Article
Biotechnology & Applied Microbiology
Holger Spiegel, Greta Noelke, Harry Thangaraj, Stefan Schillberg
Summary: Recombinant expression using Agrobacterium-mediated transient transformation (ATT) is a robust and versatile method for rapid protein production in plants. Plants have the ability to efficiently synthesize complex folded proteins with post-translational modifications, making them superior to microbial expression hosts. ATT requires lower skills and equipment compared to mammalian cell cultures, making it suitable for research and development purposes. However, the technology is not widely used outside the plant research community. The design of an ATT kit for educational and commercial purposes faces challenges related to regulatory and legal aspects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yuanchao Qian, Wentao Kong, Ting Lu
Summary: This study presents a synthetic biology toolkit for precise and effective control of gene expression in Agrobacterium tumefaciens. By constructing and characterizing inducible expression systems, optimizing regulation systems, and evaluating promoters, fine-tunability was established. The tools were successfully applied to co-express genes with altered expression levels, demonstrating their potential for advanced plant biotechnological applications.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Plant Sciences
Lauren Dickinson, Wenxin Yuan, Chantal LeBlanc, Geoffrey Thomson, Siyuan Wang, Yannick Jacob
Summary: During transformation via Agrobacterium tumefaciens, the sequence composition of T-DNA affects its concatenation in Arabidopsis, and the loss of specific DNA repair proteins dramatically decreases T-DNA concatenation levels. T-DNA concatenation can be used as a tool to enhance gene editing.
Article
Biotechnology & Applied Microbiology
Nisit Watthanasakphuban, Luan Van Nguyen, Yu-Shen Cheng, Pau-Loke Show, Malinee Sriariyanun, Mattheos Koffas, Kittipong Rattanaporn
Summary: The Agrobacterium-mediated transient gene expression system is a fast and efficient method for expressing recombinant proteins in plants. However, the high cost of Agrobacterium suspension production limits its application on an industrial scale. This study found that using sugarcane molasses as a low-cost carbon and nitrogen source can significantly reduce the production cost, without the need for antibiotic supplementation.
FERMENTATION-BASEL
(2023)
Article
Plant Sciences
Lauren Dickinson, Wenxin Yuan, Chantal LeBlanc, Geoffrey Thomson, Siyuan Wang, Yannick Jacob
Summary: The inclusion of retrotransposon sequences in T-DNA can significantly increase the copy number of T-DNA in Arabidopsis. This increase is primarily induced by the activity of retrotransposon sequences, and T-DNA concatenation depends on the activity of DNA repair proteins. This study demonstrates the potential of T-DNA concatenation as a tool to enhance gene editing.
Article
Biotechnology & Applied Microbiology
Isobel Dodds, Changlong Chen, Pierre Buscaill, Renier A. L. van Der Hoorn
Summary: Nicotiana benthamiana is increasingly used for transient gene expression but the expression is low in fully developed plants. The perception of cold shock protein (CSP) is thought to be the cause. This study demonstrates that silencing NbCORE in older plants increases GFP fluorescence and protein production, allowing for more efficient protein expression.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Jiali Zhang, Jing Zhang, Peiling Li, Yuan Gao, Qi Yu, Daojin Sun, Lingling Zhang, Siqi Wang, Jing Tian, Zhenxing Wang, Jiafu Jiang, Fadi Chen, Aiping Song
Summary: The diploid homozygous line Gojo-0 from a self-compatible mutant of Chrysanthemum seticuspe has been identified as a potential model plant for the genus Chrysanthemum. The study established optimal concentrations for growth regulators in regeneration and genetic transformation systems, and successfully verified transgenic plants through PCR detection, GUS staining, and high-throughput sequencing. This research provides a foundation for gene functional research on chrysanthemum and the identification of transgenic plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Hanqiang Liu, Jianyu Zhao, Feifan Chen, Zhiming Wu, Junyi Tan, Nhien Hao Nguyen, Zhihui Cheng, Yiqun Weng
Summary: In this study, the effects of various factors on shoot-regeneration rate and transformation efficiency in cucumber were evaluated. Factors such as genotypes, age and sources of explants, Agrobacterium strains, infection/co-cultivation conditions, and selective agents were examined. The results showed that North China cucumbers had higher shoot-regeneration rates compared to US pickling or slicing cucumbers. The use of Agrobacterium strain AGL1 and the antibiotic kanamycin were found to yield higher positive transformants and effective selection, respectively. The optimized protocol was successfully applied to develop transgenic plants and identify inheritable mutants. The overall transformation efficiency ranged from 0.2-1.7%. The Agrobacterium-mediated transformation protocol established in this study can serve as a starting point for further improvements in cucumber transformation.
Article
Immunology
Lesly Calderon, Karina Schindler, Stephen G. Malin, Alexandra Schebesta, Qiong Sun, Tanja Schwickert, Chiara Alberti, Maria Fischer, Markus Jaritz, Hiromi Tagoh, Anja Ebert, Martina Minnich, Adrian Liston, Luisa Cochella, Meinrad Busslinger
Summary: Pax5 plays a crucial role in mature B cells by inhibiting PTEN protein expression, which promotes differentiation and survival of B cells, thereby enhancing humoral immunity.
SCIENCE IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Vahid Mehrizadeh, Ebrahim Dorani, Seyed Abolghasem Mohammadi, Behzad Ghareyazie
Summary: The successful expression of recombinant human interferon-gamma (hIFN-gamma) protein in soybean using Agrobacterium-mediated transformation was reported in this study, along with confirmation of its antiviral activity. This finding can serve as a foundation for future research in large scale production of hIFN-gamma and other recombinant proteins in soybean.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Agronomy
Maria Aragona, Lidia Campos-Soriano, Edoardo Piombo, Elena Romano, Blanca San Segundo, Davide Spadaro, Alessandro Infantino
Summary: The study focused on the early interaction between Fusarium fujikuroi and rice roots, revealing that resistant cultivars trigger a hypersensitive response at the infection site and the expression of Chitinase1 gene is greatly increased in the resistant cultivar.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Plant Sciences
Niels Tol, Robin Schendel, Alex Bos, Maartje Kregten, Sylvia Pater, Paul J. J. Hooykaas, Marcel Tijsterman
Summary: Agrobacterium tumefaciens-mediated transformation is the preferred tool for generating transgenic plants, but gene targeting (GT) events occur at low frequency and are often accompanied by random integration events. The functionality of polymerase theta (Pol theta) affects the biology of GT in plants, with its deficiency preventing T-DNA integration and ectopic GT events. Insights obtained from this study can be used for developing strategies to achieve high-quality GT events in crop plants.
Article
Biochemistry & Molecular Biology
Hangyu Ye, Xiaojun Li, Luyuan Li, Yinjun Zhang, Jianyong Zheng
Summary: In this study, the alpha-L-rhamnosidase from A. niger (Rha-N1, GenBank XP_001389086.1) was successfully homologously expressed in A. niger 3.350 using Agrobacterium tumefaciens-mediated transformation. The purified Rha-N1 exhibited optimal activity at 60 degrees C and pH 4.5. The enzyme activity was influenced by various metal ions and organic solvents.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Review
Microbiology
Julie Baltenneck, Sylvie Reverchon, Florence Hommais
Summary: Quorum sensing is a mechanism by which bacterial populations coordinate gene expression through chemical communication, commonly used by pathogens to control virulence factor and environmental adaptation gene expression. Several quorum sensing mechanisms have been identified in phytopathogenic bacteria, utilizing different signal molecules. Research focuses on major phytopathogenic bacteria and their quorum sensing systems.
Article
Horticulture
Weijun Wu, Masaki Ochiai, Takashi Nakatsuka, Kunio Yamada, Hirokazu Fukui
Summary: It was found that ipt expression analysis can be used to evaluate crown gall disease resistance in rose progenies, and eight progenies with strong crown gall disease resistance were confirmed in the study.
HORTICULTURE JOURNAL
(2021)
Article
Horticulture
Jee-Soo Park, Jae-Gyeong Yu, Young-Doo Park
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY
(2017)
Article
Horticulture
Jung-Woo Lee, Ick-Hyun Jo, Jang-Uk Kim, Chi-Eun Hong, Young-Chang Kim, Dong-Hwi Kim, Young-Doo Park
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY
(2018)
Article
Horticulture
Jee-Soo Park, Jae-Gyeong Yu, Gi-Ho Lee, Young-Doo Park
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
So Young Jeong, Hyomin Ahn, Jahee Ryu, Youngbin Oh, Ganeshan Sivanandhan, Kang-Hee Won, Young Doo Park, Jin-Soo Kim, Hyeran Kim, Yong Pyo Lim, Sang-Gyu Kim
PLANT BIOTECHNOLOGY REPORTS
(2019)
Article
Multidisciplinary Sciences
Jee-Soo Park, Ji-Hyun Park, Young-Doo Park
Article
Horticulture
Jung-Woo Lee, Ick-Hyun Jo, Jang-Uk Kim, Chi-Eun Hong, Kyong-Hwan Bang, Young-Doo Park
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY
(2019)
Article
Horticulture
Sol-ah Kim, YoungJi Jeon, Jee-Soo Park, Young-Doo Park
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY
(2019)
Article
Horticulture
Gi-Ho Lee, Na-Ri Shin, Young-Doo Park
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY
(2020)
Article
Horticulture
Gi-Ho Lee, Hyun-Min Lee, So-Jeong Kim, Young-Doo Park
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY
(2020)
Article
Horticulture
Gi-Ho Lee, Young-Doo Park
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY
(2020)
Article
Horticulture
Yun Hee Shin, Si Hyeock Lee, Young-Doo Park
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY
(2020)
Article
Horticulture
Ganeshan Sivanandhan, Sheng Bean Choi, Moon Jiae, Su Ryun Choi, Sang Gyu Kim, Young Doo Park, Yong Pyo Lim
HORTICULTURAL SCIENCE & TECHNOLOGY
(2019)
Article
Horticulture
Gi-Ho Lee, Gang-Seob Lee, Jae-Gyeong Yu, Yong-Hwan Kim, Young-Doo Park
HORTICULTURAL SCIENCE & TECHNOLOGY
(2018)
Article
Horticulture
Gi-Ho Lee, Gang-Seob Lee, Young-Doo Park
HORTICULTURAL SCIENCE & TECHNOLOGY
(2017)
Article
Horticulture
Jae-Gyeong Yu, Gi-Ho Lee, Young-Doo Park
KOREAN JOURNAL OF HORTICULTURAL SCIENCE & TECHNOLOGY
(2016)