Article
Chemistry, Multidisciplinary
Brena L. Thompson, Ian A. Kieffer, Zachariah M. Heiden
Summary: This report describes the implementation of a BODIPY dye into a borane ligand framework, resulting in a family of molecular boranes with tunable Lewis acidities through BODIPY-based redox events.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Physical
Adedayo Kukoyi, Hongshan He, Kraig Wheeler
Summary: BODIPYs at different positions of quinolines show similar absorption spectra but very different visible emission efficiency. Single-crystal structures indicate different tilting angles of quinoline groups and BODIPY core structure, which may be responsible for the gradual change in fluorescence through photoinduced electron transfer.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Heng Li, Fan Lv, Xing Guo, Qinghua Wu, Hao Wu, Bing Tang, Changjiang Yu, Hua Wang, Lijuan Jiao, Erhong Hao
Summary: A new oxidative nucleophilic alpha-hydrogen substitution reaction method has been developed for efficient functionalization of BODIPY, leading to a series of alpha-alkoxylated BODIPY compounds. Furthermore, a new mitochondria-targeting fluorescent probe was successfully designed using this method.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Organic
Anatolii Sokolov, Maria A. Sycheva, Dilara A. Farkhutdinova, Ivan N. Myasnyanko, Andrey A. Mikhaylov, Nadezhda S. Baleeva, Anastasia Bochenkova, Mikhail S. Baranov
Summary: This study finds that pyridineacetic acids can undergo dimerization under the action of activating agents, resulting in the formation of 2-hydroxy-3-(pyridin-2-yl)-4H-quinolizin-4-ones. Introducing a difluoroboryl bridge into these derivatives leads to the development of novel highly fluorescent compounds.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Chemistry, Analytical
Wen-Jing Shi, Xu-Hui Yan, Jinrong Yang, Yong-Feng Wei, Yi-Tong Huo, Cai-Ling Su, Jin-wu Yan, Dongxue Han, Li Niu
Summary: In this study, we reported four meso-five-membered heterocycle-substituted BODIPY-based fluorescent probes with viscosity-responsive and AIE properties, and organelle-targeting ability. Among them, probe 1 with a meso-thiazole group showed good viscosity-responsive and AIE properties, successfully targeting mitochondria and lysosomes for imaging cellular viscosity changes. Probe 3 with a meso-benzothiophene group only exhibited viscosity-responsive properties without subcellular localization. Probe 2 showed AIE phenomenon without obvious viscosity-responsive property, while probe 4 displayed fluorescence quenching in polar solvents. Our findings provide insights into the structure-property relationships of these fluorescent rotors and highlight probe 1 as a sensitive AIE fluorescent rotor for imaging dual-organelle viscosity.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Fentahun Wondu Dagnaw, Yi-Ping Cai, Qin-Hua Song
Summary: In this study, three meso-substituted BODIPY piperazines were prepared for the rapid and sensitive detection of nerve agent simulants. Among them, BPA-2 showed the best fluorescence sensing performance with the lowest detection limits. Polymer test strips embedded with BPA-2 exhibited rapid response, high sensitivity, and selectivity for detecting nerve agents in gas phase.
Article
Optics
Binyu Zhao, Linhong Liao, Yuyan Zhu, Zhiyuan Hu, Fengshou Wu
Summary: This review summarizes the latest advancements in Aza-BODIPY dyes in biomedical imaging, with a focus on their versatile applications in cancer diagnoses, organ injuries, and surgical navigation. The regulation strategies for the photophysical properties of Aza-BODIPY dyes are discussed, and the future challenges and development prospects in the biomedical field are presented. This article aims to provide meaningful inspiration for the further development of functional organic dyes with excellent properties.
JOURNAL OF LUMINESCENCE
(2023)
Article
Chemistry, Medicinal
Amaury Guillou, Eda Nisli, Simon Klingler, Anthony Linden, Jason P. Holland
Summary: Fluorescent protein conjugates are important tools in scientific research, and the synthesis of photoactivatable fluorophores provides a potential approach for protein labeling and imaging.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Review
Chemistry, Organic
Liu Bin-Kai, Teng Kun-Xu, Niu Li-Ya, Yang Qing-Zheng
Summary: This review discusses the modification strategies of boron dipyrromethene (BODIPY) dyes at different reaction positions, as well as the Pd-catalyzed cross-coupling reactions and oxidative coupling reactions of BODIPY. The development prospects of this field are also explored.
CHINESE JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Chemistry, Applied
Xiaoqing Wang, Fen Qi, Zhiyong Jiang, Ming Yan, Li Xu
Summary: The newly developed family of pyrimidyl-enolate-based bisboron complexes, DB1-3, exhibit intense fluorescence in both solution and solid-state with high quantum yields. Despite their propeller-shaped structure, these complexes display strong fluorescence in diluted solution due to efficient steric hindrance induced by phenyl rings, as well as highly efficient solid-state fluorescence.
Article
Chemistry, Multidisciplinary
Paramjit Kaur, Kamaljit Singh
Summary: The development of selective and sensitive chemical sensors for detecting various analytes has attracted significant interest. The timely detection of heavy metal ions, toxic anions, and explosives is crucial for ensuring safety and security. In this personal account, the authors present their designed molecular sensors for detecting different species in various media. They demonstrate the modulation of optical properties for selective and sensitive detection and extend the application of these probes to on-site detection.
Article
Chemistry, Multidisciplinary
Denis Y. Uvarov, Sergey A. Gorbatov, Marya K. Kolokolova, Mikhail A. Kozlov, Natalya G. Kolotirkina, Igor V. Zavarzin, Christine Goze, Franck Denat, Yulia A. Volkova
Summary: Novel derivatives of BODIPY functionalized at the meso-position with polyamines and polyoxyethylenes were synthesized through a single-step condensation reaction. Spectroscopic characterization of the stable dyes bearing polyoxyethylene substituents was carried out, and one compound showed promise for UV-Vis and off-on fluorescence Cu2+ detection, selectively detecting Cu2+ ions in the presence of other metal cations.
Article
Spectroscopy
Xiangying Sun, Lifen Cai, Wei He, Xuegong Cao, Bin Liu, Huaiqian Wang
Summary: This paper studied two tetrahydo[5]helicene-based dyes and synthesized water-soluble blue carbon dots. It was found that these dyes and carbon dots combined could be used for sensitive detection of water content in ethanol, with good accuracy and precision. Additionally, the ratiometric probes showed good reversibility in thermometry based on optical temperature sensors.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Elizabeth M. Santos, Wei Sheng, Rahele Esmatpour Salmani, Setare Tahmasebi Nick, Alireza Ghanbarpour, Hadi Gholami, Chrysoula Vasileiou, James H. Geiger, Babak Borhan
Summary: Fluorescent proteins have great potential in revolutionizing biology, with engineered design strategies allowing for high quantum yield, red-shifted emission, and Large Stokes Shift. This technology shows immense potential for applications in biological research.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
Zhihui Qin, Weiwei Su, Ping Liu, Jinming Ma, Yaru Zhang, Tifeng Jiao
Summary: Iron ions play a crucial role in our lives, but both excessive and insufficient intake can lead to diseases. The environment and human health can be easily contaminated by these metal ions due to industrialization. Therefore, the development of methods for detecting iron ions is vital. In this study, a RhB-EDA fluorescent probe was prepared for the high recognition of Fe3+, achieving dual spectral detection and naked-eye observation.
Article
Biochemistry & Molecular Biology
Beatriz Lozano-Torres, Juan F. Blandez, Alba Garcia-Fernandez, Felix Sancenon, Ramon Martinez-Manez
Summary: This study describes a new method for senescent cell detection based on lipofuscin labeling using a biorthogonal reaction. The method involves a two-step reaction that forms a clear fluorescence pattern in senescent cells, providing an alternative tool for senescent cell detection.
Article
Chemistry, Multidisciplinary
Antoni Llopis-Lorente, Bastiaan C. Buddingh', R. Martinez-Manez, Jan C. M. van Hest, Loai K. E. Abdelmohsen
Summary: This article demonstrates population behavior in microorganisms based on quorum sensing communication. Researchers utilized giant lipid vesicles loaded with sender-receiver machinery (enzymes and responsive biomolecules) to achieve quorum sensing behavior in an artificial cell population. The system allows for the examination of collective output based on cell density, fuel concentration, and proximity, which are important factors controlling natural quorum sensing behavior.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Marcia Dominguez, Kathleen Meyer, Felix Sancenon, Juan F. Blandez, Manuel Serrano, Ramon Martinez-Manez
Summary: Kidney damage can be monitored using different biomarkers in blood, urine or serum, and kidney failure leads to the overexpression of the alanine aminopeptidase (APN) enzyme in urine. In this study, a molecular probe (NB-ALA) based on the Nile Blue fluorophore (NB) was designed to detect APN enzyme in urine through simple fluorometric measurements.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Amelia Ultimo, Mar Orzaez, Maria J. Santos-Martinez, Ramon Martinez-Manez, Maria D. Marcos, Felix Sancenon, Eduardo Ruiz-Hernandez
Summary: The abnormal expression of vascular endothelial growth factor (VEGF) in the retinal pigment epithelium (RPE) is the main cause of subretinal neovascularisation in wet age-related macular degeneration (AMD). Encapsulating anti-VEGF drugs in large pore mesoporous silica nanoparticles (LP-MSNs) provides a potential solution for the issues in current AMD treatment, by improving drug penetration, retention time, and sustained release. In this study, an innovative LP-MSN-based nanosystem, functionalized with polyethylenimine (PEI) chains, is developed for topical administration of anti-VEGF siRNA molecules to RPE cells, demonstrating successful VEGF silencing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Alejandra Estepa-Fernandez, Alba Garcia-Fernandez, Araceli Lerida-Viso, Angela Morella-Aucejo, Juan Jose Esteve-Moreno, Juan F. Blandez, Maria Alfonso, Vicente Candela-Noguera, Gema Vivo-Llorca, Felix Sancenon-Galarza, Mar Orzaez, Ramon Martinez-Manez
Summary: The engineering of nanoparticle communication has gained attention, but communication between nanoparticles and living systems is still understudied. This study explores a strategy that involves nanoparticle-cell-nanoparticle communication through modifying the environment. The targeted therapy through stigmergy communication effectively inhibits tumor growth and reduces metastases in a mouse model of triple-negative breast cancer with minimal side effects.
Article
Pharmacology & Pharmacy
Alejandra Estepa-Fernandez, Alba Garcia-Fernandez, Araceli Lerida-Viso, Juan F. Blandez, Irene Galiana, Felix Sancenon-Galarza, Mar Orzaez, Ramon Martinez-Manez
Summary: Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer with limited treatment options. Inducing senescence and removing senescent cells (senolysis) has been explored as a method to limit tumor progression. In this study, a combination treatment using palbociclib and navitoclax was found to delay tumor growth and reduce metastasis in a mouse model of aggressive TNBC.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Chemistry, Analytical
Eva Garrido, Guillermo Hernandez-Siguenza, Estela Climent, M. Dolores Marcos, Knut Rurack, Pablo Gavina, Margarita Parra, Felix Sancenon, Vicente Marti-Centelles, Ramon Martinez-Manez
Summary: The use of gamma-hydroxybutyric acid (GHB) in drug-facilitated sexual assault has increased, leading to the need for rapid GHB detection methods. A portable strip-based lateral flow assay (LFA) using a smartphone for fluorescence readout was developed for GHB analysis. This method is based on a Cu2+ complex with a tetradentate ligand and the fluorescent dye coumarin 343, and can detect GHB at concentrations as low as 0.03 μM in MES buffer solution and 0.1 μM in soft drinks and alcoholic beverages in just 1 min.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Engineering, Biomedical
Andy Hernandez-Montoto, M. Nieves Aranda, Isabel Caballos, Alba Lopez-Palacios, Maria Angeles Tormo-Mas, Javier Peman, Mireya Prieto Rodriguez, Carlos Picornell, Elena Aznar, Ramon Martinez-Manez
Summary: Fluorogenic probes based on oligonucleotide capped nanoporous anodic alumina films were developed for specific and sensitive detection of human papilloma virus (HPV) DNA. The probes showed high sensitivity and selectivity in detecting 14 different high-risk (hr) HPV types in clinical samples. The optimized synthesis protocol allowed for the production of sensors with high reproducibility and reliable fluorescence signal reading.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Isabel Caballos, Maria Nieves Aranda, Alba Lopez-Palacios, Luis Pla, Sara Santiago-Felipe, Andy Hernandez-Montoto, Maria Angeles Tormo-Mas, Javier Peman, Maria Dolores Gomez-Ruiz, Eva Calabuig, Beatriz Sanchez-Sendra, Clara Frances-Gomez, Ron Geller, Elena Aznar, Ramon Martinez-Manez
Summary: The COVID-19 pandemic has emphasized the importance of testing and tracking infected individuals to mitigate the virus spread. This study introduces a biosensor that uses oligonucleotide-gated nanomaterials for specific detection of the SARS-CoV-2 spike protein. The biosensor showed high sensitivity and successfully detected low concentrations of the pseudotype virus. It was also tested on nasopharyngeal samples from suspected COVID-19 individuals.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Pharmacology & Pharmacy
Cristina de la Torre, Carmen Coll, Amelia Ultimo, Felix Sancenon, Ramon Martinez-Manez, Eduardo Ruiz-Hernandez
Summary: A novel combination of in situ-forming hydrogels and gated mesoporous materials was developed for designing depots that can locally sustainably release chemotherapeutics. The depot is composed of a hyaluronic-based gel loaded with redox-responsive mesoporous silica nanoparticles capped with polyethylene glycol chains containing a disulfide bond. The nanoparticles are able to deliver the cargo in the presence of the reducing agent glutathione, promoting pore opening and cargo delivery. Release studies and cellular assays demonstrated the successful liberation of the nanoparticles and their internalization into cells, leading to significant reduction in cell viability when loaded with doxorubicin. This research opens up possibilities for the development of new depots combining the tunable properties of hyaluronic gels with a wide range of gated materials for enhanced local controlled release of chemotherapeutics.
Article
Biotechnology & Applied Microbiology
Jose Manuel Terres-Haro, Javier Monreal-Trigo, Andy Hernandez-Montoto, Francisco Javier Ibanez-Civera, Rafael Masot-Peris, Ramon Martinez-Manez
Summary: In this study, a Finite Element Model is used to calculate the absorption and electric field enhancement of metal nanoparticles in an optical, electromagnetic field. The temperature rise caused by irradiating a suspension of nanoparticles was also measured and compared with experimental results.
BIOENGINEERING-BASEL
(2023)
Article
Chemistry, Analytical
Oscar Nieves, David Ortiz de Zarate, Elena Aznar, Isabel Caballos, Eva Garrido, Ramon Martinez-Manez, Fabian Dortu, Damien Bernier, Beatriz Mengual-Chulia, F. Xavier Lopez-Labrador, Jens J. Sloth, Katrin Loeschner, Lene Duedahl-Olesen, Natalia Prado, Martin Hervello, Armando Menendez, Rainer Gransee, Thomas Klotzbuecher, M. Clara Goncalves, Fahimeh Zare, Ana Fuentes Lopez, Isabel Fernandez Segovia, Jose M. Barat Baviera, Jaime Salcedo, Sara Recuero, Santiago Simon, Ana Fernandez Blanco, Sergio Peransi, Maribel Gomez-Gomez, Amadeu Griol
Summary: This paper presents a concept for a novel adaptable sensing solution developed under the PHOTONGATE project. The solution allows for quantification of multiple analytes, with high sensitivity and selectivity. It relies on biochemical and photonic technologies and has potential applications in health diagnosis and food safety sectors.
Article
Engineering, Biomedical
Alba Garcia-Fernandez, Monica Sancho, Eva Garrido, Viviana Bisbal, Felix Sancenon, Ramon Martinez-Manez, Mar Orzaez
Summary: Acute lung injury (ALI) is a severe pulmonary disorder with high mortality and morbidity rates in intensive care unit patients. The activation of NLPR3 inflammasome during ALI leads to the release of proinflammatory factors and pyroptosis, contributing to lung damage. In this study, it is shown that inhibiting ASC oligomerization through MM01 compound can be a potential therapy for ALI. Additionally, using nanoparticles designed to target the inflamed lungs enhances the efficacy of the treatment.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Irene Barguilla, Vicente Candela-Noguera, Patrick Oliver, Balasubramanyam Annangi, Paula Diez, Elena Aznar, Ramon Martinez-Manez, Ricard Marcos, Alba Hernandez, Maria Dolores Marcos
Summary: This study evaluated the genotoxicity and transforming ability of different functionalized mesoporous silica nanoparticles on human lung epithelial cells. The results showed that functionalized mesoporous silica nanoparticles induced some measurable adverse effects associated with tumorigenesis, but the toxicity was lower compared to other nanomaterials and certain drugs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Applied
Vicente Candela-Noguera, Maria Alfonso, Pedro Amoros, Elena Aznar, Maria Dolores Marcos, Ramon Martinez-Manez
Summary: This study comprehensively investigates the factors affecting the formation of MCM-41 type mesoporous silica nanoparticles (MSN). The hydrolysis and condensation rates are found to play a central role in nanoparticle formation, and disregarded parameters such as stirring strength and surfactant addition rate are shown to modulate nucleation and growth. Furthermore, the aging and evolution of MSN are studied through the analysis of silica framework consolidation over time, providing insights into obtaining reproducible, homogeneous, and tailored-made MCM-41 type MSN.
MICROPOROUS AND MESOPOROUS MATERIALS
(2024)
