Journal
RSC ADVANCES
Volume 4, Issue 109, Pages 64027-64031Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ra13646b
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Funding
- 973 Program [2011CB710806]
- National High Technology Research and Development Program of China [2012AA022201B]
- Natural Science Foundation of Zhejiang Province of China [Z4080032, R311055]
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We identified and characterized a novel halohydrin dehalogenase HHDH-PL from Parvibaculum lavamentivorans DS-1. Study of substrate specificity indicated that HHDH-PL possessed a high activity toward ethyl (S)-4-chloro-3-hydroxybutanoate ((S)-CHBE). After optimizations of the pH and temperature, whole cell catalysis of HHDH-PL was applied to the synthesis of ethyl (R)-4-cyano-3-hydroxybutyrate (HN) at 200 g L-1 of (S)-CHBE, which gave 95% conversion and 85% yield in 14 h.
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