Article
Chemistry, Physical
Wenqin Yao, Dongjian Zhu, Yun Ye, Biao Wang, Wei Xie, Aishan Ren
Summary: A novel colorimetric and ratiometric fluorescent probe 1 was designed and synthesized for the detection of Cu2+. Probe 1 exhibited maximum absorption and fluorescence emission peaks at 385 nm and 495 nm, respectively, with a large Stokes shift of 110 nm. In the presence of Cu2+, a bathochromic shift to 415 nm and 560 nm was observed in the absorption and fluorescence emission peaks, respectively, due to the Cu2+-induced hydrolysis reaction. The probe showed a significant change in the fluorescence intensity ratio (F560 nm/F495 nm), with high sensitivity, fast response time, and excellent selectivity for Cu2+ over other metal ions in aqueous solution.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Spectroscopy
Hang Su, Jingpei Wang, Xiuxiu Yue, Benhua Wang, Xiangzhi Song
Summary: A ratiometric fluorescent probe MA-N2H4 was developed for simple and convenient detection of hydrazine (N2H4) in environmental and biological systems. The probe exhibited a significant blue-shifted emission and a rapid increase in fluorescent intensity ratio after treatment with N2H4, allowing for fast detection. Additionally, the probe was successfully used for tracking N2H4 in living cells and zebrafish.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Spectroscopy
Li Liu, Yuanyuan Cui, Yixian Yang, Weiju Zhu, Cun Li, Min Fang
Summary: A novel multifunctional fluorescent probe LL2 was synthesized by condensation reactions between 3-formyl-N-butylcarbazole and 2-Hydroxy-1-naphthylhydrazone. LL2 was able to detect Cu2+ through colorimetric and ratiometric fluorescence signals. It exhibited a rapid response within 5 seconds upon adding Cu2+ and achieved stability within 30 seconds. LL2 can capture fluorescence signals in living cells and detect Cu2+ in water samples, indicating its potential for monitoring Cu2+ in the environment and living cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Applied
Yan Zhang, Haiyan Yang, Mingxin Li, Shuai Gong, Jie Song, Zhonglong Wang, Shifa Wang
Summary: A novel ratiometric fluorescent probe BIDID was designed to track exogenously and endogenously generated HClO in vitro and vivo, demonstrating high responsiveness, low detection limit, rapid response, and applicability across a wide pH range. Confocal fluorescent images showed that BIDID can serve as a reliable tool for studying HClO-related physiological and pathological processes both in vitro and in vivo.
Article
Materials Science, Multidisciplinary
Guo-Qiang Zhang, Hao Chang, Zhiyuan Gao, Yang-ping Deng, Sheng Zeng, Luqing Shang, Dan Ding, Qian Liu
Summary: In this study, a neuraminidase-responsive, near-infrared (NIR) fluorescence probe (SA-DCM) with both ratiometric and colormetric properties was reported for various applications. The ratiometric properties of SA-DCM allowed for increased anti-interference capacity, enhanced detection accuracy, and outstanding sensitivity and selectivity. A colorimetric approach was also established using SA-DCM, allowing for direct color conversion on a cotton swab to detect neuraminidase, with simplicity and practicality as the result could be directly read by the naked eye.
ACS MATERIALS LETTERS
(2023)
Article
Engineering, Environmental
Zhengjun Wu, Dan Zhang, Hailong Ma, Erfei Wang, Feiyi Wang, Jun Ren
Summary: Mitochondria play important roles in cellular processes, and their oxidative damage is a crucial factor in apoptosis, epilepsy, and other diseases. Researchers have developed a mitochondria-targeted fluorescent probe, Mito-Pa, which enables imaging of mitochondrial oxidative damage and could contribute to the diagnosis and treatment of related diseases.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Spectroscopy
Rui Guo, Qing Liu, Yonghe Tang, Shushun Cai, Xiaoya Li, Xi Gong, Yingjie Yang, Weiying Lin
Summary: Small molecule biological thiols play crucial roles in maintaining cellular activities and abnormal levels are associated with diseases. A new fluorescent probe Ty-Cys1 was designed in this study for effective monitoring of Cys changes.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Keqin Yang, Li Hou, Zhifang Li, Tianran Lin, Jianniao Tian, Shulin Zhao
Summary: A ratiometric fluorescent nanoprobe for detecting peroxynitrite was successfully constructed, showing selective accumulation in mitochondria and linear detection in the range of 0-6.0 µM. This nanoprobe enables ratiometric fluorescence imaging of endogenous peroxynitrite in cell mitochondria.
Article
Spectroscopy
Bingpeng Guo, Tianjiao Shen, Yifan Liu, Jing Jing, Changxiang Shao, Xiaoling Zhang
Summary: Esterase is primarily distributed in the endoplasmic reticulum (ER) and often overexpressed in cancer cells. To solve the limitations of existing probes, an ER-specific ratiometric fluorescent probe (ER-EST) is designed for detecting esterase in living cells. The ER-EST shows a ratiometric and red-shifted emission from 435 to 560 nm after hydrolysis by esterase, and it has a low detection limit and good biocompatibility.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Applied
Li Liu, Yixian Yang, Yuanyuan Cui, Weiju Zhu, Min Fang, Cun Li
Summary: A novel dual emissive fluorescent probe SFQ was designed and synthesized, which showed high selectivity and sensitivity towards ClO-. The probe exhibited noticeable color changes from orange to pink under natural light and from orange-red fluorescence to cyan fluorescence under a 365 nm UV lamp upon addition of ClO-. It also had a ratiometric fluorescence response to ClO- and a detection limit of 1.4 x 10-7 mol/L. The probe was successfully used to monitor ClO- in HepG2 cells and detect ClO- in actual environmental water samples.
Article
Chemistry, Applied
Xiaohang Xu, Haichang Ding, Qian Zhang, Gang Liu, Shouzhi Pu
Summary: In this study, a ratiometric fluorescent probe MYQ with an extremely large emission shift was designed for the detection of hypochlorite. The probe exhibited strong fluorescence color change in the presence of hypochlorite and showed excellent selectivity and sensitivity. The potential practicality of MYQ was demonstrated through strip test and cell imaging.
Article
Spectroscopy
Weiliang Shen, Hanhan Xu, Jing Feng, Wei Sun, Guoxing Hu, Yonghong Hu, Wenge Yang
Summary: A novel fluorescent probe TFBN has been developed based on the principle of FRET, showing specific recognition towards SO2 derivatives with advantages such as short response time, low detection limit, and good biocompatibility, and successfully used in living cells and mouse imaging.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Inorganic & Nuclear
Hong Wang, Changli Zhang, Zhiyong Jiang, Li Xu, Zhipeng Liu
Summary: In this study, phenothiazine-fused boron complexes were identified as promising scaffolds for ratiometric fluorescent hypochlorite probes. The synthesized complexes demonstrated ultrafast and ratiometric absorption/fluorescence changes for hypochlorite. Additionally, an endoplasmic reticulum-targeting probe was developed and showed excellent real-time imaging ability for both endogenous and exogenous hypochlorite in living cells.
DALTON TRANSACTIONS
(2023)
Article
Chemistry, Analytical
Lei Hu, Chengjie Sun, Renxiang Cheng, Xinhong Gao, Jiayi Zhou, Yi Wang, Ruping Jiang, Xiao Zhu, Peng Liu, Zhengquan Yan
Summary: A D-pi-A-pi-D conjugated 3,5-di-(2-hydroxyl naphthaldehyde)-iminyl triazole (HNIT) was developed for efficient monitoring of Cu2+. HNIT showed superior UV-vis and fluorescent spectral properties, and exhibited high selectivity towards Cu2+. Under optimized conditions, a fluorescent and colorimetric detection method based on HNIT achieved low detection limits and can be applied to real environmental samples.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Chunwei Yu, Mei Yang, Shuhua Cui, Yuxiang Ji, Jun Zhang
Summary: A novel ratiometric Cu2+-selective probe was constructed based on pyrene derivative, which showed selective recognition and detection of Cu2+ through a turn-on fluorescent response. The formation of a 1:1 metal-ligand complex and the binding mode were confirmed by MS spectra, Job's plot experiment, and H-1 NMR experiment. Satisfactory results were obtained from fluorescence imaging in living cells for Cu2+ detection.
