Article
Multidisciplinary Sciences
Ojore Benedict Valentine Oka, Arvin Shedrach Pierre, Marie Anne Pringle, Wanida Tungkum, Zhenbo Cao, Bethany Fleming, Neil John Bulleid
Summary: The unfolded protein response (UPR) regulates cellular proteostasis by activating and trafficking the ATF6 alpha sensor. After ER stress, ATF6 alpha undergoes a redox switch to form a disulfide bonded dimer, which is then transported to the Golgi for cleavage. Overexpression of ERp18 affects dimer formation and limits Golgi trafficking.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Siqi Cai, Zezhong Zheng, JiaoJiao Cheng, Lintao Zhong, Ran Shao, Feiyan Zheng, Zhiying Lai, Jiajun Ou, Liang Xu, Pei Zhou, Gang Lu, Guihong Zhang
Summary: This study identified swine interferon-induced transmembrane proteins (SwIFITMs) as having potent antiviral activity against African swine fever virus (ASFV). These proteins were found to be constitutively expressed in most porcine tissues and their expression levels were induced by ASFV infection or swine interferon treatment. Overexpression of SwIFITMs inhibited ASFV replication in Vero cells, while knockdown of these genes enhanced ASFV replication in PAMs. The study also demonstrated that endogenous IFITMs play a role in restricting ASFV replication. Overall, this research expands our understanding of the antiviral activity of porcine IFITMs against ASFV.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biology
Zhaoyao Li, Wenxian Chen, Zilong Qiu, Yuwan Li, Jindai Fan, Keke Wu, Xiaowen Li, Mingqiu Zhao, Hongxing Ding, Shuangqi Fan, Jinding Chen
Summary: African swine fever is a highly fatal viral disease in domestic pigs and wild boars, impacting pig-raising countries and pork trade. Safe and effective vaccines are urgently needed to control outbreaks. Understanding the structure, function, immune response, and diagnosis of the ASF virus is crucial for vaccine development.
Article
Infectious Diseases
Ulaankhuu Ankhanbaatar, Tserenchimed Sainnokhoi, Buyantogtokh Khanui, Gerelmaa Ulziibat, Tserenjav Jargalsaikhan, Dulam Purevtseren, Tirumala Bharani K. Settypalli, John Flannery, William G. Dundon, Ganzorig Basan, Carrie Batten, Giovanni Cattoli, Charles E. Lamien
Summary: African swine fever (ASF) is a severe haemorrhagic disease of pigs caused by the ASFV virus, which has spread to Europe and Asia. Mongolia reported its first outbreak on January 15th, 2019, becoming the second country in the region affected by the disease after China. The Mongolian ASFV belong to genotype II and serogroup 8, indicating a possible cross-border spread of the virus.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Virology
Nengwen Xia, Hui Wang, Xueliang Liu, Qi Shao, Da Ao, Yulin Xu, Sen Jiang, Jia Luo, Jiajia Zhang, Nanhua Chen, Francois Meurens, Wanglong Zheng, Jianzhong Zhu
Summary: The study revealed that p17 protein inhibits cell proliferation through inducing cell cycle arrest at the G2/M phase. Additionally, p17 triggers oxidative stress and increases intracellular reactive oxygen species (ROS) levels. Mitigating ER stress reduces ROS production and prevents the decrease in cell proliferation caused by p17.
Review
Immunology
Xianghan Duan, Yi Ru, Wenping Yang, Jingjing Ren, Rongzeng Hao, Xiaodong Qin, Dan Li, Haixue Zheng
Summary: African swine fever is a highly contagious disease that threatens the pig industry worldwide, with no effective vaccines or drugs. The complex genome of ASFV and the structure and infection process of the viral proteins are still not fully understood.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Yuxuan Zheng, Su Li, Shi-Hua Li, Shaoxiong Yu, Qihui Wang, Kehui Zhang, Liang Qu, Yuan Sun, Yuhai Bi, Fuchou Tang, Hua-Ji Qiu, George F. Gao
Summary: African swine fever virus (ASFV) infection leads to increased expression of viral transmembrane genes, activation of antiviral signaling pathways and inflammatory response. Cells infected with different viral loads show distinct transcriptomic changes during infection. Furthermore, ASFV infection activates the cell apoptosis pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Infectious Diseases
Jordan T. Gebhardt, Steve S. Dritz, C. Grace Elijah, Cassandra K. Jones, Chad B. Paulk, Jason C. Woodworth
Summary: The diagnostic investigation highlighted contamination of ASFV on feed trucks and within the feed production system, emphasizing the importance of biosecurity measures, particularly in managing employee traffic. The report underscores the significance of routine data capture for evaluating the efficacy of biosecurity procedures.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Veterinary Sciences
Taehwan Oh, Duy Tien Do, Hung Van Vo, Hyeok-il Kwon, Seung-Chul Lee, Min Ho Kim, Dung Thi Thu Nguyen, Quang Tin Vinh Le, Tan Minh Tran, Toan Tat Nguyen, Joo Young Lee, Chanhee Chae
Summary: The use of renal-derived swine macrophages as a novel primary cell candidate offers a solution to ethical constraints and consistency problems in ASFV propagation. These primary cells are proven to be permissive to both cell adapted ASFV and wild-type ASFV, making them useful for virus isolation and propagation. Additionally, compared to commercial cell lines, renal-derived macrophages demonstrate consistent molecular characteristics and are more suitable for isolating field viruses.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Virology
Yanyan Zhang, Junnan Ke, Jingyuan Zhang, Jinjin Yang, Huixian Yue, Xintao Zhou, Yu Qi, Rongnian Zhu, Faming Miao, Qian Li, Fei Zhang, Ying Wang, Xun Han, Lijuan Mi, Jinmei Yang, Shoufeng Zhang, Teng Chen, Rongliang Hu
Summary: African swine fever (ASF) is a severe infectious disease in pigs caused by ASFV, with no commercial vaccine available. A new vaccine candidate named SY18DI226R has been identified, showing promising results in eliciting immunity to virulent ASFV infection in pigs.
JOURNAL OF VIROLOGY
(2021)
Review
Microbiology
Hongliang Zhang, Saisai Zhao, Haojie Zhang, Zhihua Qin, Hu Shan, Xiulei Cai
Summary: African swine fever (ASF) is a fatal infectious disease caused by the ASF virus (ASFV). It is classified as a legally notifiable disease that must be reported to the World Organization for Animal Health. The global pig industry has suffered significant economic losses due to ASF, and controlling and eradicating the disease is crucial. Vaccination is the optimal strategy, but an effective ASF vaccine with high immunoprotective potential is still needed.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Virology
Tingting Li, Gaihong Zhao, Taoqing Zhang, Zhaoxia Zhang, Xin Chen, Jie Song, Xiao Wang, Jiangnan Li, Li Huang, Lili Wen, Changyao Li, Dongming Zhao, Xijun He, Zhigao Bu, Jun Zheng, Changjiang Weng
Summary: ASFV protein pE199L induces mitochondrial-dependent apoptosis by interacting with anti-apoptotic proteins, providing insight into the pathogenesis of African swine fever virus.
Article
Microbiology
Changjie Lv, Jingyu Yang, Li Zhao, Chao Wu, Chao Kang, Qiang Zhang, Xiaomei Sun, Xi Chen, Zhong Zou, Meilin Jin
Summary: This study successfully utilized Bama minipigs as a model for studying ASFV infection in small ABSL-3 laboratories. The results showed that the pathological changes, viral shedding, and gene regulation in ASFV-infected Bama minipigs were consistent with those of domestic pigs infected with ASFV. This model can accelerate research on ASFV vaccines, antiviral drugs, and pathogenic mechanisms.
MICROBIOLOGY SPECTRUM
(2022)
Article
Agriculture, Multidisciplinary
Song Xiang-peng, Xia Ying-ju, Xu Lu, Zhao Jun-jie, Wang Zhen, Zhao Qi-zu, Liu Ye-bing, Zhang Qian-yi, Wang Qin
Summary: With the implementation of the C-strain vaccine, classical swine fever (CSF) has been controlled in China, while African swine fever (ASF) and atypical porcine pestivirus (APPV) still pose challenges to the pig industry. It is crucial to develop a reliable method for simultaneous detection and differential diagnosis of these diseases. In this study, a multiplex real-time PCR assay was established, which showed high sensitivity and specificity for the detection of CSFV, ASFV, and APPV.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Infectious Diseases
Ellen Mighell, Michael P. Ward
Summary: By conducting spatio-temporal analysis on the early spread of African Swine Fever Virus in Asia, the study identified the primary pathways and trends of transmission, as well as epidemiological differences and risk factors among different countries.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Pharmacology & Pharmacy
I Galindo, U. Garaigorta, F. Lasala, M. A. Cuesta-Geijo, P. Bueno, C. Gil, R. Delgado, P. Gastaminza, C. Alonso
Summary: The study demonstrates that drug compounds targeting endosomal membrane proteins show potent inhibitory effects on the entry of SARS-CoV-2, EBOV, and ASFV into cells, suggesting the potential of these compounds as broad-spectrum antivirals targeting the endocytic pathway proteins.
ANTIVIRAL RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Fatima Lasala, Alfonso Garcia-Rubia, Carlos Requena, Inmaculada Galindo, Miguel Angel Cuesta-Geijo, Isabel Garcia-Dorival, Paula Bueno, Nuria Labiod, Joanna Luczkowiak, Ana Martinez, Nuria E. Campillo, Covadonga Alonso, Rafael Delgado, Carmen Gil
Summary: Despite the lack of currently licensed antiviral drugs for treating Ebola virus disease, efforts are ongoing to identify new drugs to combat EBOV. Virtual screening has led to the discovery of small organic molecules with potent inhibitory action against EBOV infection, paving the way for hit to lead optimization programs toward successful candidates.
ANTIVIRAL RESEARCH
(2021)
Article
Virology
Lucia Barrado-Gil, Ana del Puerto, Inmaculada Galindo, Miguel Angel Cuesta-Geijo, Isabel Garcia-Dorival, Carlos Maluquer de Motes, Covadonga Alonso
Summary: The African swine fever virus early protein UBCv1 has been found to modulate innate immune response by targeting NF-kappa B and AP-1 pathways. Its inhibitory activity acts upstream at the level of IKK beta, blocking key events in NF-kappa B signaling. Despite its known ubiquitination capacity, UBCv1's immunomodulatory activity seems to be separate from this function.
Article
Pharmacology & Pharmacy
Isabel Garcia-Dorival, Miguel Angel Cuesta-Geijo, Lucia Barrado-Gil, Inmaculada Galindo, Urtzi Garaigorta, Jesus Urquiza, Ana del Puerto, Nuria E. Campillo, Ana Martinez, Pablo Gastaminza, Carmen Gil, Covadonga Alonso
Summary: The study found a novel interaction between the SARSCoV-2 nucleoprotein (N) and the cholesterol transporter NPC1, and identified compounds that can reduce viral infection, highlighting the importance of NPC1 for SARS-CoV-2 infection and suggesting a potential new therapeutic target against COVID-19.
ANTIVIRAL RESEARCH
(2021)
Article
Microbiology
Miguel Angel Cuesta-Geijo, Isabel Garcia-Dorival, Ana del Puerto, Jesus Urquiza, Inmaculada Galindo, Lucia Barrado-Gil, Fatima Lasala, Ana Cayuela, Carlos Oscar S. Sorzano, Carmen Gil, Rafael Delgado, Covadonga Alonso
Summary: The inner viral membrane proteins of African swine fever virus (ASFV) interact with endosomal proteins, potentially playing a crucial role in the viral entry process. Silencing or knockout of these proteins affects different stages of ASFV infection, and in some cases, prevents the virus from entering the cytoplasm from endosomes. These findings provide new insights into the role of endosomal proteins in ASFV infection.
Article
Biochemistry & Molecular Biology
Maria Angela Oliva, Carlota Tosat-Bitrian, Lucia Barrado-Gil, Francesca Bonato, Inmaculada Galindo, Urtzi Garaigorta, Beatriz Alvarez-Bernad, Rebeca Paris-Ogayar, Daniel Lucena-Agell, Juan Francisco Gimenez-Abian, Isabel Garcia-Dorival, Jesus Urquiza, Pablo Gastaminza, Jose Fernando Diaz, Valle Palomo, Covadonga Alonso
Summary: Microtubule targeting agents have potential in antiviral drug research, and some clinically used drugs have shown significant inhibitory effects on viral infections. However, their mechanism of action is complex and the relationship with intracellular transport is unclear.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Neurosciences
Bruno Hernaez, Abel Viejo-Borbolla, Jorge Ruben Cabrera
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Environmental Sciences
Cristina del Alamo, Angela Vazquez-Calvo, Africa Sanchiz, Gil Rodriguez-Caravaca, Rocio Martin, Bruno Hernaez, Pablo Mendez-Vigo-Carranza, Juan Sanchez Garcia-Casarrubios, Antonio Alcami, Jose Luis Perez-Diaz
Summary: The COVID-19 pandemic has brought attention to the dangers of airborne transmission of pathogens. This study explores a fast method for capturing bioaerosols in air samples, which allows for quick detection of transient levels of aerosols and has revealed the presence of SARS-CoV-2 RNA in hospital rooms. The results show that the distribution of SARS-CoV-2-containing aerosols is not homogeneous, and this technology provides a valuable tool for fast decision-making and better understanding of aerosols dynamics.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2023)
Article
Chemistry, Medicinal
Alfonso Garcia-Rubia, Fatima Lasala, Tiziana Ginex, Marcos Morales-Tenorio, Catherine Olal, Michelle Heung, Paola Oquist, Inmaculada Galindo, Miguel Aprimengel Cuesta-Geijo, Jose M. Casasnovas, Nuria E. Campillo, Angeles Canales, Covadonga Alonso, Ana Martinez, Cesar Munoz-Fontela, Rafael Delgado, Carmen Gil
Summary: Ebola virus (EBOV), a single-strand RNA virus in the Filoviridae family, has been responsible for significant outbreaks since 2013. In this study, a new series of compounds were synthesized and found to inhibit EBOV infection by acting as virus entry inhibitors. The compounds were evaluated for their inhibitory activity against surrogate models and replicative EBOV, and their biological target was elucidated through various experiments. Additionally, their therapeutic potential was confirmed through in vitro metabolic stability and in vivo pharmacokinetic studies.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Ines Giron-Guzman, Azahara Diaz-Reolid, Pilar Truchado, Albert Carcereny, David Garcia-Pedemonte, Bruno Hernaez, Albert Bosch, Rosa Maria Pinto, Susana Guix, Ana Allende, Antonio Alcami, Alba Perez-Cataluna, Gloria Sanchez
Summary: Monkeypox virus (MPXV) DNA was detected in various samples including saliva, semen, urine, and fecal samples besides nasopharyngeal swabs. The study analyzed the presence of MPXV DNA in wastewater collected from different areas of Spain using a surveillance network. The results showed that MPXV DNA could be consistently detected in longitudinal wastewater samples from different treatment plants, indicating the possibility of underestimated cases and frequent asymptomatic infections.
Article
Virology
Isabel Garcia-Dorival, Miguel angel Cuesta-Geijo, Inmaculada Galindo, Ana del Puerto, Lucia Barrado-Gil, Jesus Urquiza, Covadonga Alonso
Article
Infectious Diseases
Bruno Hernaez, Ana Munoz-Gomez, Africa Sanchiz, Eva Orviz, Adrian Valls-Carbo, Inigo Sagastagoitia, Oskar Ayerdi, Rocio Martin, Teresa Puerta, Mar Vera, Noemi Cabello, Jorge Vergas, Cristina Prieto, Maria Pardo-Figuerez, Anabel Negredo, Jose Maria Lagaron, Jorge del Romero, Vicente Estrada, Antonio Alcami
Summary: Monkeypox virus DNA and infectious virus were detected in saliva samples and exhaled droplets and aerosols from patients with monkeypox. Further studies are needed to evaluate the potential relevance of the respiratory route of infection in the 2022 monkeypox virus outbreak.