Article
Chemistry, Multidisciplinary
Jahar Dey, Sang-Jo Lee, Jiwhan Kim, Sung-Hwan Lim, Jae-Min Ha, Min-Jae Lee, Sung-Min Choi
Summary: This study introduces a method for forming nanoparticle supercrystals driven by covalent bonding interactions, which provide stronger structural stability compared to traditional intermolecular forces. The versatility and cost-effectiveness of the covalent bonding approach make it highly practical for various applications.
Article
Chemistry, Multidisciplinary
Rajeshwer Vanjari, Emad Eid, Ganga B. Vamisetti, Shaswati Mandal, Ashraf Brik
Summary: This study describes a rapid and efficient cyclization of unprotected N-propargylated peptides using Au(I) organometallic complex. By activating the propargyl functionality to form a new linkage with the N-terminus amine, the method enables cyclization of peptides with different sequences, resulting in high yields of cyclic peptides.
ACS CENTRAL SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Zhimei Huang, Xianbo Ma, Fengze Jiang, Rong Wang, Zhenkun Wu, Yi Lu
Summary: DNAwalkers have been widely used in biosynthesis, biocomputing, and biosensing due to their programmability, biocompatibility, and signal amplification capacity. A dual spatially localized DNA walker was designed to drive high-speed stochastic movement along three-dimensional tracks using bipedal catalysts. The colocalization of autocatalytic circuits and the use of bipedal catalysts significantly increased the reaction kinetics. An RNA-responsive PCHA walker was constructed for mRNA imaging in live cells, providing a novel and efficient tool for biomolecule detection and biological functions regulation.
Article
Chemistry, Applied
Wongi Jang, Jaehan Yun, Pascal N. Eyimegwu, Jian Hou, Hongsik Byun, Jun-Hyun Kim
Summary: In this study, the reduction of gold ions using mild reducing agents in the presence of poly(n-isopropylacrylamide) particles was investigated. The controlled formation of physically encapsulated gold nanoparticles was achieved through the manipulation of concentration ratios. The encapsulated gold nanoparticles showed high selectivity and the rate of reaction was influenced by the encapsulation method.
Article
Nanoscience & Nanotechnology
Zhenrui Xue, Minli You, Ping Peng, Haoyang Tong, Wanghong He, Ang Li, Ping Mao, Ting Xu, Feng Xu, Chunyan Yao
Summary: The study aimed to develop a simple and highly specific qPCR-based single-base mutation detection method, which successfully established the Taqman-MGB nanoPCR system by adding different concentrations of gold nanoparticles, improving the specificity of mutation detection with a performance of detecting mutations as low as 0.95%.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Jill Ziesmer, Isabel Sonden, Thomas Thersleff, Georgios A. Sotiriou
Summary: This study developed photothermal microneedles using new technology, reducing intradermal nanoparticle deposition and achieving efficient NIR photothermal effects, capable of causing clinically relevant hyperthermia at benign laser intensities while avoiding excessive nanoparticle deposition.
ADVANCED MATERIALS INTERFACES
(2022)
Article
Biophysics
Jeehae Shin, Sheng Li
Summary: This study investigates the interaction between DNA-conjugated molecules and lipid molecules, revealing that the morphology and amount of lipid layers formed are influenced by liposome concentration and recruiter molecule type. Surface chemistry plays a crucial role in determining the lipid layers formed, leading to multilayers on hydrophilic surfaces and monolayers on hydrophobic surfaces. The overall charge and fluidity of liposomes further modulate the total amount of lipid layers formed, providing a new approach to fine-tune lipid layer formation behavior.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Electrochemistry
Yan Song, Mingyuan Wang, Qin Qian, Jun Xu, Qungang Zhou, Shujie Lv, Peng Miao
Summary: A novel electrochemical approach has been developed in this study for the analysis of trace miRNA, which involves target triggered DNA assembly and integration of gold-nanoparticle tags to enhance electrochemical response towards miRNA. The prepared biosensor shows good analytical performance and practical utility, serving as a powerful tool for miRNA assay in biomedical fields.
Article
Chemistry, Analytical
Rokhsareh Abedi, Jahan Bakhsh Raoof, Ayemeh Bagheri Hashkavayi, Maryam Asghary
Summary: The label-free electrochemical DNA hybridization biosensor developed based on gold nanocubes modified graphite screen-printed electrodes is able to effectively detect the interaction between epirubicin and short sequence DNA of prostate cancer gene, with a linear detection range from 0.04 to 20.0 mu M and a detection limit of 0.01 mu M. The biosensor exhibits good selectivity and demonstrated applicability in human serum samples, serving as a reproducible and stable device for EPI determination.
MICROCHEMICAL JOURNAL
(2021)
Article
Multidisciplinary Sciences
Anushka Nagpal, Dinesh Dhankhar, Thomas C. Cesario, Runze Li, Jie Chen, Peter M. Rentzepis
Summary: The study investigated the effects of UV irradiation on thymine solutions using various spectroscopic methods, showing that thymine dimer formation and (6-4) photoproduct formation increase with radiation dose. Spectroscopic data revealed the mechanism of thymine dimer formation, which is known to inhibit DNA replication and cause bacterial inactivation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Pharmacology & Pharmacy
Lingyuan Xu, Xiuyuan Zhang, A. M. Abd El-Aty, Yuanshang Wang, Zhen Cao, Huiyan Jia, J. -Pablo Salvador, Ahmet Hacimuftuoglu, Xueyan Cui, Yudan Zhang, Kun Wang, Yongxin She, Fen Jin, Lufei Zheng, Baima Pujia, Jing Wang, Maojun Jin, Bruce D. Hammock
Summary: A highly sensitive fluorescence anti-quenching multi-residue bio-barcode immunoassay has been developed to detect organophosphate pesticides in fruits and vegetables. The method is stable, reliable, and able to detect low residual levels of organophosphate pesticides in agricultural products.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Chemistry, Multidisciplinary
Gregory Barbillon, Andrey Ivanov, Andrey K. Sarychev
Summary: The study focuses on maximizing surface-enhanced Raman scattering (SERS) by designing efficient substrates for chemical molecule adsorption. The hybrid asymmetric dimer array on a gold film shows significant enhancement in SERS signal for thiophenol molecules. Understanding the adsorption efficiency and constant K value is crucial for predicting SERS activity and developing sensors for various analytes.
Article
Chemistry, Physical
Marco Medves, Luca Sementa, Daniele Toffoli, Giovanna Fronzoni, Kumaranchira Ramankutty Krishnadas, Thomas Buergi, Sara Bonacchi, Tiziano Dainese, Flavio Maran, Alessandro Fortunelli, Mauro Stener
Summary: A computational study of the photo-absorption spectrum of Ag24Au(DMBT)(18) single negative anion reveals that the optical response is sensitive to the choice of xc-functionals and MPC geometry. Hybrid xc-functional in both Kohn-Sham equations and the diagonal TDDFT kernel provides consistent agreement between simulated and measured spectra.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Article
Chemistry, Analytical
Mingmin Wu, Mengtian Zhang, Zhiwei Fan, Xinyue Qin, Xiaoxia Zhu, Haiwei Ji, Yuling Qin, Qi Wang, Li Wu
Summary: Sensitive detection of DNA methyltransferase activity using a new fluorescence transducer based on Forster resonance energy transfer (FRET) is presented, with high analytical performance and capability for enzyme inhibitor screening. The method offers a promising strategy for high-performance diagnosis, drug discovery, and treatment effectiveness evaluation in various disease-related methyltransferase activities.
Article
Chemistry, Multidisciplinary
Seokjoon Kim, Ki Soo Park
Summary: Researchers have proposed a new method for nuclear staining using DNA-templated copper nanoparticles, which offers highly specific and cost-effective staining of the nucleus compared to commercial dyes. This method eliminates nonspecific signals in the cytoplasm caused by RNA and mitochondrial DNA, while also being compatible with other fluorescence-labeling techniques, showing promise as a powerful tool for cell imaging.
Article
Chemistry, Multidisciplinary
Franziska Krieg, Peter C. Sercel, Max Burian, Hordii Andrusiv, Maryna Bodnarchuk, Thilo Stoeferle, Rainer F. Mahrt, Denys Naumenko, Heinz Amenitsch, Gabriele Raino, Maksym Kovalenko
Summary: Ligand-capped nanocrystals of lead halide perovskites, particularly CsPbX3 NCs, exhibit large absorption cross section and efficient photoluminescence, making them attractive for applications in light-emitting devices and quantum optics. Size-selective precipitation of CsPbBr3 NCs coated with a long-chain sulfobetaine ligand yields monodisperse samples with tunable sizes, essential for studying their photophysics and practical applications. Additionally, zwitterion-capped NCs readily form long-range ordered superlattices with larger domain sizes and lower mosaicity, displaying superfluorescence at cryogenic temperatures.
ACS CENTRAL SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Laura Andolfi, Alice Battistella, Michele Zanetti, Marco Lazzarino, Lorella Pascolo, Federico Romano, Giuseppe Ricci
Summary: The use of scanning probe microscopies in reproductive medicine research offers new insights into cellular structures and functions, allowing for in-depth studies on gamete morphology, mechanical properties, and molecular interactions. These techniques have the potential to provide valuable information for understanding reproductive disorders and improving assisted reproductive technologies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Physical
Supriya Ghosh, Bapi Pradhan, Yiyue Zhang, Debkumar Rana, Denys Naumenko, Heinz Amenitsch, Johan Hofkens, Arnulf Materny
Summary: Increasing layers of ACI perovskites lead to more prominent multiple exciton recombination and less self-trapping of excitons. Optical anisotropy also increases with a greater number of inorganic layers. Among the three systems, ACI3 shows the most promise for photovoltaic and optical polarization devices.
JOURNAL OF PHYSICAL CHEMISTRY C
(2021)
Article
Multidisciplinary Sciences
George D. Dickinson, Golam Md Mortuza, William Clay, Luca Piantanida, Christopher M. Green, Chad Watson, Eric J. Hayden, Tim Andersen, Wan Kuang, Elton Graugnard, Reza Zadegan, William L. Hughes
Summary: The study introduces a novel digital Nucleic Acid Memory (dNAM) for high-density data storage, utilizing encoded information in DNA strands and spatial orientation for super-resolution microscopy readout. It offers long-term data retention and multi-layer error correction for emerging memory material exploration.
NATURE COMMUNICATIONS
(2021)
Article
Nanoscience & Nanotechnology
Yiyue Zhang, Masoumeh Keshavarz, Elke Debroye, Eduard Fron, Miriam Candelaria Rodriguez Gonzalez, Denys Naumenko, Heinz Amenitsch, Joris Van de Vondel, Steven De Feyter, Paul Heremans, Maarten B. J. Roeffaers, Weiming Qiu, Bapi Pradhan, Johan Hofkens
Summary: This study demonstrates high-quality two-dimensional perovskite thin films with alternating cations, which provide stable semiconductor thin films for efficient near-infrared light-emitting diodes (LEDs). By varying the thickness of the interlayer spacer cation, highly efficient LEDs with tunable emission wavelengths are achieved, showing excellent operational stability. The best-performing device exhibits high external quantum efficiency and remains stable at high current densities, indicating the potential of this novel 2D perovskite as a promising and stable photonic emitter.
Article
Cell Biology
Aleksandra Makowiecka, Ewa Mazurkiewicz, Ewa Mrowczynska, Natalia Malek, Alice Battistella, Marco Lazzarino, Dorota Nowak, Antonina Joanna Mazur
Summary: Silencing TMSB4X expression alters actin cytoskeleton organization and subcellular relocalization of intermediate filament proteins in A375 melanoma cells, leading to changes in cell topology and biomechanical properties. Despite the decrease in T beta 4 levels, not all A375 clones show reduced colony formation ability in soft agar or changes in stemness marker Nanog's expression level, indicating phenotypic heterogeneity in this cell line.
Article
Biophysics
Michele Zanetti, Suet Nee Chen, Martina Conti, Matthew R. G. Taylor, Orfeo Sbaizero, Luisa Mestroni, Marco Lazzarino
Summary: The study introduces a novel approach to improve single-cell adhesion measurement by fabricating large cantilevers and directly culturing cells on them. This method can significantly enhance cell adhesion statistics, reduce experiment time, and investigate the adhesion properties of cells that do not grow well in solution or on low adherent substrates.
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2022)
Article
Multidisciplinary Sciences
Ihor Cherniukh, Gabriele Raino, Thilo Stoeferle, Max Burian, Alex Travesset, Denys Naumenko, Heinz Amenitsch, Rolf Erni, Rainer F. Mahrt, Maryna I. Bodnarchuk, Maksym V. Kovalenko
Summary: Through precise structural engineering, perovskite nanocrystals are co-assembled with other nanocrystal materials to form a range of binary and ternary perovskite-type superlattices that exhibit superfluorescence. These ABO3 superlattices, as well as the binary NaCl and AlB2 superlattice structures that we demonstrate, exhibit a high degree of orientational ordering of the CsPbBr3 nanocubes. They also exhibit superfluorescence – a collective emission that results in a burst of photons with ultrafast radiative decay (22 picoseconds) that could be tailored for use in ultrabright (quantum) light sources.
Editorial Material
Chemistry, Physical
Luca Piantanida, William L. Hughes
Summary: In this study, data-encoded DNA sequences are stored in silica beads with barcodes for random access storage system, eliminating the need for PCR amplification.
Article
Physics, Multidisciplinary
Elias Diesen, Hsin-Yi Wang, Simon Schreck, Matthew Weston, Hirohito Ogasawara, Jerry LaRue, Fivos Perakis, Martina Dell'Angela, Flavio Capotondi, Luca Giannessi, Emanuele Pedersoli, Denys Naumenko, Ivaylo Nikolov, Lorenzo Raimondi, Carlo Spezzani, Martin Beye, Filippo Cavalca, Boyang Liu, Jorgen Gladh, Sergey Koroidov, Piter S. Miedema, Roberto Costantini, Tony F. Heinz, Frank Abild-Pedersen, Johannes Voss, Alan C. Luntz, Anders Nilsson
Summary: Using a pump-probe scheme, the study investigated the time evolution of the C K-edge x-ray absorption spectrum from CO/Ru(0001) after excitation by an ultrashort high-intensity optical laser pulse. By comparing with density functional theory spectrum calculations, they found high excitation of internal modes occurring within 200 fs, as well as thermalization of the system in the picosecond regime. The ultrafast initial excitation of vibrational modes may be attributed to the coupling of the adsorbate to nonthermalized electron-hole pairs.
PHYSICAL REVIEW LETTERS
(2021)
Review
Biochemistry & Molecular Biology
Mladenka Malenica, Marija Vukomanovic, Mario Kurtjak, Valentina Masciotti, Simone dal Zilio, Silvio Greco, Marco Lazzarino, Vedrana Krusic, Marko Percic, Ivana Jelovica Badovinac, Karmen Wechtersbach, Ivona Vidovic, Vanja Baricevic, Srecko Valic, Pero Lucin, Nika Kojc, Kristina Grabusic
Summary: Extracellular vesicles (EVs) are nanometric membranous structures secreted by cells and found in biofluids, with potential for diagnostic and prognostic purposes related to tissue states. Detailed characterisation through imaging techniques like atomic force microscopy (AFM) and electron microscopy (EM) is essential for EV usage. This study examines EV preparation protocols for AFM and EM, highlighting challenges in imaging EVs in different environments and discussing approaches for imaging with potential benefits and drawbacks. Examples using cerebrospinal fluid illustrate the complexities of EV morphology research, aiming to improve standardisation in sample preparation and microscopy methods.
Article
Nanoscience & Nanotechnology
Alessandra Milloch, Riccardo Mincigrucci, Flavio Capotondi, Dario De Angelis, Laura Foglia, Gabor Kurdi, Denys Naumenko, Emanuele Pedersoli, Jacopo S. Pelli-Cresi, Alberto Simoncig, Bjorn Wehinger, Claudio Masciovecchio, Filippo Bencivenga
Summary: This study demonstrates high-precision measurement of nanoscale elasticity in thin suspended silicon nitride membranes using extreme ultraviolet ultrashort pulse technology, providing important information on nanoscale thermal transport and behavior compatible with the diffusive regime.
ACS APPLIED NANO MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Nicoletta Braidotti, Maria Augusta do R. B. F. Lima, Michele Zanetti, Alessandro Rubert, Catalin Ciubotaru, Marco Lazzarino, Orfeo Sbaizero, Dan Cojoc
Summary: This study investigates the relationship between cytoskeleton alterations and diseases through examining primary rat cardiac fibroblasts. The results show that changes in the cytoskeleton can affect cell adhesion, rheological behavior, and morphology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Martina Conti, Ilaria Bolzan, Simone Dal Zilio, Pietro Parisse, Laura Andolfi, Marco Lazzarino
Summary: Cell migration plays a crucial role in physiological and pathological contexts, but its mechanisms are still not fully understood due to the complex interaction between cells and their surroundings. Traditional assays lack the ability to capture this complexity, while a novel approach using 2D micro-patterned substrates provides a promising tool to investigate cell migration in complex environments. The developed method was tested with breast cancer cell lines and revealed that invasive cells are more responsive to the size of the water meniscus and exhibit significative matrix deposition process connected to cell migration.
Article
Biochemistry & Molecular Biology
Domenico Tierno, Eros Azzalini, Rossella Farra, Sara Drioli, Fulvia Felluga, Marco Lazzarino, Gabriele Grassi, Barbara Dapas, Serena Bonin
Summary: Epithelial ovarian cancers (EOCs) are a heterogeneous group of tumors with different molecular and clinical features. Few improvements have been achieved in terms of EOC management and treatment efficacy, and the 5-year survival rate of patients remained almost unchanged. A better characterization of EOCs' heterogeneity is needed to identify vulnerabilities, stratify patients, and adopt proper therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)