Article
Spectroscopy
Haifei Wang, Panpan Wang, Liangfeng Niu, Chunhui Liu, Yingzhe Xiao, Ying Tang, Yao Chen
Summary: A novel fluorescent probe based on carbazolethiophene was developed for specific Cu2+ detection in living cells. The probe exhibits rapid response, high sensitivity, and good cell-membrane permeability, with strong fluorescence in aqueous media. Live cell studies demonstrate successful visualization of intracellular Cu2+ concentration fluctuations.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Spectroscopy
Boxin Zhou, Beibei Wang, Mengqi Bai, Mingdong Dong, Xu Tang
Summary: Cysteine plays an important role in the human body, and abnormal concentration can lead to various diseases. Detecting cysteine in vivo with high selectivity and sensitivity is therefore significant. In this study, a small organic fluorescent probe ZHJ-X was designed and synthesized based on cyanobiphenyl, which specifically recognized cysteine. The probe ZHJ-X demonstrated specific selectivity, high sensitivity, short response time, good anti-interference ability, and a low detection limit of 3.8 x 10-6 M. It was successfully applied for visualizing cysteine in living cells and holds potential for cell imaging and detection.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Biochemistry & Molecular Biology
Lan Fang, Wen Shao, Shu-Tang Zeng, Gui-Xue Tang, Jia-Tong Yan, Shuo-Bin Chen, Zhi-Shu Huang, Jia-Heng Tan, Xiu-Cai Chen
Summary: In this study, a new set of styryl probes were designed and synthesized to improve the detection limit and selectivity of RNA imaging. QUID-2, among these probes, showed the most promising results. It exhibited excellent photostability and low cytotoxicity, and could reveal the global dynamics of RNA in live cells. Moreover, QUID-2 was found to have potential for detecting RNA granules in live cells.
Article
Chemistry, Analytical
Wei Wang, Min Ji, Junqing Chen, Peng Wang
Summary: In this study, a turn-on type aggregation-induced emission (AIE) fluorescent probe BQM-NBD was developed for selective detection of Cys and Hcy in organisms, showing good selectivity. Additionally, BQM-NBD exhibited low cytotoxicity when used for imaging Cys in living cells.
Article
Chemistry, Analytical
Chen Xu, Yukun Zhang, Hui Sun, Jindong Ai, Mingguang Ren
Summary: We have developed a new two-photon fluorescent probe (T-HS) for the detection of H2S. The probe exhibits regular changes in absorption and fluorescence spectra upon addition of hydrogen sulfide. It demonstrates advantageous properties such as a large turn-on fluorescence signal, good selectivity, and low cytotoxicity. Furthermore, the probe T-HS has been successfully utilized for fluorescence imaging of H2S in live cells and zebrafish.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Yaoyun Hu, Zhuye Shang, Juan Wang, Min Hong, Run Zhang, Qingtao Meng, Zhiqiang Zhang
Summary: A phenothiazine-based fluorescent probe (PR) has been developed for the selective detection of hydrogen sulfide (H2S) in biosystems and monitoring H2S produced in the food spoilage process. PR showed high selectivity and sensitivity, low cytotoxicity, and reliability at physiological pH. The probe was successfully applied for the visualization of exogenous and endogenous H2S in living cells and live animals, as well as for monitoring H2S production in an inflammation model.
Article
Spectroscopy
Yongpeng Wang, Jia Chen, Yang Shu, Jianhua Wang, Hongdeng Qiu
Summary: In this study, a fluorescent probe TNP-SBD-Cl was developed to specifically identify cysteine (Cys) and avoid interference from other biological analytes. The probe can monitor Cys in living HeLa cells with low cytotoxicity, showing significant diagnostic potential in physiological and pathological processes.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Spectroscopy
Liuqi Qiao, Yongxing Yang, Jianhua Cai, Xin Lv, Junsheng Hao, Yaping Li
Summary: The fluorescent probe PA-A shows high selectivity, sensitivity, and low detection limit for detecting Cys, with the added benefit of being visible to the naked eye. Fluorescence imaging experiments in cells successfully demonstrated excellent tissue penetration.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Spectroscopy
Qingqing Ma, Wenfeng Zhuo, Zhaodong Zhai, Guangshuai Gong, Tian Zhang, Haibin Xiao, Ziyan Zhou, Yuying Liu
Summary: The newly designed pH-sensitive fluorescent probe NAP-MDA can exhibit different fluorescence signals according to changes in acidity and alkalinity. It demonstrates excellent selectivity and linear dependence with pH, enabling fluorescence imaging and quantitative analysis of pH in live cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Jingran Zhang, Lele Liu, Yanan Wang, Chao Wang, Yurong Guo, Zihan Yuan, Yan Jia, Peng Li, Shuqing Sun, Guangjiu Zhao
Summary: Endogenous peroxynitrite plays a crucial role in regulating life activities, but the existing tools for ONOO- tests are inadequate. We have developed a fluorescent probe TPA-F-NO2, based on the D-pi-A structure, for imaging endogenous ONOO- in living cells, which exhibits a low fluorescence background in water. TPA-F-NO2 enables naked eye detection of ONOO- through noticeable color changes. It possesses several advantages, such as a large stokes shift, high signal-to-noise ratio, and high selectivity and sensitivity, allowing for quantification of ONOO- in the concentration range of 0-14 μM. Its solvatochromic characteristics make TPA-F-NO2 a potential candidate for applications in OLEDs and other fields. Additionally, by assembling TPA-F-NO2 and 4-methylumbelliferone, we have prepared TPA-MU-NPs, which not only possess ONOO- imaging capabilities but also exhibit anti-proliferative effects on breast cancer cells and other cells. This 'probe-drug' assembly strategy offers new insights for the design and optimization of dual-functional probes.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Applied
Xiaokun Yang, Jiamin Wang, Zunlong Zhang, Bo Zhang, Xiaolin Du, Jian Zhang, Jianhong Wang
Summary: In this study, a BODIPY-based turn-on fluorescent probe BDP-S was designed and synthesized for detecting the semi-essential amino acid cysteine (Cys). The probe exhibited fast response (10 min), distinct color change (from blue to pink), high signal-to-noise ratio (3150-fold), and high selectivity and sensitivity (LOD = 11.2 nM) towards Cys. Furthermore, BDP-S could be used for quantitative determination of Cys in food samples and qualitative detection of Cys on test strips. Importantly, BDP-S was successfully applied for imaging Cys in living cells and in vivo. Therefore, this work provides a potentially powerful tool for detecting Cys in food samples and complex biological systems.
Article
Chemistry, Applied
Jianning Dong, Yanhua Wang, Congbin Fan, Yayi Tu, Shouzhi Pu
Summary: A fluorescent probe Lyso-CDC based on chalcone fluorescent dyes was designed and synthesized for the effective detection of cysteine (Cys) in lysosomes. The probe showed significantly enhanced fluorescence with a large Stokes shift and visible color change upon the addition of Cys. It exhibited higher selectivity and sensitivity towards Cys compared to other amino acids, with a detection limit of 1.44 x 10-8 M. The probe also demonstrated low cytotoxicity, excellent biocompatibility, and remarkable lysosome targeting ability, enabling successful detection of Cys in living cell lysosomes.
Article
Chemistry, Analytical
Ruo-Jun Man, Meng-Ke Wu, Bing Yang, Yu-Shun Yang
Summary: In this work, a novel fluorescent probe TZPzine-1 was developed for selective detection of hydrazine in water samples and living cells, showing high sensitivity, wide linear range, and high selectivity. The probe also demonstrated rapid response and practical stability under various pH and temperature conditions.
Article
Biochemistry & Molecular Biology
Wei Wang, Zihao Peng, Min Ji, Junqing Chen, Peng Wang
Summary: In this study, a fluorescent probe SQM-NBD was synthesized for selective detection of Cys/Hcy. The probe showed superior sensitivity to Cys/Hcy and was able to achieve fluorescence imaging of Cys/Hcy in HeLa cells, demonstrating its potential for Cys/Hcy identification under physiological and pathological conditions.
BIOORGANIC CHEMISTRY
(2022)
Article
Spectroscopy
Shuwei Zhang, Wenyi Liao, Xuewen Wang, Xinyao Wang, Ting Wang, Yu Yuan, Gang Chen, Xiaodong Jia
Summary: A novel fluorescent probe based on indanone was developed for effective detection of Cys and Hcy. The probe showed weak fluorescence but exhibited obvious enhancement when reacted with Cys/Hcy. It demonstrated good anti-interference and low cytotoxicity, making it suitable for monitoring Cys and Hcy in living cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
