Review
Biotechnology & Applied Microbiology
Marcin Szustak, Edyta Gendaszewska-Darmach
Summary: Nanocellulose is a promising biocompatible biomaterial with good mechanical properties for use as a scaffold in cartilage tissue engineering. The limited regenerative capacity of cartilage due to its lack of vascularization and sparsely distributed chondrocytes poses challenges for cell infiltration, making nanocellulose scaffolds a potential solution. The scaffolds have been shown to induce stem cell differentiation into chondrocyte phenotypes, offering opportunities for cartilage tissue development.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Yan Zeng, Xiaokai Li, Xia Liu, Yuzhou Yang, Zhimin Zhou, Jincai Fan, Haiyue Jiang
Summary: This study introduces a novel cartilage tissue engineering scaffold, using PLLA porous microsphere-reinforced silk-based hybrid material, which achieves mechanical strength similar to natural cartilage by adjusting the amount of microspheres, with a porous structure conducive to chondrogenesis. Biological evaluations demonstrate that this scaffold shows great potential in promoting cartilage regeneration.
Article
Biochemistry & Molecular Biology
Simone Krueger, Alexander Riess, Anika Jonitz-Heincke, Alina Weizel, Anika Seyfarth, Hermann Seitz, Rainer Bader
Summary: The application of external electric fields can induce chondrogenic re-differentiation of chondrocytes in vitro, with effects being frequency-dependent. Further parameter optimizations are needed to improve the differentiation capacity of human cartilage cells in future studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Polymer Science
Liang Lu, Xifu Shang, Bin Liu, Weijian Chen, Yu Zhang, Shuyun Liu, Xiang Sui, Aiyuan Wang, Quanyi Guo
Summary: This paper explores the application value of tissue engineering in vitro for the construction of artificial cartilage. By seeding healthy porcine chondrocytes on articular cartilage extracellular matrix (ACECM) scaffolds, it was found that the cells performed well in terms of adhesion and growth on the scaffolds, and they secreted collagen type II. Moreover, the porcine ACECM scaffolds showed good biocompatibility and could be used for xenotransplantation without significant immune inflammatory response. Therefore, ACECM-oriented scaffold is an ideal natural biomaterial for cartilage tissue engineering.
Article
Biochemistry & Molecular Biology
Elke Gossla, Anne Bernhardt, Robert Tonndorf, Dilbar Aibibu, Chokri Cherif, Michael Gelinsky
Summary: Replacement of damaged cartilage tissue with tissue engineering faces challenges due to the limited self-healing capacity of the non-vascularized tissue. A new approach using chitosan flock scaffolds combined with alginate hydrogel has been developed to support chondrogenic differentiation of human chondrocytes, showing promising results in gene expression and extracellular matrix production for cartilage regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Thomas H. Ambrosi, Owen Marecic, Adrian McArdle, Rahul Sinha, Gunsagar S. Gulati, Xinming Tong, Yuting Wang, Holly M. Steininger, Malachia Y. Hoover, Lauren S. Koepke, Matthew P. Murphy, Jan Sokol, Eun Young Seo, Ruth Tevlin, Michael Lopez, Rachel E. Brewer, Shamik Mascharak, Laura Lu, Oyinkansola Ajanaku, Stephanie D. Conley, Jun Seita, Maurizio Morri, Norma F. Neff, Debashis Sahoo, Fan Yang, Irving L. Weissman, Michael T. Longaker, Charles K. F. Chan
Summary: Aging of skeletal stem cells in mice alters bone marrow signaling and differentiation of bone and blood lineages, resulting in fragile bones with poor regeneration. Aged SSCs have decreased bone-forming potential but produce more stromal lineages expressing pro-inflammatory cytokines, contributing to bone marrow niche transformation.
Article
Biochemistry & Molecular Biology
Ilona Uzieliene, Daiva Bironaite, Edvardas Bagdonas, Jolita Pachaleva, Arkadij Sobolev, Wei-Bor Tsai, Giedrius Kvederas, Eiva Bernotiene
Summary: This study investigated the chondrogenic responses of human bone marrow mesenchymal stem cells (BMMSCs) and osteoarthritis (OA) cartilage-derived chondrocytes in 3D hydrogels under mechanical load conditions. The results showed that chondrocytes had slower proliferation rate, smaller size, and higher intracellular calcium level compared to BMMSCs. Chondrocytes secreted more cartilage oligomeric matrix protein (COMP) and expressed collagen type II (COL2A1) and aggrecan (ACAN) genes but were more susceptible to mechanical load compared to BMMSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nutrition & Dietetics
Maria Guillan-Fresco, Eloi Franco-Trepat, Ana Alonso-Perez, Alberto Jorge-Mora, Veronica Lopez-Lopez, Andres Pazos-Perez, Maria Pineiro-Ramil, Rodolfo Gomez
Summary: Isoflavonoid compound found in beer, formononetin (FNT), has a negative impact on chondrocytes and increases the risk of osteoarthritis.
Article
Dentistry, Oral Surgery & Medicine
Janis R. Thamm, Youssef Jounaidi, Andreas Kolk, Vicki Rosen, Maria J. Troulis, Fernando P. S. Guastaldi
Summary: This study aimed to enhance tissue regeneration of fibrocartilage by using amine-(NH2)-functionalized biosilica-incorporated hydrogel scaffolds seeded with CD105 antigen-positive mesenchymal stem/progenitor cells (MSPCs). The results showed that biosilica particle incorporation improved the water uptake capacity and compression force of the scaffolds. Functionalization with amine-(NH2) groups significantly increased the differentiation potential of stem cells. Cell-seeded scaffolds demonstrated higher secretion of cartilage extracellular matrix proteins. This study suggests that preselecting CD105-positive phenotype in MSPCs enhances tissue regeneration, and biosilica nanoparticles may be a beneficial additive in tissue engineering of scaffolds.
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY
(2023)
Article
Multidisciplinary Sciences
Sarah Rubin, Ankit Agrawal, Johannes Stegmaier, Sharon Krief, Neta Felsenthal, Jonathan Svorai, Yoseph Addadi, Paul Villoutreix, Tomer Stern, Elazar Zelzer
Summary: The 3D MAPs pipeline allows for the characterization of morphogenetic behaviors of chondrocytes while retaining spatial context, aiding in the study of bone development. The analysis revealed a wide range of morphological changes, growth strategies, and cell organizations in bones, as well as abnormalities in Gdf5 KO tibias, providing insights into cellular mechanisms regulating the differentiation process.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Nadine Frerker, Tommy A. A. Karlsen, Maria Stensland, Tuula A. A. Nyman, Simon Rayner, Jan E. E. Brinchmann
Summary: Focal lesions of articular cartilage can cause pain and reduced joint function, leading to osteoarthritis if untreated. In vitro generated, scaffold-free autologous cartilage discs made from articular chondrocytes were compared to those made from bone marrow-derived mesenchymal stromal cells. Articular chondrocytes produced more extracellular matrix and contained more articular cartilage proteins, while mesenchymal stromal cells had more proteins associated with cartilage hypertrophy and bone formation. MicroRNA analysis suggested that differential expression of microRNAs played a key role in the differential synthesis of proteins. Therefore, articular chondrocytes are preferred for engineering articular cartilage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
R. Olmos-Juste, G. Larranaga-Jaurrieta, I. Larraza, S. Ramos-Diez, S. Camarero-Espinosa, N. Gabilondo, A. Eceiza
Summary: In this study, functional alginate and waterborne polyurethane scaffolds were developed for articular cartilage regeneration using 3D bioprinting technology. It was found that scaffolds with a higher alginate content showed better 3D printing performance, structural integrity, and cell viability. After 28 days of experiments, these scaffolds were capable of maintaining cell numbers and synthesizing glycosaminoglycans, indicating their potential application for in vitro regeneration of articular cartilage tissue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Developmental Biology
Azim Pothiawala, Berke E. Sahbazoglu, Bryan K. Ang, Nadine Matthias, Guangsheng Pei, Qing Yan, Brian R. Davis, Johnny Huard, Zhongming Zhao, Naoki Nakayama
Summary: It has been discovered that mesodermal progeny of human pluripotent stem cells can differentiate into two types of chondrogenic mesenchymal cells with distinct characteristics and functions. GDF5(+) cells can generate tissue similar to permanent cartilage, while SOX9(+) cells resemble neural crest-like progeny. These findings shed light on the cellular differentiation and molecular regulation mechanisms during cartilage development.
Article
Cell & Tissue Engineering
Anastasiia D. Kurenkova, Lei Li, Anna P. Usanova, Xiaogang Feng, Baoyi Zhou, Andrei A. Nedorubov, Alexey Lychagin, Andrei S. Chagin
Summary: Articular cartilage has limited ability to self-repair, and clinical approaches to cartilage regeneration are necessary. The current method involves expansion of autologous chondrocytes followed by reimplantation, but the outcome is often unsatisfactory due to dedifferentiation. In recent years, articular stem/progenitor cells (artSPCs) have been discovered, which have the potential to repair damaged cartilage. This study found that modulation of the Notch pathway during expansion facilitated chondrogenic potential of artSPCs and chondrocytes, while activation of the pathway abrogated this potential.
Article
Orthopedics
Christopher B. Erickson, Jake P. Newsom, Nathan A. Fletcher, Yangyi Yu, Francisco Rodriguez-Fontan, Shane A. Weatherford, Nancy Hadley-Miller, Melissa D. Krebs, Karin A. Payne
Summary: The study shows that quick release of alpha-VEGF in rats can reduce bony bar and vessel formation, while increasing cartilage repair tissue. This treatment does not affect limb lengthening and does not lead to detrimental side effects in the adjacent, uninjured area. This approach could have positive implications for children with physeal injuries.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Article
Multidisciplinary Sciences
Alexander R. A. Szojka, Colleen N. Moore, Yan Liang, Stephen H. J. Andrews, Melanie Kunze, Aillette Mulet-Sierra, Nadr M. Jomha, Adetola B. Adesida
Summary: Low oxygen and mechanical loading may regulate the fibrocartilaginous phenotype of the human inner meniscus, but their combined effect in engineered tissues is not well-studied. The study found that neither hypoxia under static culture nor hypoxia combined with dynamic compression significantly affected the expression of specific markers for the fibrocartilaginous matrix-forming phenotype in human meniscus fibrochondrocytes. Additionally, mechanical properties increased over a two-week loading period regardless of oxygen tension and loading regime.
Article
Orthopedics
Alexander R. A. Szojka, Yan Liang, Rita de Cassia Marqueti, Colleen N. Moore, Esra J. N. Erkut, Melanie Kunze, Aillette Mulet-Sierra, Nadr M. Jomha, Adetola B. Adesida
Summary: This study investigated the time course of fibrocartilage formation by human meniscus fibrochondrocytes under various static conditions. Mechanical properties of the fibrocartilage were positively correlated with glycosaminoglycan content and accumulation of hyaline cartilage-like matrix components, and were lower in hypoxia but increased continually in normoxia. The most mechanically competent tissues reached a fraction of the native meniscus instantaneous compression modulus and exhibited a hypertrophic matrix-forming phenotype, which was consistently suppressed under hypoxia.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Correction
Multidisciplinary Sciences
Alexander R. A. Szojka, Colleen N. Moore, Yan Liang, Stephen H. J. Andrews, Melanie Kunze, Aillette Mulet-Sierra, Nadr M. Jomha, Adetola B. Adesida
Article
Cell Biology
Pranidhi Baddam, Daniel Young, Garett Dunsmore, Chunpeng Nie, Farah Eaton, Shokrollah Elahi, Juan Jovel, Adetola B. Adesida, Antoine Dufour, Daniel Graf
Summary: This study uncovers the cellular and molecular abnormalities underlying NSD using gene expression, proteomics, and immunofluorescence analysis. The investigation provides a genetic example of an in vivo cartilage fate switch controlled by BMP2 and BMP7 balance. Similar cellular and molecular changes in NSD and knee OA suggest a related etiology underlying these cartilage abnormalities.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Samia Rahman, Alexander R. A. Szojka, Yan Liang, Melanie Kunze, Victoria Goncalves, Aillette Mulet-Sierra, Nadr M. Jomha, Adetola B. Adesida
Summary: Human IFP-MSC did not undergo chondrogenesis under 2% low oxygen tension, but chondrogenic medium with TGFβ3 induced in vitro chondrogenesis, which did not lead to endochondral ossification after implantation in athymic mice.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Margaret J. Vyhlidal, Adetola B. Adesida
Summary: MFCs play a crucial role in regulating the biomechanical properties of the knee meniscus, and understanding their mechanotransductive mechanism is essential for advancing research in this field.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Zhiyao Ma, David Xinzheyang Li, Melanie Kunze, Aillette Mulet-Sierra, Lindsey Westover, Adetola B. Adesida
Summary: This study evaluated the effects of mechanical loading and unloading on the OA-related profile changes of engineered meniscus tissues and explored the biological sex-related differences. The results showed that mechanical stimulation had different effects on gene expression and tissue properties depending on the loading or unloading conditions and the sex of the donors.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Cell Biology
Zhiyao Ma, Margaret J. Vyhlidal, David Xinzheyang Li, Adetola B. Adesida
Summary: This article reviews various mechanical stimuli applied in meniscus bioengineering over the past 10 years. By evaluating the effects of mechanical stimulation on bioengineered meniscus at both molecular and tissue levels, it provides an important foundation for meniscus tissue engineering and osteoarthritis modeling.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Review
Engineering, Biomedical
Xiaoyi Lan, Adetola Adesida, Yaman Boluk
Summary: This article discusses the impact of rheological properties of collagen hydrogels on micro-extrusion and layer-stacking stages of bioprinting. It emphasizes the importance of steady shear flow measurements and large amplitude oscillatory shear tests in evaluating the performance of collagen networks during bioprinting. The article also introduces the significance of non-Newtonian material functions such as first normal stress difference and extensional viscosity. The discussions on collagen networks can also apply to the bioprinting of other hydrogels.
BIOMEDICAL MATERIALS
(2022)
Article
Multidisciplinary Sciences
Zhiyao Ma, David Xinzheyang Li, Ryan K. W. Chee, Melanie Kunze, Aillette Mulet-Sierra, Mark Sommerfeldt, Lindsey Westover, Daniel Graf, Adetola B. Adesida
Summary: Osteoarthritis primarily affects mechanical load-bearing joints, with the knee being the most common. Knee osteoarthritis (KOA) is more prevalent, burdensome, and severe in females, and hormonal differences do not fully explain this. Mechanical unloading in spaceflight microgravity has been implicated in the development of arthritis, but the mechanisms and sex differences are unclear. In this study, engineered meniscus constructs were generated and compared between male and female donors under normal gravity and simulated microgravity conditions to investigate the molecular basis and sex differences in the development of KOA.
Article
Multidisciplinary Sciences
A. K. Aissiou, S. Jha, K. Dhunnoo, Z. Ma, D. X. Li, R. Ravin, M. Kunze, K. Wong, A. B. Adesida
Summary: Spaceflight or simulated spaceflight microgravity can induce osteoarthritic-like alterations in the articular and meniscal cartilages of rodents, but its effect on tissue-engineered cartilage developed from human cells is not well understood. This study investigates the response of tissue-engineered cartilage to simulated microgravity facilitated by parabolic flights and reveals that the transcriptome profile and secretome of the engineered cartilage tissues showed sex-dependent differences in their modulation. These findings provide a foundation for further exploration of microgravity's underlying mechanisms.
Article
Engineering, Biomedical
Xiaoyi Lan, Zhiyao Ma, Melanie Kunze, Aillette Mulet-Sierra, Martin Osswald, Khalid Ansari, Hadi Seikaly, Yaman Boluk, Adetola B. Adesida
Summary: Hydrogels with different crosslinking densities were used to investigate their effects on chondrocyte phenotype and cellular interaction. The results showed that the storage modulus of the hydrogel did not significantly alter the cartilaginous gene expression of chondrocytes. However, the gene expression of PPAR-gamma was positively correlated with crosslinking density. RNA-seq analysis revealed numerous genes that were significantly correlated to crosslinking density, which warrants further investigation.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Article
Engineering, Biomedical
Xiaoyi Lan, Yaman Boluk, Adetola B. Adesida
Summary: Due to the limited self-repair capacity of hyaline cartilage, tissue engineering strategy with 3D bioprinting technique has emerged as a new solution for hyaline cartilage repair and regeneration. This review summarizes the intrinsic property of hyaline cartilage and discusses the selection of bioprinters, bioink materials, and cell sources for successful bioprinting. The aim of this review is to identify current challenges and future directions for clinical development of bioprinted hyaline cartilage.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Article
Cell & Tissue Engineering
David Xinzheyang Li, Zhiyao Ma, Alexander R. A. Szojka, Xiaoyi Lan, Melanie Kunze, Aillette Mulet-Sierra, Lindsey Westover, Adetola B. Adesida
Summary: This study introduced a novel protocol to differentiate hBM-MSC into stable, cartilage-forming cells. By combining mechano-hypoxia conditioning with TGF-beta 3 growth factor withdrawal, non-hypertrophic chondrogenesis of hBM-MSC can be promoted.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Cell & Tissue Engineering
Xiaoyi Lan, Yan Liang, Margaret Vyhlidal, Esra J. N. Erkut, Melanie Kunze, Aillette Mulet-Sierra, Martin Osswald, Khalid Ansari, Hadi Seikaly, Yaman Boluk, Adetola B. Adesida
Summary: This study investigated the impact of culture period on the formation and mechanical properties of 3D bioprinted constructs of engineered nasal cartilage using human nasal chondrocytes (hNC)-laden collagen hydrogel. The results demonstrated that these 3D bioprinted constructs were comparable or better than the clinically approved controls in terms of ECM formation and mechanical properties, making them feasible options for nasal cartilage reconstructive surgeries.
JOURNAL OF TISSUE ENGINEERING
(2022)
