Journal
RSC ADVANCES
Volume 3, Issue 44, Pages 22008-22013Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3ra43382j
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Funding
- Department of Chemical Engineering at Monash University
- Australian Research Council (ARC) [DP1094070]
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Enzyme encapsulation is an attractive method among the different immobilization strategies to improve the reusability and stability of enzymes because it can separate enzymes from a hazardous external environment. However, current encapsulation methods have limitations including enzyme leakage. In this study, a new approach based on a two-step soft templating method has been proposed to encapsulate lipase within substrate permeable mesoporous silica yolk-shell spheres. In the first step, lipase was immobilized onto epoxy functionalized silica nanospheres that serve as the core materials. The core materials were mixed with a fluorocarbon surfactant, FC4, to form a core-vesicle complex. In the second step, a mesoporous silica shell was assembled surrounding the core-vesicle complex to form the yolk-shell structure with the lipase encapsulated. The mesoporous silica shell has a pore size of 2.1 nm, which is permeable to the reactant and product while isolating the enzymes from harmful external conditions. The encapsulated lipase retained 87.5% of its activity after thermal treatment at 70 degrees C for 2 hours while the free enzyme lost 99.5% of its activity under the same treatment. Importantly, the encapsulated lipase shows significantly enhanced resistance to degradation by proteases.
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