Article
Chemistry, Analytical
Wei Wang, Min Ji, Junqing Chen, Peng Wang
Summary: In this study, a turn-on type aggregation-induced emission (AIE) fluorescent probe BQM-NBD was developed for selective detection of Cys and Hcy in organisms, showing good selectivity. Additionally, BQM-NBD exhibited low cytotoxicity when used for imaging Cys in living cells.
Article
Spectroscopy
Nadeem Ahmed, Wajeeha Zareen, Di Zhang, Xiaopeng Yang, Yong Ye
Summary: A novel fluorescent ON probe NC-Cu was developed to trace minute levels of Cu2+ ions in the presence of various biological active species. The probe exhibited remarkable fluorescence enhancement and showed an excellent linear relationship with a detection limit of 5.8 μM for Cu2+ ions. Furthermore, NC-Cu was effectively functional in living cells to trace Cu2+ ions.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Applied
Yu-Chao Feng, Zhi-Bin Cai, Sheng-Li Li, Li-Jun Chen, Qing Ye, Yu-Peng Tian
Summary: A series of V-shaped donor-n-acceptor-n-donor compounds with an aldehyde group were designed, synthesized, and evaluated as two-photon excited fluorescence probes for selective detection of cysteine. The structures were characterized and their electronic structures were studied. Two compounds, 1Pip and 3TEG, showed high selectivity, good anti-interference ability, and favorable two-photon absorption performance for detecting cysteine. Cell fluorescence imaging experiments showed that 3TEG had better cellular membrane permeability and could visualize endogenous and exogenous cysteine in living cells. These results suggest that 3TEG has potential as a two-photon fluorescence probe for detecting cysteine in biological samples.
Article
Biochemistry & Molecular Biology
Wei Wang, Zihao Peng, Min Ji, Junqing Chen, Peng Wang
Summary: In this study, a fluorescent probe SQM-NBD was synthesized for selective detection of Cys/Hcy. The probe showed superior sensitivity to Cys/Hcy and was able to achieve fluorescence imaging of Cys/Hcy in HeLa cells, demonstrating its potential for Cys/Hcy identification under physiological and pathological conditions.
BIOORGANIC CHEMISTRY
(2022)
Article
Chemistry, Physical
Ishani Bose, Yan Zhao
Summary: A nanoparticle catalyst with a catalytic motif similar to aspartic proteases was reported, capable of generating a hydroxide ion under acidic conditions and accelerating the hydrolysis of para-nitrophenyl acetate. The catalytic efficiency was controlled by the flexibility of the catalytic groups in the active site, and the synthetic enzyme could detect structural changes in the substrate.
Article
Spectroscopy
Baowen Zhang, Lianfang Chen, Maosen Zhang, Caixia Deng, Xiupei Yang
Summary: In this study, silver-doped gold-based bimetallic nanoclusters were synthesized and demonstrated as an effective and reliable sensor system for the sensitive detection of cysteine.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Huihui Mei, Dongmei Wang, Minghui Wang, Xin Gu, Xuejun Liu, Li Yang
Summary: A novel fluorescent probe Toc-Ac based on hydroxyquinoline conjugated with a charged trimethylindolenine unit was developed, showing long wavelength emission and a large Stokes shift due to intramolecular charge transfer process. The probe exhibited highly sensitive response to Cys and was successfully applied for detecting endogenous Cys in living cells and mice.
ANALYTICAL METHODS
(2021)
Review
Chemistry, Organic
Kelton A. Schleyer, Lina Cui
Summary: Cysteine cathepsins play crucial roles in physiopathological processes and have potential as targets or biomarkers for diseases and medical conditions. Various design strategies and imaging modalities have been employed to achieve tailored selectivity for specific cathepsins.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Article
Spectroscopy
Boxin Zhou, Beibei Wang, Mengqi Bai, Mingdong Dong, Xu Tang
Summary: Cysteine plays an important role in the human body, and abnormal concentration can lead to various diseases. Detecting cysteine in vivo with high selectivity and sensitivity is therefore significant. In this study, a small organic fluorescent probe ZHJ-X was designed and synthesized based on cyanobiphenyl, which specifically recognized cysteine. The probe ZHJ-X demonstrated specific selectivity, high sensitivity, short response time, good anti-interference ability, and a low detection limit of 3.8 x 10-6 M. It was successfully applied for visualizing cysteine in living cells and holds potential for cell imaging and detection.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Multidisciplinary
Yujie Han, Junjie Shi, Songrong Li, Tingting Dan, Wenwen Yang, Mingyu Yang
Summary: This article presents a transition metal-free strategy for late-stage peptide modification, which involves site-selective functionalization under visible light to cyclize the linear aliphatic side chain at the peptide N-terminus into a proline skeleton, with excellent regio- and stereo-selectivity.
Article
Chemistry, Multidisciplinary
Yufei Di, Endong Zhang, Zhiwen Yang, Qi Shen, Xuancheng Fu, Gang Song, Chuanwei Zhu, Haotian Bai, Yiming Huang, Fengting Lv, Libing Liu, Shu Wang
Summary: In this study, an artificial enzyme, PTT-SGH, responsive to reactive oxygen species (ROS), was obtained by introducing a catalytic histidine residue to pentaerythritol tetra(3-mercaptopropionate) (PTT). The artificial enzyme formed large aggregates in cells via intracellular ROS-mediated oxidation of thiol groups, showing significantly enhanced catalytic activity in tumor cells. The artificial enzyme could trigger the cleavage of the ester bond of caged fluorophores inside living cells, enabling selective fluorescence imaging of tumor cells.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Madhav L. Ghimire, Bobby D. Cox, Cole A. Winn, Thomas W. Rockett, Nicholas P. Schifano, Hannah M. Slagle, Frank Gonzalez, Massimo F. Bertino, Gregory A. Caputo, Joseph E. Reiner
Summary: Recent research has shown that ligand-induced structure fluctuations of gold nanoclusters can be monitored using resistive-pulse nanopore sensing. The fluctuations can be used to identify the attaching peptide, either through the magnitude of current jumps or the onset of high-frequency current fluctuations. The presence of a cysteine residue in the peptide is required for the attachment process, suggesting that this method could be used for selective detection of cysteine-containing peptides. The study also demonstrates the ability to identify multiple cysteine-containing peptides in a mixture.
Article
Chemistry, Analytical
In-ho Song, Gyu Seong Yeom, Anil Kuwar, Satish Balasaheb Nimse
Summary: In this study, a benzimidazole-based compound and its derivative probe were synthesized for the highly selective detection and quantification of cysteine in human serum. The derivative probe showed high selectivity and sensitivity for cysteine and achieved lower detection limits compared to reported methods in serum samples.
Article
Chemistry, Multidisciplinary
Xu Jiang, Rui-Xue Ji, Jiang-Shan Shen
Summary: Designing an imine-based fluorescent probe capable of suppressing the tendency of intrinsic hydrolysis reaction is an attractive topic in chemo-/biosensing. In this study, a hydrophobic 1,1'-binaphthyl-2,2'-diamine was used to synthesize a probe R-1 with two imine bonds linked by two salicylaldehyde groups. The designed imine-based probe showed a high selectivity in fluorescence response by coordinating with Al3+ ions and suppressing the intrinsic hydrolysis reaction.
Article
Spectroscopy
Arul Pundi, Chi-Jung Chang, Jemkun Chen, Shih-Rong Hsieh, Ming-Ching Lee
Summary: The development of fluorescence molecules for the detection of L-tryptophan has attracted attention, and a chiral sensor SDPA was synthesized. It showed fluorescence quenching by Fe3+ and Cu2+ and could detect L-tryptophan and L-cysteine at trace levels by turn-on fluorescence.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Yonghwang Ha, Dhiraj P. Murale, Changsuk Yun, Sudesh T. Manjare, Hyungjun Kim, Juhyoun Kwak, Yoon Sup Leea, David G. Churchill
CHEMICAL COMMUNICATIONS
(2015)
Article
Chemistry, Multidisciplinary
Dhiraj P. Murale, Seong Cheol Hong, JiHyeon Yun, Chang No Yoon, Jun-Seok Lee
CHEMICAL COMMUNICATIONS
(2015)
Article
Chemistry, Multidisciplinary
Yonghwang Ha, Dhiraj P. Murale, Sudesh T. Manjare, Minseong Kim, Jeong A. Jeong, David G. Churchill
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
(2016)
Article
Biochemical Research Methods
Seong Cheol Hong, Dhiraj P. Murale, Jun-Seok Lee
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
(2016)
Review
Biochemical Research Methods
Dhiraj P. Murale, Seong Cheol Hong, Md. Mamunul Haque, Jun-Seok Lee
Article
Biochemistry & Molecular Biology
Dhiraj P. Murale, Seong Cheol Hong, Md Mamunul Haque, Jun-Seok Lee
Article
Chemistry, Multidisciplinary
Dhiraj P. Murale, Sudesh T. Manjare, Yoon-Sup Lee, David G. Churchill
CHEMICAL COMMUNICATIONS
(2014)
Article
Biochemistry & Molecular Biology
Dhiraj P. Murale, Seong Cheol Hong, Se-young Jang, Jun-Seok Lee
Review
Biochemistry & Molecular Biology
Se-Young Jang, Dhiraj P. Murale, Anne Doyoung Kim, Jun-Seok Lee
Review
Biochemistry & Molecular Biology
Md. Mamunul Haque, Dhiraj P. Murale, Yun Kyung Kim, Jun-Seok Lee
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Review
Biochemistry & Molecular Biology
Md Mamunul Haque, Dhiraj P. Murale, Jun-Seok Lee
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Chemistry, Multidisciplinary
Dhiraj P. Murale, Md Mamunul Haque, Kyung Tae Hong, Jun-Seok Lee
Summary: The schematic illustrates the use of pyridinyl-pyrazole BODIPY for lipid droplet imaging.
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
(2021)
Review
Biochemistry & Molecular Biology
Nicholas Asiimwe, Mohammad Faysal Al Mazid, Dhiraj P. Murale, Yun Kyung Kim, Jun-Seok Lee
Summary: N-terminal protein modifications play critical roles in cellular processes, and chemical probes are important tools for studying these modifications. N-terminal cysteine is a practical option for overcoming regioselectivity challenges.
Article
Chemistry, Applied
Dhiraj P. Murale, Md Mamunul Haque, Seong Cheol Hong, Se-young Jang, Jung Hoon Lee, Seo Jeong An, Jun-Seok Lee
Summary: By synthesizing five BODIPY fluorophores and investigating their photophysical and photo-crosslinking properties, pcBDaza-Mito was discovered to selectively stain mitochondria in live-cell conditions and exhibit a 2.3-fold increase in photo-crosslinking efficiency to mitochondria proteins. These results demonstrate that the bifunctional BODIPY probe has great potential for robust mitochondria imaging in live cells.
Article
Chemistry, Multidisciplinary
Seong Cheol Hong, Dhiraj P. Murale, Minju Lee, Seung Mi Lee, Joong Shin Park, Jun-Seok Lee
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2017)
