Article
Biochemistry & Molecular Biology
Carolina Villarroya-Beltri, Ana Filipa B. Martins, Alejandro Garcia, Daniel Gimenez, Eduardo Zarzuela, Monica Novo, Cristina Del Alamo, Jose Gonzalez-Martinez, Gloria C. Bonel-Perez, Irene Diaz, Maria Guillamot, Massimo Chiesa, Ana Losada, Osvaldo Grana-Castro, Meritxell Rovira, Javier Munoz, Maria Salazar-Roa, Marcos Malumbres
Summary: Maintenance of stemness is regulated by CDC14, a CDK-counteracting phosphatase, which dephosphorylates UTF1 to trigger its degradation and control neural differentiation. Lack of CDC14 results in deficient neural system development and impaired neural differentiation from ESCs. CDC14 phosphatases are critical molecular switches linking cell cycle regulation and self-renewal.
Review
Biochemistry & Molecular Biology
Yong-Jin Kim, Amin Tamadon, Yoon-Young Kim, Byeong-Cheol Kang, Seung-Yup Ku
Summary: This review focuses on the current knowledge of epigenetic regulation of cardiomyocyte proliferation and differentiation from embryonic and induced pluripotent stem cells through histone modification and microRNAs, the maintenance of pluripotency, and its alteration during cardiac lineage differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Developmental Biology
Andrea L. Bredemeyer, Junedh M. Amrute, Andrew L. Koenig, Rachel A. Idol, Li He, Stephanie A. Luff, Carissa Dege, Jamison M. Leid, Joel D. Schilling, J. Travis Hinson, Mary C. Dinauer, Christopher M. Sturgeon, Kory J. Lavine
Summary: Tissue-resident macrophages play a crucial role in organ homeostasis, tissue repair, remodeling, and regeneration. Researchers have developed a system to generate macrophages that resemble tissue-resident or monocyte-derived subsets, allowing for the study of their function and potential applications in tissue engineering and regenerative medicine.
Review
Biochemistry & Molecular Biology
Yahong Wu, Weiwei Zhang
Summary: Pluripotent stem cells, including ESCs and iPSCs, are crucial for studying early development and treating diseases, with ubiquitination playing a key role in pluripotency regulation, particularly through the actions of E3 ligases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Shuang Zhao, Chuanyu Zhang, Jia Xu, Siying Liu, Lu Yu, Shang Chen, Hang Wen, Zongjin Li, Na Liu
Summary: This study identified potential interacting proteins with Dppa3 and found that Dppa3 safeguards the stability of Uhrf1 and Nanog by inhibiting proteasome-associated degradation in ES cells.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Julian O. Kimura, Marcela Bolanos, Lorenzo Ricci, Mansi Srivastava
Summary: This study reveals the cellular mechanism and molecular trajectory for the formation of adult pluripotent stem cells (aPSCs) during embryonic development. By utilizing lineage tracing and single-cell transcriptome profiling, the researchers identified a molecular trajectory for neoblast formation and showed that aPSCs derive from one lineage instead of multiple tissue-specific lineages.
Article
Chemistry, Multidisciplinary
Yongping Lin, Feng Zhang, Shaojie Chen, Xiyu Zhu, Jincheng Jiao, Yike Zhang, Zhaomin Li, Jiao Lin, Biao Ma, Minglong Chen, Peng-Yuan Wang, Chang Cui
Summary: Biophysical cues have been found to promote the cardiac differentiation of human pluripotent stem cells (hPSCs), but the underlying mechanism remains unclear. In this study, the use of a binary colloidal crystal substrate called 5PM was shown to influence the cell nucleus, cytoskeleton, and epigenetic states of hPSCs, leading to changes in chromatin accessibility and histone modifications. The effect was found to be dependent on cytoskeleton tension and the nuclear accumulation of SETDB1, a histone lysine methyltransferase. Knockdown of SETDB1 reversed the promotive effects of the 5PM on cardiac differentiation. Similar results were observed in human embryonic stem cells, suggesting that the biophysical impact of the 5PM surfaces on cardiac differentiation may be universal.
Article
Immunology
Quanyu Chen, Yu You, Yujun Zhang, Hongyu Zhang, Lianhua Bai
Summary: In this study, the transplantation of NG2(+) HSPs secreting HGF showed prolonged survival times and improved graft functions in a xenogeneic rat acute liver rejection model. The effects were likely associated with HGF secretion and could target recipient CD8(+) T cells by inducing their apoptosis.Interestingly, injection of cHGF also prolonged the survival times of recipient rats and increased the number of apoptotic xenoreactive CD8(+) T cells.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Oncology
Borja Sese, Sandra Iniguez-Munoz, Miquel Ensenyat-Mendez, Pere Llinas-Arias, Guillem Ramis, Javier I. J. Orozco, Silvia Fernandez de Mattos, Priam Villalonga, Diego M. Marzese
Summary: This study identifies a subpopulation of glioma stem cells (GSCs), known as core-GSCs (c-GSCs), in glioblastoma (GBM) patients that have a similar profile to embryonic stem cells (ESCs) and downregulated immune-associated pathways. The development of an induced c-GSC (ic-GSC) cellular model provides insights into the immune evasion mechanisms in GBM and potential targets for improving immunotherapy treatments.
Article
Cell & Tissue Engineering
Noviana Wulansari, Yanuar Alan Sulistio, Wahyu Handoko Wibowo Darsono, Chang-Hoon Kim, Sang-Hun Lee
Summary: The study reveals the previously unrecognized epigenetic regulatory role of JAK2 as an important mediator in maintaining undifferentiated mESCs, by modulating DNA and histone demethylation to establish an open epigenetic status for pluripotency genes.
Article
Biochemistry & Molecular Biology
Kaoru Tominaga, Eiji Sakashita, Katsumi Kasashima, Kenji Kuroiwa, Yasumitsu Nagao, Naoki Iwamori, Hitoshi Endo
Summary: Epigenetic factors play a crucial role in establishing functional organ systems during development, including brain development. However, the precise mechanism of epigenetic regulation in brain development and neurogenesis remains largely unknown. In this study, the researchers discovered the critical role of the Tip60 protein in brain development and the maintenance and function of neural stem/progenitor cells (NSCs). Tip60 deficiency led to multiple abnormalities in brain development, including microcephaly, reduced proliferation of developing brain cells, and impaired neural differentiation and migration. The study also revealed that Tip60 is involved in the transition from neurogenesis to gliogenesis during brain development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Neurosciences
Ranran Duan, Yang Gao, Ruya He, Lijun Jing, Yanfei Li, Zhe Gong, Yaobing Yao, Tingting Luan, Chaopeng Zhang, Li Li, Yanjie Jia
Summary: Ischemic stroke is a severe central nervous system disease with high disability and mortality rates. Current therapies like rt-PA and mechanical thrombectomy have limitations, prompting the exploration of new treatments like stem cell transplantation, particularly iPSCs, which show promise in overcoming challenges and offering neuroprotection in stroke treatment.
FRONTIERS IN NEUROSCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Isabelle Xavier Dias, Aline Cordeiro, Joao Antonio Matheus Guimaraes, Karina Ribeiro Silva
Summary: The burden of musculoskeletal disorders is increasing worldwide and the treatment options are limited. Induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSC) have emerged as an alternative therapeutic approach with a higher potential for treating musculoskeletal disorders.
Review
Cell Biology
Genie Min Ju Wu, Andy Chun Hang Chen, William Shu Biu Yeung, Yin Lau Lee
Summary: Mammalian female reproduction relies on the functionality of the ovary, which is determined by the quality of ovarian follicles. Recent studies have successfully generated ovarian follicles in-vitro from pluripotent stem cells. These cells hold the potential to form ovarian follicles or organoids when co-cultured with ovarian somatic cells. In addition, oocytes isolated from these organoids have been shown to be capable of fertilization in-vitro. While the efficiency of in-vitro folliculogenesis remains low, the establishment of pluripotent stem cell-based models provides valuable insights into folliculogenesis. This article reviews the developmental events during in-vivo follicular development and discusses the progress of generating primordial germ cell-like cells (PGCLCs), pre-granulosa cells, and theca cells in-vitro.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell & Tissue Engineering
Li-Tzu Wang, Ko-Jiunn Liu, Huey-Kang Sytwu, Men-Luh Yen, B. Linju Yen
Summary: Mesenchymal stem cell therapy for immune and inflammatory diseases is gaining popularity, with increasing evidence of safety in clinical trials. The shift from autologous to allogeneic stem cells, as well as the development of new products like cell-free exosomes, are notable trends in the field.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Seo-Young Lee, Jung-Hyun Park, Sangkyun Jeong, Bu-Yeo Kim, Yong-Kook Kang, Yang Xu, Sun-Ku Chung
Article
Oncology
Jinchul Kim, Lili Yu, Wancheng Chen, Yanxia Xu, Meng Wu, Dilyana Todorova, Qingshuang Tang, Bingbing Feng, Lei Jiang, Jingjin He, Guihua Chen, Xuemei Fu, Yang Xu
Article
Cell Biology
Youqin Xu, Kaiyuan Ji, Meng Wu, Bingtao Hao, Kai-tai Yao, Yang Xu
Article
Cell & Tissue Engineering
Xin Liu, Meiyan Wang, Tao Jiang, Jingjin He, Xuemei Fu, Yang Xu
Article
Cell Biology
Lili Yu, Kai-yuan Ji, Jian Zhang, Yanxia Xu, Yue Ying, Taoyi Mai, Shuxiang Xu, Qian-bing Zhang, Kai-tai Yao, Yang Xu
Review
Cell Biology
Xuemei Fu, Shouhai Wu, Bo Li, Yang Xu, Jingfeng Liu
Article
Multidisciplinary Sciences
Lili Yu, Jinchul Kim, Lei Jiang, Bingbing Feng, Yue Ying, Kai-yuan Ji, Qingshuang Tang, Wancheng Chen, Taoyi Mai, Wenlong Dou, Jianlong Zhou, Le-yang Xiang, Yang-fan He, Dinghua Yang, Qingjiao Li, Xuemei Fu, Yang Xu
NATURE COMMUNICATIONS
(2020)
Article
Cell Biology
Shuxiang Xu, Jinchul Kim, Qingshuang Tang, Qu Chen, Jingfeng Liu, Yang Xu, Xuemei Fu
Article
Biochemistry & Molecular Biology
Qingke Li, Qu Chen, Paul C. Klauser, Mengyuan Li, Feng Zheng, Nanxi Wang, Xiaoying Li, Qianbing Zhang, Xuemei Fu, Qian Wang, Yang Xu, Lei Wang
Article
Engineering, Environmental
Miaomiao Yuan, Shuxiang Xu, Qianbing Zhang, Bingxia Zhao, Bingbing Feng, Kaiyuan Ji, Lili Yu, Wancheng Chen, Meirong Hou, Yang Xu, Xuemei Fu
CHEMICAL ENGINEERING JOURNAL
(2020)
Article
Cell & Tissue Engineering
Jingfeng Liu, Tingcai Pan, Yan Chen, Ying Liu, Fan Yang, Qu Chen, Nasir Abbas, Mingyan Zhong, Qianbing Zhang, Yang Xu, Yin-xiong Li
STEM CELL RESEARCH
(2020)
Article
Cell & Tissue Engineering
Gaoyang Zhu, Yue Ying, Kaiyuan Ji, Xinyue Duan, Taoyi Mai, Jinchul Kim, Qingjiao Li, Lili Yu, Yang Xu
STEM CELL RESEARCH
(2020)
Article
Medicine, General & Internal
Dilyana Todorova, Yue Zhang, Qu Chen, Jingfeng Liu, Jingjin He, Xuemei Fu, Yang Xu
Article
Nanoscience & Nanotechnology
Miaomiao Yuan, Xiaoying Li, Jingfeng Liu, Youbin Zheng, Li Cheng, Ning Tang, Rongjun Zhang, Shuxiang Xu, Xuemei Fu, Hossam Haick, Yang Xu
Summary: The utilization of SESD for self-powered electrical stimulation in cell culture significantly enhances the efficiency of plasmid transfection, making it a promising platform for high-efficiency plasmid DNA transfection in biomedical research and drug delivery.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Review
Cell Biology
Lili Yu, Meng Wu, Gaoyang Zhu, Yang Xu
Summary: Metabolism plays a critical role in maintaining cellular homeostasis and metabolic abnormalities are considered as driving forces for cancer progression. The tumor suppressor p53 is involved in inducing cell cycle arrest, apoptosis, senescence, and ferroptosis, and also actively participates in the reprogramming of cellular metabolism. This review focuses on recent advances in understanding the interplay between p53 and the metabolism of glucose, fatty acid, and amino acid, and discusses how the deregulation of p53 in these processes could lead to cancer.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)