Article
Biochemistry & Molecular Biology
Thi-Hoa Le, Ji-Hyeon Kim, Sang-Joon Park
Summary: In this study, a simple and selective fluorescence sensor for glutathione (GSH) detection was developed using nitrogen and phosphorus co-doped carbon dot-gold nanoparticle (NPCD-AuNP) composites. The composites exhibited a quenching of fluorescence intensity due to the overlap of the emission and absorption spectra. The presence of GSH led to the recovery of fluorescence intensity, which increased proportionally to the GSH concentration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Pengjuan Ni, Siyuan Liu, Yizhong Lu
Summary: The threat of glyphosate to food safety has been a topic of concern, leading to the development of a sensitive and accurate method for glyphosate detection. In this study, a turn-on fluorescent method using a PDA-PEI copolymer as a fluorescent probe and PNPP/ALP as a fluorescence quenching system was developed. The fluorescence of the copolymer depended on glyphosate concentrations, with a detection limit of 0.06μg/mL. Real samples were successfully analyzed using the standard addition method, with recoveries ranging from 88.8% to 107.0% and RSD less than 7.78%. This study provides valuable insights for glyphosate detection and expands the use of fluorescent copolymers.
Article
Chemistry, Analytical
Qiaozhen Bao, Dan Lin, Yaoran Gao, Lina Wu, Jinhua Fu, Khuslen Galaa, Xinhua Lin, Liqing Lin
Summary: A novel off-on-off fluorescent nanosensor was developed for ultra-sensitive detection of protamine and trypsin based on N,S-CDs and AuNPs. The sensor showed a good linear relationship and excellent selectivity, with detection limits of 4.7 ng/mL for protamine and 4.3 ng/mL for trypsin. The sensor was successfully applied for detection in human urine samples, demonstrating great potential in clinical diagnosis.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Yinyin Chen, Xingying She, Wei Wu, Xianxiang Wang
Summary: A dual-mode optical probe for the detection of organophosphorus pesticides triazophos (TRP) was developed based on the internal filter effect (IFE) between upconversion nanoparticles (UCNPs) and gold nanoparticles (AuNPs) in the presence of protamine (PRO) and trypsin (TRY). The probe demonstrated high sensitivity for TRP detection with good linear relationship and low detection limits. The recovery of TRP in real food samples also showed satisfactory results.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Natalia R. M. Santos, Weslley F. de Oliveira, Mariana P. Cabrera, Clovis M. Bezerra Filho, Leydianne L. S. Patriota, Thiago H. Napolea, Patricia M. G. Paiva, Maria Luiza V. Oliva, Paulo E. Cabral Filho, Adriana Fontes, Maria T. S. Correia
Summary: This study successfully conjugated the serine protease inhibitor EcTI with quantum dots (QDs) to form the QDs-EcTI nanoprobe and investigated its interaction with cancer cells. The results showed that the conjugate had a higher relative fluorescence intensity compared to bare QDs or EcTI alone, and the inhibitory activity of EcTI remained intact after interaction with QDs. Flow cytometry analysis demonstrated that the QD-EcTI conjugate could label multiple cancer cells. This study provides valuable insights into the interaction between proteases and inhibitors in cancer cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Analytical
Qingteng Lai, Yanke Zhang, Wei Chen, Zhengchun Liu
Summary: In this study, an ultrasensitive electrochemical-detection system was established for the detection of microRNA-141 (miRNA-141). A three-dimensional porous film was constructed on the electrode surface using chitosan, catechol, and nanomaterials. The system achieved a high sensitivity and stability of miRNA-141 detection by introducing ferrocene (Fc) into the electrode surface and forming a pseudocapacitor. The linear detection range was 0.5 fM to 800 pM, with a detection limit of 43.73 aM. This system overcame the limitations of traditional electroactive-ion labeling methods.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemical Research Methods
Shemsu Ligani Fereja, Zhongying Fang, Ping Li, Jinhan Guo, Tadesse Haile Fereja, Wei Chen
Summary: This study successfully synthesized highly orange-red emissive gold nanoclusters using glutathione and applied them for the detection of hemin, showing promising results and potential applications.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Tao Lu, Xiaofei Yang, Xiao-Yan Wang, Ziyong Li, Jun Yin, Sheng Hua Liu
Summary: The development of novel photochromic complexes based on dithienylethene is highly desirable for potential applications in photoresponsive optoelectronics. In this study, four novel dithienylethene-bridged gold(I) isocyanide complexes were designed and prepared, showing good photochromic behavior and unusual fluorescent switching characteristics. These complexes have the potential to be used as fluorescent switches in smart optoelectronic materials.
Article
Chemistry, Inorganic & Nuclear
Pengjun Li, Zili Li, Xirui Miao, Haoting Hou, Mao Wang, Xudong Yang
Summary: Fe-doped CPDs prepared by a one-step hydrothermal method exhibit high sensitivity and selective detection capabilities towards hydrazine hydrate, indicating good detection performance of CPDs and their composite films for N2H4 center dot H2O.
DALTON TRANSACTIONS
(2022)
Article
Spectroscopy
Chuntian Shi, Jiangxiong Luo, Yijun Wang, Ling Ding, Qingxiang Liang, Zhihui Yang, Jihao Lu, Aibin Wu
Summary: A probe 3 that selectively responds to Cr2O72- and Fe3+ was reported in this study. The probe was studied for its selectivity, pH titration, concentration titration, detection limit, time dependence, quenching constant, and recognition mechanism in a CH3CN/HEPES buffer solution. The results showed that Cr2O72- and Fe3+ could rapidly quench the fluorescence of probe 3 through the inner filter effect (IFE). Probe 3 exhibited detection limits lower than the maximum allowable concentrations in drinking water stipulated by EPA, indicating its potential as a detection tool in practical applications.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Physical
Lanjuan Sun, Chunning Sun, Yang Ge, Zhaoming Zhang, Jiancheng Zhou
Summary: Ratiometric fluorescent nanoprobes composed of upconversion nanocrystals and dye molecules were developed for the detection of hypochlorous acid (HOCl). The green upconversion luminescence (UCL) emission of the nanocrystals was effectively quenched by the dye molecules due to spectral overlap. When HOCl degraded the dye molecules, the green UCL emission was recovered while the red UCL emission remained unchanged. The developed sensor achieved a low detection limit of 1.12 mu M for HOCl and was successfully applied in tap water monitoring.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2023)
Article
Chemistry, Analytical
Lei Chen, Cheng-Pei DU, Lin Qi, Yi Wang, Kang-Lin Pei, Ren-Wen Zhang, Wen-Jing Qi
Summary: In this study, a water-soluble fluorescent azamonardine probe was prepared by reacting dopamine and resorcinol under alkaline conditions. The probe can detect Cr6+ and AA with high selectivity and sensitivity, making it suitable for accurate detection in lake water and serum samples.
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Raji V. Nair, Parvathy R. Chandran, A. Peer Mohamed, Saju Pillai
Summary: The development of selective, ultra-sensitive, rapid and facile detection methods for toxic pesticides and hazardous chemicals residues is important for food safety, environmental monitoring and public health. A fluorescent turn-on aptasensor based on sulphur-doped graphene quantum dot (S-GQD) was presented for the ultra-sensitive and selective detection of omethoate. The detection method relies on the tuning aggregation-disaggregation mechanism of S-GQD and conformational alteration of the recognition probe.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Xue Zou, Jie Hu, Huanhuan Zhu, Qiumeng Chen, Zhengjun Gong
Summary: A novel fluorescence turn-off sensing strategy was developed for I- ions detection using CDs/His-AuNCs hybrid probes. The fluorescence of the probes was effectively turned off by I- ions through internal filtration effect. Quantitative analysis of I- ions can be achieved by analyzing the fluorescence quenching degree. The established sensing platform has been successfully applied for the detection of I- ions in practical water samples. This work provides new insight into the determination of anions using fluorescent nanoparticles.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Yan Wang, Ying Yan, Xinfa Liu, Changbei Ma
Summary: This study developed a simple and fast method to detect alkaline phosphatase quantitatively, with a LOD of 0.0098 U/L. The turn-off strategy allows for the detection of alkaline phosphatase presence, making it a potential tool for quantitative detection in clinical diagnosis.