Article
Pharmacology & Pharmacy
Kayla Johnson, Alexis Doucette, Alexis Edwards, Aleeya Verdi, Ryan McFarland, Shelby Hulke, Amanda Fowler, Val J. Watts, Amanda H. Klein
Summary: This study found that reducing the activity or expression of adenylyl cyclase 1 can attenuate morphine tolerance and hypersensitivity, as well as provide relief for inflammatory pain.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tanya A. Baldwin, Yong Li, Autumn N. Marsden, Susanne Rinne, Anibal Garza-Carbajal, Roland F. R. Schindler, Musi Zhang, Mia A. Garcia, Venugopal Reddy Venna, Niels Decher, Thomas Brand, Carmen W. Dessauer
Summary: The establishment of macromolecular complexes by scaffolding proteins is crucial for the local production of cAMP and subsequent cAMP signaling necessary for cardiac functions. In this study, the researchers discovered a novel AC scaffold protein called POPDC1, which regulates heart rate control through its binding to the TREK-1 potassium channel. Furthermore, the activity of AC9 is required for the formation of the TREK-1:AC9:POPDC1 complex and the reversal of POPDC1's enhancement of TREK-1 currents.
Article
Biochemistry & Molecular Biology
Stephen C. Thornquist, Maximilian J. Pitsch, Charlotte S. Auth, Michael A. Crickmore
Summary: Research has found that a male fly's decision to persist in mating is based on a biochemical computation rather than patterns of fast electrical activity. In this computation, each neuron in a recurrent network contains slightly different internal molecular estimates, which are contrasted with input from other neurons through Protein kinase A (PKA) activity to generate a synchronized burst of calcium influx called an eruption. This biochemical activity is the key currency directing behavior and motivational state, playing a role invisible to most large-scale recording techniques.
Article
Multidisciplinary Sciences
Zhan-Guo Gao, Ian M. Levitan, Asuka Inoue, Qiang Wei, Kenneth A. Jacobson
Summary: Protein kinase C (PKC) isoforms can enhance A(2B) adenosine receptor (AR)-mediated cAMP accumulation through activation by phorbol 12-myristate 13-acetate (PMA), but do not enhance beta(2)-adrenergic receptor-mediated cAMP accumulation. PKC activation can also induce cAMP accumulation by activating A(2B)AR with high or low E-max. These findings are important for understanding the functions of A(2B)AR and PKC.
Article
Multidisciplinary Sciences
Tomohito Ohshima, Zhang Jiajun, Takuki Fukamachi, Yuko Ohno, Hiroko Senoo, Yasuhiro Matsuo, Makoto Kawamukai
Summary: This study identified a dominant negative allele, sam3, of the rad24 gene in the fission yeast Schizosaccharomyces pombe. The sam3 mutation allows mating without starvation and is caused by a change in nucleotide and amino acid at position 959 and 185, respectively. The Rad24-E185K mutant protein interacts differently with various proteins, leading to the sam phenotype and UV non-sensitivity. In addition, glucose depletion induces haploid meiosis in rad24-E185K or Delta pka1 Delta rad24 double mutants.
Article
Biochemistry & Molecular Biology
Qiusheng Shi, Jinpeng Gui, Lianwen Sun, Yaxin Song, Jing Na, Jingyi Zhang, Yubo Fan, Lisha Zheng
Summary: This study investigated the role of Frizzled-9 (Fzd9) in osteoblast dysfunction induced by simulated microgravity (SMG). It found that SMG led to a decrease in osteogenic markers and Fzd9 expression in osteoblasts. However, overexpression of Fzd9 counteracted this decrease. The study also revealed that Fzd9 overexpression regulated phosphorylation of glycogen synthase kinase-3 beta (pGSK3 beta) and beta-catenin, as well as protein kinase B (Akt) and extracellular signal-regulated kinase (ERK) phosphorylation, and induced actin polymerization and nuclear translocation of Yes-associated protein (YAP).
Article
Cardiac & Cardiovascular Systems
Mauro Gori, Brian Claggett, Michele Senni, Amil M. Shah, Ilan Goldenberg, Valentina Kutyifa, Dorit Knappe, Ann-Catherine Pouleur, Scott D. Solomon
Summary: The study demonstrated the independent prognostic value of diastolic dysfunction (DD) in patients with HFrEF, showing superior predictive power for heart failure compared to BNP. Integrating DD into conventional risk markers and BNP improves prediction accuracy for HF/death.
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2021)
Article
Cell Biology
Alessandra Ghigo, Alessandra Murabito, Valentina Sala, Anna Rita Pisano, Serena Bertolini, Ambra Gianotti, Emanuela Caci, Alessio Montresor, Aiswarya Premchandar, Flora Pirozzi, Kai Ren, Angela Della Sala, Marco Mergiotti, Wito Richter, Eyleen de Poel, Michaela Matthey, Sara Caldrer, Rosa A. Cardone, Federica Civiletti, Andrea Costamagna, Nancy L. Quinney, Cosmin Butnarasu, Sonja Visentin, Maria Rosaria Ruggiero, Simona Baroni, Simonetta Geninatti Crich, Damien Ramel, Muriel Laffargue, Carlo G. Tocchetti, Renzo Levi, Marco Conti, Xiao-Yun Lu, Paola Melotti, Claudio Sorio, Virginia De Rose, Fabrizio Facchinetti, Vito Fanelli, Daniela Wenzel, Bernd K. Fleischmann, Marcus A. Mall, Jeffrey Beekman, Carlo Laudanna, Martina Gentzsch, Gergely L. Lukacs, Nicoletta Pedemonte, Emilio Hirsch
Summary: This study identifies PI3Kγ as a critical regulator of the cAMP signaling microdomain activated by β(2)-ARs. By using a PI3Kγ mimetic peptide (PI3Kγ MP) to displace the PKA pool anchored by PI3Kγ, cAMP can be safely increased in the lungs, leading to airway smooth muscle relaxation and reduced inflammation.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Cell Biology
Marek M. Drozdz, Ashley S. Doane, Rached Alkallas, Garrett Desman, Rohan Bareja, Michael Reilly, Jakyung Bang, Maftuna Yusupova, Jaewon You, Zuhal Eraslan, Jenny Z. Wang, Akanksha Verma, Kelsey Aguirre, Elsbeth Kane, Ian R. Watson, Olivier Elemento, Elena Piskounova, Taha Merghoub, Jonathan H. Zippin
Summary: Nuclear cAMP microdomains can activate a tumor-suppressive pathway in a broad range of cancers by inhibiting the key effector protein YAP of the Hippo pathway.
Article
Pediatrics
Nicolas L. Madsen, Jessica E. Haley, Ryan A. Moore, Philip R. Khoury, Elaine M. Urbina
Summary: The study found that increased arterial stiffness in youth is associated with diastolic dysfunction, independent of cardiovascular disease risk factors. This suggests that improving arterial stiffness during adolescence may help prevent the development of heart failure.
FRONTIERS IN PEDIATRICS
(2021)
Article
Cell Biology
David A. Skerrett-Byrne, Amanda L. Anderson, Elizabeth G. Bromfield, Ilana R. Bernstein, Jess E. Mulhall, John E. Schjenken, Matthew D. Dun, Sean J. Humphrey, Brett Nixon
Summary: This study used quantitative mass spectrometry-based proteomics to investigate the changes associated with the maturation of mouse spermatozoa. It identified thousands of proteins and revealed important protein alterations and signaling pathway activation during sperm maturation.
Article
Oncology
Amos Hung, Yen-Yun Wang, Kun-Tsung Lee, Hung-Hsing Chiang, Yuk-Kwan Chen, Je-Kang Du, Chun-Ming Chen, Michael Chen, Kwei-Jing Chen, Stephen Hu, Shyng-Shiou F. Yuan
Summary: Resistin has been associated with esophageal squamous cell carcinoma (ESCC) and its expression levels are significantly lower in ESCC patients, positively correlated with patient's body mass index. Resistin inhibits the migratory ability of ESCC cells without affecting cell proliferation.
Article
Developmental Biology
Momoe Ito, Masato Unou, Toshiya Higuchi, Shuhei So, Masahiko Ito, Keiichiro Yogo
Summary: SLC22A14 gene plays a critical role in male infertility in mice by regulating HCO3- transport and intracellular pH during sperm capacitation.
Review
Peripheral Vascular Disease
Amy S. Shah, Sakthivel Sadayappan, Elaine M. Urbina
Summary: Obesity and type 2 diabetes in youth are associated with the development of heart failure with preserved ejection fraction (HFpEF) due to diabetes-related cardiomyopathy. The mechanisms include abnormal diastolic function and there are currently no effective therapies available for treating HFpEF.
CURRENT ATHEROSCLEROSIS REPORTS
(2022)
Article
Medicine, General & Internal
Rupa Lakshmi Iyengar-Kapuganti, Cynara S. Maceda, Lori B. Croft, Simonette T. Sawit, Laura E. Crowley, Mark Woodward, Mary Ann McLaughlin
Summary: This study investigated the relationship between obstructive sleep apnoea (OSA) and left ventricular diastolic dysfunction (LVDD) in first responders to the 9/11 World Trade Center (WTC) terrorist attack. The results showed a significant association between patient-reported OSA and LVDD, but the significance was not maintained after adjusting for pertinent variables. However, a significant relationship between OSA diagnosis and LVDD remained even after adjusting for other factors.
Article
Biochemistry & Molecular Biology
Tarsis F. Brust, Doungkamol Alongkronrusmee, Monica Soto-Velasquez, Tanya A. Baldwin, Zhishi Ye, Mingji Dai, Carmen W. Dessauer, Richard M. van Rijn, Val J. Watts
Article
Biochemistry & Molecular Biology
Tarsis F. Brust, Doungkamol Alongkronrusmee, Monica Soto-Velasquez, Tanya A. Baldwin, Zhishi Ye, Mingji Dai, Carmen W. Dessauer, Richard M. van Rijn, Val J. Watts
Article
Clinical Neurology
Hanneke L. D. M. Willemen, Niels Eijkelkamp, Anibal Garza Carbajal, Huijing Wang, Matthias Mack, Jitske Zijlstra, Cobi J. Heijnen, Annemieke Kavelaars
Article
Multidisciplinary Sciences
Yan Li, Xiaoyun Tan, Mengru Wang, Bingxuan Li, Yanxue Zhao, Chengyun Wu, Qingchen Rui, Junxia Wang, Zhongyuan Liu, Yiqun Bao
SCIENTIFIC REPORTS
(2017)
Article
Pharmacology & Pharmacy
Tanya A. Baldwin, Yong Li, Cameron S. Brand, Val J. Watts, Carmen W. Dessauer
MOLECULAR PHARMACOLOGY
(2019)
Article
Cell Biology
Yong Li, Thomas Hof, Tanya A. Baldwin, Lei Chen, Robert S. Kass, Carmen W. Dessauer
Article
Neurosciences
Anibal Garza Carbajal, Alexis Bavencoffe, Edgar T. Walters, Carmen W. Dessauer
JOURNAL OF NEUROSCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Anibal Garza Carbajal, Andrea Ebersberger, Alina Thiel, Luiz Ferrari, Jeremy Acuna, Stephanie Brosig, Joerg Isensee, Katharina Moeller, Maike Siobal, Stefan Rose-John, Jon Levine, Hans-Georg Schaible, Tim Hucho
Summary: Hyperalgesic priming is characterized by enhanced nociceptor sensitization by pronociceptive mediators, with PGE(2) as a prototypical example. In this study, the cytokine OncostatinM was identified as an inducer of priming through a novel mechanism involving cAMP/PKA to ERK signaling. This finding sheds light on the complex cellular mechanisms underlying the transition to chronic pain.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Neurosciences
Elia R. Lopez, Anibal Garza Carbajal, Jin Bin Tian, Alexis Bavencoffe, Michael X. Zhu, Carmen W. Dessauer, Edgar T. Walters
Summary: Recent research has shown that serotonin promotes ongoing activity in nociceptors through activation of the 5-HT4 receptor and downstream cAMP signaling. This finding provides new insights and potential therapeutic targets for the study of neuropathic pain.
Article
Biochemistry & Molecular Biology
Tanya A. Baldwin, Yong Li, Autumn N. Marsden, Susanne Rinne, Anibal Garza-Carbajal, Roland F. R. Schindler, Musi Zhang, Mia A. Garcia, Venugopal Reddy Venna, Niels Decher, Thomas Brand, Carmen W. Dessauer
Summary: The establishment of macromolecular complexes by scaffolding proteins is crucial for the local production of cAMP and subsequent cAMP signaling necessary for cardiac functions. In this study, the researchers discovered a novel AC scaffold protein called POPDC1, which regulates heart rate control through its binding to the TREK-1 potassium channel. Furthermore, the activity of AC9 is required for the formation of the TREK-1:AC9:POPDC1 complex and the reversal of POPDC1's enhancement of TREK-1 currents.
Review
Cardiac & Cardiovascular Systems
Tanya A. Baldwin, Carmen W. Dessauer
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2018)