Article
Biochemistry & Molecular Biology
Toshio Nakajima, Shun Hosoyamada, Takehiko Kobayashi, Yukio Mukai
Summary: The study reveals that APases in yeast are crucial for maintaining normal replicative lifespan. Deletion of all four APase genes shortened lifespan in yeast strains, but the issue was resolved with modification of other genes. This indicates that yeast APases modulate replicative lifespan by dephosphorylation of intracellular IP6.
Article
Biochemistry & Molecular Biology
Bart Appelhof, Matias Wagner, Julia Hoefele, Anja Heinze, Timo Roser, Margarete Koch-Hogrebe, Stefan D. Roosendaal, Mohammadreza Dehghani, Mohammad Yahya Vahidi Mehrjardi, Erin Torti, Henry Houlden, Reza Maroofian, Farrah Rajabi, Heinrich Sticht, Frank Baas, Dagmar Wieczorek, Rami Abou Jamra
Summary: This study describes eight children with Pontocerebellar hypoplasia (PCH) from four unrelated families carrying homozygous variants in the MINPP1 gene. These variants result in either a complete absence of MINPP1, impaired protein folding, destabilization of protein structure or reduction in protein stability, leading to the pathogenesis of the disease. This study presents MINPP1 as a novel autosomal recessive gene associated with PCH.
EUROPEAN JOURNAL OF HUMAN GENETICS
(2021)
Article
Oncology
Jun Deng, Xu Lin, Qi Li, Xiao-yu Cai, Lin-wen Wu, Wei Wang, Bo Zhang, Yang-ling Li, Jian Hu, Neng-ming Lin
Summary: The study revealed that INPP5B is decreased in LUAD tissues and its low expression is associated with advanced pathological features. Furthermore, INPP5B was identified as a significant independent prognostic and diagnostic factor for LUAD patients. Experimental findings demonstrated that INPP5B inhibits LUAD cell proliferation and migration by regulating the activity of the PTEN and PI3K/AKT signaling pathways.
CANCER CELL INTERNATIONAL
(2022)
Review
Immunology
Guy Tran Van Nhieu, Patricia Latour-Lambert, Jost Enninga
Summary: Shigella's type III effector IpgD plays a key role in disrupting the cortical actin filaments of epithelial cells by decreasing PI4,5P(2) levels, facilitating bacterial invasion and cell migration. Additionally, IpgD also stimulates various signaling pathways and regulates endosomal trafficking and immune responses, making it a versatile bacterial enzyme.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jia Yu, Barbara Leibiger, Shao-Nian Yang, Stephen B. B. Shears, Ingo B. B. Leibiger, Per-Olof Berggren, Christopher J. J. Barker
Summary: MINPP1 is an enzyme responsible for metabolizing inositol hexakisphosphate and inositol 1,3,4,5,6 pentakisphosphate in mammalian cells. Our study shows that cytosolic expression of MINPP1 reduces cell growth and lowers the concentration of phosphatidylinositol 3,4,5-trisphosphate. These findings highlight the importance of MINPP1's confinement to the endoplasmic reticulum.
Article
Microbiology
Huimin Huang, Yongtao Li, Dongliang Li, Li Wang, Wenqiang Jiao, Yilin Bai, Gaiping Zhang
Summary: This study reveals the role of PTPN14 in PEDV infection. PTPN14 overexpression inhibits PEDV infection, while PTPN14 knockdown or phosphatase inhibitor treatment promotes PEDV proliferation. Furthermore, PTPN14 may regulate PEDV infection by dephosphorylating STAT3 in the cytoplasm.
VETERINARY MICROBIOLOGY
(2022)
Article
Medicine, Research & Experimental
Yanbin Song, Lei Bai, Feiping Yan, Chen Chen
Summary: This study revealed the crucial role of miR-124 in glioma by targeting EMMPRIN, which led to inhibition of cell proliferation, promotion of apoptosis, and reduction of cell migration and invasion. Additionally, tumor growth was halted by miR-124 in mice, suggesting its potential as a tumor suppressor in the treatment of glioma.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Agronomy
Qian Shen, Siping Zhang, Changwei Ge, Shaodong Liu, Jing Chen, Ruihua Liu, Huijuan Ma, Meizhen Song, Chaoyou Pang
Summary: Genomic analysis of upland cotton revealed that cold tolerance was influenced by ecological distribution. The GhSAL1 gene on chromosome D09 was found to negatively regulate cold tolerance in upland cotton. Through analyzing the phenotypic and physiological parameters of cotton seedlings under chilling stress, significant genetic variations and stable QTLs associated with cold tolerance were identified. The candidate genes, including Gh_A10G0500 and Gh_D09G0189, were found to play important roles in the regulation of cold tolerance. These findings provide valuable genetic resources for improving cold tolerance in upland cotton breeding in the future.
THEORETICAL AND APPLIED GENETICS
(2023)
Article
Microbiology
Yu Tang, Ramesh Rijal, David E. Zimmerhanzel, Jacquelyn R. McCullough, Louis A. Cadena, Richard H. Gomer
Summary: Research has found that the inhibitory mechanism induced by polyphosphate can be achieved through the IP3/Ca2+ signaling pathway in Dictyostelium discoideum.
Article
Immunology
Evaristus C. Mbanefo, Ming Yan, Minkyung Kang, Sahar A. Alhakeem, Yingyos Jittayasothorn, Cheng-Rong Yu, Ashutosh Parihar, Sunanda Singh, Charles E. Egwuagu
Summary: The study developed a new nanobody SBT-100 that effectively entered human and mouse cells, suppressed STAT3 activation and lymphocyte proliferation, and inhibited Th17-mediated autoimmune diseases. In a mouse model of experimental autoimmune uveitis, SBT-100 treatment significantly reduced uveitis by suppressing expansion of pathogenic Th17 cells and rescued mice from visual impairment. The results demonstrate efficacy of SBT-100 in mice and suggest its therapeutic potential for human autoimmune diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Ren Liu, Hack Sun Choi, Yu-Chan Ko, Bong-Sik Yun, Dong-Sun Lee
Summary: In this study, 5-desmethylsinensetin was found to exhibit potential anticancer activity against breast cancer stem cells by targeting the Stat3-IL-6 and Stat3-YAP1 signaling pathways. The compound suppressed proliferation and mammosphere formation of breast cancer cells, reduced subpopulations of specific cancer cells, and inhibited expression of stemness markers.
Article
Cell Biology
Zhong-Lin Yang, Jian-Ning Chen, Yu-Yang Lu, Min Lu, Qin-Li Wan, Gui-Sheng Wu, Huai-Rong Luo
Summary: IPMK-1 protein plays a crucial role in regulating rhythmic behavior and development in C. elegans. Its expression in various tissues is essential for defecation rhythms and postembryonic growth. By dynamically regulating intracellular Ca2+ concentration, IPMK-1 and the IP3 pathway modulate rhythmic behaviors and development in C. elegans.
Article
Multidisciplinary Sciences
Gregory D. Rix, Colleen Sprigg, Hayley Whitfield, Andrew M. Hemmings, Jonathan D. Todd, Charles A. Brearley
Summary: Phylogenetic analysis, homology modelling, and biochemical methods were used to study a phytase from a Gram-negative soil bacterium. The enzyme showed stability in solution, broad pH activity profile, and specific substrate binding pockets. The transcription of the phytase gene was affected by environmental conditions.
Article
Chemistry, Medicinal
Zhen Yu, Shixin Chan, Xu Wang, Ming Wang, Rui Sun, Zhenglin Wang, Xiaomin Zuo, Jiajie Chen, Huabing Zhang, Wei Chen
Summary: The combined therapy of 5-fluorouracil (5-FU) and rutaecarpine (RUT) showed significant inhibition of proliferation, migration, and anti-apoptotic ability of colorectal cancer (CRC) cells, both in vivo and in vitro. It was found that the therapy reduced the phosphorylation of STAT3 and influenced the STAT3 signaling pathway, leading to downregulation of c-Myc, CDK4, and Bcl-2 expression while enhancing cleaved caspase-3 expression.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2023)
Article
Cell Biology
Elmira Roshani Asl, Yousef Rasmi, Behzad Baradaran
Summary: miR-124 is significantly downregulated in CRC tissues compared with normal samples, and its downregulation is negatively correlated with PD-L1 expression. Overexpression of miR-124 can inhibit Tregs differentiation by reducing PD-L1 expression, suppress CRC cell proliferation, induce apoptosis, and decrease migration and invasion capabilities of CRC cells. Additionally, miR-124 can target the STAT3 signaling pathway in CRC cells, suggesting its potential as a therapeutic target for colorectal cancer.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)