Article
Multidisciplinary Sciences
Ewan A. Ross, Lesley-Anne Turner, Hannah Donnelly, Anwer Saeed, Monica P. Tsimbouri, Karl V. Burgess, Gavin Blackburn, Vineetha Jayawarna, Yinbo Xiao, Mariana A. G. Oliva, Jennifer Willis, Jaspreet Bansal, Paul Reynolds, Julia A. Wells, Joanne Mountford, Massimo Vassalli, Nikolaj Gadegaard, Richard O. C. Oreffo, Manuel Salmeron-Sanchez, Matthew J. Dalby
Summary: This study reveals that nanotopography can regulate the immunomodulatory capacity of MSCs by reducing intracellular tension and increasing oxidative glycolysis, providing an effective approach for large-scale expansion of functional MSCs for therapeutic purposes.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Sarah Adamo, Jan Michler, Yves Zurbuchen, Carlo Cervia, Patrick Taeschler, Miro E. Raeber, Simona Baghai Sain, Jakob Nilsson, Andreas E. Moor, Onur Boyman
Summary: Researchers used spectral flow cytometry combined with cellular indexing of transcriptomes and T cell receptor sequencing to longitudinally characterize individual SARS-CoV-2-specific CD8(+) T cells from patients with COVID-19, identifying a distinct signature marking long-lived memory CD8(+) T cells. The study found that SARS-CoV-2-specific memory CD8(+) T cells persisting 1 year after acute infection express specific markers and exhibit characteristics resembling effector memory T cells, and that certain transcriptional signatures and signaling pathways are associated with the fate of individual clones of these cells.
Article
Multidisciplinary Sciences
Azzurra Codino, Tomasz Turowski, Louie N. van de Lagemaat, Ivayla Ivanova, Andrea Tavosanis, Christian Much, Tania Auchynnikava, Lina Vasiliauskaite, Marcos Morgan, Juri Rappsilber, Robin C. Allshire, Kamil R. Kranc, David Tollervey, Donal O'Carroll
Summary: The RNA-binding protein NANOS2 plays a crucial role in maintaining spermatogonial stem cells (SSCs) by recognizing specific consensus motifs in the 3' untranslated region of target mRNAs. NANOS2 interacts with the CCR4-NOT deadenylase complex in SSCs, reducing the stability of target transcripts and affecting key self-renewal pathways related to SSC maintenance.
Article
Biochemistry & Molecular Biology
Eun-Ju Lee, Khurshid Ahmad, Shiva Pathak, SunJu Lee, Mohammad Hassan Baig, Jee-Heon Jeong, Kyung-Oh Doh, Dong-Mok Lee, Inho Choi
Summary: This study introduces novel peptides derived from fibronectin that enhance cell adhesion, proliferation, and differentiation, offering a new approach to cell culture. The peptides showed significant effects on primary cells and stem cells, increasing proliferation and promoting differentiation into specific cell types such as osteoblasts. The results suggest potential applications in the production of core materials for cell culture such as scaffolds and culture medium components.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Benjamin Boumard, Allison J. Bardin
Summary: Stem cells play essential roles in organ development and maintenance, and understanding the mechanisms by which tissue-specific stem cells renew tissues is a key goal of regenerative medicine. The Drosophila midgut provides a widely used model system for studying regulatory mechanisms of stem cell biology.
CURRENT OPINION IN CELL BIOLOGY
(2021)
Article
Immunology
Duancheng Guo, Yuan Wang, Yan Cheng, Shengyou Liao, Jian Hu, Fang Du, Gang Xu, Yongqiang Liu, Kathy Q. Cai, Martin Cheung, Brandon J. Wainwright, Q. Richard Lu, Yi Zhao, Zeng-jie Yang
Summary: The research demonstrates that astrocytes in relapsed MB mainly originate from the transdifferentiation of tumor cells, which depends on Sox9 and the BMP signaling pathway. Inhibiting BMP signaling pharmacologically suppresses the transdifferentiation of MB cells into astrocytes, thus suppressing tumor relapse.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Psychology, Developmental
Joan Rosenbaum Asarnow, Michele S. Berk, Jamie Bedics, Molly Adrian, Robert Gallop, Judith Cohen, Kathryn Korslund, Jennifer Hughes, Claudia Avina, Marsha M. Linehan, Elizabeth McCauley
Summary: This study evaluated mechanisms, mediation, and secondary/exploratory outcomes in our randomized controlled trial comparing dialectical behavior therapy (DBT) to individual and group supportive therapy (IGST). Results showed that DBT led to greater improvements in youth emotion regulation during treatment and throughout the 12-month study period, as well as higher rates of self-harm remission during follow-up compared to IGST. The study also found that improvements in youth emotion regulation during treatment mediated the association between DBT and self-harm remission during follow-up.
JOURNAL OF THE AMERICAN ACADEMY OF CHILD AND ADOLESCENT PSYCHIATRY
(2021)
Article
Multidisciplinary Sciences
Ritsuko Morita, Noriko Sanzen, Hiroko Sasaki, Tetsutaro Hayashi, Mana Umeda, Mika Yoshimura, Takaki Yamamoto, Tatsuo Shibata, Takaya Abe, Hiroshi Kiyonari, Yasuhide Furuta, Itoshi Nikaido, Hironobu Fujiwara
Summary: This study utilized marker-independent long-term 3D live imaging and single-cell transcriptomics to investigate the development of epithelial lineage in mouse hair follicles. The findings revealed that precursors of different epithelial lineages align in a 2D concentric manner and extend to form longitudinally aligned, 3D cylindrical compartments. The fate of placode cells is determined by cell position rather than the orientation of cell division.
Article
Nanoscience & Nanotechnology
Shreya Udani, Justin Langerman, Doyeon Koo, Sevana Baghdasarian, Brian Cheng, Simran Kang, Citradewi Soemardy, Joseph de Rutte, Kathrin Plath, Dino Di Carlo
Summary: By developing secretion-encoded single-cell sequencing (SEC-seq) method, the authors simultaneously measured the secretion and transcriptome for thousands of cells, and found heterogeneity in the secretion of vascular endothelial growth factor A (VEGF-A) within the cell population, which is poorly correlated with the VEGFA transcript level. Enrichment of a specific cell subpopulation characterized by a unique gene expression signature enabled the identification of gene signatures linked to specific secretory states. SEC-seq facilitates mechanistic studies and the development of means to modulate cellular secretion.
NATURE NANOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Kennosuke Ichikawa, Yoshiaki Nakamura, Hidemasa Bono, Ryo Ezaki, Mei Matsuzaki, Hiroyuki Horiuchi
Summary: Research on male and female primordial germ cells in chickens showed that female-biased genes are enriched in metabolic processes in gonadal PGCs. Stimulation tests with retinoic acid revealed transcriptional regulation mechanisms of female-biased genes. It suggests that sex determination in avian PGCs involves both cell-autonomous and somatic-cell regulation, with females determining sex earlier than males.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Hui Song Cui, So Young Joo, Seung Yeol Lee, Yoon Soo Cho, Dong Hyun Kim, Cheong Hoon Seo
Summary: It is unclear how scar fibroblasts (SFs) affect keratinocytes in hypertrophic scars (HTS) through cell-cell interaction. This study investigated the effects of HTS-derived exosomes on the proliferation and differentiation of normal human keratinocytes (NHKs) and compared them with normal fibroblasts (NFs).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Tanachapa Jantalika, Sirikul Manochantr, Pakpoom Kheolamai, Duangrat Tantikanlayaporn, Weerachai Saijuntha, Somchai Pinlaor, Arthit Chairoungdua, Luminita Paraoan, Chairat Tantrawatpan
Summary: This study found that conditioned media derived from human chorion-derived mesenchymal stem cells (CH-MSCs) have an inhibitory effect on the proliferation and a pro-apoptotic effect on cholangiocarcinoma cells. CH-CM treatment induced a decrease in mitochondrial membrane potential and exerted its effects through the inhibition of the JAK2/STAT3 signaling pathway, downregulation of BCL-2 expression, and upregulation of BAX expression.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Michael Pryszlak, Mallory Wiggans, Xin Chen, Julia E. Jaramillo, Sarah E. Burns, Laura M. Richards, Trevor J. Pugh, David R. Kaplan, Xi Huang, Peter B. Dirks, Bret J. Pearson
Summary: By using a comparative genomics approach, researchers have identified and demonstrated the role of DEAD-box helicase DDX56 in regulating stemness in different cell systems. DDX56 affects ribosomal RNA expression and nucleolar integrity in planarians, mouse neural stem cells, human GSCs, and a fruit fly model, ultimately leading to stem cell death.
Article
Cell Biology
Shan Wang, Cheng Shi, Xiaohui Cai, Yanbin Wang, Xi Chen, Hongjing Han, Huan Shen
Summary: The study showed that UCMSC-AAM transplantation can significantly improve endometrial thickness and biomarker expression in rat models with endometrial injury. Additionally, the expression of inflammatory factors decreased and that of anti-inflammatory factors increased in the UCMSC-AAM group.
MEDIATORS OF INFLAMMATION
(2021)
Article
Toxicology
Thunwa Binlateh, Ratchaneekorn Reudhabibadh, Peerada Prommeenate, Pilaiwanwadee Hutamekalin
Summary: This study suggests that metformin inhibits the proliferation and growth of neuroblastoma through the Erk1/2 and Cdk5 pathways, induced by morphological differentiation. Metformin may be considered as a potential differentiation agent for neuroblastoma treatment.
TOXICOLOGY IN VITRO
(2022)
Meeting Abstract
Oncology
Xiaorong Gu, Reda Mahfouz, Ji Zhang, Francis Enane, Zhenbo Hu, Tomas Radivoyevitch, David Wald, Maria Paola Martell, Brunangelo Falini, Yosef Landesman, Jaroslaw Maciejewski, Yogen Saunthararajah
Article
Medicine, Research & Experimental
Francis O. Enane, Wai Ho Shuen, Xiaorong Gu, Ebrahem Quteba, Bartlomiej Przychodzen, Hideki Makishima, Juraj Bodo, Joanna Ng, Chit Lai Chee, Rebecca Ba, Lip Seng Koh, Janice Lim, Rachael Cheong, Marissa Teo, Zhenbo Hu, Kwok Peng Ng, Jaroslaw Maciejewski, Tomas Radivoyevitch, Alexander Chung, London Lucien Ooi, Yu Meng Tan, Peng-Chung Cheow, Pierce Chow, Chung Yip Chan, Kiat Hon Lim, Lisa Yerian, Eric Hsi, Han Chong Toh, Yogen Saunthararajah
JOURNAL OF CLINICAL INVESTIGATION
(2017)
Meeting Abstract
Oncology
Francis O. Enane, Marissa Teo, Hideki Makishima, Zhenbo Hu, Han Chong Toh, Yogen Saunthararajah, Joanna Ng, Chit Lai, Soo Fan Ang, Lim Kiat Hon
Article
Medicine, Research & Experimental
Xiaorong Gu, Quteba Ebrahem, Reda Z. Mahfouz, Metis Hasipek, Francis Enane, Tomas Radivoyevitch, Nicolas Rapin, Bartlomiej Przychodzen, Zhenbo Hu, Ramesh Balusu, Claudiu Cotta, David Wald, Christian Argueta, Yosef Landesman, Maria Paola Martelli, Brunangelo Falini, Hetty Carraway, Bo T. Porse, Jaroslaw Maciejewski, Babal K. Jha, Yogen Saunthararajah
JOURNAL OF CLINICAL INVESTIGATION
(2018)
Article
Cell Biology
Xiaorong Gu, Francis Enane, Rita Tohme, Caroline Schuerger, Tomas Radivoyevitch, Yvonne Parker, Eric Zuberi, Bartlomiej Przychodzen, Babal Kant Jha, Daniel Lindner, Brian Rini, Yogen Saunthararajah
Summary: The study found that PAX8 recruits PBRM1/PBAF in clear cell renal cell cancers (RCCs) and corepressors are more conspicuous in RCC cells, opposing the function of coactivators. Key PAX8 target genes are repressed in RCC compared to normal kidneys, and reintroduction of PBRM1 or depletion of opposing corepressors can correct the coregulator imbalance.
Meeting Abstract
Oncology
Xiaorong Gu, Francis Enane, Reda Mahfouz, Tomas Radivoyevitch, Bartlomiej Przychodzen, Yvonne Parker, Daniel Lindner, Brian Rini, Yogen Saunthararajah
Meeting Abstract
Biochemistry & Molecular Biology
Maria O. Longas, Jennifer Trinkle-Pereira, Kenya Cheairs, Timothy O. Ojo, Francis Enane, David Warner