Article
Biochemistry & Molecular Biology
Ahmad Aljohmani, Bastian Opitz, Markus Bischoff, Daniela Yildiz
Summary: This study found that infection with Pseudomonas aeruginosa and Streptococcus pneumoniae stimulates the activation of ADAM10 in epithelial cells, leading to inflammatory cell recruitment and loss of barrier integrity. The activation is based on the toxin repertoire rather than the bacterial particle itself.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Qiangqiang He, Meiyu Qu, Tingyu Shen, Jiakun Su, Yana Xu, Chengyun Xu, Muhammad Qasim Barkat, Jibao Cai, Haibin Zhu, Ling -Hui Zeng, Ximei Wu
Summary: Mitochondria-associated endoplasmic reticulum membranes (MAMs) are dynamic coupling structures between mitochondria and the endoplasmic reticulum (ER), playing important roles in calcium homeostasis, autophagy, ER stress, lipid metabolism, and various diseases. The formation and function of MAMs rely on specific proteins, including the IP3R-Grp75-VDAC complex. S-palmitoylation, a reversible protein post-translational modification, is closely related to the localization of proteins in MAMs. This article provides an overview of the composition and function of MAMs, focusing on the role of S-palmitoylated proteins in calcium flux, lipid rafts, and discusses their implication in diseases, particularly neurodegenerative diseases (NDs). Potential drug compounds targeting S-palmitoylation are also proposed.
AGEING RESEARCH REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Ludwig Werny, Antonia Grogro, Kira Bickenbach, Cynthia Bulck, Fred Armbrust, Tomas Koudelka, Kriti Pathak, Franka Scharfenberg, Martin Sammel, Farah Sheikhouny, Andreas Tholey, Stefan Linder, Christoph Becker-Pauly
Summary: This study investigated the interaction between membrane-type-I matrix metalloproteinase (MT1-MMP) and meprin beta, revealing a potential regulatory connection between the two enzymes.
Article
Neurosciences
Natsumi Ageta-Ishihara, Sayaka Takemoto-Kimura, Yayoi Kondo, Michiko Okamura, Haruhiko Bito
Summary: CLICK-III/CaMKI? is a lipid-anchored neuronal isoform of Ca2+/calmodulin-dependent protein kinases that mediates BDNF-dependent dendritogenesis. Prenylation and palmitoylation of CaMKI? control its association with detergent-resistant microdomains and are essential for its dendritogenic activity. Prenylation permits membrane anchoring and Golgi access, while palmitoylation facilitates association with cholesterol-enriched lipid microdomains.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Cell Biology
Chenchen Cai, Miaomiao Zhang, Lei Liu, Haoliang Zhang, Yi Guo, Ting Lan, Yinhai Xu, Ping Ma, Shibao Li
Summary: ADAM10 promotes the metastasis of prostate cancer through the shedding of ephrin-A5 and transforming the role of EphA3, and ephrin-A5 in the blood can be used as a new biomarker for PCa metastasis.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Juliane Lokau, Christoph Garbers
Summary: IL-6 and IL-11 are important pleiotropic cytokines that signal through specific receptors, with unique structures. Research has shown that chimeric receptors containing the stalk region of IL-11R can be blocked by a protease inhibitor other than ADAM10 during cleavage.
Article
Cell Biology
Shoji F. Kenji, Keerthi Kurma, Brigitte Collet, Christelle Oblet, Laure Debure, Carmelo Di Primo, Laetitia Minder, Franck Verite, Yannic Danger, Mickael Jean, Aubin Penna, Nicolas Levoin, Patrick Legembre
Summary: CD95 is a death receptor that promotes oncogenesis through various molecular mechanisms. This receptor can undergo cleavage and modification in both cancer cells and normal cells, with the modified N-terminal region potentially regulating receptor aggregation and death signaling.
CELL DEATH & DISEASE
(2022)
Article
Chemistry, Multidisciplinary
Qianmin Ou, Lingping Tan, Yiting Shao, Fangcao Lei, Weiying Huang, Ning Yang, Yan Qu, Zeyuan Cao, Luhan Niu, Yao Liu, Xiaoxing Kou, Songtao Shi
Summary: Mesenchymal stem cell (MSC)-derived apoptotic vesicles (apoVs) can alleviate organ damage and improve survival in septic mice. ApoVs mainly accumulate in the bone marrow of septic mice via electrostatic charge interactions with positively charged neutrophil extracellular traps (NETs). Additionally, apoVs switch neutrophils NETosis to apoptosis via the apoV-Fas ligand (FasL)-activated Fas pathway. These findings reveal a previously unknown role of apoVs in sepsis treatment and demonstrate an electrostatic charge-directed target therapeutic mechanism, suggesting the involvement of cell death in disease development and therapy.
Article
Microbiology
Rajani Ravishankar, Emily R. Hildebrandt, Grace Greenway, Nadeem Asad, Sangram Gore, Timothy M. Dore, Walter K. Schmidt
Summary: CAAX proteins undergo various posttranslational modifications, and this study used a proteolysis-resistant CAAX sequence to investigate Saccharomyces cerevisiae Ras2 GTPase. The results showed that the type of CAAX motif affected Ras2 protein levels, localization, and function.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biology
Mengyang Fan, Wenchao Lu, Jianwei Che, Nicholas P. Kwiatkowski, Yang Gao, Hyuk-Soo Seo, Scott B. Ficarro, Prafulla C. Gokhale, Yao Liu, Ezekiel A. Geffken, Jimit Lakhani, Kijun Song, Miljan Kuljanin, Wenzhi Ji, Jie Jiang, Zhixiang He, Jason Tse, Andrew S. Boghossian, Matthew G. Rees, Melissa M. Ronan, Jennifer A. Roth, Joseph D. Mancias, Jarrod A. Marto, Sirano Dhe-Paganon, Tinghu Zhang, Nathanael S. Gray
Summary: This study reveals the development of covalent TEAD inhibitors and demonstrates their high therapeutic potential for treating cancers driven by TEAD-YAP alteration.
Article
Biochemistry & Molecular Biology
Rolake O. Alabi, Jose Lora, Arda B. Celen, Thorsten Maretzky, Carl P. Blobel
Summary: The Notch signaling pathway relies on the roles of proteins like ADAM10 and ADAM17 to regulate cell fate, with ADAM17 preferring to process Notch1 in ligand-independent conditions, and ADAM10 preferring to process Notch1 in ligand-dependent conditions. These findings provide new insights into the substrate selectivity mechanisms of ADAM10 and ADAM17 towards Notch1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Agnieszka Chytla, Weronika Gajdzik-Nowak, Agnieszka Biernatowska, Aleksander E. Sikorski, Aleksander Czogalla
Summary: Recent studies have highlighted the crucial role of MPP1 in the lateral organization of the erythroid plasma membrane, with palmitoylation playing an important part in this process. An optimized method for high-yield overexpression and purification of palmitoylated recombinant MPP1 protein in mammalian cells has been presented, enabling further exploration of the molecular mechanism and functional impact of MPP1 palmitoylation.
Article
Anatomy & Morphology
Zhengguang Wang, Yongfang Tan, Fei Xu
Summary: This study aimed to investigate the effects of zonisamide (ZNS) on a degenerative cervical myelopathy (DCM) rat model and explore the potential mechanisms. The results showed that ZNS promoted motor recovery, reversed histopathological injury and cell apoptosis in DCM rats. Additionally, ZNS attenuated DCM injury through the regulation of Fas and FasL signaling. Therefore, this study provided a new theoretical approach for subsequent academic and clinical research on DCM injury.
Article
Allergy
Maria Elena Maccari, Pascal Schneider, Cristian Roberto Smulski, Andrea Meinhardt, Fernando Pinto, Luis Ignacio Gonzalez-Granado, Catharina Schuetz, Mauricio Pablo Sica, Miriam Gross, Ilka Fuchs, Patrick Kury, Maximilian Heeg, Tatjana Vocat, Laure Willen, Caroline Thomas, Regina Huehn, Aude Magerus, Myriam Lorenz, Klaus Schwarz, Frederic Rieux-Laucat, Stephan Ehl, Anne Rensing-Ehl
Summary: Heterozygous loss-of-function mutations in FASLG are better tolerated than in FAS, and usually do not result in expanded double-negative T cells (DNT) and elevated serum vitamin B12 levels, which are characteristic of autoimmune-lymphoproliferative syndrome (ALPS).
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2023)
Article
Immunology
Sean Hasan, Belal Alshaikh, Kamran Yusuf
Summary: Our study reveals higher levels of sFasL in pregnant women who smoke, potentially reducing the incidence of preeclampsia by inducing apoptosis of immune cells.
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Justin Ferdinandus, Wolfgang P. Fendler, Katharina Lueckerath, Christoph Berliner, Sabine Kurzidem, Eva Hadaschik, Joachim Klode, Lisa Zimmer, Elisabeth Livingstone, Dirk Schadendorf, Ken Herrmann, Juergen C. Becker, Selma Ugurel
Summary: For patients with Merkel cell carcinoma (MCC) who are refractory to immune checkpoint inhibition (ICI), treatment options are limited. Combination therapy with peptide receptor radionuclide therapy (PRRT) and immune checkpoint inhibitors (ICI) may be an effective treatment option.
JOURNAL OF NUCLEAR MEDICINE
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Jasmin M. Klose, Jasmin Wosniack, Janette Iking, Magdalena Staniszewska, Fadi Zarrad, Marija Trajkovic-Arsic, Ken Herrmann, Pedro Fragoso Costa, Katharina Lueckerath, Wolfgang P. Fendler
Summary: The study assessed the biodistribution of various radioligand application routes in mice, and found that subcutaneous and intraperitoneal administration had comparable biodistribution to intravenous injection. Subcutaneous administration resulted in the highest tumor uptake, suggesting its potential in clinical assessment.
JOURNAL OF NUCLEAR MEDICINE
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Lukas Kessler, Justin Ferdinandus, Nader Hirmas, Fadi Zarrad, Michael Nader, David Kersting, Manuel Weber, Sandra Kazek, Miriam Sraieb, Rainer Hamacher, Katharina Lueckerath, Lale Umutlu, Wolfgang P. Fendler, Christoph Rischpler
Summary: This study summarizes common findings and potential pitfalls in Ga-68-FAPI PET/CT. Common findings include uptake in degenerative lesions and head-and-neck. Pitfalls include non-tumor-specific uptake in degenerative lesions, muscle, scarring, mammary glands, or uterus.
JOURNAL OF NUCLEAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Mathias Lechelon, Yoann Meriguet, Matteo Gori, Sandra Ruffenach, Ilaria Nardecchia, Elena Floriani, Dominique Coquillat, Frederic Teppe, Sebastien Mailfert, Didier Marguet, Pierre Ferrier, Luca Varani, James Sturgis, Jeremie Torres, Marco Pettini
Summary: In this study, the activation of resonant electrodynamic intermolecular forces was experimentally demonstrated using two independent experiments based on different physical effects detected by fluorescence correlation spectroscopy and terahertz spectroscopy, respectively. This discovery has significant implications for our understanding of biomolecular interactions and cellular dynamics.
Article
Oncology
Wolfgang P. Fendler, Kim M. Pabst, Lukas Kessler, Pedro Fragoso Costa, Justin Ferdinandus, Manuel Weber, Maria Lippert, Katharina Lueckerath, Lale Umutlu, Karina Kostbade, Ilektra A. Mavroeidi, Martin Schuler, Marit Ahrens, Christoph Rischpler, Sebastian Bauer, Ken Herrmann, Jens T. Siveke, Rainer Hamacher
Summary: This study reports the efficacy and safety of 90Y-labeled FAPI-46 in patients with advanced sarcoma, pancreatic cancer, and other cancers. The results show disease control in approximately 38% of patients, with a prolonged overall survival associated with disease control. However, there were adverse events observed, with thrombocytopenia and anemia being the most common.
CLINICAL CANCER RESEARCH
(2022)
Article
Oncology
Anna Boegelein, Antje Stolzenburg, Patrick Eiring, Katharina Luckerath, Umair Munawar, Rudolf Werner, Andreas Schirbel, Samuel Samnick, Klaus Martin Kortuem, Markus Sauer, Constantin Lapa, Andreas K. Buck
Summary: CXCR4 expression is highly variable and can be influenced by anti-myeloma drugs. Different drugs and doses have different effects on CXCR4 expression, with some drugs reducing expression while others increasing it. Combination of multiple drugs can further increase CXCR4 expression.
LEUKEMIA & LYMPHOMA
(2022)
Meeting Abstract
Oncology
R. W. Hamacher, K. M. Pabst, L. Kessler, P. F. Costa, J. Ferdinandus, M. Weber, M. Lippert, K. Lueckerath, N. Hirmas, K. Kostbade, I. A. Mavroeidi, M. H. H. Schuler, M. Ahrens, C. Rischpler, S. Bauer, K. Herrmann, J. T. Siveke, W. P. Fendler
ANNALS OF ONCOLOGY
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Catherine Meyer, Vikas Prasad, Andreea Stuparu, Peter Kletting, Gerhard Glatting, Jonathan Miksch, Christoph Solbach, Katharina Lueckerath, Lea Nyiranshuti, Shaojun Zhu, Johannes Czernin, Ambros J. Beer, Roger Slavik, Jeremie Calais, Magnus Dahlbom
Summary: This study compared the biodistribution of PSMA-TO-1, PSMA-617, and PSMA-11 in mice and prostate cancer patients, and evaluated their therapeutic effects and dosimetry data. The results showed that PSMA-TO-1 had higher tumor uptake and better treatment efficacy in mice. However, PSMA-TO-1 also led to increased uptake and doses in organs such as the kidneys.
Article
Radiology, Nuclear Medicine & Medical Imaging
Nader Hirmas, Rainer Hamacher, Miriam Sraieb, Marc Ingenwerth, Lukas Kessler, Kim M. Pabst, Francesco Barbato, Katharina Lueckerath, Stefan Kasper, Michael Nader, Hans-Ulrich Schildhaus, Claudia Kesch, Bastian von Tresckow, Christine Hanoun, Hubertus Hautzel, Clemens Aigner, Martin Glas, Martin Stuschke, Sherko Kummel, Philipp Harter, Celine Lugnier, Waldemar Uhl, Marco Niedergethmann, Boris Hadaschik, Viktor Grunwald, Jens T. Siveke, Ken Herrmann, Wolfgang P. Fendler
Summary: This article presents an overview of a prospective registry study on fibroblast-activation protein inhibitor (FAPI) over a 3-year period, comparing tumor uptake in 68Ga-FAPI and 18F-FDG PET as well as FAP immunohistochemistry. The study found that 68Ga-FAPI had higher tumor uptake in pancreatic cancer and sarcoma compared to 18F-FDG, and also demonstrated improved tumor detection.
JOURNAL OF NUCLEAR MEDICINE
(2023)
Article
Oncology
Claire M. Storey, Mohamed Altai, Mesude Bicak, Darren R. Veach, Katharina Lueckerath, Gabriel Adrian, Michael R. McDevitt, Teja Kalidindi, Julie E. Park, Ken Herrmann, Diane Abou, Wahed Zedan, Norbert Peekhaus, Robert J. Klein, Robert Damoiseaux, Steven M. Larson, Hans Lilja, Daniel Thorek, David Ulmert
Summary: Noninvasive biomarkers for androgen receptor (AR) pathway activation are urgently needed to better monitor patient response to prostate cancer therapies. A novel PET modality targeting hK2 was found to accurately assess EBRT-induced AR activity, which can be used for monitoring treatment response and development of resistance.
MOLECULAR CANCER RESEARCH
(2023)
Meeting Abstract
Oncology
Caius G. Radu, Kenneth A. Schultz, Katharina Luckerath, H. Michael Shepard, Johannes Czernin
Meeting Abstract
Radiology, Nuclear Medicine & Medical Imaging
N. Hirmas, M. Ingenwerth, M. Sraieb, L. Kessler, K. M. Pabst, H. Lanzafame, K. Lueckerath, S. Kasper, M. Nader, H. Schildhaus, B. Hadaschik, V. Gruenwald, B. Von Tresckow, C. Hanoun, C. Aigner, M. Glas, K. Herrmann, J. T. Siveke, R. Hamacher, W. P. Fendler
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2022)
Meeting Abstract
Radiology, Nuclear Medicine & Medical Imaging
C. Civan, S. Kasper, C. Berliner, P. Fragoso-Costa, V. Gruenwald, M. Pogorzelski, B. M. Schaarschmidt, S. Lang, D. Kersting, M. Nader, K. Lueckerath, K. Herrmann, W. P. Fendler, M. Weber
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2022)
Meeting Abstract
Radiology, Nuclear Medicine & Medical Imaging
W. Fendler, K. M. Pabst, L. Kessler, P. F. Costa, J. Ferdinandus, M. Weber, M. Lippert, K. Lueckerath, N. Hirmas, K. Kostbade, A. Mavroeidi, M. Schuler, M. Ahrens, C. Rischpler, S. Bauer, K. Herrmann, J. T. Siveke, R. Hamacher
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2022)
Meeting Abstract
Radiology, Nuclear Medicine & Medical Imaging
K. M. Pabst, W. P. Fendler, B. Hadaschik, C. Kesch, N. Hirmas, L. Kessler, V. Gruenwald, R. Hamacher, M. Nader, C. Berliner, L. Umutlu, L. Puellen, K. Herrmann, K. Lueckerath, P. Castellucci, S. Fanti, R. Mei
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2022)