Article
Agriculture, Multidisciplinary
Qi Zhang, Chang Yuan, Feng Wang, Sha Xu, Youran Li, Guiyang Shi, Zhongyang Ding
Summary: This study demonstrated that the combination of ssPOXA3a/b with POXA3 enhances the activity, thermostability, and pH stability of POXA3, while having a negative feedback effect on lacc6 transcription. Furthermore, overexpression of ssPOXA3a/b led to small fungal pellets, thin mycelial walls, and facilitated laccase secretion, providing insights for the development of high-yield laccase.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Microbiology
Qi Zhang, Liting Zhao, Mengye Shen, Jingyun Liu, Youran Li, Sha Xu, Lei Chen, Guiyang Shi, Zhongyang Ding
Summary: In this study, a polyethylene glycol-mediated transformation system was established in Pleurotus eryngii var. ferulae, resulting in an improved transformation efficiency. The study also identified and evaluated three endogenous promoters, enabling the establishment of a controllable overexpression system for heterologous gene expression.
Article
Food Science & Technology
Shuang Lv, Xiaolin Zhu, Zhenbin Liu, Liangbin Hu, Dan Xu, Bimal Chitrakar, Haizhen Mo, Hongbo Li
Summary: This study evaluated the feasibility of preparing papery food using Pleurotus eryngii as a raw material through the papermaking process. The results showed that PSP and PMP were more popular among consumers in terms of formability, mouth feel, and overall palatability, with PSP also exhibiting better moisture resistance and thermal decomposition performance.
Article
Agronomy
Junmin Jia, Fei Hu, Xubo Zhang, Zongyou Ben, Yifan Wang, Kunjie Chen
Summary: This study proposes an Attention-Based CNN model for automatic weight detection of Pleurotus eryngii using machine vision technology and deep learning algorithms. Compared with traditional feature screening tasks, the model shows better performance.
Article
Biochemistry & Molecular Biology
Pengfei Yuan, Litong Liu, Adila Aipire, Yanan Zhao, Shanshan Cai, Linjia Wu, Xiaofei Yang, Alimu Aimaier, Jun Lu, Jinyao Li
Summary: It has been found that the combination of gold nanoparticles and Pleurotus ferulae polysaccharide (PFPS) can enhance the immunostimulatory activity of PFPS in the maturation and function of dendritic cells (DCs), as well as improve DC migration and murine immunity, especially in type 1 T-helper cell responses. Additionally, PFPS-Au NPs have a longer half-life in the blood and increased distribution in the spleen compared to PFPS, indicating improved bioavailability in vivo. Therefore, this study paves the way for the application of PFPS-Au NPs in clinical trials.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Food Science & Technology
Yuxi Guo, Xuefeng Chen, Pin Gong, Ruotong Wang, Zhuoya Qi, Zhenfang Deng, Aoyang Han, Hui Long, Jiating Wang, Wenbo Yao, Wenjuan Yang, Jing Wang, Nan Li
Summary: This paper reviews the postharvest preservation techniques for the king oyster mushroom, including physical and chemical methods, and discusses the storage effects of different preservation methods. It provides important research directions for the processing and product development of this mushroom.
Article
Genetics & Heredity
Dou Ye, Fang Du, Yajie Zou, Qingxiu Hu
Summary: Comparative transcriptomics analysis between mature mycelia and primordium of Pleurotus eryngii revealed the involvement of differentially expressed genes in processes such as cell wall degradation, carbohydrate hydrolysis, light perception, and cAMP signal transduction during primordium formation. These findings provide insights into the molecular processes underlying primordium formation, which can potentially improve cultivation and quality control of P. eryngii.
Article
Chemistry, Applied
Bahareh Arabpoor, Shima Yousefi, Weria Weisany, Mehran Ghasemlou
Summary: This study aimed to fabricate Eryngium campestre essential oil encapsulated chitosan nanoparticles through an ion gelation method. Coating sweet cherries with the encapsulated ECEO helped extend shelf-life, reduce microbial growth, and maintain certain physicochemical characteristics of the fruits.
FOOD HYDROCOLLOIDS
(2021)
Article
Food Science & Technology
Dimitra Tagkouli, Georgios Bekiaris, Stella Pantazi, Maria Eleni Anastasopoulou, Georgios Koutrotsios, Athanasios Mallouchos, Georgios I. Zervakis, Nick Kalogeropoulos
Summary: The study found that Pleurotus ostreatus had higher concentrations but fewer volatile compounds in fresh samples compared to P. eryngii. Roasting resulted in elimination of some volatiles and formation of new compounds. Principal component analysis successfully differentiated among mushroom species and strains, but not among different cultivation substrates.
Article
Immunology
Xue Wang, Yunhe Qu, Yuan Wang, Xiang Wang, Jialei Xu, Hailing Zhao, Donglin Zheng, Lin Sun, Guihua Tai, Yifa Zhou, Hairong Cheng
Summary: The polysaccharides from Pleurotus eryngii, specifically APEP-A-b, have been found to have positive effects on immunity and gut microbiota. APEP-A-b can increase lymphocyte proliferation, NK cell activity, and phagocytic capacity of peritoneal cavity phagocytes. It also increases the proportion of CD4(+) and CD8(+) T cells in the lamina propria and induces changes in the gut microbiota composition. These findings are important for the development of polysaccharides derived from Pleurotus eryngii.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tania Petraglia, Tiziana Latronico, Angela Fanigliulo, Aniello Crescenzi, Grazia Maria Liuzzi, Rocco Rossano
Summary: This study evaluated the antioxidant and neuroprotective activity of an enriched polysaccharide fraction (EPF) obtained from the fruiting body of cultivated P. eryngii. The EPF showed high content of (1-3; 1-6)-beta-D-glucans and exhibited significant scavenging activities against DPPH, superoxide, hydroxyl, and nitric oxide radicals. It also demonstrated biocompatibility and reduced oxidative stress in DI-TNC1 cells. These findings suggest that polysaccharides extracted from P. eryngii could be used as functional food to enhance antioxidant defenses.
Article
Engineering, Chemical
Anqi He, Juan Xu, Qiuhui Hu, Liyan Zhao, Gaoxing Ma, Lei Zhong, Rui Liu
Summary: In this study, we improved the 3D printing performance of Pleurotus eryngii by adding six gums. Among them, locust bean gum showed superior appearance and printing accuracy, with the best 3D printing performance. It increased the hardness, viscosity, and storage modulus of Pleurotus eryngii ink by 42.7%, 33.1%, and 105.2% respectively, and presented a dense and continuously uniform microstructure.
JOURNAL OF FOOD ENGINEERING
(2023)
Article
Chemistry, Applied
Gaoxing Ma, Qian Xu, Hengjun Du, Benard Muinde Kimatu, Anxiang Su, Wenjian Yang, Qiuhui Hu, Hang Xiao
Summary: Pleurotus eryngii polysaccharide (PEP) remains intact during digestion but degrades during fermentation, producing beneficial short-chain fatty acids (SCFAs). It can regulate the composition of gut microbiota, increasing the relative abundance of Firmicutes, thus promoting intestinal health.
Article
Biochemistry & Molecular Biology
Lei Chen, Ai Ren, Yushi Wang, Yunhe Qu, Hesong Gong, Kevin H. Mayo, Yifa Zhou, Hairong Cheng
Summary: This study demonstrates that WPEP-N-b, a heterogalactan from Pleurotus eryngii, enhances immunity in immunosuppressed mice. WPEP-N-b improves immune organ index, peripheral blood immune cell content, splenocyte proliferation, NK cell activity, and T lymphocyte subpopulations. Additionally, WPEP-N-b restores cytokine levels and macrophage activity, and its mechanisms involve Toll-like receptor and signaling pathways.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Chen Zhang, Xinling Song, Weijun Cui, Qihang Yang
Summary: The study found that PERP extracted from Pleurotus eryngii residues may serve as a potential anti-aging agent, positively impacting the brain, liver, kidneys, and skin. This polysaccharide, comprised of multiple monosaccharides, with a moderate molecular weight and spherical structure, showed promising effects in animal experiments.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Marzia Beccaccioli, Manuel Salustri, Valeria Scala, Matteo Ludovici, Andrea Cacciotti, Simone D'Angeli, Daren W. Brown, Massimo Reverberi
Summary: This study investigated the impact of fumonisins produced by Fusarium verticillioides on maize lipid metabolism, causing the fungal growth to switch from a relatively harmless endophyte to a destructive necrotroph.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jelena Loncar, Barbara Bellich, Alessia Parroni, Massimo Reverberi, Roberto Rizzo, Slaven Zjalic, Paola Cescutti
Summary: The study evaluated oligosaccharides extracted from Trametes versicolor for controlling two mycotoxins, with heptasaccharides showing up to 90% inhibitory activity. These results suggest that these oligosaccharides could be natural alternatives for controlling mycotoxins.
Review
Nutrition & Dietetics
Rita Businaro, David Vauzour, Jerome Sarris, Gerald Munch, Erika Gyengesi, Laura Brogelli, Pedro Zuzarte
Summary: Nutrition plays a crucial role in neurodegenerative mental disorders, and supplementation of essential nutrients can help reduce inflammation and lower risk. Dietary supplementation has shown effectiveness in treating mild cognitive impairment and improving depression.
FRONTIERS IN NUTRITION
(2021)
Article
Green & Sustainable Science & Technology
Paola De Santis, Dejene K. Mengistu, Yosef Gebrehawaryat Kidane, Rose Nankya, Barbara De Santis, Gabriele Moracci, Francesca Debegnach, Riccardo Marsiglia, Massimo Reverberi
Summary: This pilot study assessed the contamination level of mycotoxins in traditional varieties of Ethiopian durum wheat and Ugandan groundnuts, finding no contamination in wheat samples and negligible levels in groundnut samples. Further studies are needed to determine the role of non-intensive production systems and varieties in preventing and reducing mycotoxin contamination in crops.
Article
Entomology
Valentina Mastrantonio, Daniele Porretta, Valentina Lucchesi, Nurper Guz, Naciye Sena Cagatay, Romeo Bellini, John Vontas, Sandra Urbanelli
Summary: The application of insecticides can lead to the evolution of resistance in insect pests through the survival of individuals carrying resistant mutations. The study used a phylogenetic approach to investigate the origin of three mutations conferring resistance to diflubenzuron insecticide, finding evidence for multiple origins of the mutations. This research contributes to informing resistance management and understanding the origin of adaptive traits.
Review
Biochemistry & Molecular Biology
Federica Armeli, Alessio Bonucci, Elisa Maggi, Alessandro Pinto, Rita Businaro
Summary: The Mediterranean diet has broad-spectrum neuroprotective activity, potentially counteracting neurodegeneration by modulating the endocannabinoid system. The co-evolution of the human endocannabinoid system and dietary habits in the Mediterranean lifestyle may play a key role in pro-homeostatic activity.
Article
Agronomy
Daniele Porretta, Valentina Mastrantonio, Valentina Lucchesi, Romeo Bellini, John Vontas, Sandra Urbanelli
Summary: The study investigates the relative contribution of agricultural and public-health insecticide applications in selecting for insecticide resistance in mosquito populations. The findings suggest a combined role of agricultural and public-health use of insecticides in vector resistance across time and call for increased communication between agriculture and health practitioners for integrated resistance management.
PEST MANAGEMENT SCIENCE
(2022)
Article
Microbiology
Marzia Beccaccioli, Alessandro Grottoli, Luca Scarnati, Luigi Faino, Massimo Reverberi
Summary: The genome assembly of an Italian strain of Biscogniauxia mediterranea obtained using hybrid sequencing technologies has been reported in this study, shedding light on the pathogen's sensitivity to biotic and abiotic factors such as climate change.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Green & Sustainable Science & Technology
Simona Consoli, Cinzia Caggia, Nunziatina Russo, Cinzia Lucia Randazzo, Alberto Continella, Giulia Modica, Santa Olga Cacciola, Luigi Faino, Massimo Reverberi, Andrea Baglieri, Ivana Puglisi, Mirco Milani, Giuseppe Longo Minnolo, Salvatore Barbagallo
Summary: The study investigated the use of citrus waste as organic fertilizer and its effects on soil microbiota, physico-chemical and hydraulic characteristics. The study also identified the biotic components of the waste and its impact on nutritional status, chlorophyll content, fruit set and production of Tarocco orange trees. The results confirm that using citrus waste as a biofertilizer offers a great opportunity for sustainable sweet orange production.
Article
Entomology
Valentina Lucchesi, Lorenzo Grimaldi, Valentina Mastrantonio, Daniele Porretta, Letizia Di Bella, Tania Ruspandini, Martino Luigi Di Salvo, John Vontas, Romeo Bellini, Agata Negri, Sara Epis, Silvia Caccia, Claudio Bandi, Sandra Urbanelli
Summary: Insect pests are a global problem and controlling them is crucial for human well-being. Insecticides, specifically chitin-synthesis inhibitors (CSIs), are commonly used to control these pests, but resistance is developing in many species. This study focused on the molecular and phenotypic characteristics of the homozygous I1043M strain in Culex pipiens mosquitoes, which showed a high level of resistance to the CSI diflubenzuron. The researchers found that resistant mosquitoes had cuticle modifications and over-expression of the chs1 gene, which likely contribute to resistance. These findings are valuable for understanding CSI resistance in mosquitoes and other arthropod pests.
Article
Microbiology
Anastasia Orekhova, Marta De Angelis, Andrea Cacciotti, Massimo Reverberi, Dante Rotili, Alessandra Giorgi, Virginia Protto, Graziana Bonincontro, Francesco Fiorentino, Victor Zgoda, Antonello Mai, Anna Teresa Palamara, Giovanna Simonetti
Summary: In this study, the impact of the BET inhibitor JQ1 on the extracellular proteins and virulence of A. fumigatus was evaluated. The results showed that JQ1 could significantly modulate the expression of extracellular proteins and attenuate the virulence of A. fumigatus. Furthermore, JQ1 was found to inhibit the expression of intracellular laccase Abr2 in A. fumigatus.
Article
Multidisciplinary Sciences
Simone Pitton, Agata Negri, Giulia Pezzali, Marco Piazzoni, Silvia Locarno, Paolo Gabrieli, Roberto Quadri, Valentina Mastrantonio, Sandra Urbanelli, Daniele Porretta, Claudio Bandi, Sara Epis, Silvia Caccia
Summary: Adult mosquito females transmit zoonotic pathogens through their bites, making control measures crucial. This study characterized MosChito rafts, a floating tool containing a bioinsecticide, which effectively attracted and killed larvae of the Asian tiger mosquito. The Bti-based formulate in the rafts remained active for over a month, proving the effectiveness of this eco-friendly solution for larval control.
SCIENTIFIC REPORTS
(2023)
Article
Food Science & Technology
Jelena Loncar, Barbara Bellich, Paola Cescutti, Alice Motola, Marzia Beccaccioli, Slaven Zjalic, Massimo Reverberi
Summary: Aflatoxin B-1 (AFB(1)) and ochratoxin A (OTA) are two important mycotoxins. Extracts from wood-decaying mushrooms can inhibit the synthesis of AFB(1) and OTA. This study screened 42 isolates of ligninolytic mushrooms and found that four isolates can inhibit OTA synthesis, and 11 isolates can inhibit AFB(1) synthesis by more than 50%. Two strains, Trametes versicolor strain TV117 and Schizophyllum commune strain S.C. Ailanto, can significantly inhibit the synthesis of both mycotoxins (>90%). The polysaccharides of S. commune may be a useful component in controlling mycotoxin synthesis.
Article
Microbiology
Giuliano Manetti, Angela Brunetti, Valentina Lumia, Lorenzo Sciarroni, Paolo Marangi, Nicola Cristella, Francesco Faggioli, Massimo Reverberi, Marco Scortichini, Massimo Pilotti
Summary: For the past decade, olive groves in Apulia, Southern Italy, have been destroyed by Olive Quick Decline Syndrome (OQDS), caused by Xylella fastidiosa subsp. pauca (Xfp). Recently, a new wilting syndrome affecting olive trees in the area has been identified as Branch and Twig Dieback (BTD), associated with the fungus Neofusicoccum mediterraneum. This study reports the first occurrence of Neofusicoccum stellenboschiana in olive trees in the northern hemisphere and highlights the high virulence of both N. stellenboschiana and N. mediterraneum in causing bark canker and wood discoloration in olive trees.
Review
Dentistry, Oral Surgery & Medicine
Massimo Fusconi, Francesca Candelori, Lior Weiss, Annachiara Riccio, Roberta Priori, Rita Businaro, Linda Mastromanno, Isotta Musy, Marco de Vincentiis, Antonio Greco
Summary: This study aims to investigate the qualitative and quantitative defects of mucins in the saliva of patients with pSS. The results suggest that pathological alterations in the chemical-physical properties of mucins are the main cause of clinical characteristics in pSS patients.
MEDICINA ORAL PATOLOGIA ORAL Y CIRUGIA BUCAL
(2021)