Article
Cell Biology
Meaghan E. Beckner, Lauren Thompson, Patrick N. Radcliffe, Rebecca Cherian, Marques Wilson, Nicholas Barringer, Lee M. Margolis, J. Philip Karl
Summary: This study aimed to identify sex differences in changes in serum metabolome related to body composition, physical performance, and endocrine and metabolic status during a 17-day military training exercise. The results showed that women preferentially mobilize fat stores compared to men during sustained military training, which may help mitigate the loss of lean mass and lower body power.
PHYSIOLOGICAL GENOMICS
(2023)
Article
Chemistry, Applied
Xiao Chen, Zixuan Gu, Yinghan Peng, Siew Young Quek
Summary: Camembert cheese undergoes biochemical changes during ripening, with lipolysis and proteolysis being the primary events. Flavor compounds were also found to contribute to the unique aroma. The ripening process was characterized by an increase in proteolysis and the development of odorants. Specific volatile markers were identified to differentiate the ripening stages of Camembert cheese.
Article
Chemistry, Applied
Andrea Asensio-Grau, Joaquim Calvo-Lerma, Ana Heredia, Ana Andres
Summary: The study found that processing techniques and intestinal conditions have different effects on the digestion of salmon. Protein digestion was most affected by processing, while intestinal conditions were the major determinant of fat digestion.
Article
Food Science & Technology
Shuwen Zhang, Tong Wang, Yumeng Zhang, Bo Song, Xiaoyang Pang, Jiaping Lv
Summary: The use of Monascus as an adjunct starter in the production of Camembert-type cheese was found to enhance proteolysis, lipolysis, and volatile compounds, resulting in improved flavor and taste.
Article
Endocrinology & Metabolism
Ming Gao, Minjian Chen, Qiuzhen Chen, Shuai Zhu, Hengjie Wang, Weizheng Yang, Xi Wang, Qiang Wang, Ling Gu
Summary: Well-controlled metabolism is crucial for optimal oocyte development. This study investigated the intrinsic regulation of oocyte maturation through metabolomics and transcriptomics analysis, revealing novel metabolic features during porcine oocyte meiosis. The findings provide a comprehensive data resource and potential molecular biomarkers for predicting and improving oocyte quality.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Roberto Stella, Raphael Severino Bonadio, Stefano Cagnin, Maria Lina Massimino, Alessandro Bertoli, Caterina Peggion
Summary: This study investigated the proteomic profile and secreted metabolome of ALS astrocytes derived from transgenic mouse models, revealing depleted proteins and secreted metabolites involved in glutathione metabolism and signaling, as well as altered transcription factor activation and lipid metabolism in ALS astrocytes expressing mutated hSOD1. These findings provide insights into potential therapeutic targets to address astrocyte dysfunctionalities and altered astrocyte-MN crosstalk in ALS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Physiology
Yingjiang Xu, Huan Liu, Dianfeng Han, Lihua Ren, Xianghong Gong, Fang Jiang, Yanmei Cui, Xiaojing Liu, Chuanbo Ren, Jinglin Xue, Xiuhui Tian
Summary: The study revealed that the metabolic changes in the digestive system of mantis shrimp under Cd stress, with 91 metabolites differentially expressed mainly in amino acids, phospholipids, and fatty acid esters. The functional pathways of differential metabolites showed significant effects on biological processes such as amino acid metabolism, transmembrane transport, energy metabolism, and signal transduction. The Cd-induced oxidative stress and energy metabolism disorders were characterized by the differential expression of amino acids and ADP in mantis shrimp, while the interference of transmembrane transport and signal transduction was due to the differential expression of phospholipids.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Food Science & Technology
Oceance Savary, Emmanuel Coton, Marie-Bernadette Maillard, Frederic Gaucheron, Christophe Le Meur, Jens Frisvad, Anne Thierry, Jean-Luc Jany, Monika Coton
Summary: This study aimed to evaluate the functional impact of Bisifusarium domesticum and other cheese-associated fungi on cheese-making. Their lipolytic and proteolytic activities, as well as their potential to produce volatile and non-volatile secondary metabolites, were assessed. Four novel cheese-associated species, including Bisifusarium allantoides, Bisifusarium penicilloides, Longinectria lagenoides, and Long-inectria verticilliformis, were described, and these species showed potential in lipid production.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Nutrition & Dietetics
Longwei Xi, Gang Zhai, Yulong Liu, Yulong Gong, Qisheng Lu, Zhimin Zhang, Haokun Liu, Junyan Jin, Xiaoming Zhu, Zhan Yin, Shouqi Xie, Dong Han
Summary: This study blocked glucose uptake by knocking out glut2 in zebrafish and found that impaired insulin signaling-mediated anabolism and enhanced AMPK signaling-mediated catabolism contribute to energy homeostasis remodeling caused by blocked glucose uptake. These findings reveal the mechanism of energy homeostasis remodeling and provide a potential strategy for adapting to low glucose levels.
FRONTIERS IN NUTRITION
(2023)
Article
Food Science & Technology
James A. Elegbeleye, Elna M. Buys
Summary: This study aimed to quantify and compare biofilm-induced proteolysis and lipolysis in Bacillus subtilis and Bacillus velezensis with planktonic cells. Differences were observed in cell counts and enzyme concentrations in biofilms and planktonic cells in UHT milk. The spoilage potential of biofilms and planktonic cultures was found to be strain-dependent with variations in proteolysis and lipolysis among different strains.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Aurelie Thooft, Raphael Conotte, Jean-Marie Colet, Karim Zouaoui Boudjeltia, Patrick Biston, Michael Piagnerelli
Summary: Inflammatory processes in ICU patients can lead to metabolic changes and increased risks of morbidity and mortality. This study aimed to determine if metabolomics can aid in prognosis at ICU admission. Metabolic profiles of ICU patients were compared to healthy volunteers, and differences were found in septic shock patients. However, these metabolite profiles were not correlated with mortality. Changes in metabolic products were observed in septic shock patients on the first day of ICU admission, but these changes did not predict prognosis.
Article
Biochemistry & Molecular Biology
Dongqin Wei, Qian Sun, Yizhou Li, Chaowei Li, Xinjian Li, Chao Sun
Summary: The study revealed that leptin can reduce the m(6)A methylation of Plin5 by promoting FTO, affecting lipid metabolism and energy consumption, providing a theoretical basis for treating diseases related to obesity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Si-Lan Han, Yan Liu, Samwel M. Limbu, Li-Qiao Chen, Mei-Ling Zhang, Zhen-Yu Du
Summary: In this study, Atglistatin, an ATGL-specific inhibitor, was added to the zebrafish diet and led to severe fat deposition, reduced oxygen consumption, oxidative stress, and inflammation. The Atglistatin-treated fish showed elevated HSL protein expression but reduced free fatty acids and lipase activities, as well as decreased autophagy marker. Glycogenolysis was stimulated while blood glucose was higher in the Atglistatin-treated fish, indicating a loss of lipid-sourced energy production due to ATGL inhibition.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Environmental Sciences
Ce Shi, Yangfang Ye, Feng Pei, Changkao Mu, Chunlin Wang
Summary: The study found that using a transport bag containing oxygen and seawater can effectively extend the post-capture survival time of small swimming crabs, possibly due to metabolic adaptation including a switch in energy generation pathway and decline in certain metabolic processes. Energy homeostasis is crucial for crab survival during transport.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Food Science & Technology
Mariam Sardinas-Valdes, Hugo Sergio Garcia-Galindo, Alfonso Juventino Chay-Canul, Jose Rodolfo Velazquez-Martinez, Josafat Alberto Hernandez-Becerra, Angelica Alejandra Ochoa-Flores
Summary: The addition of nano-emulsified curcumin to hair sheep milk resulted in cheeses with increased fat and ash levels, as well as higher total phenolic content and antioxidant activity during ripening. Lipolysis increased steadily in the first 40 days of ripening, while proteolysis increased consistently throughout the ripening process. This study highlights the potential of using nano-emulsified curcumin to enhance the functionality of dairy foods.
Article
Pediatrics
Timothy Vigers, Carissa Vinovskis, Lu-Ping Li, Pottumarthi Prasad, Hiddo Heerspink, Angelo D'Alessandro, Julie A. Reisz, Federica Piani, David Z. Cherney, Daniel H. van Raalte, Kristen J. Nadeau, Meda E. Pavkov, Robert G. Nelson, Laura Pyle, Petter Bjornstad
Summary: This study compared plasma metabolites between youth with and without type 1 diabetes mellitus (T1DM) and found lower levels of metabolites associated with amino acid oxidation and the tricarboxylic acid cycle in individuals with T1DM. These metabolites were related to kidney function and may serve as potential biomarkers for improving kidney bioenergetics.
PEDIATRIC NEPHROLOGY
(2023)
Article
Critical Care Medicine
Margot DeBot, Christopher Erickson, Marguerite Kelher, Terry R. Schaid, Ernest E. Moore, Angela Sauaia, Alexis Cralley, Ian LaCroix, Angelo D'Alessandro, Kirk Hansen, Mitchell J. Cohen, Christopher C. Silliman, Julia Coleman
Summary: By studying the sex differences in platelets and cryoprecipitate coagulation factors, it was found that blood products from female donors are more effective in improving coagulation dysfunction compared to male blood products. Additionally, female-donor cryoprecipitate contains more factor V and factor XIII, and several proteins with potential immunological significance. These sex differences may influence how transfusions modulate thromboinflammation caused by trauma, suggesting the potential role of sex-specific transfusion guidelines in hemostatic resuscitation.
JOURNAL OF TRAUMA AND ACUTE CARE SURGERY
(2023)
Article
Critical Care Medicine
Terry R. Schaid, Ian LaCroix, Kirk C. Hansen, Angelo D'Alessandro, Ernest E. Moore, Angela Sauaia, Monika Dzieciatkowska, Margot DeBot, Alexis L. Cralley, Otto Thielen, William Hallas, Christopher Erickson, Sanchayita Mitra, Anirban Banerjee, Kenneth Jones, Christopher C. Silliman, Mitchell J. Cohen
Summary: This study identified the proteomic signature of trauma-induced NETosis and revealed that NETosis may mediate organ dysfunction following severe injury. SerpinB1 was identified as a major component of this NET protein profile and could serve as an early marker of excessive NETosis after injury.
JOURNAL OF TRAUMA AND ACUTE CARE SURGERY
(2023)
Article
Hematology
Angelo D'Alessandro, S. Mehdi Nouraie, Yingze Zhang, Francesca Cendali, Fabia Gamboni, Julie A. Reisz, Xu Zhang, Kyle W. Bartsch, Matthew D. Galbraith, Victor R. Gordeuk, Mark T. Gladwin
Summary: This study evaluates the red blood cell metabolism of 587 sickle cell disease patients and finds significant metabolic alterations in RBC acylcarnitines, pyruvate, sphingosine 1-phosphate, creatinine, kynurenine, and urate in Hb SS genotype compared to AA and SC RBCs. Surprisingly, the metabolism of SC RBCs is dramatically different from SS RBCs, with all glycolytic intermediates significantly elevated. These findings provide insights into the metabolic signatures and potential underlying mechanisms in sickle cell disease.
AMERICAN JOURNAL OF HEMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ji Young Lee, Reece P. Stevens, Viktoriya V. Pastukh, Viktor M. Pastukh, Natalya Kozhukhar, Mikhail F. Alexeyev, Julie A. Reisz, David Nerguizian, Angelo D'Alessandro, Anna Koloteva, Meredith S. Gwin, Justin T. Roberts, Glen M. Borchert, Brant M. Wagener, Jean-Franc Comma Ois Pittet, Brian B. Graham, Kurt R. Stenmark, Troy Stevens
Summary: Pulmonary microvascular endothelial cells prefer glucose over fructose for glycolysis, and this study found that the enzyme PFKFB3 inhibits fructose metabolism in these cells. PFKFB3 knockout led to increased survival and enhanced fructose utilization in fructose-rich medium. The study also demonstrated that pneumonia is associated with increased fructose levels in the lung. These findings suggest that PFKFB3 is a key regulator of glucose versus fructose use in glycolysis in pulmonary microvascular endothelial cells.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2023)
Article
Oncology
Miles Piper, Maureen Hoen, Laurel B. Darragh, Michael W. Knitz, Diemmy Nguyen, Jacob Gadwa, Greta Durini, Idil Karakoc, Abby Grier, Brooke Neupert, Benjamin Van Court, Khalid N. M. Abdelazeem, Justin Yu, Nicholas A. Olimpo, Sophia Corbo, Richard Blake Ross, Tiffany T. Pham, Molishree Joshi, Ross M. Kedl, Anthony J. Saviola, Maria Amann, Pablo Umana, Laura Codarri Deak, Christian Klein, Angelo D'Alessandro, Sana D. Karam
Summary: In PDAC patients, the response to RT involves changes in IL-2Rb, IL-2Rg, and IL-2Ra expression. PD1-IL2v, a PD-1-targeted IL-2 variant immunocytokine, enhances tumor-specific T cell activation and reduces Treg suppression. In PDAC mouse models, PD1-IL2v treatment improves survival, increases tumor-infiltrating CD8+ T cells, and activates antigen-specific CD8+ T cells, especially when combined with single-dose RT.
Article
Biochemistry & Molecular Biology
Giulia Unali, Giovanni Crivicich, Isabel Pagani, Monah Abou-Alezz, Filippo Folchini, Erika Valeri, Vittoria Matafora, Julie A. Reisz, Anna Maria Sole Giordano, Ivan Cuccovillo, Giacomo M. Butta, Lorena Donnici, Angelo D'Alessandro, Raffaele De Francesco, Lara Manganaro, Davide Cittaro, Ivan Merelli, Carolina Petrillo, Angela Bachi, Elisa Vicenzi, Anna Kajaste-Rudnitski
Summary: The interferon-induced transmembrane proteins (IFITM) inhibit endocytic viral entry by binding to phosphatidylinositol 3,4,5-trisphosphate (PIP3), which plays a key role in endosomal antiviral immunity. The level of PIP3 is closely correlated with the potency of endosomal IFITM restriction, and exogenous PIP3 enhances the inhibition of endocytic viruses. These findings elucidate cell-compartment-specific antiviral mechanisms and have potential relevance for the development of broadly acting antiviral strategies.
Article
Pharmacology & Pharmacy
Hui Zhang, Angelo D'Alessandro, Min Li, Julie A. Reisz, Suzette Riddle, Akshay Muralidhar, Todd Bull, Lan Zhao, Evgenia Gerasimovskaya, Kurt R. Stenmark
Summary: This study investigates the effects of sildenafil on fibroblasts derived from patients with pulmonary hypertension (PH). Sildenafil showed specific effects on purine metabolites, especially adenosine, adenine, and xanthine. However, its impact on cell stress markers was limited. Combination treatment of sildenafil with HDAC inhibitors showed promising inhibitory effects on proliferation and metabolic reprogramming in PH-fibroblasts.
VASCULAR PHARMACOLOGY
(2023)
Article
Multidisciplinary Sciences
Jacqueline A. Turner, Malia A. Fredrickson, Marc D'Antonio, Elizabeth Katsnelson, Morgan MacBeth, Robert Van Gulick, Tugs-Saikhan Chimed, Martin McCarter, Angelo D'Alessandro, William A. Robinson, Kasey L. Couts, Roberta Pelanda, Jared Klarquist, Richard P. Tobin, Raul M. Torres
Summary: Lysophosphatidic acid (LPA) increases in concentration in various cancer types and has an impact on CD8 T cell metabolism, phenotype, and effector functions. Plasma concentrations of LPA can predict the response to immunotherapy.
NATURE COMMUNICATIONS
(2023)
Article
Developmental Biology
Hiroko Kawakami, Katherine Q. Chen, Ruizhi Zhang, Matthew P. Pappas, Abigail Bailey, Julie A. Reisz, Dylan Corcoran, Ryuichi Nishinakamura, Angelo D'Alessandro, Yasuhiko Kawakami
Summary: Recent studies have shown that regulation of cellular metabolism, especially glycolysis and pathways branching from glycolysis, plays a crucial role in vertebrate embryo development. The transcription factor Sall4 is highly expressed in undifferentiated cells in developing mouse embryos and is involved in the regulation of glycolytic metabolism. Knockout experiments demonstrate that Sall4 restricts glycolysis in limb buds and is important for hindlimb patterning.
DEVELOPMENTAL BIOLOGY
(2023)
Article
Hematology
Ian S. LaCroix, Mitchell Cohen, Ernest E. Moore, Monika Dzieciatkowska, Christopher C. Silliman, Kirk C. Hansen, Angelo D'Alessandro
Summary: This study characterized the multi-omics features of platelet transfusion efficacy in a clinically relevant cohort of trauma patients using metabolomics and proteomics approaches. The results showed higher levels of tranexamic acid, inflammatory proteins, carnitines, and polyamines in patients requiring platelet transfusion. The study also identified increased platelet activation in the most severely injured patients. This study provides the first multi-omics characterization of platelet transfusion efficacy.
Review
Hematology
Angelo D'Alessandro, Eldad A. Hod
Summary: In the past decade, omics technologies, especially high-throughput genomics and metabolomics, have greatly contributed to our understanding of the role of donor genetics and nongenetic determinants in red blood cell storage biology. These technologies have shed light on the impact of factors such as donor sex, age, ethnicity, processing strategies, and environmental or dietary exposures on the onset and severity of storage lesion. Recent advances have also demonstrated the influence of genetically encoded polymorphisms on red cell storage biology and its correlation with storage quality and post-transfusion efficacy. The combination of these novel technologies has the potential to drive personalized transfusion medicine approaches.
TRANSFUSION MEDICINE REVIEWS
(2023)
Article
Nutrition & Dietetics
Christopher Y. Kim, Hannah J. Larsen, Steven L. Spitalnik, Eldad A. Hod, Richard O. Francis, Krystalyn E. Hudson, Dominique E. Gordy, Elizabeth F. Stone, Sandy Peltier, Pascal Amireault, Angelo D'Alessandro, James C. Zimring, Paul W. Buehler, Xiaoyun Fu, Tiffany Thomas
Summary: High-dose fish oil intake reduces the quality of red blood cells, while low-dose fish oil improves the filterability of fresh red blood cells and reduces lipid peroxidation of stored red blood cells. However, low-dose fish oil does not improve the post-transfusion recovery capacity of stored red blood cells.
Review
Biochemistry & Molecular Biology
Alicia Key, Zachary Haiman, Bernhard O. Palsson, Angelo D'Alessandro
Summary: Red blood cells are an abundant and relatively simple type of cells in the human body. They have been used as an ideal model for studying metabolism due to their accessibility. Computational models have the potential to enhance our understanding of red blood cell metabolism and predict their metabolic behaviors. Metabolomics and systems biology have provided evidence to advance our understanding of the storage lesion in red blood cells.
Article
Chemistry, Analytical
Francesca Cendali, Angelo D'Alessandro, Travis Nemkov
Summary: Metabolomics and lipidomics techniques can comprehensively measure small molecules, and advanced microsampling devices offer a convenient way to monitor metabolomic and lipidomic changes during exercise. In this study, significant changes in energy and redox metabolism were observed in both male and female volunteers, while lipid changes were more individualized. This approach showcases the ability to monitor circulating metabolome and lipidome profiles in response to exercise.
ANALYTICAL SCIENCE ADVANCES
(2023)