Article
Biochemistry & Molecular Biology
Olga A. Averina, Oleg A. Permyakov, Olga O. Grigorieva, Alexey S. Starshin, Alexander M. Mazur, Egor B. Prokhortchouk, Olga A. Dontsova, Petr Sergiev
Summary: Genome editing is crucial for functional genomics, but the challenge lies in the prevalence of error-prone non-homologous end joining. This study compared the efficacy of various genome editing systems on murine zygotes, finding that homologous recombination with ssDNA template following Cas9 cleavage guided by sgRNA remains the optimal method for precise genome alterations in animals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Michael G. McCoy, Daniel Perez-Cremades, Nathan Belkin, Wenhui Peng, Bofang Zhang, Jingshu Chen, Madhur Sachan, A. K. M. Khyrul Wara, Rulin Zhuang, Henry S. Cheng, Mark W. Feinberg
Summary: This study demonstrates that a 4-miRNA cassette can reprogram vascular smooth muscle cells into endothelial cells, and these induced cells exhibit high similarity to native endothelial cells. Injected induced cells even restore blood flow faster than conventional endothelial cells in a mouse model, suggesting the potential of this approach for vascular disease therapy.
Article
Oncology
Da Qin, Qingdong Guo, Rui Wei, Si Liu, Shengtao Zhu, Shutian Zhang, Li Min
Summary: This study proposed a new algorithm to quantitate plasma miRNA for colon cancer diagnosis. By establishing a miRNA-pair matrix and using various algorithms, a 4-miRNA pair model was successfully developed that showed good performance in differentiating CC from normal controls.
FRONTIERS IN ONCOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Marta Kutwin, Malwina Sosnowska, Agnieszka Ostrowska, Maciej Trzaskowski, Agata Lange, Mateusz Wierzbicki, Slawomir Jaworski
Summary: This study investigated the effect of graphene oxide (GO) complexed with antisense miRNA-21 on cytokine expression levels in U87, U118, U251, and T98 glioma cell lines. The results showed that GO-antisense miRNA-21 complexes significantly regulated the expression levels of TIMP-2, IL-6, IL-8, ICAM-1, and MCP-1 compared to electroporation of antisense miRNA-21. Furthermore, GO-mediated antisense miRNA-21 transfection resulted in upregulation of TIMP-2 and downregulation of ICAM-1, as well as significant decrease in IL-8 expression level compared to electroporation.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Nanoscience & Nanotechnology
Wei Zhang, Long Ngo, Simon Chang-Hao Tsao, Dingbin Liu, Yuling Wang
Summary: Cancer-derived sEVs can be used as a drug delivery system that targets cancer cells due to their unique features. However, the oncogenic cargo within them limits their application. We proposed an electroporation-based strategy to extract the endogenous cargo from cancer-derived sEVs, and used membrane fusion to fuse them with liposomes to form hybrid particles. These engineered hybrid particles showed improved targeting ability and higher drug loading ability, and demonstrated enhanced treatment efficiency.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Plant Sciences
Yi Ren, Yue Song, Lipeng Zhang, Dinghan Guo, Juan He, Lei Wang, Shiren Song, Wenping Xu, Caixi Zhang, Amnon Lers, Chao Ma, Shiping Wang
Summary: Peptides composed of a short chain of amino acids play important roles in plant growth, development, and stress responses, derived from precursor proteins or small open reading frames. They act as local signal molecules for intercellular communication and ligands for cellular signaling transduction. Recent studies have shown that non-coding RNAs can also encode peptides, such as miRNA-encoded peptides (miPEPs), which can increase the expression of corresponding miRNAs by enhancing MIRNA gene transcription.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemical Research Methods
Irena Jenickova, Petr Kasparek, Silvia Petrezselyova, Jan Elias, Jan Prochazka, Jana Kopkanova, Michal Navratil, Cyril Barinka, Radislav Sedlacek
Summary: The Cre-loxP recombination system is a powerful tool for genome engineering, with one application being the induction of Cre recombination in genetic mouse models. A technique of delivering Cre protein into mouse zygotes by electroporation is described as simple, affordable, and highly efficient, resulting in faster generation of desired models. Additionally, it is shown that the method is suitable for allelic conversion in primary cells derived from conditional mouse models.
Review
Biochemistry & Molecular Biology
Amrutha Menon, Noraini Abd-Aziz, Kanwal Khalid, Chit Laa Poh, Rakesh Naidu
Summary: This review focuses on the role of miRNA in cancer, therapeutic approaches with miRNAs, and effective delivery into the system. Patents and ongoing clinical trials for miRNA therapy are also summarized.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Abdul Fatah A. Samad, Mohd Farizal Kamaroddin
Summary: MicroRNA has the potential to regulate genetic pathways and has been explored as a therapeutic tool. However, there are challenges such as off-target effects, immunogenicity, and delivery platforms. Recently, innovative approaches have been developed to address these issues. Nevertheless, dosage remains an uncertainty for miRNA administration.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2023)
Article
Pharmacology & Pharmacy
Islam Saber Ead Mohamed, Aleksandra Sen'kova, Oleg Markov, Andrey Markov, Innokenty A. Savin, Marina A. Zenkova, Nadezhda L. Mironova
Summary: In this study, the mechanism of antitumor activity of bovine pancreatic RNase A was extensively investigated. The results showed that RNase A can penetrate into cells and reduce the synthesis of RNA, leading to decreased invasion and migration of tumor cells. RNase A also disrupted the favorable tumor microenvironment by altering gene expression near metastatic foci. Bioinformatic analysis revealed the main processes modulated by RNase A in the tumor microenvironment.
Article
Pharmacology & Pharmacy
Muhammad Adeel, Gloria Saorin, Giacomo Boccalon, Andrea Augusto Sfriso, Salvatore Parisi, Isabella Moro, Stefano Palazzolo, Isabella Caligiuri, Carlotta Granchi, Giuseppe Corona, Maja Cemazar, Vincenzo Canzonieri, Tiziano Tuccinardi, Flavio Rizzolio
Summary: The MAGL inhibitor MAGL23 was formulated into nanocrystals coated with albumin to improve solubility and maintain potency in cancer cell lines. The system showed enhanced therapeutic window and favorable biodistribution in in vivo studies.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Review
Plant Sciences
Jayanti Jodder
Summary: miRNAs in plants play a crucial role in controlling growth, development, and stress tolerance by modulating the expression of target genes. Modulating the expression of specific stress/development-related genes can be achieved by targeting the corresponding miRNAs. Understanding the molecular mechanism of miRNA biogenesis, particularly the processing of pri-MIRNAs, is essential for effectively modulating miRNA expression levels and ultimately improving plant yield and stress tolerance.
PLANT CELL REPORTS
(2021)
Article
Agriculture, Dairy & Animal Science
Mohammadreza Ebrahimi, Laura Mara, Bernardo Chessa, Fabrizio Chessa, Abbas Parham, Maria Dattena
Summary: Microinjection of GFP plasmid into the cytoplasm of matured oocytes or zygotes in sheep at 8-10 hours post-insemination resulted in higher proportion of GFP-positive embryos, indicating this time point as an optimized time for production of transgenic sheep.
REPRODUCTION IN DOMESTIC ANIMALS
(2021)
Article
Chemistry, Multidisciplinary
Iwona Bernacka-Wojcik, Loic Talide, Ilaria Abdel Aziz, Jan Simura, Vasileios K. Oikonomou, Stefano Rossi, Mohsen Mohammadi, Abdul Manan Dar, Maria Seitanidou, Magnus Berggren, Daniel T. Simon, Klas Tybrandt, Magnus P. Jonsson, Karin Ljung, Totte Niittyla, Eleni Stavrinidou
Summary: In this study, a flexible organic electronic ion pump was developed for controlled delivery of phytohormones in plant vascular tissue, which can induce long distance plant responses. The system provides a new tool for the study and engineering of stress-tolerant plants. The results showed that the delivery of ABA triggered stomata closure far away from the delivery point, indicating systemic vascular transport of the delivered ABA.
Review
Cell & Tissue Engineering
Seyed Mahdi Nassiri, Neda Ahmadi Afshar, Parsa Almasi
Summary: miRNAs are small non-coding RNA molecules that control gene expression by translational inhibition and mRNA destabilization, playing crucial roles in hematopoiesis. Recent studies have also confirmed the significant roles of miRNAs in cell processes such as the cell cycle, apoptosis, and differentiation, although the main contributions of some miRNAs remain unclear. Future research is needed to focus on miRNA activities during various hematopoietic stages and hematological malignancies.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Neurosciences
Min Ji Han, Won Ji Lee, Joonhyuk Choi, Yean Ju Hong, Sang Jun Uhm, Youngsok Choi, Jeong Tae Do
Summary: Adult stem cells, particularly neural stem cells (NSCs), show decreased differentiation potential with aging during long-term in vitro culture. However, introducing four reprogramming factors can inhibit NSC aging by decreasing the expression of inflammatory and ER stress-related genes. This rejuvenation method could enhance the potential of NSCs for neurological disease treatment.
JOURNAL OF COMPARATIVE NEUROLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Hyunjin Yoo, Dongyoon Choi, Youngsok Choi
Summary: Forced differentiation of mouse embryonal stem cells into lymphatic endothelial cells (LECs) using PLGA nanospheres with growth factors significantly improves yield of LYVE1(+) LECs. Enhanced LEC differentiation with VEGF-A/C-conjugated PLGA nanospheres is mediated by increased expression of genes important for lymphatic vessel formation, providing a platform for mechanistic study of LEC development in mammals.
Article
Cell Biology
Yeon Sun Kim, Seung Chel Yang, Mira Park, Youngsok Choi, Francesco J. DeMayo, John P. Lydon, Hye-Ryun Kim, Hyunjung Jade Lim, Haengseok Song
Summary: This study analyzed the phenotypes of Dgcr8 conditional knockout mice in the female reproductive tract, revealing distinct reproductive defects in Dgcr8(td/td) mice. The deficiency of Dgcr8 with different Cre systems induced overlapping but distinct phenotypes and altered the profiles of microRNAs and their target mRNAs, highlighting the importance of selecting appropriate Cre drivers for gene investigation.
CELL PROLIFERATION
(2021)
Article
Nanoscience & Nanotechnology
Hye Jin Kim, Sujin Lee, Ju Hyun Lee, Jong Min Park, Suk Jun Hong, Ok-Hee Lee, Ji Sun Park, Youngsok Choi, Keun-Hong Park
Summary: Through the study, it was found that only TRNPs could pass through the ZP layer around oocytes for drug delivery, and they did not negatively affect fertility or genetic stability.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Food Science & Technology
Do Hyun Kim, Chang Hee Jeong, Wei Nee Cheng, Hyuk Cheol Kwon, Dong-Hyeon Kim, Kun-Ho Seo, Youngsok Choi, Sung Gu Han
Summary: Kefir, a fermented milk product, is associated with reduced cancer incidence and has chemo-sensitizing effects on drug-resistant colorectal cancer cells. It attenuates multidrug resistance, increases intracellular accumulation of ROS and chemotherapeutic agents, decreases ABC transporters, and modulates upstream regulators such as ERK 1/2, JNK, and NF-kappa B. This suggests that kefir consumption may improve the effectiveness of chemotherapy in cancer patients.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Review
Biochemistry & Molecular Biology
Sohyeon Moon, Semi Hwang, Byeongseok Kim, Siyoung Lee, Hyoukjung Kim, Giwan Lee, Kwonho Hong, Hyuk Song, Youngsok Choi
Summary: In this review, the authors describe the association between the female reproductive system and the Hippo signaling pathway, as well as novel Hippo pathway genes and potential target genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Biochemistry & Molecular Biology
Byeongseok Kim, Youngsok Choi
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sohyeon Moon, Ok-Hee Lee, Byeongseok Kim, Jinju Park, Semi Hwang, Siyoung Lee, Giwan Lee, Hyukjung Kim, Hyuk Song, Kwonho Hong, Jaejin Cho, Youngsok Choi
Summary: The expression of YAP in the mouse endometrium is periodic and regulated by hormones. YAP plays an important role in the regulation of uterine endometrium dynamics through Hippo signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Hyeonji Lee, Seong-Yeob You, Dong Wook Han, Hyeonwoo La, Chanhyeok Park, Seonho Yoo, Kiye Kang, Min-Hee Kang, Youngsok Choi, Kwonho Hong
Summary: The study reveals that R-loop dynamics are associated with the regulation of zygotic genome activation (ZGA) in mice, especially in the minor ZGA stage. R-loop formation and level changes may modulate gene transcription and DNA replication.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Seonho Yoo, Eun Joo Lee, Nguyen Xuan Thang, Hyeonwoo La, Hyeonji Lee, Chanhyeok Park, Dong Wook Han, Sang Jun Uhm, Hyuk Song, Jeong Tae Do, Youngsok Choi, Kwonho Hong
Summary: This study reveals the function of the ATP-dependent chromatin remodeler Ino80 in the cell cycle regulation of embryonic stem cells (ESCs). Loss of Ino80 leads to an extended cell cycle phase and cell apoptosis. Furthermore, the regulatory mechanism of Ino80 differs slightly in differentiating ESCs. This research demonstrates that Ino80 regulates the cell cycle of ESCs through transcriptional regulation, and its function may be universal to other cell types.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Entomology
Do-Hwan Lim, Min-Seok Choi, Ji Won Jeon, Young Sik Lee
Summary: The miR-252-5p-Rab6 regulatory axis controls the Notch signaling pathway in Drosophila wing development. The loss of miR-252 increases wing size, while its overexpression leads to patterning defects in adult wings. The overexpression-induced wing phenotypes are caused by aberrant Notch signaling and intracellular accumulation of full-length Notch receptor, possibly due to defects in intracellular trafficking and autophagy-mediated degradation.
Article
Biochemistry & Molecular Biology
Sojeong Lee, Nayeon Kim, Daegyu Jang, Hee Kyung Kim, Jongin Kim, Ji Won Jeon, Do-Hwan Lim
Summary: This study found that miR-276a-3p is involved in ecdysone-mediated growth reduction in insects by regulating the expression of the Insulin-like receptor gene.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Entomology
Hee Kyung Kim, Chae Jeong Kim, Daegyu Jang, Do-Hwan Lim
Summary: In this study, the upregulation of miR-274-5p was found to activate the JNK and JAK/STAT signaling pathways by suppressing the expression of found-in-neurons (fne), which controls the formation of melanotic masses and developmental growth in flies.
Article
Biology
Chae Jeong Kim, Hyun Ho Kim, Hee Kyung Kim, Sojeong Lee, Daegyu Jang, Chanhyeok Kim, Do-Hwan Lim
Summary: The overexpression of Drosophila miR-263b in the fat body leads to a decrease in larval fat body size, resulting in severe growth defects accompanied by reduced cell proliferation and cell size. Additionally, a significant portion of larval fat body cells disassociate prematurely. This study also suggests that miR-263b-5p suppresses the expression of Laminin A (LanA), a key component of basement membranes. Overall, the findings indicate a potential role for miR-263b in developmental growth and cell association by regulating LanA expression in the Drosophila fat body.
Article
Biochemistry & Molecular Biology
Dokyun Na, Do-Hwan Lim, Jae-Sang Hong, Hyang-Mi Lee, Daeahn Cho, Myeong-Sang Yu, Bilal Shaker, Jun Ren, Bomi Lee, Jae Gwang Song, Yuna Oh, Kyungeun Lee, Kwang-Seok Oh, Mi Young Lee, Min-Seok Choi, Han Saem Choi, Yang-Hee Kim, Jennifer M. Bui, Kangseok Lee, Hyung Wook Kim, Young Sik Lee, Jorg Gsponer
Summary: This study developed a multi-layered network expansion (MLnet) model to predict common protein modifiers for a group of diseases and identified members of the insulin pathway as potential therapeutic targets. Activation of Akt1 signaling improved cell viability and memory in disease models. MLnet is a valuable tool for uncovering molecular pathways and proteins with relevance for disease groups.
MOLECULAR SYSTEMS BIOLOGY
(2023)
