Article
Genetics & Heredity
Zijuan Xin, Wei Zhang, Shangjin Gong, Junwei Zhu, Yanming Li, Zhaojun Zhang, Xiangdong Fang
Summary: This study established a RBC regeneration system using iPSCs and used a single-cell transcriptome platform to map cell lineage and differentiation trajectory on day 14. The differentiation of iPSCs during embryoid body culture was found to be unsynchronized, with cells mainly consisting of mesodermal and various blood cells resembling yolk sac hematopoiesis. The study identified different stages of erythroid cells and regulatory transcription factors specific to each stage, providing theoretical guidance for optimizing iPSC-derived erythroid differentiation system.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2021)
Article
Cell & Tissue Engineering
Dachuan Zhang, Xin Gao, Huihui Li, Daniel K. Borger, Qiaozhi Wei, Eva Yang, Chunliang Xu, Sandra Pinho, Paul S. Frenette
Summary: This study reveals that the microbiota regulates hematopoietic stem cells (HSCs) self-renewal and differentiation by modulating local iron availability in the bone marrow. Microbiota depletion enhances HSC self-renewal but compromises differentiation under stress conditions. The interplay between the microbiota, macrophages, and iron is essential for regulating critical HSC fate decisions.
Article
Biology
Virginia Folgado-Marco, Kristina Ames, Jacky Chuen, Kira Gritsman, Nicholas E. Baker
Summary: A conditional knockout mouse was generated by partially deleting Rps12. Mice inheriting the Rps12(KO/+) genotype exhibited growth and morphological defects, pancytopenia, and impaired erythropoiesis. There was a significant reduction in hematopoietic stem cells and progenitors in the bone marrow of Rps12(KO/+) mice.
Letter
Oncology
Lulu Liu, Yanjia Fang, Xiaodan Ding, Weihua Zhou, Remi Terranova, Yan Zhang, He Wang
Summary: By using a hematopoietic-specific Wdr5 knockout mouse model, we found that the loss of Wdr5 leads to embryonic lethality and impairs the function of hematopoietic stem and progenitor cells. Additionally, it may alter the immunophenotype of these cells by decreasing c-Kit expression. Overall, we identified the crucial role of Wdr5 in fetal hematopoiesis and erythropoiesis as novel findings.
EXPERIMENTAL HEMATOLOGY & ONCOLOGY
(2023)
Article
Engineering, Biomedical
Gyuhyung Jin, Martha E. Floy, Aaron D. Simmons, Madeline M. Arthur, Sean P. Palecek
Summary: This study introduces a simple and versatile method to spatially pattern differentiation of human pluripotent stem cells (hPSCs) in 2-dimensional culture via localized morphogen adsorption, allowing for induction of different cell types such as mesendoderm, endoderm, and cardiomyocytes. This approach provides a platform for controlled and systematic study of early pattern formation and can be used to generate 2D patterned hPSC-derived tissue structures for modeling disease and drug interactions.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Kushagra Pandey, Hamim Zafar
Summary: MARGARET is a method for inferring single-cell trajectories and fate mapping, which can reconstruct complex cell-state manifolds and quantify fate plasticity. Evaluation on multiple datasets from different domains demonstrates that MARGARET outperforms other methods and has been successfully applied to study hematopoiesis, embryoid body differentiation, and colon differentiation.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Lu Liu, Juanyan Wu, Shuanghu Wang, Liu Kun, Junbin Gao, Bin Chen, Yicheng Ye, Fei Wang, Fei Tong, Jiamiao Jiang, Juanfeng Ou, Daniela A. Wilson, Yingfeng Tu, Fei Peng
Summary: The study presents a minimally invasive micromotor platform for precise navigation and targeting of neural stem cells, which can be directed to differentiate into specific neuron types by tuning ultrasound intensity. This platform has the potential to serve as a controllable wireless tool for bioelectronics and neuronal regenerative therapy.
Review
Cell Biology
Sydney Treichel, Marie-Dominique Filippi
Summary: Hematopoietic stem cells have the ability to self-renew or differentiate into various blood cell lineages. The cell fate decisions of self-renewal or differentiation must be tightly controlled to prevent diseases like bone marrow failure or leukemia. Studies have shown that the cell cycle length plays a crucial role in hematopoietic stem cell fate. Cell cycle kinetics, divisional history, asymmetric cell divisions, metabolic and organelle activity all have an impact on hematopoietic stem cell fate decisions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Marika Sjoqvist, Daniel Antfolk, Freddy Suarez-Rodriguez, Cecilia Sahlgren
Summary: Intermediate filaments have emerged as important regulators of cellular signaling events, beyond providing structural support, by regulating cell differentiation and influencing cell fate decisions in conjunction with stem cell signaling pathways. Lack of IFs causes perturbed stem cell differentiation, and deregulation in these signaling pathways can result in various diseases. Studying the interaction and regulation of IFs with cell signaling helps expand our understanding of IF-linked diseases.
Article
Engineering, Biomedical
Yanqun Li, Jianhui Yue, Yuan Liu, Jun Wu, Min Guan, Di Chen, Haobo Pan, Xiaoli Zhao, William W. Lu
Summary: The study revealed that strontium promotes osteogenic differentiation of mesenchymal stem cells and balances stemness maintenance and osteogenic differentiation through asymmetric cell division. Strontium activates noncanonical Wnt signaling to regulate cell division and enhance osteogenic differentiation.
ACTA BIOMATERIALIA
(2021)
Review
Cell Biology
Yuanjie Ding, Yuanchun Yao, Xingmu Gong, Qi Zhuo, Jinhua Chen, Miao Tian, Maryam Farzaneh
Summary: JMJD3 is a key histone demethylase that regulates H3K27me3 and plays a crucial role in stem cell fate determination during the process of cell reprogramming.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Cell Biology
Kulbhushan Sharma, Nagham T. Asp, Sean P. Harrison, Richard Siller, Saphira F. Baumgarten, Swapnil Gupta, Maria E. Chollet, Elisabeth Andersen, Gareth J. Sullivan, Anne Simonsen
Summary: This study investigates the role of autophagy in early events of lineage commitment using human embryonic and induced pluripotent stem cells. They found that modulation of autophagy affects SOX2 levels and lineage commitment, with autophagy promoting mesendoderm formation and inhibiting neuroectoderm formation. The results suggest that autophagy-mediated SOX2 turnover is a key factor in determining lineage commitment.
Article
Biochemical Research Methods
Marius Lange, Volker Bergen, Michal Klein, Manu Setty, Bernhard Reuter, Mostafa Bakhti, Heiko Lickert, Meshal Ansari, Janine Schniering, Herbert B. Schiller, Dana Pe'er, Fabian J. Theis
Summary: Computational trajectory inference is a method to reconstruct cell state dynamics from single-cell RNA sequencing experiments, but it requires prior knowledge of the direction of biological processes. CellRank is a new approach that allows single-cell fate mapping in scenarios where the direction is unknown, such as regeneration, reprogramming, and disease.
Review
Biochemistry & Molecular Biology
Pascal Vong, Hakim Ouled-Haddou, Loic Garcon
Summary: Numerous studies have emphasized the importance of post-translational modifications in regulating cellular processes such as cell proliferation, differentiation, and death. Acetylation, a type of modification, alters the properties and activity of proteins and plays a role in their stability, localization, and interaction with other proteins. Histone deacetylases (HDACs) are responsible for the deacetylation of many proteins, both functional and structural, in the nucleus and cytoplasm. They are involved in crucial cellular processes, including hematopoiesis, where they finely regulate the differentiation of stem cells and progenitors. Understanding the role of HDACs in hematopoiesis is important, particularly considering their potential as targets for cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Asahi Nakamuta, Kana Yoshido, Honda Naoki
Summary: This study developed a mathematical model called the hierarchical neutral competition (hNC) model, which integrates the characteristics of both the classical model and the recent model. The model was validated using experimental data and it predicts the spontaneous generation of clonal bursts in primate hematopoiesis. The findings have significant implications for understanding the maintenance and differentiation of stem cells in tissues.
COMMUNICATIONS BIOLOGY
(2022)