Article
Chemistry, Multidisciplinary
Vincent Clark, Kelly Waters, Ben Orsburn, Namandje N. Bumpus, Nandini Kundu, Jonathan T. Sczepanski, Partha Ray, Netzahualcoyotl Arroyo-Curras
Summary: Human cyclophilin B (CypB) is oversecreted by pancreatic cancer cells and can be used as a potential biomarker for early-stage disease diagnosis. This research used ultrasensitive electrochemical sensors to measure the hydrolytic activity of CypB, providing evidence that CypB can cleave nucleic acids. Future development of CypB bioassays will require the use of nuclease-resistant aptamer sequences.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Physiology
Rebeca Alvarino, Amparo Alfonso, Nadia Perez-Fuentes, Jesus M. Gonzalez-Jartin, Sandra Gegunde, Mercedes R. Vieytes, Luis M. Botana
Summary: Extracellular cyclophilins have been found to have various effects on endothelial function and inflammation. This study analyzed the impact of eCyps A, B, and C on endothelial cells and found that eCyps A and C increased the release of pro-inflammatory markers and activated CD147 receptor, while eCypB did not have these effects. Additionally, eCypC was found to induce endothelial dysfunction and alter the expression of other cyclophilins. This study provides new insights into the role of eCyps in vascular complications associated with diabetes.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Cell Biology
Shaoshan Mai, Iwona Inkielewicz-Stepniak
Summary: Platelets have been identified as crucial players in hemostasis, thrombosis, and cancer progression. Research has shown that platelets can promote tumorigenesis and metastasis by interacting with cancer cells in various ways. Understanding the interaction between pancreatic cancer and platelets, as well as the underlying mechanisms, may offer insights into potential diagnostic and therapeutic strategies for combating the devastating disease.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Oncology
Nianhong Chen, Qiaoqiao Zheng, Guoqing Wan, Feng Guo, Xiaobin Zeng, Ping Shi
Summary: Pancreatic cancer is aggressive with low survival rates and high risk of recurrence. Posttranslational modifications play a crucial role in the pathogenesis of pancreatic cancer. Further investigation into posttranslational modifications could be a critical field of study in relation to pancreatic cancer.
CANCER AND METASTASIS REVIEWS
(2021)
Article
Biotechnology & Applied Microbiology
Junhong Chen, Xiaoyan Zhuang, Jiyang Zheng, Ruofan Yang, Fei Wu, Aihui Zhang, Baishan Fang
Summary: Aptamers, as functional nucleic acids, offer advantages over antibodies in terms of stability and ease of modification, and an Aptamer-Based Cell-free Detection (ABCD) system combining aptamers with CRISPR-Cas12a showed promise in detecting target proteins with low abundance. This system demonstrated high specificity and signal amplification capability, paving the way for future advancements in portable detection devices.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Hauh-Jyun Candy Chen, Chau-Yi Chen, Ya-Hsuan Fang, Kai-Wei Hung, Deng-Chyang Wu
Summary: In this study, two types of Malondialdehyde (MDA)-induced modifications were identified in human hemoglobin, and it was found that these modifications were significantly higher in smokers than in non-smokers, suggesting that they could serve as biomarkers for MDA-induced protein damage.
JOURNAL OF PROTEOME RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Jennifer Behbodikhah, Saba Ahmed, Ailin Elyasi, Lora J. Kasselman, Joshua De Leon, Amy D. Glass, Allison B. Reiss
Summary: Apolipoprotein B plays a crucial role in atherogenic lipoproteins, with two major isoforms: apoB48 and apoB100. ApoB concentration serves as a direct measure of circulating atherogenic lipoproteins, independent of particle cholesterol content, making it a more accurate indicator of cardiovascular risk than total cholesterol or LDL cholesterol.
Review
Immunology
Jiaqi Kang, Jie Wu, Qinjie Liu, Xiuwen Wu, Yun Zhao, Jianan Ren
Summary: This review discusses the importance of STING in immune responses and its role in disease pathogenesis when over-activated. The focus is on post-translational modifications that regulate the activation and inhibition of STING, and their potential as targeted therapies.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Behavioral Sciences
A. Khayachi, L. Schorova, M. Alda, G. A. Rouleau, A. J. Milnerwood
Summary: The effectiveness and mode of action of lithium treatment for neurological conditions remains not fully understood, although studies have shown its impact on protein posttranslational modifications and neuronal physiology. Determining specific posttranslational modification patterns could be a promising strategy to develop disease biomarkers and predict lithium responsiveness.
NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Hua Huang, Huan Li, Ruining Pan, Sijia Wang, Xinhui Liu
Summary: The discovery of m6A has led to increasing interest in RNA epitranscriptomics. tRNAs play a crucial role in cellular function and their abnormal modifications can lead to diseases. Changes in tRNA modifications are associated with human cancers, including pancreatic cancer, suggesting potential therapeutic targets.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Biophysics
Wanming Li, Chia-Chun Wu, Shuo Wang, Linlin Zhou, Lei Qiao, Wei Ba, Furong Liu, Linan Zhan, Hang Chen, Jau-Song Yu, Jin Fang
Summary: In this study, a new metastatic biomarker W3 and its target molecule EphA2 were discovered. A cell-SELEX-based biomarker discovery strategy was proposed, which allows for a faster and more targeted discovery of biomarkers.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Nanoscience & Nanotechnology
Mei Liu, Zhifei Wang, Song Li, Yan Deng, Nongyue He
Summary: Precise diagnosis of breast cancer molecular subtypes is still a major challenge. This study identified the cell membrane protein PHB2 as a potential molecular biomarker specifically expressed in MCF-7 breast cancer cells. The findings suggest that PHB2 protein in the cell membrane may serve as a potential biomarker for accurate diagnosis and personalized therapy of the luminal A breast cancer cell subtype.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Biochemistry & Molecular Biology
Man-Yu Chu, He-Cheng Huang, En-Ming Li, Li-Yan Xu
Summary: Cyclophilin A (CypA) is a highly conserved protein that is up-regulated in various human cancers and strongly associated with malignant transformation. Surgery, chemotherapy, and radiotherapy are the main treatments for cancer.
CURRENT MEDICINAL CHEMISTRY
(2021)
Review
Medicine, Research & Experimental
Xue Han, Bo Li, Shitai Zhang
Summary: This article summarizes the molecular mechanisms of MIR503HG in disease and its potential applications in diagnosis, prognosis, and treatment. It also raises some unanswered questions in this area, providing insights for future research.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Immunology
Jinghong Yuan, Zhao Yuan, Aifang Ye, Tianlong Wu, Jingyu Jia, Jia Guo, Jian Zhang, Tao Li, Xigao Cheng
Summary: This study identified G protein subunit gamma 12 (GNG12) as a potential biomarker for osteosarcoma prognosis and highlighted its potential as an immunotherapy target. GNG12 may promote osteosarcoma through regulating the endoplasmic reticulum, and HOTTIP/miR-27a-3p may regulate GNG12 expression. The subunit suppresses adaptive immunity via inhibiting M1 and M2 macrophage infiltration in osteosarcoma.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Jayanth S. Shankara Narayanan, Partha Ray, Ibtehaj Naqvi, Rebekah White
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2018)
Article
Oncology
Jayanth S. Shankara Narayanan, Partha Ray, Tomoko Hayashi, Thomas C. Whisenant, Diego Vicente, Dennis A. Carson, Aaron M. Miller, Stephen P. Schoenberger, Rebekah R. White
CANCER IMMUNOLOGY RESEARCH
(2019)
Article
Oncology
Sudeep Banerjee, Hyunho Yoon, Mayra Yebra, Chih-Min Tang, Mara Gilardi, Jayanth S. Shankara Narayanan, Rebekah R. White, Jason K. Sicklick, Partha Ray
MOLECULAR CANCER THERAPEUTICS
(2020)
Article
Biophysics
Chih-Cheng Huang, Partha Ray, Matthew Chan, Xiahan Zhou, Drew A. Hall
BIOSENSORS & BIOELECTRONICS
(2020)
Article
Surgery
Jayanth S. Shankara Narayanan, Diego A. Vicente, Partha Ray, Louis F. Chai, Suna Erdem, Matthew J. Carr, Benedict A. Capacio, Bryan F. Cox, David B. Jaroch, Steven C. Katz, Rebekah R. White
Article
Biophysics
Naveen K. Singh, Partha Ray, Aaron F. Carlin, Celestine Magallanes, Sydney C. Morgan, Louise C. Laurent, Eliah S. Aronoff-Spencer, Drew A. Hall
Summary: The study introduces an aptamer-based SARS-CoV-2 salivary antigen assay that utilizes low-cost reagents and an off-the-shelf glucometer. This test is highly scalable, provides rapid results within 1 hour, and can accurately detect viral loads in infected specimens while distinguishing them from uninfected controls.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Saeromi Chung, Jason K. Sicklick, Partha Ray, Drew A. Hall
Summary: An electrochemical sensor based on a conformation-changing aptamer has been developed to detect the cancer biomarker soluble KIT in human serum. The sensor shows high specificity and a wide dynamic range, successfully detecting KIT in different liquids.
Article
Multidisciplinary Sciences
Naveen K. Singh, Partha Ray, Aaron F. Carlin, Sydney C. Morgan, Celestine Magallanes, Louise C. Laurent, Eliah S. Aronoff-Spencer, Drew A. Hall
Summary: The study presents a method for detecting SARS-CoV-2 antigen using an aptamer-based assay, which can be conducted at home or in clinics without expensive instruments or professional training. The optimization of parameters and validation of assay performance provide a foundation for developing glucometer-based assays for diagnosing other diseases.
Article
Biochemistry & Molecular Biology
Leonardo Antonio Fernandes, Anderson Albino Gomes, Beatriz Gomes Guimaraes, Maria de Lourdes Borba Magalhaes, Partha Ray, Gustavo Felippe da Silva
Summary: This study successfully designed ACE2 mimetic ligands with high affinity using protein engineering techniques to disrupt the protein-protein interaction between the SARS-CoV-2 virus and host cells. The research provides a promising scaffold for the development of high-affinity ACE2 mimetics.
Article
Chemistry, Multidisciplinary
Vincent Clark, Kelly Waters, Ben Orsburn, Namandje N. Bumpus, Nandini Kundu, Jonathan T. Sczepanski, Partha Ray, Netzahualcoyotl Arroyo-Curras
Summary: Human cyclophilin B (CypB) is oversecreted by pancreatic cancer cells and can be used as a potential biomarker for early-stage disease diagnosis. This research used ultrasensitive electrochemical sensors to measure the hydrolytic activity of CypB, providing evidence that CypB can cleave nucleic acids. Future development of CypB bioassays will require the use of nuclease-resistant aptamer sequences.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Virology
Bruna Andersen Pereira de Jesus, Anderson Albino Gomes, Alex E. E. Clark, Tayse Andrade Rodrigues, Melissa Ledgerwood-Lee, Westley Van Zant, Howard Brickner, Meiqiao Wang, David L. L. Blum, Maria B. B. Cassera, Aaron F. F. Carlin, Eliah S. S. Aronoff-Spencer, Gustavo Felippe da Silva, Maria de Lourdes Borba Magalhaes, Partha Ray
Summary: The use of a engineered mini-protein, p-deface2 mut, in an ELISA assay can effectively detect neutralizing antibodies against SARS-CoV-2 in patient sera. This cost-effective method could be particularly important in low-income countries where economical diagnostic testing is crucial.
Article
Chemistry, Multidisciplinary
Sanahan Vijayakumar, Seyedmehdi H. Nasr, Jacob E. Davis, Edward Wang, Jonathan M. Zuidema, Yi-Sheng Lu, Yu-Hwa Lo, Jason K. Sicklick, Michael J. Sailor, Partha Ray
Summary: Evaluation of Gastrointestinal Stromal Tumors (GIST) can be challenging due to limitations of current imaging modalities. A novel nanoparticle platform that utilizes a DNA aptamer targeting KIT, decorated onto a fluorescently labeled porous silicon nanoparticle, shows promise for high-resolution imaging of GIST with minimal cellular toxicity. In vitro and in vivo analyses demonstrate specific protein targeting and extensive nanoparticle-to-tumor signal co-localization, providing an effective platform for imaging KIT-expressing cancers or targeted drug delivery.
Article
Oncology
Jayanth Surya Narayanan Shankara Narayanan, Katie Frizzi, Suna Erdem, Partha Ray, David Jaroch, Bryan Cox, Steven Katz, Diego Vicente, Rebekah White
Summary: This study explores the possibility of reducing peripheral neuropathy in the treatment of pancreatic ductal adenocarcinoma (PDAC) by regional pressurized delivery of oxaliplatin. The results showed that delivering oxaliplatin through the portal vein using a pressure-enabled system significantly reduced tumor burden and peripheral neuropathy in mice. Therefore, regional delivery of highly cytotoxic agents can lower the therapeutic dose and minimize toxic side effects.
Meeting Abstract
Oncology
Jayanth S. Shankara Narayanan, Aaron Miller, Tomoko Hayashi, Partha Ray, Stephen Schoenberger, Dennis Carson, Rebekah White
CANCER IMMUNOLOGY RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Partha Ray, Rebekah R. White
JOURNAL OF NUCLEIC ACIDS
(2017)