Zhi Liu, Cheng Zhan, Ying Cui, Celimuge Wu, Han Hu
Summary: This article introduces a scalable aerial computing solution that can improve overall computing performance by accommodating computation tasks of multiple quality levels. Additionally, a cooperative computing solution called UAVideo is proposed to minimize video streaming time.
IEEE WIRELESS COMMUNICATIONS
(2021)
Article
Engineering, Electrical & Electronic
Shinsaku Izumi, Kensuke Nishijima, Xin Xin
Summary: This paper discusses the scalable control of power systems, focusing on the constraint that all generators have the same controller. By designing one controller for all generators, time and effort in constructing large-scale power systems are reduced. The controllers presented aim to stabilize the equilibrium point of the feedback system and improve time response performance.
ELECTRICAL ENGINEERING
(2022)
Article
Chemistry, Physical
Xiaoyu Shi, Junyu Chang, Jieqiong Qin, Hanqing Liu, Xiong Zhang, Yanwei Ma, Jian He, Xiujian Chou, Liang Feng, Zhong-Shuai Wu, Xinhe Bao
Summary: This study demonstrates a scalable construction method for self-powered integrated systems, utilizing various materials and strategies to achieve high integration and unique flexibility, while showing excellent performance in gas detection.
Article
Computer Science, Information Systems
Anlin Xu, Ping Li
Summary: This paper introduces a microfluidic device based on a segmented temperature sensor, which can achieve segmented temperature measurement and control of the solution in microchannels. The device has the advantages of simple structure, low cost, and convenient operation.
MOBILE INFORMATION SYSTEMS
(2021)
Article
Biochemical Research Methods
Roberto Maria Pellegrino, Matteo Giulietti, Husam B. R. Alabed, Sandra Buratta, Lorena Urbanelli, Francesco Piva, Carla Emiliani
Summary: LC/MS-based analysis techniques combined with specialized lipid tools allow for the qualitative and quantitative determination of thousands of lipid molecules. This article introduces a new lipidomic tool called LipidOne, which can analyze the qualitative and quantitative changes in lipid building blocks among all detected lipid classes and experimental groups. This tool enables the linking of differences in lipid building blocks to the activity of specific classes of enzymes, transcripts, and genes.
Article
Engineering, Electrical & Electronic
Xiaohui Hu, Chen Peng, Song Yang, Hao Shen
Summary: A novel mode-dependent scalable control strategy is proposed for large-scale networked systems. By constructing an arbitrary topology, the restrictions on the coupling structure of subsystems are removed. The derived sufficient conditions enable each subsystem to control the coupled subsystems using its own dynamics and limited information. Simulation results on a coupled microgrid validate the effectiveness of the proposed approach.
IEEE TRANSACTIONS ON CIRCUITS AND SYSTEMS II-EXPRESS BRIEFS
(2023)
Article
Engineering, Electrical & Electronic
Anastasios Papazafeiropoulos, Emil Bjornson, Pandelis Kourtessis, Symeon Chatzinotas, John M. Senior
Summary: Standard cell-free massive mMIMO systems are promising for meeting the high data rate demands of 5G networks. However, hardware impairments can significantly impact system performance and need to be properly evaluated and optimized. For SCF mMIMO systems, the effects of hardware impairments on transmission quality and performance need to be considered.
IEEE TRANSACTIONS ON VEHICULAR TECHNOLOGY
(2021)
Article
Automation & Control Systems
Andrea Carron, Francesco Seccamonte, Claudio Ruch, Emilio Frazzoli, Melanie N. Zeilinger
Summary: Technological advances in self-driving vehicles will soon enable the implementation of large-scale mobility-on-demand systems. The proposed discrete-time model for an autonomous MoD system is well-posed and suitable for model predictive control. The performance of the scheme in a multiagent transport simulation demonstrates superior service levels compared to existing rebalancing schemes.
IEEE TRANSACTIONS ON CONTROL SYSTEMS TECHNOLOGY
(2021)
Article
Engineering, Electrical & Electronic
Shuaifei Chen, Jiayi Zhang, Emil Bjornson, Jing Zhang, Bo Ai
Summary: This paper proposes a new framework for structured massive access in cell-free massive MIMO systems, which improves system performance through specific algorithms and strategies. The results show that this framework provides high spectral efficiency while ensuring user fairness.
IEEE JOURNAL ON SELECTED AREAS IN COMMUNICATIONS
(2021)
Article
Energy & Fuels
Anne Lunze, Benedikt Heyman, Yosra Chammakhi, Maria Eichhorn, Jochen Buechs, Nico Anders, Antje C. Spiess
Summary: The study successfully demonstrated the technical suitability of Silphium perfoliatum as a feedstock for a liquid hot water-based biorefinery process through experimental comparison and analysis. It also showed the potential application value of Silphium perfoliatum in the biorefinery process.
BIOENERGY RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Marcel Mann, Katharina Miebach, Jochen Buechs
Summary: Syngas fermentation can reduce greenhouse gas emissions, but detailed understanding on the impact of CO concentration on microbial fermentation is still lacking; a newly developed online measurement setup for DCOT provides reliable results in continuous stirred tank reactor fermentation experiments.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Marcel Mann, Aline Hueser, Benjamin Schick, Robert Dinger, Katharina Miebach, Jochen Buechs
Summary: Syngas fermentation technology shows great potential for emission reduction in the future, but further research is needed on key parameters such as substrate toxicity and gas transfer rates. The newly developed online monitoring device can facilitate rapid process development by measuring crucial parameters in real time.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Tobias Habicher, Tobias Klein, Jacqueline Becker, Andreas Daub, Jochen Buechs
Summary: The study successfully established a glucose-limited fed-batch screening process for protease production using a fed-batch microtiter plate, demonstrating its effectiveness at microtiter plate scale. This method helps increase the precision of identifying improved protease producing B. licheniformis strains.
MICROBIAL CELL FACTORIES
(2021)
Review
Biochemistry & Molecular Biology
Nick Wierckx, Katharina Miebach, Nina Ihling, Kai P. Hussnaetter, Jochen Buechs, Kerstin Schipper
Summary: Fungi of the Ustilaginaceae family are known for their pathogenic role in causing smut disease on plants, but they also have potential as natural producers of value-added substances. Among them, Ustilago maydis has emerged as a versatile model organism for both fundamental research and applied biotechnology. Advances in genetic engineering and process development have been utilized to enhance production yields and product specificity, making Ustilaginaceae fungi promising candidates for future biotech cell factories.
MICROBIAL CELL FACTORIES-BOOK
(2021)
Article
Plant Sciences
Judith Loogen, Andre Mueller, Arne Balzer, Sophie Weber, Kathrin Schmitz, Roxanne Krug, Ulrich Schaffrath, Jorg Pietruszk, Uwe Conrath, Jochen Buechs
Summary: This study introduces a modified mu RAMOS device for analyzing plant seedlings. The device was used to validate the suitability for identifying novel PrimACs in Arabidopsis thaliana, with the results showing Tyr020 as a likely PrimAC. The difference in OTR between primed and unprimed plants after elicitation with flg22 suggests that the mu RAMOS device can be reliably used for screening PrimACs in plant seedlings.
Article
Chemistry, Multidisciplinary
Marcel Gausmann, Christian Kocks, Johannes Pastoors, Jochen Buchs, Nick Wierckx, Andreas Jupke
Summary: This work presents a biobased production route for itaconic acid, featuring low pH value fermentation, reactive extraction, and electrochemical product recovery, resulting in a purity of more than 99% recovery of itaconic acid in crystalline form from the fermentation broth. The proposed downstream process can efficiently recover itaconic acid while eliminating the generation of waste salts, operating with electricity costs lower than sulfuric acid and sodium hydroxide typically required for pH-swing steps.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Nina Ihling, Lara Pauline Munkler, Christoph Berg, Britta Reichenbaecher, Johannes Wirth, Dietmar Lang, Roland Wagner, Jochen Buechs
Summary: The study utilized the Respiration Activity Monitoring System to monitor the oxygen transfer rate (OTR) in shake flasks, revealing that OTR could be used as a proxy for oxygen uptake rate and providing deeper insights into the cultivation process. Comparison between glass and polycarbonate flasks showed similar mass transfer characteristics and culture behavior. Monitoring of OTR proved useful for comparing different media and significantly increased the information content and data amount from shake flask experiments.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Robert Dinger, Clemens Lattermann, David Flitsch, Jan P. Fischer, Udo Kosfeld, Jochen Buechs
Summary: Mini-bioreactors with integrated online monitoring capabilities are widely used in the early stages of process development. However, the current OTR monitoring on a small scale is limited. In this study, a new micro-scale Transfer rate Online Measurement device (mu TOM) was developed and used for OTR monitoring. The mu TOM significantly increased throughput and decreased media consumption.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Energy & Fuels
Aicha Asma Houfani, Nico Anders, Judith Loogen, Benedikt Heyman, Zahra Azzouz, Azzeddine Bettache, Jochen Buchs, Said Benallaoua
Summary: The bacterium strain Bosea sp. FBZP-16 was found to produce various cellulases and hemicellulases under different growth conditions, with the highest activities observed on different substrates. Optimal production was achieved at temperatures between 30-35°C, shaking frequencies of 150-300 rpm, and the highest yields of glucose and xylose at 150 rpm. The potential of the strain to grow on xylan was confirmed, and using response surface methodology, optimal conditions for cellobiohydrolase and endoxylanase production were determined.
BIOMASS CONVERSION AND BIOREFINERY
(2021)
Article
Engineering, Environmental
Sarah Johann, Fabian G. Weichert, Lukas Schroeer, Lucas Stratemann, Christoph Kaempfer, Thomas-Benjamin Seiler, Sebastian Heger, Alexander Toepel, Tim Sassmann, Andrij Pich, Felix Jakob, Ulrich Schwaneberg, Peter Stoffels, Magnus Philipp, Marius Terfruechte, Anita Loeschcke, Kerstin Schipper, Michael Feldbruegge, Nina Ihling, Jochen Buechs, Isabel Bator, Till Tiso, Lars M. Blank, Martina Ross-Nickoll, Henner Hollert
Summary: This article introduces the interdisciplinary proof-of-concept project GreenToxiConomy, which successfully applies a Green Toxicology strategy to biosurfactants and a novel microgel-based pesticide release system. By using a bioassay battery, the acute and mechanism-specific toxicity of these potentially hazardous materials are evaluated in aquatic and terrestrial model species, and in silico models are used to predict toxicity. The most promising candidates are then selected for further experimental assessment.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Katharina Maria Saur, Robert Kiefel, Paul-Joachim Niehoff, Jordy Hofstede, Philipp Ernst, Johannes Brockkoetter, Jochem Gaetgens, Joern Viell, Stephan Noack, Nick Wierckx, Jochen Buechs, Andreas Jupke
Summary: This study demonstrates a holistic approach to biorefinery process development using sugar beet thick juice as a substrate for itaconic acid production with Ustilago sp. Fermentation can be successfully transferred from glucose to thick juice, and an additional decolorization step is necessary in downstream processing to remove impurities accompanying the crude substrate.
BIOENGINEERING-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Majharulislam Babor, Olivier Paquet-Durand, Christoph Berg, Jochen Buechs, Bernd Hitzmann
Summary: The use of 2D fluorescence spectra is a powerful method to estimate bioprocesses. This study substituted offline measurements with simulated values and utilized a mathematical process model to evaluate the process state. The results show that model-based calibration is an effective method for online estimation.
FERMENTATION-BASEL
(2023)
Meeting Abstract
Toxicology
E. C. Forsten, K. Kauffmann, A. Schiwy, H. Hollert, J. Buechs
TOXICOLOGY LETTERS
(2021)
Article
Biotechnology & Applied Microbiology
Marcel Mann, Darina Wittke, Jochen Buchs
Summary: Online monitoring of Clostridium ljungdahlii cultures with anaRAMOS device revealed iron(II) deficiency in YTF medium, and the addition of iron(II) led to shortened cultivation time and increased biomass yield. The study demonstrated the potential of anaRAMOS device for medium optimization in process development.
ENGINEERING IN LIFE SCIENCES
(2021)