Article
Nanoscience & Nanotechnology
Yafang Shen, Fei Jia, Aoming Liang, Yawen He, Yaping Peng, Huang Dai, Yingchun Fu, Jianping Wang, Yanbin Li
Summary: A new detection modality based on immunomagnetic bead aggregation was developed for rapid and label-free detection of enrofloxacin. By exploring the mechanism of bead aggregation and investigating key parameters, a label-free biosensing platform was established for rapid and sensitive detection of enrofloxacin. Two methods were proposed for quantitatively determining enrofloxacin based on differences in magnetic separation speed and size. A 3D-printed syringe was used for automatic filtration of immunomagnetic beads. This immunosensor showed a satisfactory limit of detection and recovery rates, making it applicable to complex food samples.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Engineering, Environmental
Qi Zhang, Zhishang Li, Huang Dai, Lin Zhang, Jie Zhang, Yuanjie Liu, Jianhan Lin, Kang Liang, Yibin Ying, Yanbin Li, Yingchun Fu
Summary: Inspired by biomineralization, the study successfully grew ultrahigh load metal-organic frameworks (MOFs) on inert glass fibers, preparing hybrid membranes with high efficiency in collecting organic hazardous substances. The new method significantly enhanced the coverage and adsorption capacity of MOFs, showing exceptional robustness and maintained performance in extreme conditions.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Microbiology
Xingning Xiao, Li Bai, Sheng Wang, Lisha Liu, Xiaoyun Qu, Jianmin Zhang, Yingping Xiao, Biao Tang, Yanbin Li, Hua Yang, Wen Wang
Summary: This study investigated the sodium hypochlorite tolerance of Salmonella isolated from the poultry supply chain in China and evaluated cross-resistance. The findings showed that a high percentage of Salmonella isolates displayed resistance to multiple antibiotics and exhibited tolerance to chlorine. The presence of chlorine tolerance genes was positively correlated with the minimum inhibitory concentration levels.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Wuzhen Qi, Siyuan Wang, Lei Wang, Xinge Xi, Shangyi Wu, Yanbin Li, Ming Liao, Jianhan Lin
Summary: A portable detection device was developed for rapid, sensitive and simple detection of viable Salmonella using a finger-actuated microfluidic chip and an improved recombinase aided amplification (RAA) assay. The device could distinguish viable bacteria from dead ones by combining improved propidium monoazide (PMAxx) with RAA. It could detect Salmonella typhimurium in spiked chicken meats from 1.3 x 10 2 CFU/mL to 1.3 x 10 7 CFU/mL in 2 hours with a lower detection limit of 130 CFU/mL, and showed potential for on-site detection of foodborne pathogens.
CHINESE CHEMICAL LETTERS
(2023)
Article
Food Science & Technology
Zhoumei Huang, Zaixi Shu, Anhong Xiao, Fuwei Pi, Yanbin Li, Huang Dai, Jiahua Wang
Summary: In this study, a simple, sensitive, and specific colorimetric immunoassay based on gold nanoparticles was proposed for quantitatively detecting Aflatoxin B1 (AFB1) contamination. The method utilizing Prussian blue as a probe achieved linear detection of AFB1 concentration with a limit of 0.35 ng/mL. Acceptable recovery rates were obtained in spiked rice flour samples, showing potential for AFB1 evaluation in the food industry.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biophysics
Lei Wang, Ai Xu, Jing Yuan, Fan Jiang, Meixuan Li, Wuzhen Qi, Yanbin Li, Jianhan Lin
Summary: A hourglass-mimicking biosensor was developed using immune magnetic nanobeads for isolation, gold@platinum nanozymes for amplification, and a microplate reader/colorimetric card for determination of target bacteria. The biosensor exhibited high efficiency in separating target bacteria and achieved fast detection of bacterial concentration.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Yafang Shen, Fei Jia, Yawen He, Yingchun Fu, Weihuan Fang, Jianping Wang, Yanbin Li
Summary: Magnetic relaxation switching (MRS) biosensors are attractive in the field of food safety due to their simplicity and high signal-to-noise ratio. However, they suffer from low sensitivity and stability caused by insufficient crosslinking or non-specific binding of magnetic nanoparticles (MNPs). In this study, the CRISPR-Cas12a system was introduced into an MRS biosensor for the first time to enhance sensitivity and stability for the detection of Salmonella.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Food Science & Technology
Li Xue, Ruya Guo, Nana Jin, Siyuan Wang, Hong Duan, Wuzhen Qi, Lei Wang, Yuanjin Zheng, Yanbin Li, Jianhan Lin
Summary: This study presents a fast, sensitive, and automatic platform for Salmonella determination using continuous-flow magnetic isolation, enzymatic impedance amplification, and smartphone data analysis. The platform features fast detection, high sensitivity, and simple operation, making it promising for in-field Salmonella screening.
Article
Nanoscience & Nanotechnology
Xinyue Ma, Yue Li, Jie Zhang, Tongtong Ma, Lin Zhang, Yi Chen, Yibin Ying, Yingchun Fu
Summary: An integrated nanochannel electrode (INCE) with nanoporous gold layers was fabricated and used for small-molecule analysis. Metal-organic frameworks (MOFs) were decorated inside and outside of the nanochannels to capture small molecules and generate current signals. This study provides a new approach for nanopore analysis of small molecules by utilizing the outer surface and internal nanoconfined space of nanochannels.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Nanoscience & Nanotechnology
Jie Zhang, Zhishang Li, Qi Zhang, Lin Zhang, Tongtong Ma, Xinyue Ma, Kang Liang, Yibin Ying, Yingchun Fu
Summary: In this study, a unique nanolaminate film consisting of MXene nanosheets intercalated with MOF nanoparticles was developed. The intercalation of MOFs created nanochannels between MXene nanosheets, promoting fast water permeance. The film integrated size exclusion and selective adsorption mechanisms, enabling a rapid and selective liquid phase separation paradigm that performs simultaneous multiple chemicals and nanoparticles sieving.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Yue Li, Xinyue Ma, Wenyue Zhu, Qiao Huang, Yameng Liu, Jinming Pan, Yibin Ying, Xiahong Xu, Yingchun Fu
Summary: In this study, a novel and highly sensitive bacteria detection method was developed based on the dual-confinement effects of size and volume in a nanochannel-electrodes chip. The size confinement in the nanochannels promoted enzyme catalysis efficiency, while the volume confinement in the micro-droplet-based electrochemical cell enriched ions by restricting diffusion. This work contributes to the understanding of nanoconfined processes and provides a new signal transduction technique for trace analysis.
Article
Chemistry, Analytical
Yue Li, Yinan Zhou, Yaping Peng, Yawen He, Yafang Shen, Wen Wang, Xiangjiang Liu, Yuanjie Liu, Jianhan Lin, Yanbin Li, Yingchun Fu
Summary: This study presents a rapid and easy-to-use detection system that combines a fluorescence nanobiosensor with a homemade fluorescence analyzer for simultaneous identification and quantification of multiple antibiotics. The system demonstrated great sensitivity and accuracy in analyzing chicken samples, with significant potential for use in food safety and regulation.
ANALYTICAL METHODS
(2023)
Article
Materials Science, Multidisciplinary
Xiaobing Yang, Junjie Pan, Bingcong Xing, Wenjie Lei, Yingchun Fu, Kejun Cheng
Summary: This paper explores the degradation efficiency of AFB(1) using ZnIn2S4 as a photocatalyst. By coupling with MIL-125(Ti) and doping with La, the photocatalytic generation of superoxide radicals (O-2(-)) and hydroxyl radicals (OH) is achieved, leading to much enhanced photocatalytic performance and degradation of 97.6% of AFB(1).
MATERIALS ADVANCES
(2023)
Article
Biochemical Research Methods
Siyuan Wang, Wuzhen Qi, Shangyi Wu, Jing Yuan, Hong Duan, Yanbin Li, Jianhan Lin
Summary: This study presents an automated centrifugal system for rapid quantification of foodborne pathogenic bacteria. The system combines immunomagnetic separation for bacteria enrichment and recombinase aided amplification for nucleic acid detection. The system achieved efficient bacteria separation, nucleic acid extraction and fluorescent detection in a centrifugal disc, enabling Salmonella detection as low as 10 CFU mL(-1) in 1 hour.
