Review
Biochemistry & Molecular Biology
Yong-Jin Kim, Amin Tamadon, Yoon-Young Kim, Byeong-Cheol Kang, Seung-Yup Ku
Summary: This review focuses on the current knowledge of epigenetic regulation of cardiomyocyte proliferation and differentiation from embryonic and induced pluripotent stem cells through histone modification and microRNAs, the maintenance of pluripotency, and its alteration during cardiac lineage differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Yahong Wu, Weiwei Zhang
Summary: Pluripotent stem cells, including ESCs and iPSCs, are crucial for studying early development and treating diseases, with ubiquitination playing a key role in pluripotency regulation, particularly through the actions of E3 ligases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell & Tissue Engineering
Gamze Ayaz, Hualong Yan, Navdeep Malik, Jing Huang
Summary: The TP53 gene encodes the p53 protein, a tumor suppressor that plays a crucial role in maintaining genome stability. Recent studies have found that p53 is a critical regulator of pluripotency, self-renewal, differentiation, proliferation, and genome stability in embryonic stem cells.
Review
Cardiac & Cardiovascular Systems
Chrishan J. A. Ramachandra, Jasper Chua, Shuo Cong, Myu Mai Ja Kp, Winston Shim, Joseph C. Wu, Derek J. Hausenloy
Summary: Normal cardiac functions depend on continuous energy supply. Metabolic disturbances and impaired mitochondrial bioenergetics underlie various cardiac diseases, but specific treatments are lacking. Patient-derived iPSCs show promise for studying cardiomyopathies and novel therapies.
CARDIOVASCULAR RESEARCH
(2021)
Article
Cell & Tissue Engineering
Hiroki Ozawa, Azusa Kambe, Kodai Hibi, Satoshi Murakami, Akira Oikawa, Tetsuya Handa, Katsunori Fujiki, Ryuichiro Nakato, Katsuhiko Shirahige, Hiroshi Kimura, Nobuaki Shiraki, Shoen Kume
Summary: Human induced pluripotent stem cells (iPSCs) require high levels of methionine (Met) for maintaining pluripotency. Met deprivation leads to decreased levels of intracellular S-adenosyl-methionine (SAM), triggering differentiation and apoptosis of undifferentiated cells. Short-term Met deprivation affects the pluripotency network through epigenetic modifications, specifically on histone H3 trimethylation at lysine 4 (H3K4me3). The transcription start site (TSS) region of genes involved in transforming growth factor beta pathway, cholesterol biosynthetic process, as well as key pluripotent genes NANOG and POU5F1, are specifically affected by Met deprivation. Upon differentiation, loss of H3K27me3 occurs in many endodermal genes, leading to their activation and switching from a bivalent to a monovalent state (H3K4me3).
Review
Cell Biology
Muhammad Shahid Javaid, Tracie Tan, Naomi Dvir, Alison Anderson, Terence J. O'Brien, Patrick Kwan, Ana Antonic-Baker
Summary: This review reports the current information and progress in using induced pluripotent stem cells to generate patient-specific models of epilepsy, which can be used to study the pathological mechanisms of epilepsy and drug development.
Article
Developmental Biology
Andrea L. Bredemeyer, Junedh M. Amrute, Andrew L. Koenig, Rachel A. Idol, Li He, Stephanie A. Luff, Carissa Dege, Jamison M. Leid, Joel D. Schilling, J. Travis Hinson, Mary C. Dinauer, Christopher M. Sturgeon, Kory J. Lavine
Summary: Tissue-resident macrophages play a crucial role in organ homeostasis, tissue repair, remodeling, and regeneration. Researchers have developed a system to generate macrophages that resemble tissue-resident or monocyte-derived subsets, allowing for the study of their function and potential applications in tissue engineering and regenerative medicine.
Article
Multidisciplinary Sciences
Karlijn A. L. Hasaart, Freek Manders, Joske Ubels, Mark Verheul, Markus J. van Roosmalen, Niels M. Groenen, Rurika Oka, Ewart Kuijk, Susana M. Chuva de Sousa Lopes, Ruben van Boxtel
Summary: This study compares the mutation burden between cultured iPSCs and their isogenic embryonic cells, finding that iPSCs maintain genomic integrity during culture at a similar degree as pluripotent cells in vivo.
Article
Biochemistry & Molecular Biology
Ashley Coope, Zain Ghanameh, Olivia Kingston, Carl M. Sheridan, Richard Barrett-Jolley, Marie M. Phelan, Rachel A. Oldershaw
Summary: The integration of cell metabolism with signaling pathways, transcription factor networks and epigenetic mediators is crucial for coordinating molecular and cellular events during embryogenesis. The metabolic regulation of induced pluripotent stem cells (IPSCs) pluripotency is mediated by balancing glycolysis and oxidative phosphorylation. H-1 NMR metabolomics is an effective tool for monitoring metabolite changes during pluripotent cell differentiation, providing insights into optimizing media and environmental parameters for studying embryogenesis and translational applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Stefanie Fuchs, Ruben W. J. van Helden, Maury Wiendels, Mees N. S. de Graaf, Valeria V. Orlova, Christine L. Mummery, Berend J. van Meer, Torsten Mayr
Summary: Recent advances in microfluidic engineering have enabled the cultivation of human cells under more realistic physiological conditions. By integrating oxygen and pH sensors into microfluidic devices, cellular physiology can be controlled and monitored in real time. Using highly metabolic human-induced pluripotent stem cells (hiPSCs) as a model, monitoring the extracellular environment allowed for rapid optimization of the seeding protocol and assessment of cell metabolism with the same precision as conventional cell culture methods.
MATERIALS TODAY BIO
(2022)
Article
Cell & Tissue Engineering
Shuang Zhao, Chuanyu Zhang, Jia Xu, Siying Liu, Lu Yu, Shang Chen, Hang Wen, Zongjin Li, Na Liu
Summary: This study identified potential interacting proteins with Dppa3 and found that Dppa3 safeguards the stability of Uhrf1 and Nanog by inhibiting proteasome-associated degradation in ES cells.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Julian O. Kimura, Marcela Bolanos, Lorenzo Ricci, Mansi Srivastava
Summary: This study reveals the cellular mechanism and molecular trajectory for the formation of adult pluripotent stem cells (aPSCs) during embryonic development. By utilizing lineage tracing and single-cell transcriptome profiling, the researchers identified a molecular trajectory for neoblast formation and showed that aPSCs derive from one lineage instead of multiple tissue-specific lineages.
Article
Cell Biology
Ying Zhang, Jun Wei, Jiani Cao, Kehua Zhang, Yaojin Peng, Hongkui Deng, Jiuhong Kang, Guangjin Pan, Yong Zhang, Boqiang Fu, Shijun Hu, Jie Na, Yan Liu, Lei Wang, Lingmin Liang, Huanxin Zhu, Yu Zhang, Zi-Bing Jin, Jie Hao, Aijin Ma, Tongbiao Zhao, Junying Yu
Summary: "Requirements for Human-Induced Pluripotent Stem Cells" is the first set of guidelines in China on human-induced pluripotent stem cells. It provides detailed technical requirements and aims to promote international standardization.
CELL PROLIFERATION
(2022)
Review
Pharmacology & Pharmacy
Michelle Vanessa Kapchoup Kamga, Michael Reppel, Jurgen Hescheler, Filomain Nguemo
Summary: Long QT syndrome, Brugada syndrome, and catecholaminergic polymorphic ventricular tachycardia are genetic heart diseases caused by mutations in cardiac ion channels, leading to paroxysmal arrhythmias with different clinical manifestations and functional perturbations. Abnormal sodium and calcium signaling plays a crucial role in these diseases. Patient-specific in vitro models based on induced pluripotent stem cells can help address the underlying mechanisms and develop personalized therapies.
BIOCHEMICAL PHARMACOLOGY
(2021)
Review
Biology
Afshin Zare, Aria Salehpour, Arezoo Khoradmehr, Shabnam Bakhshalizadeh, Vahid Najafzadeh, Sahar Almasi-Turk, Mahdi Mahdipour, Reza Shirazi, Amin Tamadon
Summary: More research is being conducted on using stem cell lines to develop into cardiomyocytes for myocardial cell treatments. Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) have shown promise in treating various cardiac illnesses. This study explores the differentiation of these cells into cardiomyocytes from an epigenetic perspective, including histone acetylation, methylation, DNA alterations, RNA methylation, and mitochondrial mutations.
Article
Cell Biology
Shuxiang Xu, Jinchul Kim, Qingshuang Tang, Qu Chen, Jingfeng Liu, Yang Xu, Xuemei Fu
Article
Engineering, Environmental
Miaomiao Yuan, Shuxiang Xu, Qianbing Zhang, Bingxia Zhao, Bingbing Feng, Kaiyuan Ji, Lili Yu, Wancheng Chen, Meirong Hou, Yang Xu, Xuemei Fu
CHEMICAL ENGINEERING JOURNAL
(2020)
Article
Cell & Tissue Engineering
Jingfeng Liu, Tingcai Pan, Yan Chen, Ying Liu, Fan Yang, Qu Chen, Nasir Abbas, Mingyan Zhong, Qianbing Zhang, Yang Xu, Yin-xiong Li
STEM CELL RESEARCH
(2020)
Article
Cell & Tissue Engineering
Gaoyang Zhu, Yue Ying, Kaiyuan Ji, Xinyue Duan, Taoyi Mai, Jinchul Kim, Qingjiao Li, Lili Yu, Yang Xu
STEM CELL RESEARCH
(2020)
Article
Medicine, General & Internal
Dilyana Todorova, Yue Zhang, Qu Chen, Jingfeng Liu, Jingjin He, Xuemei Fu, Yang Xu
Article
Nanoscience & Nanotechnology
Miaomiao Yuan, Xiaoying Li, Jingfeng Liu, Youbin Zheng, Li Cheng, Ning Tang, Rongjun Zhang, Shuxiang Xu, Xuemei Fu, Hossam Haick, Yang Xu
Summary: The utilization of SESD for self-powered electrical stimulation in cell culture significantly enhances the efficiency of plasmid transfection, making it a promising platform for high-efficiency plasmid DNA transfection in biomedical research and drug delivery.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Plant Sciences
Jingfeng Liu, Xiaofei Huang, Dandan Liu, Kaiyuan Ji, Cheng Tao, Ren Zhang, Jian Chen
Summary: In this study, we demonstrated that DMB inhibits NSCLC progression by inducing cell cycle arrest and triggering cellular senescence through downregulating the c-Myc/HIF-1 alpha pathway. We also found that DMB suppresses cell migration by inhibiting epithelial-mesenchymal transition (EMT) and accelerates senescence by suppressing HIF-1 alpha expression in NSCLC cells. Additionally, DMB decreases c-Myc expression, an upstream regulator of HIF-1 alpha.
Review
Cell Biology
Lili Yu, Meng Wu, Gaoyang Zhu, Yang Xu
Summary: Metabolism plays a critical role in maintaining cellular homeostasis and metabolic abnormalities are considered as driving forces for cancer progression. The tumor suppressor p53 is involved in inducing cell cycle arrest, apoptosis, senescence, and ferroptosis, and also actively participates in the reprogramming of cellular metabolism. This review focuses on recent advances in understanding the interplay between p53 and the metabolism of glucose, fatty acid, and amino acid, and discusses how the deregulation of p53 in these processes could lead to cancer.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Gastroenterology & Hepatology
Jingfeng Liu, Zhiming Yuan, Qingwen Wang
Summary: Liver disease is a significant burden worldwide, and liver transplantation is the only option for end-stage or acute organ failure. However, the shortage of suitable donors and the need for aggressive immunosuppressive drugs limit its wide usage. Pluripotent stem cells derived hepatocytes provide a promising alternative with unlimited proliferative and differential potential. This review discusses the recent progress and future directions of using pluripotent stem cell-derived hepatocytes in treating acute liver failure and explores the opportunities and challenges in their clinical applications.
JOURNAL OF CLINICAL AND TRANSLATIONAL HEPATOLOGY
(2022)
Article
Cell Biology
Kaiyuan Ji, Wenlong Dou, Ningfang Zhang, Bolun Wen, Mingyan Zhong, Qianbing Zhang, Shuxiang Xu, Jianlong Zhou, Jingfeng Liu
Summary: This study reveals the important role of RARG in the tumorigenesis of pancreatic cancer and identifies it as a new therapeutic target for human pancreatic cancer.
CELL BIOLOGY INTERNATIONAL
(2023)
Article
Plant Sciences
Xian Lin, Jingfeng Liu, Yujiao Zou, Cheng Tao, Jian Chen
Summary: This study demonstrated that Xan can suppress the progression of non-small cell lung cancer by downregulating the PI3K-AKT pathway, inducing cell cycle arrest, promoting apoptosis, and inhibiting epithelial-mesenchymal transition (EMT). Additionally, a new model for evaluating the effectiveness of Xan as a drug in NSCLC treatment was proposed.
Article
Nanoscience & Nanotechnology
Jingfeng Liu, Xiaoying Shi, Rongjun Zhang, Miaomiao Zhang, Juan He, Jian Chen, Zheng Wang, Qingwen Wang
Summary: In this study, novel CoFe2O4-quantum dots with outstanding synergistic photothermal/photodynamic property were constructed to efficiently suppress non-small-cell lung cancer. The combination of CoFe2O4-QDs + NIR treatment induced apoptosis of NSCLC cells and promoted reactive oxygen species generation to trigger cell death through regulating PI3K/AKT pathway. Moreover, the CoFe2O4-QDs + NIR treatment successfully eliminated tumor xenografts in vivo without toxicity.
NANOSCALE RESEARCH LETTERS
(2021)