Article
Reproductive Biology
Ting Yang, Xi Yuan, Qingsong Xue, Le Sun, Tairan Xu, Yuan Chen, Deshun Shi, Xiangping Li
Summary: This study used Smart-seq2 technique to investigate the molecular mechanism of symmetric and asymmetric cleavage in early cleavage embryos. The results showed that asymmetric cleavage is associated with altered gene expression, and genes involved in mitochondrial function and cytoskeletal structure, such as Atg4c, Sesn2, Stk11ip, Slc25a6, Cep19 and Cep55, may be the key candidate genes determining the zygote cleavage pattern.
Article
Biochemistry & Molecular Biology
Xiaorong Huang, Meng-Xiang Sun
Summary: This study reveals the essential role of H3K27me3 in vegetative cell fate commitment and germline cell fate initiation in male Arabidopsis gametophytes. H3K27me3 erasure is necessary for triggering gamete cell fate and leads to extensive cell identity transition. The findings provide experimental confirmation for the epigenetic regulation of MG cell/VC fate and support two previous hypotheses regarding germline cell fate determination.
Article
Biochemical Research Methods
Peter Uhl, John Lowengrub, Natalia Komarova, Dominik Wodarz
Summary: This study demonstrates that the negative feedback loop from differentiated cells onto stem cell division patterns loses the ability to maintain tissue homeostasis in spatially explicit computational models. However, negative feedback on stem cell division can lead to the spatial organization of stem and differentiated cells into distinct islands. Further research shows that tissue homeostasis can be achieved by combining spatially restricted negative feedback on self-renewal with other experimentally documented spatial control mechanisms.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Anna Kosenko, Shamay Jacoby, Tomer-Meir Salame, Maya Ross, Itamar Barash
Summary: This study suggests that intramammary rapamycin administration can effectively reduce mTOR activity in bovine mammary epithelial cells and induce stem cell self-renewal. This has potential positive implications for mammary development and milk production in mature cows.
Article
Biochemistry & Molecular Biology
Sandra Manzanero-Ortiz, Ana de Torres-Jurado, Rubi Hernandez-Rojas, Ana Carmena
Summary: This study demonstrated the connection between compromised asymmetric cell division and tumorigenesis using Drosophila larval brain neural stem cells as a model system. Through a pilot RNAi screen in a sensitized genetic background, 15 potential novel ACD regulators/tumor suppressor genes were identified.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Green & Sustainable Science & Technology
Kwadwo Asante
Summary: This study uses person-organisation fit and norm activation theory to develop conceptual models that clarify the non-homogenous effect of green leadership on employee pro-environmental behavior in the hospitality industry. The study finds that value congruence and moral consciousness mediate the relationship between green leadership and employee pro-environmental behavior. The study emphasizes the importance of moral consciousness in stimulating pro-environmental behavior among employees in the hospitality sector.
JOURNAL OF SUSTAINABLE TOURISM
(2023)
Article
Biochemistry & Molecular Biology
Eriko Suzuki, Naoki Masaka, Tatsuhiro Urabe, Madoka Sasaki, Keiji Hasumi
Summary: This study reveals that the direct interaction between cancer stem cells (CSCs) and non-CSCs inhibits the division of CSCs, providing insights into the regulatory mechanism of non-CSCs on CSC proliferation in the tumor microenvironment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Nutrition & Dietetics
Shaima Sirajudeen, Iltaf Shah, Sherif M. Karam, Asma Al Menhali
Summary: Vitamin D deficiency can result from insufficient light exposure or intake. This study investigated the biological effects of vitamin D deficiency on mouse gastric glands and found that the effects were influenced by the severity of deficiency. Unique gastric phenotypes were observed in mice with light-induced vitamin D deficiency compared to diet-induced deficiency.
Article
Biochemistry & Molecular Biology
Shaima Sirajudeen, Iltaf Shah, Mohammed Akli Ayoub, Sherif M. Karam, Asma Al Menhali
Summary: This study aims to understand how gastric homeostasis is affected by vitamin D deficiency caused by prolonged exposure to darkness alone or combined with a vitamin D deficient diet. The research found that the membrane vitamin D receptor is widely expressed in the stomach, and vitamin D deficiency can lead to mucous cell expansion, increased apoptotic cells, decreased cell proliferation, and decreased acid secretion in the stomach.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Wen-Yang Hu, Dan-Ping Hu, Lishi Xie, Larisa Nonn, Ranli Lu, Michael Abern, Toshihiro Shioda, Gail S. Prins
Summary: This study characterized the epithelial cell lineage hierarchy in prostaspheres using single-cell RNA-seq analysis and keratin profiling. It identified distinct clusters of stem cells and progenitor cells based on marker expression and gene enrichment pathways. Understanding the keratin profiles in human prostate stem cell lineage hierarchy and cancer stem-like cells may aid in the identification and targeted therapy of prostate cancer stem-like cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Hanbing Song, Hannah N. W. Weinstein, Paul Allegakoen, Marc H. Wadsworth, Jamie Xie, Heiko Yang, Ethan A. Castro, Kevin L. Lu, Bradley A. Stohr, Felix Y. Feng, Peter R. Carroll, Bruce Wang, Matthew R. Cooperberg, Alex K. Shalek, Franklin W. Huang
Summary: The study characterized the microenvironment response to prostate cancer using single-cell RNA-seq and organoids, identifying tumor-associated epithelial cell states and club cells.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Sang-Woo Lee, Yoshihiro Morishita
Summary: Recent advancements in technology have allowed for direct observations of the formation and elimination of abnormal cell populations. Cell competition research now incorporates cell mechanics, with abnormal cells being mechanically eliminated in epithelial tissues through cell contraction. Mathematical analyses have revealed critical cell density types associated with mechanical elimination of abnormal cell clusters.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Teeraphan Laomettachit, Pavel Kraikivski, John J. Tyson
Summary: The cell division cycle is regulated by a complex network of interacting genes and proteins. In this study, a continuous-time stochastic model using Boolean variables and a continuous variable was developed to simulate the cell cycle progression of budding yeast cells. The model accurately captures the growth and division of yeast cells, and reveals that the cell cycle is robust to random flips of Boolean variables, but some perturbations can induce lethal mistakes in cell cycle progression.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Kimberley C. Clark, Elizabeth V. Nguyen, Birunthi Niranjan, Yunjian Wu, Terry C. C. Lim Kam Sian, Lisa G. Horvath, Renea A. Taylor, Roger J. Daly
Summary: Prostate cancer is a common cancer in men and a major cause of cancer-related deaths. In this study, we investigated the cell-type-specific signaling networks initiated by intercellular communication between prostate epithelial cells and patient-derived cancer-associated fibroblasts (CAFs) when grown together. Significant differences in proteomic profiles were identified, and the upregulation of transglutaminase-2 (TGM2) was found to promote migration and proliferation of epithelial cells under co-culture conditions. These findings provide insights into intercellular communication in prostate cancer that may improve patient management.
Article
Biochemistry & Molecular Biology
Vladimir M. Ryabov, Mikhail M. Baryshev, Mikhail A. Voskresenskiy, Boris V. Popov
Summary: Prostate cancer is a common disease that often starts off as a slow-growing form but can progress into an aggressive and incurable form. Early detection markers are important for predicting this progression. Two preclinical models, PDXs and PDOs, are currently used to study prostate cancer, but they have limitations. This study explores the potential use of primary 2D prostate cell cultures as a preclinical model and identifies promising markers for the transition from indolent to aggressive prostate cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
K. Zhang, H. Zhao, Z. Ji, C. Zhang, P. Zhou, L. Wang, Q. Chen, J. Wang, P. Zhang, Z. Chen, H. H. Zhu, W-Q Gao
Article
Cell & Tissue Engineering
Naitao Wang, Bai-Jun Dong, Yizhou Quan, Qianqian Chen, Mingliang Chu, Jin Xu, Wei Xue, Yi-Ran Huang, Ru Yang, Wei-Qiang Gao
Article
Biochemistry & Molecular Biology
Y. Guo, J. Cui, Z. Ji, C. Cheng, K. Zhang, C. Zhang, M. Chu, Q. Zhao, Z. Yu, Y. Zhang, Y-X Fang, W-Q Gao, H. H. Zhu
Article
Cell Biology
Jia Wang, Ekatherina Batourina, Kerry Schneider, Spenser Souza, Theresa Swayne, Chang Liu, Christopher D. George, Tiffany Tate, Hanbin Dan, Gregory Wiessner, Yelena Zhuravlev, Julie C. Canman, Indira U. Mysorekar, Cathy Lee Mendelsohn
Article
Cell Biology
Yu Zhang, Yongfang Li, Wenqin Luo, Yaohui Tang, Jia Wang, Ru Yang, Wei-Qiang Gao
CELL PROLIFERATION
(2019)
Article
Biotechnology & Applied Microbiology
Chu Mingliang, Zhu Yanqiu, Hu Jianjun, Zhang Zhuxue, Luo Meng, Ma Xiaobo
BIOMED RESEARCH INTERNATIONAL
(2020)
Article
Oncology
Rui Dong, Ran Yang, Yong Zhan, Hua-Dong Lai, Chun-Jing Ye, Xiao-Ying Yao, Wen-Qin Luo, Xiao-Mu Cheng, Ju-Ju Miao, Jun-Feng Wang, Bai-Hui Liu, Xiang-Qi Liu, Lu-Lu Xie, Yi Li, Man Zhang, Lian Chen, Wei-Chen Song, Wei Qian, Wei-Qiang Gao, Yun-Hui Tang, Chun-Yan Shen, Wei Jiang, Gong Chen, Wei Yao, Kui-Ran Dong, Xian-Min Xiao, Shan Zheng, Kai Li, Jia Wang
Article
Cell Biology
Xiaomu Cheng, Huadong Lai, Wenqin Luo, Man Zhang, Juju Miao, Weichen Song, Shunpeng Xing, Jia Wang, Wei-Qiang Gao
Summary: Cyclophosphamide is commonly used for cancer treatment but may cause bladder injury and hemorrhagic cystitis. This study used scRNA-seq to analyze bladder cell types in mice under normal and injured conditions, revealing interactions between superficial and intermediate cells, and identifying two mesenchymal cell lineages.
CELL DEATH & DISEASE
(2021)
Article
Biology
Wenqin Luo, Guan Ning Lin, Weichen Song, Yu Zhang, Huadong Lai, Man Zhang, Juju Miao, Xiaomu Cheng, Yongjie Wang, Wang Li, Wenxiang Wei, Wei-Qiang Gao, Ru Yang, Jia Wang
Summary: This study used single-cell spatial transcriptomics analysis to reveal the similarities and differences between developing cerebellar granule cells and medulloblastoma cells. The research identified different states of developing granule cells and found that transformed granule cells in medulloblastoma closely resemble developing granule neurons but exhibit less tendency to differentiate. This provides direct evidence for the similarities and discrepancies between normal cerebellar development and tumorigenesis.
Article
Oncology
Huadong Lai, Xiaomu Cheng, Qiang Liu, Wenqin Luo, Mengyao Liu, Man Zhang, Juju Miao, Zhongzhong Ji, Guan Ning Lin, Weichen Song, Lianhua Zhang, Juanjie Bo, Guoliang Yang, Jia Wang, Wei-Qiang Gao
Summary: Bladder cancer is a highly heterogeneous disease with distinct histological, molecular, and clinical phenotypes. Single-cell RNA sequencing revealed that transcriptional profiles of tumor cells matched with pathological subtypes. Communication between tumor cells was shown to have an impact on basal/luminal phenotypes.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Review
Biochemistry & Molecular Biology
Sijia Gu, Ling Qian, Yalei Zhang, Kun Chen, Ye Li, Jia Wang, Peng Wang
Summary: The article summarizes the current application of immunotherapy in cancer treatment, focusing on the importance and potential clinical application prospects of tumor-infiltrated B cells in anti-tumor immunity.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2021)
Article
Oncology
Xiying Sun, Yun Zhang, Mingliang Chu, Lixin Wang, Hede Shen, Zhuxue Zhang, Jianjun Hu, Wei Yi, Wenxiu Yang, Xiaobo Ma
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
(2018)
Article
Oncology
Qian Wu, Mingliang Chu, Jianjun Hu, Zhuxue Zhang, Wei Yi, Xiaobo Ma
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
(2018)
Article
Oncology
Yanjing Guo, Kai Zhang, Chaping Cheng, Zhongzhong Ji, Xue Wang, Minglei Wang, Mingliang Chu, Dean G. Tang, Helen He Zhu, Wei-Qiang Gao
CLINICAL CANCER RESEARCH
(2017)
Article
Oncology
Zhuxue Zhang, Mingliang Chu, Jianjun Hu, Wei Yi, Bowen Wang, Xiaobo Ma
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
(2017)