Article
Immunology
Noemi Alejandra Saavedra-Avila, Paolo Dellabona, Giulia Casorati, Natacha Veerapen, Gurdyal S. Besra, Amy R. Howell, Steven A. Porcelli
Summary: A genetically modified mouse model allows for more accurate prediction of human iNKT cell responses, providing opportunities for preclinical evaluation of iNKT cell-based therapies.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Minjoong Kim, Sunsook Hwang, Byungjoo Kim, Seungmin Shin, Seungyeon Yang, Jihye Gwak, Seung Min Jeong
Summary: Cells rely on glutamine metabolism for energy and cellular homeostasis. Activation of the YAP pathway in response to glutamine shortage induces the expression of ATF4 and Sestrin2, which are crucial for cell survival. Targeting the Hippo-YAP pathway in combination with glutaminolysis inhibition shows potential for tumor treatment.
Review
Neurosciences
Yundi Wang, Jeffrey M. LeDue, Timothy H. Murphy
Summary: Multiscale neurophysiology studies have shown that simple motor actions lead to changes in neuronal firing in almost every brain region. To assess focal pathologies such as stroke or neurodegenerative diseases, it is important to consider multiple resolution scales and multimodal factors, including specific neurons and glial cells, and the dynamics of neurotransmitters. Emerging multiscale methods in animal studies provide a circuit-based understanding of pathophysiological mechanisms, and when combined with computational tools and open-source data, they enable the discovery of new knowledge in stroke, Alzheimer's disease, and movement disorders.
Review
Immunology
Min Hu, Wayne J. Hawthorne, Shounan Yi, Philip J. O'Connell
Summary: Porcine islet xenografts face rejection primarily mediated by CD4(+) T cells, resulting in infiltration of macrophages, B cells, and T cells (CD4(+) and CD8(+)). Compared to alloimmune response, xenograft rejection is more difficult to suppress and involves the activation of CD4(+) T cells, recruitment of macrophages, and B cell responses. Macrophages are believed to be a major effector cell, while B cells play a crucial role in priming effector T cells and producing xenospecific antibodies. Strategies to expand regulatory T cell influence and inhibit and/or reduce macrophage and B cell responses are required for effective suppression of the T cell-initiated xenograft response in islet xenotransplantation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Seung-Hye Lee, Mitchell G. Rezzonico, Brad A. Friedman, Melanie H. Huntley, William J. Meilandt, Shristi Pandey, Ying-Jiun J. Chen, Amy Easton, Zora Modrusan, David Hansen, Morgan Sheng, Christopher J. Bohlen
Summary: In neurodegenerative diseases, non-neuronal responses have been recognized as important players in disease progression. Through single-cell RNA sequencing in mouse models of Alzheimer's disease, distinct transcriptional states were identified in oligodendrocytes and the impact of Trem2 deletion on microglial responses was explored, highlighting the complex interplay between different cell types in Alzheimer's disease pathology.
Review
Pharmacology & Pharmacy
Iqra Ilyas, Peter J. Little, Zhiping Liu, Yanyong Xu, Danielle Kamato, Bradford C. Berk, Jianping Weng, Suowen Xu
Summary: This article reviews the current use and translational potential of mouse models in atherosclerosis research and drug discovery. Atherosclerotic cardiovascular disease is a chronic and progressive metabolic and inflammatory disease that causes premature human mortality. Despite promising preclinical studies, many drug candidates have not been translated to clinical use due to the complex disease mechanisms involved.
TRENDS IN PHARMACOLOGICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Charissa Poon, Petteri Teikari, Muhammad Febrian Rachmadi, Henrik Skibbe, Kullervo Hynynen
Summary: In this study, the first annotated dataset of rodent cerebrovasculature, MiniVess, was introduced. The dataset consists of 70 3D image volumes with segmented ground truths, created using various methods. The availability of annotated datasets is crucial for the development and testing of machine learning tools for biomedical image analysis.
Article
Chemistry, Multidisciplinary
Jeff W. M. Bulte, Chao Wang, Ali Shakeri-Zadeh
Summary: Superparamagnetic iron oxide (SPIO)-labeling of cells for magnetic resonance imaging (MRI) cell tracking has a long history, but studies have shown that the labeled cells may have different behaviors compared to unlabeled cells. With the emergence of magnetic particle imaging (MPI) cell tracking, there has been a renaissance in the formulation of SPIO nanoparticles optimized for MPI. Lessons learned from past experiences with SPIO-labeling for MRI could potentially facilitate the clinical translation of (combined) MRI/MPI cell tracking.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Neurosciences
Mengfei Xiong, Sahar Roshanbin, Johanna Rokka, Eva Schlein, Martin Ingelsson, Dag Sehlin, Jonas Eriksson, Stina Syvanen
Summary: The study investigated synaptic density in transgenic mouse models of Alzheimer's disease and Parkinson's disease using [C-11]UCB-J PET, suggesting that the PET imaging reflects synaptic density in mouse models of neurodegeneration with large inter-subject variation. Additionally, the study proposed that the model-independent AUCbrain/blood ratio can be used to evaluate [C-11]UCB-J binding as an alternative to full pharmacokinetic modeling.
Article
Nanoscience & Nanotechnology
Marine Z. C. Hatit, Melissa P. Lokugamage, Curtis N. Dobrowolski, Kalina Paunovska, Huanzhen Ni, Kun Zhao, Daryll Vanover, Jared Beyersdorf, Hannah E. Peck, David Loughreyl, Manaka Sato, Ana Cristianl, Philip J. Santangelo, James E. Dahlman
Summary: This study compared the delivery of mRNA by lipid nanoparticles in different species using DNA barcoding technology and identified species-dependent responses. These findings contribute to the acceleration of RNA therapy development.
NATURE NANOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Seyoung Koo, Miae Won, Hao Li, Won Young Kim, Mingle Li, Chenxu Yan, Amit Sharma, Zhiqian Guo, Wei-Hong Zhu, Jonathan L. Sessler, Jin Yong Lee, Jong Seung Kim
Summary: The study showed that QM-NH alpha fuc proved effective in detecting various types of cellular senescence, including replicative, ROS, UVA, and drug-induced senescence, as well as identifying senescent cells lacking beta-gal expression. Additionally, the non-invasive real-time senescence tracking provided by QM-NH alpha fuc was validated in an in vivo senescence model. The results suggest that QM-NH alpha fuc could emerge as a useful tool for investigating senescence processes in biological systems.
Article
Biochemistry & Molecular Biology
Ulrich Gergs, Timo Gerigk, Jonas Wittschier, Constanze T. Schmidbaur, Clara Rottger, Mareen Mahnkopf, Hanna Edler, Hartmut Wache, Joachim Neumann
Summary: The study found that cardiac function is affected by 5-HT4 receptor overexpression under pathophysiological conditions, with potential benefits or drawbacks depending on the type of stress. 5-HT4 receptor overexpression influenced the response of the heart to hypoxia and ischemia, as well as cardiac hypertrophy.
Article
Multidisciplinary Sciences
Bryan E. Fowler, Lindsey J. Macpherson
Summary: Advances in genetically encoded calcium indicators (GECIs) have revolutionized in vivo functional imaging by monitoring the responses of individual cells to various stimuli in real time. Imaging the taste-evoked responses of geniculate ganglion neurons with GCaMP has provided important information about their tuning profiles and detecting taste miswiring phenotypes. This technique is a powerful tool for monitoring the activity of peripheral gustatory neurons and complements traditional methods like whole-nerve chorda tympani recordings or taste behavior assays.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Biochemistry & Molecular Biology
Shijie Guo, Weiwei Huang, Qingqing Cao, Qingbin Guo, Jianzhong Han, Yumei Qin
Summary: This study used intestinal organoids as an in vitro model to analyze the effects of four (epi)catechins on the development of the intestinal epithelial structure. The results showed that (epi)catechins promoted intestinal epithelial apoptosis and stress response, leading to intestinal damage and increased risk of intestinal injury.
Review
Immunology
Yanqin Du, Ruth Broering, Xiaoran Li, Xiaoyong Zhang, Jia Liu, Dongliang Yang, Mengji Lu
Summary: This review provides a comprehensive overview of the characteristics and limitations of various mouse models used in HBV research, aiming to guide future studies in this field.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Marc Clement, Gemma Basatemur, Leanne Masters, Lauren Baker, Patrick Bruneval, Takao Iwawaki, Manfred Kneilling, Sho Yamasaki, Jane Goodall, Ziad Mallat
Article
Immunology
Markus Tschurtschenthaler, Timon E. Adolph, Jonathan W. Ashcroft, Lukas Niederreiter, Richa Bharti, Svetlana Saveljeva, Joya Bhattacharyya, Magdalena B. Flak, David Q. Shih, Gwenny M. Fuhler, Miles Parkes, Kenji Kohno, Takao Iwawaki, C. Janneke van der Woude, Heather P. Harding, Andrew M. Smith, Maikel P. Peppelenbosch, Stephan R. Targan, David Ron, Philip Rosenstiel, Richard S. Blumberg, Arthur Kaser
JOURNAL OF EXPERIMENTAL MEDICINE
(2017)
Article
Cell Biology
Simon J. Tavernier, Fabiola Osorio, Lana Vandersarren, Jessica Vetters, Nele Vanlangenakker, Gert Van Isterdael, Karl Vergote, Riet De Rycke, Eef Parthoens, Lianne van de Laar, Takao Iwawaki, Juan R. Del Valle, Chih-Chi Andrew Hu, Bart N. Lambrecht, Sophie Janssens
NATURE CELL BIOLOGY
(2017)
Article
Multidisciplinary Sciences
Sei-ichiro Motegi, Akiko Sekiguchi, Akihiko Uchiyama, Akihito Uehara, Chisako Fujiwara, Sahori Yamazaki, Buddhini Perera, Hideharu Nakamura, Sachiko Ogino, Yoko Yokoyama, Ryoko Akai, Takao Iwawaki, Osamu Ishikawa
SCIENTIFIC REPORTS
(2017)
Article
Cell Biology
Yuichi Tsuchiya, Michiko Saito, Hiroshi Kadokura, Jun-ichi Miyazaki, Fumi Tashiro, Yusuke Imagawa, Takao Iwawaki, Kenji Kohno
JOURNAL OF CELL BIOLOGY
(2018)
Article
Dermatology
Akiko Sekiguchi, Sei-ichiro Motegi, Akihiko Uchiyama, Akihito Uehara, Chisako Fujiwara, Sahori Yamazaki, Buddhini Perera, Hideharu Nakamura, Sachiko Ogino, Yoko Yokoyama, Ryoko Akai, Takao Iwawaki, Osamu Ishikawa
JOURNAL OF DERMATOLOGICAL SCIENCE
(2018)
Article
Biology
Richard H. Chapple, Tianyuan Hu, Yu-Jung Tseng, Lu Liu, Ayumi Kitano, Victor Luu, Kevin A. Hoegenauer, Takao Iwawaki, Qing Li, Daisuke Nakada
Article
Multidisciplinary Sciences
Shiva S. Forootan, Fiona E. Mutter, Anja Kipar, Takao Iwawaki, Ben Francis, Christopher E. Goldring, B. Kevin Park, Ian M. Copple
SCIENTIFIC REPORTS
(2017)
Article
Multidisciplinary Sciences
Sechang Oh, Shoichi Komine, Eiji Warabi, Kentaro Akiyama, Akiko Ishii, Kazunori Ishige, Yuji Mizokami, Keisuke Kuga, Masaki Horie, Yoshihiro Miwa, Takao Iwawaki, Masayuki Yamamoto, Junichi Shoda
SCIENTIFIC REPORTS
(2017)
Article
Multidisciplinary Sciences
Srinath Govindarajan, Djoere Gaublomme, Renee Van der Cruyssen, Eveline Verheugen, Sofie Van Gassen, Yvan Saeys, Simon Tavernier, Takao Iwawaki, Yehudi Bloch, Savvas N. Savvides, Bart N. Lambrecht, Sophie Janssens, Dirk Elewaut, Michael B. Drennan
NATURE COMMUNICATIONS
(2018)
Article
Biochemistry & Molecular Biology
Ryosuke Nomura, Nobumasa Takasugi, Hideki Hiraoka, Yuta Iijima, Takao Iwawaki, Yoshito Kumagai, Masatake Fujimura, Takashi Uehara
Summary: Exposure to methylmercury can induce oxidative and endoplasmic reticulum stress, leading to neuronal cell death. This study investigates the time course signaling alterations of the unfolded protein response (UPR) in the most affected brain areas, revealing a potential therapeutic target for treating neuropathy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Bernardita Medel, Jose Ignacio Bernales, Alonso Lira, Dominique Fernandez, Takao Iwawaki, Pablo Vargas, Fabiola Osorio
Summary: This study aims to investigate the activation of IRE1 RNase in in vitro differentiated cDC1 cells and its functional consequences upon stimulation with viral components. The results show that several features of IRE1 activation observed in in vivo counterparts are recapitulated in optimally differentiated cDC1 cultures, and the viral analog Poly(I:C) is identified as a potent UPR inducer. The strict regulation of the IRE1/XBP1s axis plays a crucial role in cDC1 activation to viral agonists.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Hideki Hiraoka, Kengo Nakahara, Yuki Kaneko, Shiori Akiyama, Kosaku Okuda, Takao Iwawaki, Masatake Fujimura, Yoshito Kumagai, Nobumasa Takasugi, Takashi Ueharea
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2017)
Article
Clinical Neurology
Claudia Duran-Aniotz, Victor Hugo Cornejo, Sandra Espinoza, Alvaro O. Ardiles, Danilo B. medinas, Claudia Salazar, Andrew Foley, Ivana Gajardo, Peter Thielen, Takao Iwawaki, Wiep Scheper, Claudio Soto, Adrian G. Palacios, Jeroen J. M. Hoozemans, Claudio Hetz
ACTA NEUROPATHOLOGICA
(2017)