Review
Biochemistry & Molecular Biology
Dileep Sai Kumar Palur, Shannon R. Pressley, Shota Atsumi
Summary: Human milk oligosaccharides (HMOs) are complex sugars in human milk that have various beneficial properties. The limited availability of HMOs has led to efforts in developing industrial-scale production platforms using microbial systems, particularly Escherichia coli. This review summarizes the progress in microbial production of HMOs, focusing on five approved HMOs for commercialization.
Article
Biotechnology & Applied Microbiology
Angela Zhang, Lei Sun, Yuanyuan Bai, Hai Yu, John B. McArthur, Xi Chen, Shota Atsumi
Summary: The study established a strategy for producing LDFT from lactose and L-fucose in Escherichia coli, using two bacterial fucosyltransferases to drive sequential fucosylation of lactose and intermediate 2'-FL. By optimizing cellular pathways, enhancing substrate transporters expression and inducing genes in the biosynthetic pathway, complete conversion of lactose into LDFT was achieved, with promising applications of engineered microbial biosystems for the production of multi-fucosylated HMOs for biochemical studies.
METABOLIC ENGINEERING
(2021)
Article
Allergy
Caroline J. Lodge, Adrian J. Lowe, Elasma Milanzi, Gayan Bowatte, Michael J. Abramson, Helen Tsimiklis, Christine Axelrad, Bianca Robertson, Aaron E. Darling, Cecilie Svanes, Matthias Wjst, Shyamali C. Dharmage, Lars Bode
Summary: In this high-allergy-risk birth cohort, certain profiles of human milk oligosaccharides (HMOs) were found to be associated with increased risks of allergic diseases in childhood, while others were associated with decreased risks. Further studies are needed to confirm these findings and explore their potential for intervention.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Chemistry, Applied
Rui Li, Yalin Zhou, Yajun Xu
Summary: In this study, UPLC-QE-HF-MS was used to detect human milk oligosaccharides, bovine milk oligosaccharides, goat milk oligosaccharides, and rat milk oligosaccharides. The four milk oligosaccharides showed significant differences in number and composition. Rat milk oligosaccharides were found to contain 14 neutral and 3 acidic oligosaccharides for the first time. The composition and abundances of rat milk oligosaccharides were more similar to human milk oligosaccharides, suggesting their potential use as models in biological/biomedical studies. Bovine milk oligosaccharides and goat milk oligosaccharides could have applications in medical and functional foods as promising bioactive molecules.
Article
Nutrition & Dietetics
Yuting Fan, Anita Vinjamuri, Diane Tu, Carlito B. Lebrilla, Sharon M. Donovan
Summary: Human milk oligosaccharides (HMOS) are indigestible carbohydrates that support infant development. The composition of HMOS varies among mothers, and factors affecting HMOS composition are limited.
FRONTIERS IN NUTRITION
(2023)
Article
Chemistry, Applied
Chuncui Huang, Yue Lu, Lin Kong, Zhendong Guo, Keli Zhao, Zheng Xiang, Xinyue Ma, Huanyu Gao, Yongfang Liu, Zhongmin Gao, Lijuan Xu, Wengang Chai, Yan Li, Yao Zhao
Summary: Human milk oligosaccharides (HMOs) are important for protecting infants from infections, especially for preterm infants. However, there is limited research on the specific differences in HMOs as a function of gestation time. In this study, we established an approach to analyze HMOs and found that the composition and relative abundance of HMOs changed with postpartum age, with significant differences in HMOs secreted at different postpartum times.
CARBOHYDRATE POLYMERS
(2023)
Editorial Material
Biochemistry & Molecular Biology
Lisa F. Stinson, Donna T. Geddes
Summary: Microbial metabolites are transmitted to infants via human milk, impacting early-life programming. The use of metabolomics is advocated to complement current milk microbiome research.
TRENDS IN MICROBIOLOGY
(2022)
Review
Biochemical Research Methods
Wenting Zhou, Hong Jiang, Lili Wang, Xingxing Liang, Xiangzhao Mao
Summary: 2'-FL is a key fucosylated HMO with significant value in nutrition and medicine. Traditional methods for producing 2'-FL are unable to meet demand, leading to the development of biotechnological approaches that improve efficiency and sustainability.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Chemistry, Applied
Wenyuan Zhang, Jacques Vervoort, Jiancun Pan, Peng Gao, Huiquan Zhu, Xiaodan Wang, Yumeng Zhang, Baorong Chen, Ying Liu, Yuanyuan Li, Xiaoyang Pang, Shuwen Zhang, Shilong Jiang, Jing Lu, Jiaping Lyu
Summary: Human milk oligosaccharides (HMOs) play a vital role in the development of the infant's gut microbiome and immune function. This study measured 12 oligosaccharides in milk from Chinese donors and evaluated the impacts of various factors on the HMOs profiles. The results showed that the concentrations of 6 sialyllactose were the highest among the 12 oligosaccharides. HMOs concentrations varied depending on geographical location, environmental factors, maternal anthropometric factors, and dietary factors.
Article
Agriculture, Multidisciplinary
Fan Liu, Jingyu Yan, Xifan Wang, Chenyuan Wang, Lingli Chen, Yiran Li, Jianliang Chen, Huiyuan Guo
Summary: This study analyzed the profiles of human milk oligosaccharides (HMOs) from secretor or non-secretor mothers and the fecal microbiota composition of breastfed infants. The results showed higher levels of total and fucosylated HMOs in secretor mothers, while Bifidobacterium genus was more abundant in infants fed by non-secretor mothers. Associations between specific HMOs and Bifidobacterium strains were also found.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemical Research Methods
Jiameng Zhang, Yingying Zhu, Wenli Zhang, Wanmeng Mu
Summary: In this study, a plasmid-based pathway optimization along with chromosomal integration strategies was used to successfully synthesize 3'-Sialyllactose (3'-SL). By deleting specific genes and optimizing key enzyme expression, the production of 3'-SL was significantly improved. The high yield achieved in large-scale fermentation is of great importance for the production of 3'-SL.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Yuanlin Liu, Yingying Zhu, Hao Wang, Li Wan, Wenli Zhang, Wanmeng Mu
Summary: Human milk oligosaccharides, particularly 2'-fucosyllactose (2'-FL), have unique health effects for growing infants. Metabolic engineering strategies have been used to enhance the production of 2'-FL through enhancing GDP-L-fucose supply and introducing alpha 1,2-fucosyltransferase.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Food Science & Technology
Wusun Li, Jingxuan Wang, Yingying Lin, Yixuan Li, Fazheng Ren, Huiyuan Guo
Summary: Human milk oligosaccharides (HMOs) are structurally diverse sugars highly abundant in human milk, with beneficial functions on infants such as modulating gut microbiota and promoting brain development. While some HMOs have been demonstrated safe to be added into infant formula, their application is currently limited by insufficient availability, calling for further basic and clinical studies in this field.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2021)
Review
Food Science & Technology
Elif Sakarya, Nazli Tunca Sanlier, Nevin Sanlier
Summary: The intestinal microbiota begins to form in the mother's womb and undergoes changes influenced by various factors. It plays a crucial role in immune system maturation and the prevention of diseases throughout different stages of life. Nutrition is a key factor in the development of the microbiota in infants after birth, with breast milk oligosaccharides playing a vital role. Breastfed infants have a higher number of Bifidobacterium species and lower diversity, which is considered protective. Dysbiosis in the microbiota can have adverse effects on health, but human milk oligosaccharides and short-chain fatty acids resulting from their digestion have protective effects. Another component, HM glycoproteins, also shape the gut microbiota. The aim of this study is to examine the impact of breast milk on microbiota development and present the findings from a literature review.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Nutrition & Dietetics
Linda P. Siziba, Marko Mank, Bernd Stahl, John Gonsalves, Bernadet Blijenberg, Dietrich Rothenbacher, Jon Genuneit
Summary: The study found that HMO concentrations in breast milk generally decreased over lactation, with some exceptions. The trajectories of HMO concentrations were mainly attributed to milk group and secretor status. Further investigation is warranted to determine the potential impact of these variations on child health outcomes.
Article
Biochemistry & Molecular Biology
Enrique Vazquez, Alejandro Barranco, Maria Ramirez, Agnes Gruart, Jose M. Delgado-Garcia, Esther Martinez-Lara, Santos Blanco, Maria Jesus Martin, Esther Castanys, Rachael Buck, Pedro Prieto, Ricardo Rueda
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2015)
Review
Nutrition & Dietetics
Esther Castanys-Munoz, Maria J. Martin, Pedro Antonio Prieto
Article
Multidisciplinary Sciences
Karen C. Goehring, Adam D. Kennedy, Pedro A. Prieto, Rachael H. Buck
Article
Food Science & Technology
Marie E. Latulippe, Agnes Meheust, Livia Augustin, David Benton, Premysl Bercik, Anne Birkett, Alison L. Eldridge, Joel Faintuch, Christian Hoffmann, Julie Miller Jones, Cyril Kendall, Franco Lajolo, Gabriela Perdigon, Pedro Antonio Prieto, Robert A. Rastall, John L. Sievenpiper, Joanne Slavin, Elizabete Wenzel de Menezes
FOOD & NUTRITION RESEARCH
(2013)
Article
Chemistry, Analytical
Gregory L. Hostetler, Cynthia M. Barber, Michael B. Miklus, Pedro A. Prieto
Summary: An LC-MS method was developed and validated for quantitation of casein in hypoallergenic infant formula, with a linear range of 1.8-42 μg/g. The method demonstrated good repeatability and precision, with recovery of casein ranging from 78% to 118% from incurred material.
JOURNAL OF AOAC INTERNATIONAL
(2021)
Article
Nutrition & Dietetics
Rosa Maria Espinosa, Martha Tamez, Pedro Prieto
BRITISH JOURNAL OF NUTRITION
(2007)
Article
Gastroenterology & Hepatology
RM Erney, WT Malone, MB Skelding, AA Marcon, KM Kleman-Leyer, ML O'Ryan, G Ruiz-Palacios, MD Hilty, LK Pickering, PA Prieto
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
(2000)
Article
Microbiology
JL Leach, SA Garber, AA Marcon, PA Prieto
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2005)
Article
Medicine, Research & Experimental
B Kelder, R Erney, J Kopchick, R Cummings, P Prieto
BIOACTIVE COMPONENTS OF HUMAN MILK
(2001)
Article
Medicine, Research & Experimental
R Erney, M Hilty, L Pickering, G Ruiz-Palacios, P Prieto
BIOACTIVE COMPONENTS OF HUMAN MILK
(2001)
Review
Nutrition & Dietetics
Chong-Su Kim
Summary: The gut microbiota plays a crucial role in brain function through the gut-brain axis. This review highlights the effects of gut microbiota-derived dietary metabolites on cell-to-cell interactions in the central nervous system, particularly microglia, astrocytes, and neuronal cells, affecting cognitive function, mood, and behavior. The review also discusses the potential of diet-induced microbial metabolite-based therapies as novel approaches to mental health treatment.
ADVANCES IN NUTRITION
(2024)