Article
Cell Biology
Vanessa Schimek, Katharina Strasser, Andrea Beer, Samantha Goeber, Natalie Walterskirchen, Christine Brostjan, Catharina Mueller, Thomas Bachleitner-Hofmann, Michael Bergmann, Helmut Dolznig, Rudolf Oehler
Summary: Sporadic apoptosis of tumour cells in colorectal cancer is associated with poor patient prognosis. Apoptotic cancer cells release chemotactic factors that attract neutrophils, inducing changes in neutrophil phenotype and lifespan and promoting interaction with macrophages in the tumor microenvironment, potentially contributing to an immunologically unfavorable environment.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Ludovica Lospinoso Severini, Elena Loricchio, Shirin Navacci, Irene Basili, Romina Alfonsi, Flavia Bernardi, Marta Moretti, Marilisa Conenna, Antonino Cucinotta, Sonia Coni, Marialaura Petroni, Enrico De Smaele, Giuseppe Giannini, Marella Maroder, Gianluca Canettieri, Angela Mastronuzzi, Daniele Guardavaccaro, Olivier Ayrault, Paola Infante, Francesca Bufalieri, Lucia Di Marcotullio
Summary: The Sonic Hedgehog (SHH) pathway plays a crucial role in embryonic development and stemness. The high expression of SALL4, a stemness regulator, is correlated with worse overall survival in patients with SHH-MB. SALL4 interacts with CRL3(REN) and enhances GLI1 transcriptional activity through cooperation with HDAC1. Inhibition of SALL4 suppresses the growth of SHH-MB in mouse models and patient-derived xenograft models, making it a promising therapeutic target.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Multidisciplinary Sciences
Mellissa C. Alcantara, Kentaro Suzuki, Alvin R. Acebedo, Daiki Kajioka, Satoshi Hirohata, Tsuneyasu Kaisho, Yu Hatano, Kazuo Yamagata, Satoru Takahashi, Gen Yamada
Summary: The study demonstrates that androgen induces cell migration through the transcription factor MafB, which remodels the local extracellular matrix (ECM) and leads to increased cell contractility and focal adhesion assembly. Additionally, matrix metalloproteinase Mmp11, as a target gene of MafB under androgen signaling, plays a role in ECM remodeling.
Article
Immunology
Ming Zhao, Kaiqun Ren, Xiwen Xiong, Yue Xin, Yujie Zou, Jason C. Maynard, Angela Kim, Alexander P. Battist, Navya Koneripalli, Yusu Wang, Qianyue Chen, Ruyue Xin, Chenyan Yang, Rong Huang, Jiahui Yu, Zan Huang, Zengdi Zhang, Haiguang Wang, Daoyuan Wang, Yihui Xiao, Oscar C. Salgado, Nicholas N. Jarjour, Kristin A. Hogquist, Xavier S. Revelo, Alma L. Burlingame, Xiang Gao, Jakob von Moltke, Zhaoyu Lin, Hai-Bin Ruan
Summary: Epithelial O-GlcNAc protein modification is induced by helminth infection, promoting the transcription of key genes and secretion of cytokines involved in immune responses and mucosal barrier regulation.
Article
Microbiology
Noemie Pied, Coralie F. Daussy, Zoe Denis, Jessica Ragues, Muriel Faure, Richard Iggo, Mario P. Tschan, Benoit Roger, Fabienne Rayne, Harald Wodrich
Summary: Intracellular pathogens cause membrane damage and cells respond by activating autophagy. The mechanism of autophagy induction during viral infections is rarely studied. This study investigates the cellular response to membrane damage during adenoviral entry and demonstrates that TBK1 kinase plays a crucial role in promoting autophagy in response to membrane penetration by adenoviruses. TBK1 is rapidly activated and recruited to the penetration sites, and its recruitment depends on damage recognition via galectin 8. This work highlights the importance of TBK1 in the cellular response to membrane damage and the activation of autophagy.
Article
Oncology
Ning Jiang, Yihao Liao, Miaomiao Wang, Youzhi Wang, Keke Wang, Jianing Guo, Peikang Wu, Boqiang Zhong, Tao Guo, Changli Wu
Summary: The study revealed that increased expression of BUB1 in human bladder cancer was associated with the activation of STAT3 signaling. Inhibition of BUB1 kinase activity effectively suppressed BCa cell proliferation and cancer progression both in vitro and in vivo. The BUB1/STAT3 complex played a critical role in promoting the transcription of STAT3 target genes, highlighting the potential of BUB1 as a therapeutic target for bladder cancer treatment.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Multidisciplinary Sciences
Yung-Hsuan Wu, Shu-Ting Mo, I. -Ting Chen, Fu-Yi Hsieh, Shie-Liang Hsieh, Jinake Zhang, Ming-Zong Lai
Summary: Inhibition of Caspase-8 leads to inflammasome assembly and production of IL-10 and IL-18 in Toll-like receptor (TLR)-primed myeloid cells. Autophagy and cathepsin-B play important roles in the activation of inflammasomes when apoptosis and necroptosis are blocked and Caspase-8 is inhibited.
Article
Cell Biology
Isha Kapoor, Juraj Bodo, Brian T. Hill, Alexandru Almasan
Summary: Aberrant microRNA expression, particularly in the 14q32 miRNA cluster region, plays a role in acquired resistance to BTK inhibitors in CLL and DLBCL cells. Overexpression of miRNAs such as miR-494, miR-495, and miR-543 is associated with downregulation of PTEN and further activation of the PI3K/AKT/mTOR pathway in resistant cells, impacting apoptosis regulation. Targeting these miRNAs may have therapeutic value in overcoming acquired BTK resistance.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Guadalupe Garcia, Karina A. Mendez-Resendiz, Norma Oviedo, Janet Murbartian
Summary: PKC and PKA phosphorylation inhibit TREK-1 channels downstream of G(s) protein-coupled receptor activation in vitro. This study investigated the role of phosphorylation of TREK-1 in neuropathic pain, finding that PKC and PKA modulators can alter TREK-1 function to contribute to antiallodynia in neuropathic rats, potentially through the phosphorylation sites S300 and S333.
JOURNAL OF NEUROCHEMISTRY
(2021)
Editorial Material
Biochemistry & Molecular Biology
Paul R. Clarke
Summary: The mechanism identified by Huang et al. stabilizes the interaction of RCC1 with chromatin through the arginine methyltransferase PRMT6, promoting cell proliferation and tumor formation. Targeting this mechanism may improve the treatment of tumors such as glioblastoma.
Article
Multidisciplinary Sciences
John Devany, Daniel M. Sussman, Takaki Yamamoto, M. Lisa Manning, Margaret L. Gardel
Summary: Epithelial tissues have distinctive cellular architectures that can be controlled by changes in cell shape. In a model epithelial monolayer, cell shape evolves over time, leading to a final architecture characterized by arrested motion and regular cell shapes. The final cell shape is closely correlated with cell proliferation rate, and pharmacological perturbations can significantly alter tissue dynamics by changing cell shapes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Oncology
Yonggang Ren, Jing Yang, Zhiran Ding, Menghua Zheng, Lu Qiu, Aifa Tang, Dandan Huang
Summary: Nuclear factor erythroid 2-like 3 (NFE2L3) is a transcription factor that promotes hepatocellular carcinoma (HCC) cell proliferation by directly inducing the expression of proteasome and interferon-stimulated gene 15 (ISG15), resulting in proteasome-dependent degradation of ISGylated p53. This post-translational modification destabilizes p53 and facilitates HCC cell growth. The findings suggest that NFE2L3 may serve as a new target for the clinical treatment of HCC.
Article
Oncology
Tengyang Ni, Zewen Chu, Li Tao, Yang Zhao, Miao Zhu, Yuanyuan Luo, Masataka Sunagawa, Haibo Wang, Yanqing Liu
Summary: PTBP1 is aberrantly highly expressed in gastric cancer and plays a crucial role in tumorigenesis and maintenance of stem-cell characteristics through stabilizing c-Myc protein. This study provides insights into the oncogenic role of PTBP1 and its involvement in gastric cancer progression.
BRITISH JOURNAL OF CANCER
(2023)
Article
Biochemistry & Molecular Biology
Fred Armbrust, Kira Bickenbach, Tomas Koudelka, Andreas Tholey, Claus Pietrzik, Christoph Becker-Pauly
Summary: Meprin beta is a zinc-dependent metalloprotease with a unique cleavage specificity for acidic amino acids at the cleavage site. Phosphorylation of meprin beta affects its cell surface activity and substrate cleavage extent, leading to internalization and degradation. The involvement of PKC isoforms in meprin beta phosphorylation was also demonstrated in this study.
Article
Cell Biology
Xin Dong, Xiaoying Li, Yu Gan, Jie Ding, Baojun Wei, Li Zhou, Wei Cui, Wei Li
Summary: The E3 ligase TNF receptor-associated factor 4 (TRAF4) is increased in colorectal cancer (CRC), and its depletion impairs CRC cell phenotype and enhances radiation-induced cell death. Irradiation activates the JNKs/c-Jun signaling, contributing to radioresistance by activating the antiapoptotic protein Bcl-xL. TRAF4 is positively correlated with c-Jun and Bcl-xL, and targeting the TRAF4/Bcl-xL axis sensitizes CRC cells to radiotherapy.
CELL DEATH & DISEASE
(2023)
Article
Physiology
Ilaria Musante, Paolo Scudieri, Arianna Venturini, Daniela Guidone, Emanuela Caci, Stefano Castellani, Massimo Conese, Luis J. V. Galietta
EXPERIMENTAL PHYSIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Jane Fraser, Joanne Simpson, Rosa Fontana, Chieko Kishi-Itakura, Nicholas T. Ktistakis, Noor Gammoh
Article
Cell Biology
Paolo Scudieri, Ilaria Musante, Arianna Venturini, Daniela Guidone, Michele Genovese, Federico Cresta, Emanuela Caci, Alessandro Palleschi, Marco Poeta, Francesca Santamaria, Fabiana Ciciriello, Vincenzina Lucidi, Luis J. V. Galietta
Article
Biochemistry & Molecular Biology
Valeria Capurro, Valeria Tomati, Elvira Sondo, Mario Renda, Anna Borrelli, Cristina Pastorino, Daniela Guidone, Arianna Venturini, Alessandro Giraudo, Sine Mandrup Bertozzi, Ilaria Musante, Fabio Bertozzi, Tiziano Bandiera, Federico Zara, Luis J. V. Galietta, Nicoletta Pedemonte
Summary: The deletion of phenylalanine at position 508 (F508del) in the CFTR chloride channel is the most common mutation in cystic fibrosis (CF) patients, and it can be overcome with small molecules called correctors, with VX-445 showing promising efficacy. However, even with corrector combinations, the behavior of F508del-CFTR is not fully normalized, indicating the potential for further improvements in rescuing mutant CFTR.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Elena Montano, Alessandra Pollice, Valeria Lucci, Geppino Falco, Ornella Affinito, Girolama La Mantia, Maria Vivo, Tiziana Angrisano
Summary: Identification of molecular mechanisms controlling early cell fate decisions in mammals is crucial for potential therapies. Ink4a and Arf from the Cdkn2a locus mark an early pancreatic cellular state resembling Pancreatic Progenitor Cells (PPCs). Chromatin remodeling may play a role in regulating the CDKN2a locus during pancreatic lineage commitment.
Article
Biochemistry & Molecular Biology
Rosa Fontana, Daniela Guidone, Tiziana Angrisano, Viola Calabro, Alessandra Pollice, Girolama La Mantia, Maria Vivo
Summary: ARF phosphorylation may be a mechanism through which it promotes or counteracts autophagy, providing evidence of its dual role in cancer progression.
Article
Genetics & Heredity
Mariarita Brancaccio, Caterina Giachino, Assunta Maria Iazzetta, Antonio Cordone, Elena De Marino, Ornella Affinito, Maria Vivo, Viola Calabro, Alessandra Pollice, Tiziana Angrisano
Summary: By analyzing the miRNA expression profiles, we identified key miRNAs in preeclampsia and discovered some upregulated miRNAs, which could be used as new non-invasive biomarkers and therapeutic targets for personalized treatments.
Article
Genetics & Heredity
Valeria Lucci, Elena De Marino, Daniela Tagliaferri, Stefano Amente, Alessandra Pollice, Viola Calabro, Maria Vivo, Geppino Falco, Tiziana Angrisano
Summary: Embryonic stem cells (ESCs) exhibit pluripotency heterogeneity corresponding to specific metastates. It has been discovered that retinoic acid (RA) can induce a specific 2C-like metastate, and Prame targets the genes Cdk8 and Cdkn2d, which are involved in cancer dedifferentiation and pluripotency.
Article
Medicine, Research & Experimental
Daniela Guidone, Martina Buccirossi, Paolo Scudieri, Michele Genovese, Sergio Sarnataro, Rossella De Cegli, Federico Cresta, Vito Terlizzi, Gabrielle Planelles, Gilles Crambert, Isabelle Sermet, Luis J. V. Galietta
Summary: The activity of ion channels and transporters controls the chemical and physical properties of the fluid on the surface of airway epithelia. In cystic fibrosis (CF), loss of CFTR chloride channel function leads to airway surface dehydration, bacterial infection, and inflammation. IL-17A and TNF-alpha cytokines have been found to upregulate genes related to ion transport, antibacterial defense, and neutrophil recruitment. Treatment with these cytokines also upregulates specific channels and transporters in bronchial epithelia. Importantly, a beta-adrenergic agonist is able to switch the airway surface to a low-viscosity state in non-CF epithelia but not in CF epithelia.
Article
Pharmacology & Pharmacy
Michele Genovese, Martina Buccirossi, Daniela Guidone, Rossella De Cegli, Sergio Sarnataro, Diego di Bernardo, Luis J. V. Galietta
Summary: The pharmacological inhibitors of TMEM16A have indirect effects on intracellular Ca2+ handling, which may impact their use as research tools. The mechanism of action of these inhibitors involves altering intracellular Ca2+ levels rather than directly inhibiting TMEM16A.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Multidisciplinary Sciences
Blanda Di Luccia, Vittoria Acampora, Anella Saggese, Viola Calabro, Maria Vivo, Tiziana Angrisano, Loredana Baccigalupi, Ezio Ricca, Alessandra Pollice
Summary: The gut microbiota plays a positive role in maintaining intestinal homeostasis through various mechanisms. This study investigated the effects of molecules secreted by Lactobacillus gasseri SF1183 on HCT116 cells and found that these molecules can reversibly reduce cell proliferation and protect cells from apoptosis. Additionally, they enhance the barrier function between cells.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Simone Amistadi, Giulia Maule, Matteo Ciciani, Marjolein M. Ensinck, Liesbeth De Keersmaecker, Anabela S. Ramalho, Daniela Guidone, Martina Buccirossi, Luis J. V. Galietta, Marianne S. Carlon, Anna Cereseto
Summary: This study developed a base editing strategy to correct the 2789+5G>A mutation in the CFTR gene, resulting in restoration of CFTR function and reduced bystander and off-target activities.
Review
Biochemistry & Molecular Biology
Rosa Fontana, Michela Ranieri, Girolama La Mantia, Maria Vivo