Article
Multidisciplinary Sciences
Yangyang Wu, Ting Wang, Zigao Zhao, Siyu Liu, Cong Shen, Hong Li, Mingxi Liu, Bo Zheng, Jun Yu, Xiaoyan Huang
Summary: The study utilized CRISPR/Cas9 technology to investigate the role of Rai14 in mouse testis, indicating that Rai14 is dispensable for mouse spermatogenesis and fertility. In contrast to previous results in rat models, Rai14 knockout in mice did not significantly affect sperm parameters and cellular apoptosis. The findings provide enhanced understanding of Rai14's role in the testis and may prevent redundant experiments.
Article
Cell Biology
Kehan Wang, Feifei Kong, Yuexin Qiu, Tao Chen, Jiayi Fu, Xin Jin, Youqiang Su, Yayun Gu, Zhibin Hu, Jing Li
Summary: Sertoli cells provide a nurturing environment for germ cell development and maturation, and the role of phosphatidylinositol 3-kinase (PtdIns3K) in Sertoli cells is unclear. Deleting the Pik3c3 gene specifically in Sertoli cells resulted in infertility, disrupted Sertoli cell polarity, and impaired spermiogenesis. Accumulation of SCIN caused the disorganization of the F-actin cytoskeleton, while accumulated HDAC6 deacetylated SCIN, leading to a disorganized F-actin cytoskeleton.
Article
Multidisciplinary Sciences
Yunxian Huang, Jinghua Gui, Satu-Marja Myllymaki, Marja L. Mikkola, Osamu Shimmi
Summary: This study investigates the molecular mechanisms underlying apicobasal polarity-mediated growth control in the Drosophila wing imaginal disc. The findings reveal the key role of Scribble, Septate junction complex, and alpha-Catenin in maintaining apicobasal polarity and regulating tissue growth.
Article
Andrology
Tomoki Takeda, Shoichiro Iwatsuki, Satoshi Nozaki, Atsushi Okada, Kentaro Mizuno, Yukihiro Umemoto, Takahiro Yasui
Summary: The study developed a method for observing spermatogenesis in testicular tissue without sectioning. Fluorescent labeling and multiphoton microscopy were used to identify and observe spermatogenic stages. The method showed potential for live imaging of testicular tissue.
Article
Environmental Sciences
Xi-Yu Zhang, Xiao-Fei Jiao, Di Wu, Fan Chen, Zhi-Ming Ding, Yong-Sheng Wang, Fei Meng, Ze-Qun Duan, Jia-Jun Xiong, Cai-Xia Yang, Li-Jun Huo
Summary: The study revealed that BP-3 exhibited toxicity on Sertoli cells by altering cell morphology, reducing cell viability, and disrupting the integrity of the Sertoli cell barrier. Additionally, BP-3 could perturb Sertoli cell functions through affecting junction proteins and F-actin organization, but did not induce cell apoptosis.
ENVIRONMENTAL TOXICOLOGY
(2022)
Review
Cell Biology
Mingxia Yao, Haoyang Qu, Yating Han, C. Yan Cheng, Xiang Xiao
Summary: This review summarizes recent findings on the role of Kinesin in mammalian spermatogenesis, highlighting its potential role in germ cell traversal through the blood-testis barrier and the remodeling of Sertoli cell-spermatid junctions to advance spermatid transport. The possibility of Kinesin acting as a mediator and/or synchronizer for cell cycle progression, germ cell transit, and junctional rearrangement and turnover is also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yue Feng, Dake Chen, Tiansu Wang, Jiawei Zhou, Wenning Xu, Hao Xiong, Rong Bai, Shang Wu, Jialian Li, Fenge Li
Summary: Sertoli cells contribute to the formation of the blood-testis barrier (BTB) during spermatogenesis. MicroRNA-181c/d (miR-181c/d) regulates Sertoli cell survival and barrier function by targeting the Pafah1b1 gene and inhibiting the CDC42/PAK1/LIMK1/Cofilin pathway. Excessive expression of miR-181c/d perturbs BTB function and Sertoli cell proliferation but disappears after a few weeks.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
Chao Xie, Yuxiang Jiang, Zhiwen Zhu, Shanjin Huang, Wei Li, Guangshuo Ou
Summary: The evolutionarily conserved coronin family protein POD-1 debranches Arp2/3-nucleated actin filaments in migrating neuroblasts and asymmetrically dividing embryos, contributing to cell polarity and migration. Fluorescence live imaging analysis revealed POD-1 colocalization with Arp2/3 at the leading edge of neuroblasts, indicating their synergistic role in organizing actin networks and cell polarity. Conditional mutations of POD-1 disrupted actin assembly, cell polarity, and migration, highlighting the importance of F-actin debranching in regulating these cellular processes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Anna Heinrich, Bidur Bhandary, Sarah J. Potter, Nancy Ratner, Tony DeFalco
Summary: Cdc42 is essential for Sertoli cell polarity and sperm production, as its deletion leads to increased apoptosis and disrupted polarity in the testes, ultimately affecting the progression of spermatogenesis.
Article
Agricultural Engineering
Zhi Deng, Xianghong Liu, Han Yin, Xiangyun Ji, Xiaoqing Ren, Zhihui Xia, Dejun Li
Summary: The actin cytoskeleton contributes to various cellular processes and actin-depolymerizing factor (ADF) is an important regulator of actin filament dynamics. This study investigates the role of HbADF4 in regulating actin dynamics in latex flow in rubber tree. The downregulation of HbADF4 resulted in an increase in the F/G-actin ratio in latex, while upregulation caused a decrease in the ratio. HbADF4 is also associated with stress and hormone responses in rubber tree and plays a role in cytokinesis and cell elongation.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Cell Biology
Arzu Ulu, Wonkyung Oh, Yan Zuo, Jeffrey A. Frost
Summary: Net1, an overexpressed gene in various cancers, plays a crucial role in cancer cell motility and proliferation, as well as mitotic progression. The mitotic kinase Cdk1 phosphorylates Net1 at multiple sites, negatively regulating its ability to signal to RhoA and alter actin cytoskeletal organization. The study also showed that inhibition of Cdk1 increased Net1 activity and affected its localization to the plasma membrane and cortical F-actin accumulation.
CELLULAR SIGNALLING
(2021)
Article
Neurosciences
Zhijuan Zhang, Hui Zhang, Ana Antonic-Baker, Patrick Kwan, Yin Yan, Yuanlin Ma
Summary: Epilepsy is a chronic neurological disorder associated with impaired neurodevelopment and immunity. The chemokine receptor CXCR5 plays a role in seizures through an unknown mechanism. This study found that knockdown of CXCR5 increases susceptibility to seizures in mice, while overexpression has the opposite effect. CXCR5 deficiency disrupts neuronal migration and polarization, leading to increased neuronal hyperexcitability and seizure-related electrical activity.
NEUROSCIENCE BULLETIN
(2023)
Article
Multidisciplinary Sciences
Galina Khachaturyan, Andrew W. Holle, Karen Ende, Christoph Frey, Heiko A. Schwederski, Tim Eiseler, Stephan Paschke, Alexandre Micoulet, Joachim P. Spatz, Ralf Kemkemer
Summary: Cell migration is essential for wound healing and inflammation. Temperature influences neutrophil migration, with higher temperatures increasing migration speed but also leading to increased detachment. Neutrophils can sense and respond to temperature gradients.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Prasad Vaddepalli, Thijs de Zeeuw, Soeren Strauss, Katharina Buerstenbinder, Che-Yang Liao, Joao Jacob Ramalho, Richard S. Smith, Dolf Weijers
Summary: Premitotic control of cell division orientation is critical for plant development, with formative divisions often producing daughters with different fates. Auxin-dependent division plane control involves alterations in cell geometry by regulating genes controlling cell wall and cytoskeleton properties. This interaction with cell geometry generates asymmetric divisions during the earliest steps in plant development.
Review
Cell Biology
Lingling Wang, Tiao Bu, Linxi Li, Xiaolong Wu, Chris K. C. Wong, Adolfo Perrotta, Bruno Silvestrini, Fei Sun, C. Yan Cheng
Summary: This article summarizes the research progress on the role of planar cell polarity (PCP) in supporting spermatogenesis in the testis. Recent studies have shown that PCP proteins in Sertoli cells regulate spermatogenesis by changing the organization of actin and microtubule cytoskeletons. These findings highlight the importance of PCP in supporting spermatogenesis and suggest key areas for future research.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Tiao Bu, Lingling Wang, Xiaolong Wu, Linxi Li, Baiping Mao, Chris K. C. Wong, Adolfo Perrotta, Bruno Silvestrini, Fei Sun, C. Yan Cheng
Summary: The basement membrane and apical ES laminin chains produce biologically active fragments through proteolytic cleavage, which play a crucial role in regulating cell adhesion function during spermatogenesis. These findings highlight the importance of these bioactive peptides and provide a new area of research.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Lingling Wang, Linxi Li, Xiaolong Wu, Chris K. C. Wong, Adolfo Perrotta, Bruno Silvestrini, Fei Sun, C. Yan Cheng
Summary: This article reviews recent data highlighting an interesting approach that can be considered for future studies, as well as providing mechanistic insights on the regulation of spermatogenesis.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Xiaolong Wu, Sheng Gao, Lingling Wang, Tiao Bu, Siwen Wu, Liwei Zhou, Jie Shi, Di Wu, Fei Sun, C. Yan Cheng
Summary: This review summarizes recent studies on the role of bioactive peptides as a local regulatory network in supporting spermatogenesis and analyzes transcriptome datasets of human testes to explore their potential association with azoospermia.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Editorial Material
Cell Biology
C. Yan Cheng
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Endocrinology & Metabolism
Linxi Li, Sheng Gao, Lingling Wang, Tiao Bu, Jinjin Chu, Lixiu Lv, Anam Tahir, Baiping Mao, Huitao Li, Xiaoheng Li, Yiyan Wang, Xiaolong Wu, Renshan Ge, C. Yan Cheng
Summary: Inversin is a component of the Frizzled/Dishevelled/Diversin planar cell polarity complex that plays a crucial role in tissue and organ development. Recent studies have also shown its involvement in supporting spermatogenesis in adult rat testes. This research focuses on investigating the role of inversin in Sertoli cell and testis function using RNA interference. The results demonstrate that by altering the organization of the cytoskeleton, inversin affects the barrier function of Sertoli cells and results in defects in spermatogenesis.
Review
Cell Biology
Lingling Wang, Tiao Bu, Linxi Li, Xiaolong Wu, Chris K. C. Wong, Adolfo Perrotta, Bruno Silvestrini, Fei Sun, C. Yan Cheng
Summary: This article summarizes the research progress on the role of planar cell polarity (PCP) in supporting spermatogenesis in the testis. Recent studies have shown that PCP proteins in Sertoli cells regulate spermatogenesis by changing the organization of actin and microtubule cytoskeletons. These findings highlight the importance of PCP in supporting spermatogenesis and suggest key areas for future research.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Lingling Wang, Tiao Bu, Xiaolong Wu, Sheng Gao, Xinyao Li, Angela Bryanne De Jesus, Chris K. C. Wong, Hao Chen, Nancy P. Y. Chung, Fei Sun, C. Yan Cheng
Summary: Emerging evidence suggests that cell-cell interactions, mediated by signaling proteins including FAK, Akt1/2, and Cdc42 GTPase, play a crucial role in supporting spermatogenesis. Manipulation of these signaling proteins can modify the outcome of spermatogenesis, offering potential therapeutic approaches for toxicant-induced male reproductive dysfunction.
Article
Engineering, Environmental
Yong-bing Liang, Hai-bei Li, Zheng-shan Chen, Yi-di Yang, Dan-yang Shi, Tian-jiao Chen, Dong Yang, Jing Yin, Shu-qing Zhou, Chun-yan Cheng, Yi-fan Shao, Jun-wen Li, Min Jin
Summary: The study focused on the presence and sources of intracellular and extracellular antibiotic resistance genes in chlorinated drinking water distribution systems, revealing that the eARG concentration decreased while the proportion of vanA and blaNDM-1 increased. Additionally, the diversity of the eDNA-releasing community increased with distance from the water treatment plant, and the dominant eDNA-releasing bacteria changed along the distribution system. Conversely, the concentration of intracellular ARGs increased, while the biodiversity of the planktonic bacteria community decreased along the distribution system. The findings provide important insights into the spatial behavior and sources of eARGs, highlighting health risks associated with ARGs in drinking water distribution systems.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Materials Science, Multidisciplinary
Chunyan Cheng, Xuejiao Sun, Zhiwei Yao, Chenghao Bi, Xuecheng Wei, Junxi Wang, Jianjun Tian
Summary: This study achieved balanced charge injection in QLEDs by optimizing the carrier transport layers, resulting in improved external quantum efficiency and reduced Auger recombination.
SCIENCE CHINA-MATERIALS
(2022)
Review
Cell Biology
Mingxia Yao, Haoyang Qu, Yating Han, C. Yan Cheng, Xiang Xiao
Summary: This review summarizes recent findings on the role of Kinesin in mammalian spermatogenesis, highlighting its potential role in germ cell traversal through the blood-testis barrier and the remodeling of Sertoli cell-spermatid junctions to advance spermatid transport. The possibility of Kinesin acting as a mediator and/or synchronizer for cell cycle progression, germ cell transit, and junctional rearrangement and turnover is also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Reproductive Biology
Xiaolong Wu, Liwei Zhou, Jie Shi, C. Yan Cheng, Fei Sun
Summary: This review summarizes the research findings on the etiology and pathogenesis of male infertility using multiomics approach over the past 20 years. The advanced technologies used include whole-genome bisulfite sequencing, single-cell RNA-seq, whole-exome sequencing, and transposase-accessible chromatin using sequencing.
BIOLOGY OF REPRODUCTION
(2022)
Article
Endocrinology & Metabolism
Xiaolong Wu, Damin Yun, Mengmeng Sang, Jianpeng Liu, Liwei Zhou, Jie Shi, Lingling Wang, Tiao Bu, Linxi Li, YingYing Huang, Dengfeng Lin, Fei Sun, C. Yan Cheng
Summary: This study found defects in microtubule cytoskeleton in testes of patients with azoospermia, possibly due to abnormal expression and distribution of microtubule-associated proteins. These changes may impede spermatogenesis in affected patients.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Article
Cell Biology
Tiao Bu, Xinyao Li, Lingling Wang, Xiaolong Wu, Sheng Gao, Damin Yun, Linxi Li, Fei Sun, Yan Cheng
Summary: Four-jointed box kinase 1 (Fjx1) is a Golgi-based regulator of Fat1 function by phosphorylating Fat1 at its extracellular cadherin domains. It localizes across the Sertoli cell cytoplasm and partially co-localizes with microtubules. Fjx1 knockdown perturbs Sertoli cell tight junction function, MT and actin organization, and regulates the expression of Fat1 and Dchs1.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2023)
Article
Endocrinology & Metabolism
Elizabeth Tang, C. Yan Cheng
Summary: Microtubule affinity-regulating kinases (MARKs) play an important role in the Sertoli cell blood-testis barrier, regulating cell polarity, microtubule dynamics, and tight junction permeability. MARK2 and MARK4 have different effects on the blood-testis barrier and MT cytoskeleton, with MARK4 being more severe.
Article
Biochemistry & Molecular Biology
Xiaolong Wu, Mujun Lu, Damin Yun, Sheng Gao, Shitao Chen, Longfei Hu, Yunhao Wu, Xiaorong Wang, Enkui Duan, C. Yan Cheng, Fei Sun
Summary: The study investigated the dynamic changes in chromatin organization and transcriptomes during human spermatogenesis, identifying different cell types associated with spermatogenesis and high chromatin accessibility in specific chromosomes. Analysis revealed specific transcription factor-binding sites and motifs with high frequency, as well as genes with important roles in spermatogenesis. Unique regions upstream of certain genes were shown to have high accessibility, indicating their significance in supporting human spermatogenesis.
HUMAN MOLECULAR GENETICS
(2022)
