Journal
RSC ADVANCES
Volume 4, Issue 38, Pages 19970-19979Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ra02928c
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- German Federal Ministry of Education and Research (BMBF)
- Israeli Ministry of Sciences and Arts (MOST) (German-Israeli Water Technology Research Fund) [WT1103/02WA1223]
- BMBF-MOST Young Scientist Exchange Program [YSEP76]
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The atrazine-degrading bacterial strain Pseudomonas sp. ADP was immobilized by the sol-gel process within thin silica layers coated onto water-retaining carrier materials (expanded clay pellets and scoria). The performance of the obtained biohybrid material has been investigated concerning long-term activity under non-growth conditions. Experiments were run in phosphate buffer containing atrazine (20 mg l(-1)) as the sole source of carbon and nitrogen. Even after one year of consecutive batch tests, P. ADP immobilized onto expanded clay pellets showed a high atrazine degradation activity. In the course of long-term batch experiments, the average amount of removed atrazine was about 94% during each assay cycle. Staining with CTC revealed that in spite of cultivation under non-growth conditions over a period of one year, immobilized cells were still vital and showed respiratory activity.
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